Wang D.,Jilin University |
Wang G.,Jilin University |
Yang H.,Jilin University |
Liu H.,JilinUniversity |
And 6 more authors.
Genetics and Molecular Biology | Year: 2017
The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with H19 DMRs, IGF2 DMRs showed a different methylation pattern in the eye. Taken together, these results suggest that elevated expression of H19/IGF2 is caused by a specific chromatin structure that is regulated by the DNA methylation status of IGF2 DMRs in the female eye. © 2017, Sociedade Brasileira de Genética.
Tian L.M.,JilinUniversity |
Wang Y.C.,JilinUniversity |
Gao Z.H.,JilinUniversity |
Bu Z.G.,Wuxi Fuel Injection Equipment Research Institute |
Applied Mechanics and Materials | Year: 2014
Some living creatures have special structures on their body surfaces, such as smooth and elastic epidermis with subcutaneous tissue having non-smooth structures under certain conditions. The elastic epidermis coupled with non-smooth structures has a special function called bio-coupling functional surface. Imitating this functional surface and applying it in engineering has a potential to solve some engineering problems. Based on the simulation method of fluid-structure interaction (FSI), simulation calculation of the bionic functional surface coupled by the two factors, form and flexible materials was conduct using ADINA software. A viscous and weakly compressible transient flow was selected as a working medium, a discrete solver was selected in numerical calculation and the basic κ - ε model was chosen as a turbulence model. It is assumed that the coupling surface of the form/flexible materials results is large deformation and large strain. The boundary condition of fluid-structure interaction was set as the calculation surface. The simulation results showed that this coupling is a dynamic process, in which the two factors (form and flexible materials) are influenced by the flow field. As the pressure and velocity of the flow field increase, the coupling process changes from partial coupling to complete coupling, the pressure drag decreased due to the maximum effective stress of bionic coupling surface is very small and the smooth and flexible materials can redistribute pressure by absorbing and releasing energy, the pressure drag thus formed is decreased. Moreover, non-smooth structures (form factor) coupled with flexible materials reduced velocity of working face and minimise energy losses effectively, enabling the bionic coupling surface to reduce drag. © (2014) Trans Tech Publications, Switzerland.
Xu H.-J.,JilinUniversity |
Xu H.-J.,Jilin Medical College |
Du T.-H.,Jilin University |
Sun Y.,JilinUniversity |
And 2 more authors.
Journal of Jilin University Medicine Edition | Year: 2014
Objective: To construct the eukaryotic expression vectors of fibroblast growth factor receptor 3(FGFR3) MSCV/puro-fgfr3-WT and MSCV/puro-fgfr3-DN, and to detect their expressions in human chronic myeloid leukemia(CML) K562 cell line. Methods: The full-length FGFR3 (fgfr3-WT) and dominant negative FGFR3 (fgfr3-DN) were amplified by polymerase chain reaction (PCR). The two genes were respectively digested with EcoR I and BamH I, and then ligated into MSCV/puro to construct the recombinant plasmids MSCV/puro-fgfr3-WT and MSCV/puro-fgfr3-DN which were tranduced into K562 cells by Lipofectamin™ 2000 after PCR, double digestion and DNA sequencing. The expressions of FGFR3 protein in K562 cells were detected by Western blotting and flow cytometry. Results: The recombinant plasmids MSCV/puro-fgfr3-WT and MSCV/puro-fgfr3-DN were amplified by PCR method, and the results showed fgfr3-WT of 2 400 bp and fgfr3-DN of 1 200 bp had been successfully cloned into MSCV-puro vector. The 2 400 bp fragment was obtained after double digestion of recombinant plasmid. The sequencing results showed that the size of fgfr3-WT was 2 400 bp which was the same as the sequence from GeneBank. Fgfr3-DN of 1 200 bp was also in conformity with the expected sequence. Compared with control (K562 MSCV) group, the expression level of FGFR3-WT in MSCV/puro-fgfr3-WT transfection (K562-WT) group was increased to above 10 times. There was high expression of FGFR3-DN in MSCV/puro-FGFR3-DN transfection (K562-DN) group, but there was no expressions in control (K562 MSCV) group and K562-WT group. The flow cytometry results showed that the high expressions of FGFR3-WT were in 57.5% cells in K562-DN group and the high expressions of FGFR3-DN were in 41.5% cells in K562-DN group. Conclusion: The K562 cell lines highly expressing FGFR3-WT and FGFR3-DN are constructed successfully.
