Jilin Entry exit Inspection and Quarantine Bureau

Changchun, China

Jilin Entry exit Inspection and Quarantine Bureau

Changchun, China
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Yue F.-G.,Jilin University | Dong L.,Jilin University | Hu T.-T.,Jilin Entry Exit Inspection and Quarantine Bureau | Hu T.-T.,Jilin University | Qu X.-Y.,Jilin University
Urology | Year: 2015

Objective To evaluate the efficacy of dapoxetine in the treatment of premature ejaculation (PE) with a meta-analysis method. Method We looked for randomized controlled trials (RCTs) from MEDLINE, EMBASE, Cochrane library, "International Standard Randomized Controlled Trial Number Register," and "ClinicalTrials.gov," which reported efficacy of dapoxetine for PE. Two reviewers searched and examined the RCTs independently. The meta-analysis was performed by RevMan5.0 software. Results We included 5 RCTs comparing dapoxetine with placebo. Dapoxetine was more effective than placebo for intravaginal ejaculatory latency time (weighted mean difference = 1.47; 95% confidence interval [CI] = 1.22-1.71; P <.00001). For the 4 patient-reported outcomes, dapoxetine was also more effective (for clinical global impression of change, odds ratio [OR] = 3.19; 95% CI, 2.47-4.11; P <.00001; for composite patient-reported outcomes criteria for clinical benefit, OR = 2.29; 95% CI, 1.74-3.00; P <.00001; for satisfaction with sexual intercourse, OR = 1.89; 95% CI, 1.68-2.12; P <.00001; for decrease in personal distress related to ejaculation, OR = 0.72; 95% CI, 0.57-0.90; P <.00001). Conclusion Dapoxetine is effective and well tolerated for either lifelong or acquired PE. But the long-term benefits and safety remain to be investigated. © 2015 Elsevier Inc.

Zhang X.,Northeastern University China | Ye S.,Jilin University | Zhang X.,Jilin Entry Exit Inspection and Quarantine Bureau | Wu L.,Jilin Entry Exit Inspection and Quarantine Bureau
Journal of Materials Chemistry C | Year: 2015

Optical properties of SiO2@Pd and SiO2@Pt core-shell nanoparticles (NPs) are investigated experimentally and theoretically, combined with a systematic comparison with those of traditionally investigated SiO2@Au NPs. Theoretical calculations show that both the plasmon hybridization effect and the retardation effect influence the spectral peak position of all three kinds of core-shell NPs. These two effects compete with each other. Plasmon hybridization is a dominant influence in the case of a thinner shell, whereas the retardation effect becomes more important when the shell becomes thicker. As a result, the dipolar plasmon band reveals an initial blue shift, and then red shift, with the increase of shell thickness. Furthermore, the influences of core size and shell material on the competition are discussed. Finally, the relative strengths of absorption and scattering associated with the LSPR of the three kinds of core-shell NPs are investigated. For SiO2@Pd and SiO2@Pt NPs, extinction is found to be dominated by absorption when shell thickness is less than ∼20 nm, and a crossover from absorption dominance to scattering dominance takes place with the further increase of shell thickness. In contrast, scattering is always the main decay process for SiO2@Au NPs, which contribute more than 90% intensity of the extinction despite the shell thickness. © The Royal Society of Chemistry 2015.

Lu C.,Jilin University | Wang M.,Jilin Entry Exit Inspection and Quarantine Bureau | Mub J.,Jilin Entry Exit Inspection and Quarantine Bureau | Han D.,Jilin Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
Food Chemistry | Year: 2013

A method for the simultaneous determination of eighteen steroid hormones, including 17a-ethinylestradiol, 17α-estradiol, 19-nortestosterone, estriol, testosterone, androsterone, 17β-estradiol, estrone, progesterone, 17α-hydroxyprogesterone, medroxyprogesterone, norethisterone acetate, testosterone 17- propionate, medroxyprogesterone-17-acetate, corticosterone, megestrol-17-acetate, chlromadinone-17- acetate and 17β-estradiol-benzoate, in antler velvet was developed by solid-phase extraction (SPE) and gas chromatography-tandem mass spectrometry (GC-MS/MS). Prior to the determination by GC-MS/MS, the analytes were derivatized with heptafluorobutyric acid anhydrides (HFBA). The recoveries were in the range of 62.13-104.00% for eighteen compounds at concentration levels (5-500 μg/L) and relative standard deviations (RSDs) were lower than 17.15%. The detection limits were from 0.2 to 22.3 μg/kg for the steroid hormones. The developed method was rapid, sensitive and successfully applied to the determination of steroid hormones in antler velvet samples collected from three countries. © 2013 Elsevier Ltd. All rights reserved.

