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Takasaki, Japan

Yazawa S.,Gunma University | Yazawa S.,Otsuka Pharmaceutical Factory Inc. | Yokobori T.,Gunma University | Ueta G.,Nagaoka University of Technology | And 14 more authors.
PLoS ONE | Year: 2014

Blood group-related glycans determining ABO and Lewis blood groups are known to function as attachment factors for most of the norovirus (NoV) strains. To identify binding specificity of each NoV, recombinant norovirus-like particles (VLPs) and human saliva samples with different ABO, Lewis phenotypes and secretor status have been commonly applied. When binding specificities of VLPs prepared from 16 different genotypes of NoVs in GI and GII genogroups were characterized in samples of human gastric mucosa compared to human saliva based on blood group phenotypes, considerable differences were observed for several strains. Novel binding specificities determined by an ELISA using preparations from human gastric mucosa were also ascertained by immunohistochemical analyses using human jejunal mucosa, widely believed to be susceptible to NoV infection. Further, A, B and O(H) blood group substances prepared from porcine and squid tissues were found to be effective for preventing ABO blood group-specific binding of VLPs to both saliva and mucosa samples. Therefore, these blood group substances might have potential for the prevention and treatment of NoV infection. © 2014 Yazawa et al.

Asao T.,Gunma University | Yazawa S.,Gunma University | Yazawa S.,Otsuka Pharmaceutical Factory Inc. | Nishimura T.,Otsuka Pharmaceutical Factory Inc. | And 4 more authors.
BioMed Research International | Year: 2013

Human plasma α1-acid glycoprotein (AGP) from cancer patients and healthy volunteers was purified by sequential application of ion-exchange columns, and N-linked glycans enzymatically released from AGP were labeled and applied to a mass spectrometer. Additionally, a novel software system for use in combination with a mass spectrometer to determine N-linked glycans in AGP was developed. A database with 607 glycans including 453 different glycan structures that were theoretically predicted to be present in AGP was prepared for designing the software called AGPAS. This AGPAS was applied to determine relative abundance of each glycan in the AGP molecules based on mass spectra. It was found that the relative abundance of fucosylated glycans in tri- and tetra-antennary structures (FUCAGP) was significantly higher in cancer patients as compared with the healthy group (P<0.001). Furthermore, extremely elevated levels of FUCAGP were found specifically in patients with a poor prognosis but not in patients with a good prognosis. In conclusion, the present software system allowed rapid determination of the primary structures of AGP glycans. The fucosylated glycans as novel tumor markers have clinical relevance in the diagnosis and assessment of cancer progression as well as patient prognosis. © 2013 Takayuki Asao et al.

Shi M.,Kansai Medical University | Adachi Y.,Kansai Medical University | Cui Y.,Kansai Medical University | Cui Y.,JIMRO Co. | And 7 more authors.
Stem Cells and Development | Year: 2011

The combination of allogeneic bone marrow transplantation (allo-BMT) and donor lymphocyte infusion (DLI) is a useful method for establishing donor chimerism and preventing a relapse of leukemia/lymphoma. However, there is a risk of inducing uncontrollable fatal graft-versus-host disease (GVHD). In fact, allo-BMT plus intravenous (IV)-DLI using donor splenocytes induces fatal GVHD in recipient mice. In this study, we examined the effects of the combination of intra-bone marrow (IBM)-BMT and the subcutaneous injection of donor splenocytes (SC-DLI) on the allo-BMT system. Recipient BALB/c mice were conditioned by sublethal irradiation (5 Gy), followed by IBM-BMT plus IV-DLI or SC-DLI in C57BL/6 mice. The IV-DLI group showed better engraftment of donor hemopoietic cells than the control group (without DLI) but showed fatal GVHD. The SC-DLI group, however, showed good reconstitution and mild GVHD. These results suggest that the combination of SC-DLI and IBM-BMT promotes the reconstitution of hemopoiesis and helps reduce the risk of GVHD. © Copyright 2011, Mary Ann Liebert, Inc.

Zhang Y.,Kansai Medical University | Hosaka N.,Kansai Medical University | Cui Y.,Kansai Medical University | Cui Y.,JIMRO Co. | And 2 more authors.
Stem Cells and Development | Year: 2011

We have recently shown that allogeneic intrabone marrow-bone marrow transplantation+adult thymus transplantation (TT) is effective for hosts with malignant tumors. However, since thymic and hematopoietic cell functions differ with age, the most effective age for such intervention needed to be determined. We performed hematopoietic stem cell transplantation (HSCT) using the intrabone marrow method with or without TT from fetal, newborn, and adult B6 mice (H-2b) into BALB/c mice (H-2d) bearing Meth-A sarcoma (H-2d). The mice treated with all types of HSCT+TT showed more pronounced regression and longer survival than those treated with HSCT alone in all age groups. Those treated with HSCT+TT showed increased numbers of CD4 + and CD8+ T cells but decreased numbers of Gr-1/Mac-1 myeloid suppressor cells and decreased percentages of FoxP3 cells in CD4 + T cells, compared with those treated with HSCT alone. In all mice, those treated with fetal liver cell (as fetal HSCs) transplantation+fetal TT or with newborn liver cell (as newborn HSCs) transplantation (NLT)+newborn TT (NTT) showed the most regression, and the latter showed the longest survival. The number of Gr-1/Mac-1 cells was the lowest, whereas the percentage of CD62L -CD44+ effector memory T cells and the production of interferon γ (IFN-γ) were highest in the mice treated with NLT+NTT. These findings indicate that, at any age, HSCT+TT is more effective against cancer than HSCT alone and that NLT+NTT is most effective. © Copyright 2011, Mary Ann Liebert, Inc. 2011.

Feng W.,Kansai Medical University | Feng W.,Shanghai University of Traditional Chinese Medicine | Cui Y.,Kansai Medical University | Cui Y.,JIMRO Co. | And 11 more authors.
Transplantation | Year: 2010

Background: Two side effects of irradiation are premature ovarian failure (POF) and osteoporosis, both of which are concerns not only clinically, for patients, but also experimentally, for animals. We examine whether bone marrow transplantation (BMT) can correct the POF induced by radiation and also address whether allogeneic ovarian transplantation (OT) can modulate the adverse effects of radiotherapy. Methods: Eight-week-old female C57BL/6 mice were lethally irradiated with 6 Gy ×2, and then injected with allogeneic bone marrow cells into their bone marrow cavity using our previously described intrabone marrow (IBM)-BMT technique. Allogeneic ovaries were simultaneously transplanted under the renal capsules of the mice. Results: Three months after the transplantation, we noted that hematopoietic and lymphoid cells had been successfully reconstituted. The ovaries transplanted under the renal capsules demonstrated signs of development with a large number of differentiating follicles at different stages of development. Importantly, the total bone mineral density of the tibia in the "IBM-BMT+OT" (BMT/OT) group remained normal. However, the reproductive function of the recipient mice was not restored, despite the presence of many immature oocytes in the host ovaries in the BMT/OT group. In the BMT group, no oocytes were found in the host ovaries. ConclusionS: These findings suggest that IBM-BMT with ovarian allografts can be advantageous for young women with POF and osteopenia or osteoporosis that is due to chemotherapy and radiotherapy for malignant diseases. Copyright © 2010 by Lippincott Williams & Wilkins.

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