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Zhong Y.,Shanghai JiaoTong University | Zhao J.,Jin Shan Branch Hospital of Sixth Peoples Hospital of Shanghai | Gu Y.-J.,Jin Shan Branch Hospital of Sixth Peoples Hospital of Shanghai | Zhao Y.-F.,Jin Shan Branch Hospital of Sixth Peoples Hospital of Shanghai | And 2 more authors.
Archives of Gerontology and Geriatrics | Year: 2015

Objective: Most publications describe cathepsin B and L as tumor and metastasis factors. These proteases also play a very important role in aging process. The aim of this study was to evaluate the serum level of cathepsin B and L with aging and their association with matrix metalloproteinase 2 (MMP2), which was reported to associate with age-related diseases. Methods: This research was conducted using blood samples provided by healthy people (n= 90, 63 men and 27 women). Subjects were subdivided into groups with respect to age: young (about 18-30 years old, n= 30), middle age (about 36-50 years old, n= 30), and aged (above 56 years old, n= 30). Altered serum level of cathepsin B, cathepsin L, and MMP2 with aging was studied by enzyme-linked immunosorbent assay (ELISA) and Western blotting using discriminative antibodies specific for each factor. Results: ELISA and Western blotting revealed that the serum level of cathepsin L and MMP2, but not cathepsin B significantly decreased in aged group compared with young group. Cathepsin L positively correlates with MMP2 among the whole healthy people (r2=0.869, p<0.0001). Conclusion: The serum level of cathepsin L decreased with age, while cathepsin B remained no significant difference between young and aged individuals. In addition, cathepsin L positively correlates with MMP2. Practice: The cathepsin L may be used as a monitoring index in age-related diseases. Implications: In addition to cathepsin B, cathepsin L may be also involved in the aging process. © 2015 Elsevier Ireland Ltd.

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