Meng Q.-F.,Jilin Entry Exit Inspection and Quarantine Bureau | You H.-L.,Jilin University | Zhou N.,Qingdao University | Dong W.,Weihaiwei Peoples Hospital | And 2 more authors.
International Journal of Infectious Diseases | Year: 2015

Objective: Infection by the protozoan parasite Toxoplasma gondii is generally prevalent in animals and humans worldwide. However, little is known about T. gondii infection among children in China. Thus, the present study was conducted to detect the seroprevalence of T. gondii infection and estimate associated risk factors among children in eastern China. Methods: A cross-sectional study of 1500 children from three cities (Changchun, Qingdao, Weihai) was conducted between May 2013 and July 2014 to estimate the seroprevalence and potential risk factors associated with acquiring T. gondii infection in children in China. Demographic and blood samples were collected, and anti-. T. gondii IgG and IgM antibodies were measured by ELISA. Results: The mean age of the 1500 children participating in the study was 9.03 years (range 1-18). The overall seroprevalence of Toxoplasma infection was 15.13%, of which 13.13% were positive for only anti-. Toxoplasma IgG antibodies, 3.13% were positive for both anti-. Toxoplasma IgG and IgM antibodies and 2.00% were IgM positive and IgG negative. Moreover, raising cats at home (OR=1.94, 95% CI=1.288-2.912, P=0.002) and hand washing habits (OR=0.38, 95% CI=0.230-0.635, P<0.001) were estimated to be risk and protective factors associated with Toxoplasma seroprevalence, respectively. Conclusions: The present study revealed for the first time that children's infection with T. gondii is common in eastern China. The present data indicate a need to implement methods of prevention and control measures against Toxoplasma infection in China. © 2014 The Authors.

Liu J.-H.,Jilin Entry Exit Inspection and Quarantine Bureau
Chinese Journal of Biologicals | Year: 2012

Objective: To analyze the residual host cell nucleic acid in commercial DNA polymerase products manufactured by various manufacturers. Methods: A Taqman probe-based real-time PCR method was developed by using the specific primers and probes designed according to E. coli 16S rRNA gene sequence, and used for analysis of residual E. coli DNA in recombinant products. Results: Residual E. coli DNAs were detected in all the DNA polymerase products manufactured by various manufacturers by the developed real-time PCR, of which the contents were different. Conclusion: When recombinant products especially those prepared with E. coli were tested by PCR, the effect of residual host cell nucleic acid in DNA polymerase on test result should be paid more attention. It suggested that the Ct value of real-time PCR for quantitative determination should be controlled to 30 or below so as to minimize the effect of background residue of DNA polymerase.

Zhang H.,Jilin University | Sun Y.,Jilin University | Wang J.,Jilin University | Zhang J.,Jilin University | And 2 more authors.
Biosensors and Bioelectronics | Year: 2012

A novel nanocomposite Fe 3O 4-Au nanorod (AuNR) was prepared and used as the substrate in the surface plasmon resonance (SPR) biosensor to detect goat IgM. Fe 3O 4-AuNR nanocomposites were synthesized by a method of seed-mediated growth, and further characterized by molecular absorption spectroscopy, transmission electronic microscopy (TEM), energy-dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS). The nanocomposites exhibit both magnetic property and exceptional optical property, which are beneficial to the antibody immobilization and the sensitivity of detection. The sensing membrane can be regenerated easily and the experimental procedure is simplified. Moreover, the Au nanorods show two plasmon resonance wavelengths defined as transverse mode and longitudinal mode, and the longitudinal plasmon wavelengths are more sensitive to the changes in the dielectric properties of the surroundings. Fe 3O 4-AuNR nanocomposites got a high sensitivity in detection of antibody-antigen immunoassay. In the optimal conditions, the biosensor based on Fe 3O 4-AuNR nanocomposites exhibits a satisfactory response to goat IgM in the concentration range of 0.15-40.00μgmL -1. However, the biosensor without Fe 3O 4-AuNR nanocomposites shows a response to goat IgM in the concentration range of 1.25-40.00μgmL -1. As a result, the sensitivity of the biosensor based on Fe 3O 4-AuNR nanocomposites is enhanced significantly. © 2012 Elsevier B.V.

Tong S.,Jilin University | Zhou X.,Jilin Entry Exit Inspection and Quarantine Bureau | Zhou C.,Jilin University | Li Y.,Jilin University | And 3 more authors.
Analyst | Year: 2013

Graphene oxide (GO)/graphene (GN) nanosheets were coated onto the poly(glycidyl methacrylate-ethylene dimethacrylate) monolithic bed synthesized inside the capillary in order to prepare a promising polymer monolith microextraction (PMME) material (GO/GN@poly(GMA-EDMA)). Various techniques, including Fourier transform infrared spectroscopy, atomic force microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy, were employed to characterize the synthesized GO/GN@poly(GMA-EDMA) monoliths, confirming that GO/GN was effectively functionalized on the poly(GMA-EDMA) monolithic materials. Furthermore, a new method was developed for the analysis of sarcosine (identified as a potential prostate cancer biomarker) using PMME coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Under the preoptimized conditions, the monolithic column afforded satisfactory enhancement factor (32-fold) and low limits of detection (1.0 ng mL-1) were obtained. In comparison to several other commercialized solid phase extraction adsorbents, GN@poly(GMA-EDMA) monoliths exhibited excellent performance with recoveries of sarcosine approaching 93% with relative standard deviations less than 11.5%. © 2013 The Royal Society of Chemistry.

