Jin Hua Dentistry

Chengdu, China

Jin Hua Dentistry

Chengdu, China
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Dou D.,Chengdu Family Planning Guidance Institute | Tao W.,Chengdu Family Planning Guidance Institute | Li L.,Chengdu Family Planning Guidance Institute | Jia T.,Chengdu Family Planning Guidance Institute | And 6 more authors.
African Journal of Pharmacy and Pharmacology | Year: 2011

Thymosin-alpha-1 (Tα1), a synthetic thymus hormone, can increase the production of T cells. It may counteract the suppressed immunity of chemotherapy patients, thus restoring normal immune mechanism. This study aims to investigate the effect of thymosin-alpha-1 on the post-operation conditions of patients who have received modified radical mastectomy (MRM). Thirty-six consented patients with invasive breast ductal carcinoma (IDC) who were to receive MRM were randomly divided into two groups. One received hormonal therapy and the other received chemotherapy (four cycles of 5- fluorouracil, epirubicin and cyclophosphamide (FEC)) at least three weeks before surgery. Tα1 was administered in both groups, one day before surgery till day seven after surgery. Post-surgical inflammatory and metabolic responses (heart rate, wound area, body temperature, pain level, wound drainage and cytokine levels) were analyzed. Amount of drainage and cytokine levels did not show any significant difference between the groups. Temperature on Day 2 and the overall pain levels of the hormonal therapy group were significantly higher than that in the chemotherapy group. There was no significant difference in wound areas or heart rates between the two groups. Tα1 normalizes immune response parameters among chemotherapy patients to close-to-normal levels, while at the same time significantly depress level of pain, as compared to hormonal therapy-treated patients. Amount of drainage, heart rate and cytokine levels do not show any significant difference between the groups, indicating that Tα1 has normalized the metabolic and immune response of the chemotherapy patients. © 2011 Academic Journals.

Bai L.,Peoples Hospital of Sichuan Province | Loo W.T.,UNIMED Medical Institute | Loo W.T.,Chinese University of Hong Kong | Dou Y.,Jin Hua Dentistry | And 4 more authors.
African Journal of Biotechnology | Year: 2011

Inflammation is an important event in the development of vascular diseases such as hypertension, atherosclerosis, and restenosis. The stimulation of lipopolysaccharide (LPS) from bacteria induces the release of critical proinflammatory cytokines that activate potent immune responses which may cause injury of cells in vivo and in vitro. Upon cardiac cell death caused by inflammation, the apoptotic cardiac cells express higher amount of cardiac markers. In this study, the effect of various LPS on human cardiac fibroblasts (HCFs) and human coronary smooth muscle cells (HCSMCs) were evaluated. Various forms of LPS were applied to HCFs and HCSMCs for 24, 48, 72 and 96 h. Proliferation rate of these cells was evaluated after stimulation. The levels of lactate dehydrogenase (LDH), N-terminal pro B-type natriuretic peptide (pro-BNP) and the MB isoenzyme of creatine kinase (CK-MB) were measured by an automation system. Cytokine levels in culture supernatants and extracted protein of cells were mixed and measured with IL-1β, IL-6 and IL-10 ELISA kits. Significant increase in the proliferation of two cardiac cells (P<0.05) after incubation for 48 and 72 h was noted but not for 24 and 96 h (P>0.05). Cardiac markers and inflammatory cytokines were significantly higher than control at 48 and 72 h (P<0.05), which demonstrated that HCFs and HCMSCs were under inflammation leading to cell injury between 48 and 72 h. LPS is one of the factors giving rise to periodontal diseases, it is also involved in in vitro cardiac cell injury. Therefore, LPS may be used as a bio-marker to monitor local or systemic inflammation. © 2011 Academic Journals.

Dou D.,Chengdu Family Planning Guidance Institute | Tao W.,Chengdu Family Planning Guidance Institute | Li L.,Chengdu Family Planning Guidance Institute | Jia T.,Chengdu Family Planning Guidance Institute | And 5 more authors.
Journal of Medicinal Plants Research | Year: 2011

