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Zhang X.,Northeastern University China | Ye S.,Jilin University | Zhang X.,Jilin Entry exit Inspection and Quarantine Bureau | Wu L.,Jilin Entry exit Inspection and Quarantine Bureau
Journal of Materials Chemistry C | Year: 2015

Optical properties of SiO2@Pd and SiO2@Pt core-shell nanoparticles (NPs) are investigated experimentally and theoretically, combined with a systematic comparison with those of traditionally investigated SiO2@Au NPs. Theoretical calculations show that both the plasmon hybridization effect and the retardation effect influence the spectral peak position of all three kinds of core-shell NPs. These two effects compete with each other. Plasmon hybridization is a dominant influence in the case of a thinner shell, whereas the retardation effect becomes more important when the shell becomes thicker. As a result, the dipolar plasmon band reveals an initial blue shift, and then red shift, with the increase of shell thickness. Furthermore, the influences of core size and shell material on the competition are discussed. Finally, the relative strengths of absorption and scattering associated with the LSPR of the three kinds of core-shell NPs are investigated. For SiO2@Pd and SiO2@Pt NPs, extinction is found to be dominated by absorption when shell thickness is less than ∼20 nm, and a crossover from absorption dominance to scattering dominance takes place with the further increase of shell thickness. In contrast, scattering is always the main decay process for SiO2@Au NPs, which contribute more than 90% intensity of the extinction despite the shell thickness. © The Royal Society of Chemistry 2015. Source

Liu J.-H.,Jilin Entry exit Inspection and Quarantine Bureau
Chinese Journal of Biologicals | Year: 2012

Objective: To analyze the residual host cell nucleic acid in commercial DNA polymerase products manufactured by various manufacturers. Methods: A Taqman probe-based real-time PCR method was developed by using the specific primers and probes designed according to E. coli 16S rRNA gene sequence, and used for analysis of residual E. coli DNA in recombinant products. Results: Residual E. coli DNAs were detected in all the DNA polymerase products manufactured by various manufacturers by the developed real-time PCR, of which the contents were different. Conclusion: When recombinant products especially those prepared with E. coli were tested by PCR, the effect of residual host cell nucleic acid in DNA polymerase on test result should be paid more attention. It suggested that the Ct value of real-time PCR for quantitative determination should be controlled to 30 or below so as to minimize the effect of background residue of DNA polymerase. Source

Zhang H.,Jilin University | Sun Y.,Jilin University | Wang J.,Jilin University | Zhang J.,Jilin University | And 2 more authors.
Biosensors and Bioelectronics | Year: 2012

A novel nanocomposite Fe 3O 4-Au nanorod (AuNR) was prepared and used as the substrate in the surface plasmon resonance (SPR) biosensor to detect goat IgM. Fe 3O 4-AuNR nanocomposites were synthesized by a method of seed-mediated growth, and further characterized by molecular absorption spectroscopy, transmission electronic microscopy (TEM), energy-dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS). The nanocomposites exhibit both magnetic property and exceptional optical property, which are beneficial to the antibody immobilization and the sensitivity of detection. The sensing membrane can be regenerated easily and the experimental procedure is simplified. Moreover, the Au nanorods show two plasmon resonance wavelengths defined as transverse mode and longitudinal mode, and the longitudinal plasmon wavelengths are more sensitive to the changes in the dielectric properties of the surroundings. Fe 3O 4-AuNR nanocomposites got a high sensitivity in detection of antibody-antigen immunoassay. In the optimal conditions, the biosensor based on Fe 3O 4-AuNR nanocomposites exhibits a satisfactory response to goat IgM in the concentration range of 0.15-40.00μgmL -1. However, the biosensor without Fe 3O 4-AuNR nanocomposites shows a response to goat IgM in the concentration range of 1.25-40.00μgmL -1. As a result, the sensitivity of the biosensor based on Fe 3O 4-AuNR nanocomposites is enhanced significantly. © 2012 Elsevier B.V. Source

Tong S.,Jilin University | Zhou X.,Jilin Entry exit Inspection and Quarantine Bureau | Zhou C.,Jilin University | Li Y.,Jilin University | And 3 more authors.
Analyst | Year: 2013

Graphene oxide (GO)/graphene (GN) nanosheets were coated onto the poly(glycidyl methacrylate-ethylene dimethacrylate) monolithic bed synthesized inside the capillary in order to prepare a promising polymer monolith microextraction (PMME) material (GO/GN@poly(GMA-EDMA)). Various techniques, including Fourier transform infrared spectroscopy, atomic force microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy, were employed to characterize the synthesized GO/GN@poly(GMA-EDMA) monoliths, confirming that GO/GN was effectively functionalized on the poly(GMA-EDMA) monolithic materials. Furthermore, a new method was developed for the analysis of sarcosine (identified as a potential prostate cancer biomarker) using PMME coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Under the preoptimized conditions, the monolithic column afforded satisfactory enhancement factor (32-fold) and low limits of detection (1.0 ng mL-1) were obtained. In comparison to several other commercialized solid phase extraction adsorbents, GN@poly(GMA-EDMA) monoliths exhibited excellent performance with recoveries of sarcosine approaching 93% with relative standard deviations less than 11.5%. © 2013 The Royal Society of Chemistry. Source

Qu X.-Y.,Jilin University | Hu T.-T.,Jilin Entry exit Inspection and Quarantine Bureau | Zhou W.,Jilin University
Brazilian Journal of Infectious Diseases | Year: 2015

Objective: The aim of this article is to compare the efficacy and safety of doripenem for bacterial infections. Methods: We included six randomized clinical trials identified from PubMed and Embase up to July 31, 2014. The included trials compared efficacy and safety of doripenem for complicated intra-abdominal infections, complicated urinary tract infection, nosocomial pneumonia, and acute biliary tract infection. The meta-analysis was carried on by the statistical software of Review Manager, version 5.2. Results: Compared with empirical antimicrobial agents on overall treatment efficacy, doripenem was associated with similar clinical and microbiological treatment success rates (for the clinical evaluable population, odds ratio [OR]. =. 1.26, 95% confidence interval [CI] 0.93-1.69, p=. 0.13; for clinical modified intent-to-treatment population, OR. =. 0.88, 95% CI 0.55-1.41, p=. 0.60; for microbiology evaluable population, OR. =. 1.16, 95% CI 0.90-1.50, p=. 0.26; for microbiological modified intent-to-treatment (m-mITT), OR. =. 0.98, 95% CI 0.81-1.20, p=. 0.87). We compared incidence of adverse events and all-cause mortality to analyze treatment safety. The outcomes suggested that doripenem was similar to comparators in terms of incidence of adverse events and all-cause mortality on modified intent-to-treatment population (for incidence of AEs, OR. =. 1.10, 95% CI 0.90-1.35, p=. 0.33; for all-cause mortality, OR. =. 1.08, 95% CI 0.77-1.51, p=. 0.67). In nosocomial pneumonia and ventilator-associated pneumonia treatment, doripenem was not inferior to other antibacterial agents in terms of efficacy and safety. Conclusion: From this meta-analysis, we can conclude that doripenem is as valuable and well-tolerated than empirical antimicrobial agents for complicated intra-abdominal infections, complicated urinary tract infection, acute biliary tract infection and nosocomial pneumonia treatment. © 2015 Elsevier Editora Ltda. Source

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