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Bai L.,Peoples Hospital of Sichuan Province | Loo W.T.,UNIMED Medical Institute | Loo W.T.,Chinese University of Hong Kong | Dou Y.,Jin Hua Dentistry | And 4 more authors.
African Journal of Biotechnology | Year: 2011

Inflammation is an important event in the development of vascular diseases such as hypertension, atherosclerosis, and restenosis. The stimulation of lipopolysaccharide (LPS) from bacteria induces the release of critical proinflammatory cytokines that activate potent immune responses which may cause injury of cells in vivo and in vitro. Upon cardiac cell death caused by inflammation, the apoptotic cardiac cells express higher amount of cardiac markers. In this study, the effect of various LPS on human cardiac fibroblasts (HCFs) and human coronary smooth muscle cells (HCSMCs) were evaluated. Various forms of LPS were applied to HCFs and HCSMCs for 24, 48, 72 and 96 h. Proliferation rate of these cells was evaluated after stimulation. The levels of lactate dehydrogenase (LDH), N-terminal pro B-type natriuretic peptide (pro-BNP) and the MB isoenzyme of creatine kinase (CK-MB) were measured by an automation system. Cytokine levels in culture supernatants and extracted protein of cells were mixed and measured with IL-1β, IL-6 and IL-10 ELISA kits. Significant increase in the proliferation of two cardiac cells (P<0.05) after incubation for 48 and 72 h was noted but not for 24 and 96 h (P>0.05). Cardiac markers and inflammatory cytokines were significantly higher than control at 48 and 72 h (P<0.05), which demonstrated that HCFs and HCMSCs were under inflammation leading to cell injury between 48 and 72 h. LPS is one of the factors giving rise to periodontal diseases, it is also involved in in vitro cardiac cell injury. Therefore, LPS may be used as a bio-marker to monitor local or systemic inflammation. © 2011 Academic Journals.

Dou D.,Chengdu Family Planning Guidance Institute | Tao W.,Chengdu Family Planning Guidance Institute | Li L.,Chengdu Family Planning Guidance Institute | Jia T.,Chengdu Family Planning Guidance Institute | And 5 more authors.
Journal of Medicinal Plants Research | Year: 2011

Taxol has anti-tumor properties, polysaccharide peptide (PSP), an active substance of Yunzhi, is an effective immunopotentiator, which is used to supplement the chemotherapy and radiotherapy of cancers patients. The antitumor activity of polysaccharopeptides has been documented. In this study, the in vitro effect of PSP upon the metabolic rate of breast solid tumors was observed and this effect was compared with taxol, which is a well-known chemotherapeutic drug. 117 patients' tissues were treated with 4, 2 and 1 mg/ml of PSP and 4.27 μg/ml of taxol for 24 h. ATP bioluminescence assay was used to measure the in vitro metabolic rate of the breast cancer tissues and SPSS was used for statistical analysis. The estrogen receptor (ER), progesterone receptor (PR), HER-2, tumor grading and the age of patients were all analysed in the study. Taxol was significantly effective on tumors in younger aged, low tumor grade; ER and PR negative group's subjects. PSP demonstrated similar results and also suppresses the activity of breast solid tumor. ATP bioluminescence assay was successfully performed in determining tumor metabolic rate in a timely fashion such that chemotherapeutic treatment could be guided by the results. © 2011 Academic Journals.

Yue Y.,University of Sichuan | Liu Q.,UNIMED Medical Institute and Organisation for Oncology and Translational Research | Xu C.,Central South University | Loo W.T.Y.,UNIMED Medical Institute and Organisation for Oncology and Translational Research | And 11 more authors.
International Journal of Biological Markers | Year: 2013

Objectives: This study aims to evaluate and compare cytokines in gingival crevicular fluid (GCF) and saliva of patients with aggressive periodontitis (AP) before and after treatment. Methods: Forty AP patients and 40 healthy volunteers were enrolled in this study. Clinical parameters included probing depth and sulcus bleeding index. GCF and saliva were collected from both groups. The levels of IL-1β, IL-2, IL-4, IL-6, IFN-γ and TNF-α were measured using ELISA. Results: The probing depth in AP patients was significantly deeper before treatment than after treatment. The concentrations of cytokines in GCF and saliva were significantly higher in AP patients than in the control group and decreased after periodontal treatment. Positive relationships were found between cytokine levels in GCF and clinical parameters. The reliability of cytokines in GCF and saliva was assessed by Cronbach's alpha analysis, which could be considered satisfactory. Conclusion: Cytokine levels in GCF and saliva correlated well with clinical parameters and AP. Measurements of cytokines in saliva may be regarded as a noninvasive and quick method for monitoring periodontal disease activity. © 2013 Wichtig Editore.

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