Chen N.,JilinUniversity |
Ma N.,JilinUniversity |
Che H.,JilinUniversity |
Jiao P.,JilinUniversity |
And 2 more authors.
Journal of Jilin University Medicine Edition | Year: 2017
Objective: To use polylactic-co-glycolic acid (PLGA) as vector material to prepare the compound total flavonoids of Rhizoma Drynariae (TFRD) and total flavonoids of Chrysanthemum (TFC) sustained release microcapsule TF-PLGA microcapsules, and to investigate the the best preparation technique of TF-PLGA microcapsules and their sustained release characteristics in vitro. Methods: The TF-PLGA microcapsules were prepared with TFRD, TFC, and PLGA by emulsifying-solvent evaporation technique under certain conditions. With the encapsulation efficiency (EE) as the evaluation indicator, the optimal formulation was verified by single factor experiment and orthogonal design; the general morphology, the particle size and distribution of the microcapsules were observed by light microscope (LM) and scanning electron microscope (SEM); the cumulative drug release rate of TF-PLGA microcapsules was detected by constant temperature commotion method in vitro and the release curves of the TF-PLGA were drawn. Results: The optimal prescription was as follows: The concentration of PLGA was 140 g. L-1 , oil phase volume was 1.4 mL, emulsifying speed was 900 r • min-1, emulsifying time was 5 min, the average EE was (83. 89±2. 30)% , and the average drug loading rate (DL) was (5. 90±0. 07)%. The LM and SEM resluts showed that the TF-PLGA microcapsules presented as round ball, the average particle size was (44. 34 ± 14. 68) fim, and the distribution was relatively narrow. The drug release in vitro results showed that the initial drug release rate (24 h) was about 40%, and the cumulative drug release rate was over 90% after 50 d. Conclusions The TF-PLGA sustained release microcapslue has better drug-loaded and sustained-release effects with simple preparation technique and better repeatability.
Liu H.,Weifang University |
Xiao J.,University of Coimbra |
Wang J.,Qingdao University of Science and Technology |
Jian F.,Qingdao University of Science and Technology |
And 2 more authors.
Advanced Materials Research | Year: 2012
Phosphorus vandium heteropolyacid sodium, H78N12NaO116P2V30, is prepared by the hydrothermal method. Cubic, Fm-3m. a =b = c = 20.022(2) Å α=β=γ= 90°. V=10680.0(17) Å3. Z=4. R1 =0.0388, wR2 =0.1200. T=298(2) K. The V atoms are linked with O atom bridge. The molecular structure is stabilized by the O-H.O and O-H.N intermolecular and intramolecular hydrogen-bond interaction. © (2012) Trans Tech Publications, Switzerland.
Han Y.,Jilin Business and Technology College |
Zhao S.-J.,JilinUniversity |
Proceedings 2011 International Conference on Human Health and Biomedical Engineering, HHBE 2011 | Year: 2011
In order to expand the development of health edible functions of Acanthopanax leaves, to meet people's demand for low care wine, Acanthopanax low alcohol wine was studied. Corn without Germ and Acanthopanax leaves are main materials, using double enzyme saccharification, low-temperature fermentation technology to produce low-alcohol wine. Saccharification process conditions was optimized by orthogonal experiment. The results showed that: Liquefaction time was 50 minutes, saccharification temperature was 55 □, glucoamylase dosage was 200U / g, pH5.0, saccharification time was 80 minutes, dosage of Acanthopanax leaves was 1.5 g / L. After Low-temperature fermentation, the alcohol concentration in an average was 4.5%. The appearance of wine was slightly yellow, clear and transparent. Because of a unique flavor and bitterness, no odor, and immune function, anti - fatigue, treatment of insomnia and so on, Acanthopanax low health wine has broad market and prospects. © 2011 IEEE.