Taxol has anti-tumor properties, polysaccharide peptide (PSP), an active substance of Yunzhi, is an effective immunopotentiator, which is used to supplement the chemotherapy and radiotherapy of cancers patients. The antitumor activity of polysaccharopeptides has been documented. In this study, the in vitro effect of PSP upon the metabolic rate of breast solid tumors was observed and this effect was compared with taxol, which is a well-known chemotherapeutic drug. 117 patients' tissues were treated with 4, 2 and 1 mg/ml of PSP and 4.27 μg/ml of taxol for 24 h. ATP bioluminescence assay was used to measure the in vitro metabolic rate of the breast cancer tissues and SPSS was used for statistical analysis. The estrogen receptor (ER), progesterone receptor (PR), HER-2, tumor grading and the age of patients were all analysed in the study. Taxol was significantly effective on tumors in younger aged, low tumor grade; ER and PR negative group's subjects. PSP demonstrated similar results and also suppresses the activity of breast solid tumor. ATP bioluminescence assay was successfully performed in determining tumor metabolic rate in a timely fashion such that chemotherapeutic treatment could be guided by the results. © 2011 Academic Journals.

Xu C.,Central South University | Zhao J.,Chinese University of Hong Kong | Loo W.T.Y.,Chinese University of Hong Kong | Loo W.T.Y.,UNIMED Medical Institute and Organisation for Oncology and Translational Research | And 8 more authors.
International Journal of Biological Markers | Year: 2012

Background: DNA methylation of certain genes is an epigenetic change that is essential for tumorigenesis. Oral submucous fibrosis (OSF) is a precancerous condition of oral mucosa with inflammation and progressive fibrosis of the lamina propria and deeper connective tissue. The hypermethylation of E-cadherin and cyclooxygenase 2 (COX-2) in chronic inflammation may demonstrate a mild lesion/mutation at epigenetic levels. This study compares the hypermethylation status of E-cadherin and COX-2 genes in patients with oral cancer and patients with OSF and also aims to identify risk factors for the development of OSF. Methods: DNA was extracted from blood samples of 50 healthy subjects, 50 patients with OSF and 60 patients with oral cancer. Methylation-specific polymerase chain reaction for E-cadherin and COX-2 was performed on these samples and the products were analyzed on 2% agarose gel. Surveys about oral health habits and clinical periodontal examinations in patients with OSF and healthy subjects were also conducted by well-trained dentists, and logistic regression was performed to identify risk factors for OSF. Results: Hypermethylation of E-cadherin and COX-2 was observed in 36% and 22% of oral cancer samples, respectively. In patients with OSF, the rates were 52% and 30%, and in healthy controls the rates were 4% and 6%. Hypermethylation was shown to be correlated between the 3 groups with statistical significance (p<0.01). Methylation of CpG islands in E-cadherin and COX-2 occurred more frequently in patients with OSF than in the control group, but less frequently than in patients with oral cancer. In the logistic regression analysis, smoking, brushing more than twice daily, periodontal probing depth and plaque index were identified as 4 major risk factors for OSF. Conclusions: These data confirm that E-cadherin and COX-2 expressions are related to OSF. The epigenetic changes presented in patients with chronic inflammation might demonstrate an irreversible destruction in the tissues or organs similar to the effects of cancer. Chronic OSF was significantly associated with hypermethylation, a cancer risk factor. © 2012 Wichtig Editore - ISSN 0393-6155.

Yue Y.,University of Sichuan | Liu Q.,UNIMED Medical Institute and Organisation for Oncology and Translational Research | Xu C.,Central South University | Loo W.T.Y.,UNIMED Medical Institute and Organisation for Oncology and Translational Research | And 11 more authors.
International Journal of Biological Markers | Year: 2013

Objectives: This study aims to evaluate and compare cytokines in gingival crevicular fluid (GCF) and saliva of patients with aggressive periodontitis (AP) before and after treatment. Methods: Forty AP patients and 40 healthy volunteers were enrolled in this study. Clinical parameters included probing depth and sulcus bleeding index. GCF and saliva were collected from both groups. The levels of IL-1β, IL-2, IL-4, IL-6, IFN-γ and TNF-α were measured using ELISA. Results: The probing depth in AP patients was significantly deeper before treatment than after treatment. The concentrations of cytokines in GCF and saliva were significantly higher in AP patients than in the control group and decreased after periodontal treatment. Positive relationships were found between cytokine levels in GCF and clinical parameters. The reliability of cytokines in GCF and saliva was assessed by Cronbach's alpha analysis, which could be considered satisfactory. Conclusion: Cytokine levels in GCF and saliva correlated well with clinical parameters and AP. Measurements of cytokines in saliva may be regarded as a noninvasive and quick method for monitoring periodontal disease activity. © 2013 Wichtig Editore.

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