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Liu B.,Jilin University | Dong Q.,Jilin University | Wang M.,Jilin University | Shi L.,Jilin University | And 9 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2010

Exenatide (synthetic exendin-4), a 39-amino acid peptide, was encapsulated in poly(DL-lactic-co-glycolic acid) (PLGA) microspheres as a sustained release delivery system for the therapy of type 2 diabetes mellitus. The microspheres were prepared by a double-emulsion solvent evaporation method and the particle size, surface morphology, drug encapsulation efficiency, in vitro release profiles and in vivo hypoglycemic activity were evaluated. The results indicated that the morphology of the exenatide PLGA microspheres presented as a spherical shape with smooth surface, and the particle sizes distributed from 5.8 to 13.6 μm. The drug encapsulation efficiency tested by micro-bicinchoninic acid (BCA) assay was influenced by certain parameters such as inner and outer aqueous phase volume, PLGA concentration in oil phase, polyvinyl alcohol (PVA) concentrations in outer aqueous phase. Moreover, in vitro release behaviors were also affected by some parameters such as polymer type, PLGA molecular, internal aqueous phase volume, PLGA concentration. The pharmacodynamics in streptozotocin (STZ)-induced diabetic mice suggested that, exenatide microspheres have a significant hypoglycemic activity within one month, and its controlling of plasma glucose was similar to that of exenatide solution injected twice daily with identical exenatide amount. In conclusion, this microsphere could be a well sustained delivery system for exenatide to treat type 2 diabetes mellitus. © 2010 Pharmaceutical Society of Japan.


Liu J.-Q.,Jilin Product Quality Supervision Inspection | Shi Y.-Y.,Jilin Product Quality Supervision Inspection | Liu J.-H.,Jilin Entry Exit Inspection and Quarant Bureau | Bing W.,Jilin Product Quality Supervision Inspection | And 4 more authors.
Journal of Jilin University Medicine Edition | Year: 2011

Objective: To establish a rapid, specific and sensitive method for the detection of Helicobacter pylori, and provide an effective detecting evidence for Helicobacter pylori. Methods: A pair of primers and probe corresponding to the urease gene for real time PCR were designed according to Primer Express 3.0 software, similar sequences were searched by Blast method, and the excellent primers and probe were selected. DNA from common bacterial pathogens such as Escherichia coli, Staphylococcus, histeria monocytogenes, Campylobacter and Salmonella in food was used for specific test; the counted Helicobacter pylori in bacterium suspension and the bacterium and milk mixture were serially diluted, the DNA was extracted for real time PCR amplification, the sensitivity of method was tested. The practicability of the method was demonstrated through the detection of the artificial contaminative samples by real time PCR. Results: The primers and probe according to urease gene could only amplify Helicobacter pylori DNA, but not other reference bacterium DNA. Helicobacter pylori from the artificial contaminative samples could be amplified by the method. It can detect 3 CFU · mL-1 of Helicobacter pylori. Its sensitivity was sufficient to detect 3 CFU · mL-1 of Helicobacter pylori. The method was enough rapid to finish detection in 4 d. Conclusion: Real time PCR can rapidly and accurately detect the Helicobacter pylori contamination in food with high specificity and sensitivity. © 1994-2012 China Academic Journal Electronic Publishing House. All rights reserved.

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