Jilin Yatai Group Co.
Jilin Yatai Group Co.
Yang S.,Chinese Institute of Scientific and Technical Information |
Wang Y.,Jilin Yatai Group Co. |
Chen H.,Chinese Institute of Scientific and Technical Information |
Zhou L.,Chinese Institute of Scientific and Technical Information |
And 2 more authors.
Gaojishu Tongxin/Chinese High Technology Letters | Year: 2016
An index system for patent value evaluation was established, and its 11 evaluation indexes were used to evaluate the values of the valid invention patents authorized from 2014 to 2105 in the machinery field of Jilin province, and the valuable patents with the possible market transformation prospects were obtained. The study interpreted the importance of the patent value evaluation, and pointed that value evaluation is a key link of patent industrialization; at present, few of patents performed the patent operation activities such as pledge, permission, transfer; there are still lots of the patents are not effectively transferred and used, thus they don't make a practical contribution to the economic development. The proposed patent value evaluation method is of significance to promoting the role of patents in economic development. © 2016, Inst. of Scientific and Technical Information of China. All right reserved.
Xie Y.-L.,Jilin Yatai Group Co. |
Zhou Q.-L.,Jilin Yatai Group Co. |
Wang W.-J.,Jilin Yatai Group Co. |
Yue Z.-Q.,Jilin Yatai Group Co. |
And 6 more authors.
Chinese Journal of Biologicals | Year: 2015
Objective: To optimize the condition for culture of influenza H5N1 virus in Vero cells. Methods: Influenza virus was cultured in various maintenance media [M199, M199 + epidermal growth factor (EGF), MEM, MEM + EGF and serum-free medium (SFM)] and M199 medium containing various concentrations of trypsin (3, 5 and 7 μg/ml) respectively, of which the culture supernatants were collected and determined for hemagglutinin (HA) titer to optimize the concentrations of maintenance medium and trypsin. Vero cells were infected with influenza H5N1 virus-infected cell supernatant before and after freezing and thawing and subcultured, of which the supernatant was collected and determined for HA titer to evaluate the effect of freezing and thawing on vims propagation. Vero cells were inoculated with influenza H5N1 virus at a MOI of 0.01 and cultured under the optimized condition, of which the culture supernatant was collected and determined for HA titer and infectious titer to evaluate the stability of H5N1 virus after continuous subculture in Vero cells. Results: When the SFM containing 5 μg/ml of trypsin was used, and the harvest was infected to Vero cells directly and subcultured for two passages before the maintenance medium was substituted with M199 medium, the HA titer of influenza H5N1 virus reached the maximum. Both the HA and infectious titers of H5N1 virus were stable after subculture in Vero cells under the optimized condition. Conclusion: Influenza H5N1 virus was subcultured continuously and stably in Vero cells under the optimized condition, which laid a foundation of establishment of working seed lot for the preparation of pandemic influenza vaccine.