Yu D.N.,JilinUniversity |
Shao C.M.,JilinUniversity |
Han L.,China First Automobile Works Group Corporation |
Zhang B.Y.,JilinUniversity |
Advanced Materials Research | Year: 2014
A trunk lid plate is taken as an example and Fiberglass-reinforced PET material is used to replace steel for weight-saving in this paper. Chooseinjection molding as plate's forming methodto find the optimal parameters in given range. To predict plastic parts' warpage under different injection parameters and find the optimal parameters, the method of Artificial Neural Network (ANN) is used. © (2014) Trans Tech Publications, Switzerland.
Li T.,University of Science and Technology Beijing |
Zou Y.,JilinUniversity |
Jiang S.,University of Science and Technology Beijing
Applied Mechanics and Materials | Year: 2012
A novel mesoporous composite water purification material was synthesized from natural zeolite to possess certain water purification properties as well as in proper particle size range for optimal fluid flow, particle retention and mass transfer in fixed bed applications. The meerschaum was used as the framework material in the experimental manufacturing procedure, polyethylene alcohol with carboxylic methylic cellulose was used as cohesive reagent, and H2O2 as bubble-forming agent. And the resulted granular product has good abrasion resistance ability with the measured impact breaking time reaching 12 hours. In addition, its decoloring rate is only 4. 4% lower and its CODcr removing is only 13. 2% lower than those of the original powder product, respectively. As a whole, the granulation potential and significantly expands the zeolite wastewater treatment application range with (only is not necessary) minor reduction of its treatment efficiency. © (2012) Trans Tech Publications, Switzerland.
PubMed | JilinUniversity
Type: Journal Article | Journal: IEEE transactions on bio-medical engineering | Year: 2011
Knee-joint kinematics analysis using an optimal sensor set and a reliable algorithm would be useful in the gait analysis. An original approach for ambulatory estimation of knee-joint angles in anatomical coordinate system is presented, which is composed of a physical-sensor-difference-based algorithm and virtual-sensor-difference-based algorithm. To test the approach, a wearable monitoring system composed of accelerometers and magnetometers was developed and evaluated on lower limb. The flexion/extension (f/e), abduction/adduction (a/a), and inversion/extension (i/e) rotation angles of the knee joint in the anatomical joint coordinate system were estimated. In this method, since there is no integration of angular acceleration or angular velocity, the result is not distorted by offset and drift. The three knee-joint angles within the anatomical coordinate system are independent of the orders, which must be considered when Euler angles are used. Besides, since there are no physical sensors implanted in the knee joint based on the virtual-sensor-difference-based algorithm, it is feasible to analyze knee-joint kinematics with less numbers and types of sensors than those mentioned in some others methods. Compared with results from the reference system, the developed wearable sensor system is available to do gait analysis with fewer sensors and high degree of accuracy.
PubMed | JilinUniversity
Type: Journal Article | Journal: Optics letters | Year: 2010
Electro-optic probing of electric fields has been considered as a promising approach for integrated circuit diagnosis. However, the method is subject to relatively weak voltage sensitivity. In this Letter, we solve the problems with electro-acoustic effect. In contrast to the general electro-optic effect, the light phase modulation induced by the acoustic effect is 2 orders of magnitude stronger at its resonant frequency, as we observed in a GaAs thin film probe. Furthermore, this what we believe to be a novel method shows a highly reproducible linearity between the detected signals and the input voltages, which facilitates the voltage calibration.