Qu X.-Y.,Jilin University | Hu T.-T.,Jilin Entry Exit Inspection and Quarantine Bureau | Zhou W.,Jilin University
Brazilian Journal of Infectious Diseases | Year: 2015

Objective: The aim of this article is to compare the efficacy and safety of doripenem for bacterial infections. Methods: We included six randomized clinical trials identified from PubMed and Embase up to July 31, 2014. The included trials compared efficacy and safety of doripenem for complicated intra-abdominal infections, complicated urinary tract infection, nosocomial pneumonia, and acute biliary tract infection. The meta-analysis was carried on by the statistical software of Review Manager, version 5.2. Results: Compared with empirical antimicrobial agents on overall treatment efficacy, doripenem was associated with similar clinical and microbiological treatment success rates (for the clinical evaluable population, odds ratio [OR]. =. 1.26, 95% confidence interval [CI] 0.93-1.69, p=. 0.13; for clinical modified intent-to-treatment population, OR. =. 0.88, 95% CI 0.55-1.41, p=. 0.60; for microbiology evaluable population, OR. =. 1.16, 95% CI 0.90-1.50, p=. 0.26; for microbiological modified intent-to-treatment (m-mITT), OR. =. 0.98, 95% CI 0.81-1.20, p=. 0.87). We compared incidence of adverse events and all-cause mortality to analyze treatment safety. The outcomes suggested that doripenem was similar to comparators in terms of incidence of adverse events and all-cause mortality on modified intent-to-treatment population (for incidence of AEs, OR. =. 1.10, 95% CI 0.90-1.35, p=. 0.33; for all-cause mortality, OR. =. 1.08, 95% CI 0.77-1.51, p=. 0.67). In nosocomial pneumonia and ventilator-associated pneumonia treatment, doripenem was not inferior to other antibacterial agents in terms of efficacy and safety. Conclusion: From this meta-analysis, we can conclude that doripenem is as valuable and well-tolerated than empirical antimicrobial agents for complicated intra-abdominal infections, complicated urinary tract infection, acute biliary tract infection and nosocomial pneumonia treatment. © 2015 Elsevier Editora Ltda.

Pan D.,Northeast Normal University | Ma S.,Jilin Entry Exit Inspection and Quarantine Bureau | Bo X.,Northeast Normal University | Guo L.,Northeast Normal University
Microchimica Acta | Year: 2011

Ordered mesoporous carbon (OMC) was synthesized and used to modify the surface of a glassy carbon (GC) electrode. Due to the unique properties of OMC, a decrease in the overvoltage of the reduction potential of methyl parathion (MP) (to ca. 219 mV) and a 76-fold increase in the peak current are observed (compared with a bare GC electrode). The absorption capacity of the surface of the electrode for MP was determined by chronocoulometry. The results show that the γ value of the modified electrode (2.34 × 10-9 mol cm-2) is 9.5 times as large as that of the GC electrode (2.47 × 10-10 mol cm-2). The new electrode exhibits synergistic electrocatalytic and accumulative effects on MP. MP can be determined by linear sweep voltammetry (LSV) which displays a linear relationship between peak current and MP concentration in the range from 0. 09 to 61 μM, with a detection limit as low as 7.6 nM (at an S/N of 3) and after an accumulation at 0 V for 5 min. The electrode was successfully applied to the determination of MP in spiked lake water samples. © 2011 Springer-Verlag.

Li W.,Jilin University | Zhou X.,Jilin Entry Exit Inspection and Quarantine Bureau | Ye J.,Jilin University | Jia Q.,Jilin University
Journal of Separation Science | Year: 2013

Monolithic materials were synthesized in capillaries by in situ polymerization with N-isopropylacrylamide, glycidyl methacrylate, and ethylene dimethacrylate as the monomers, and methanol and PEG as the porogens. With γ-alumina nanoparticles attached to the surface of the porous monolithic column via epoxide groups, a novel polymer monolith microextraction (PMME) material was prepared with a good mechanical stability and a high extraction capacity. SEM and X-ray photoelectron spectroscopy were employed to characterize the modified monolithic column, demonstrating that γ-alumina nanoparticles were effectively functionalized onto the monolithic column. In addition, a new method was developed for the analysis of Sudan I-IV dyes using PMME coupled with HPLC. In order to obtain the optimum extraction efficiency, the PMME conditions including desorption solvent type, sample pH, sample volume, sample flow rate, and eluent flow rate were investigated. Under the optimum conditions, we obtained acceptable linearities, low LODs, and good intra- and interday RDSs. When applied to the determination of Sudan I-IV dyes in red wine samples, satisfactory recoveries were obtained in the range of 84.0-115.9%. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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