Yuan S.-X.,Jilin Institute of Animal Science and Veterinary Medicine |
Yao X.-H.,Jilin Institute of Animal Science and Veterinary Medicine |
Ren K.-Y.,Jilin Institute of Animal Science and Veterinary Medicine |
Li M.,Jilin Institute of Animal Science and Veterinary Medicine |
And 5 more authors.
Chinese Journal of Biologicals | Year: 2011
Objective: To prepare and verify the colloidal gold test strip for swine trichinelosis. Methods: The excretory-secretory (ES) antigen of swine trichina worm was labeled by immune colloidal gold technique and sprayed evenly onto glass wool. Rabbit anti-swine IgG was loaded onto AE98NC membrane as detection band, and anti-ES antibody as control band. NC printing membrane, glass wool loaded with colloidal gold-labeled ES antigen, water-absorbing paper and support plate were assembled into a test strip and verified for sensitivity, specificity and reproducibility, by which the detection result was compared with that by ELISA. Results: Thirty-eight serum samples positive for swine trichina worm were detected by the prepared test strip, and the results showed a sensitivity of 97.37%. Thirty-three serum samples positive for swine ascarides, 26 positive for pork tapeworm, 21 positive for Cysticerus cellulose, and 98 negative for swine trichina worm were detected by the test strip, and the result showed a specificity of 98. 88% and good reproducibility. No significant differences were observed in the sensitivity and specificity of the test strip and ELISA (P > 0.05). Conclusion: A colloidal gold test strip for swine trichinelosis was prepared, which was sensitive, specific, simple and rapid and suitable for the quarantine and epidemiological investigation of swine trichinellosis.