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Ding Y.,Zhejiang University City College | Lu L.,Zhejiang University City College | Zhang R.,Jiaxing Second Hospital | Zeng L.,Zhejiang University City College | And 2 more authors.
PLoS ONE | Year: 2015

Nischarin is a cytoplasmic protein expressed in various organs that plays an inhibitory role in cell migration and invasion and the carcinogenesis of breast cancer cells. We previously reported that Nischarin is highly expressed in neuronal cell lines and is differentially expressed in the brain tissue of adult rats. However, the physiological function of Nischarin in neural cells remains unknown. Here, we show that Nischarin is expressed in rat primary cortical neurons but not in astrocytes. Nischarin is localized around the nucleus and dendrites. Using shRNA to knockdown the expression of endogenous Nischarin significantly increases the percentage of neurite-bearing cells, remarkably increases neurite length, and accelerates neurite extension in neuronal cells. Silencing Nischarin expression also promotes dendrite elongation in rat cortical neurons where Nischarin interacts with p21-activated kinase 1/2 (PAK1/2) and negatively regulates phosphorylation of both PAK1 and PAK2. The stimulation of neurite growth observed in cells with decreased levels of Nischarin is partially abolished by IPA3-mediated inhibition of PAK1 activity. Our findings indicate that endogenous Nischarin inhibits neurite outgrowth by blocking PAK1 activation in neurons. © 2015 Ding et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Li B.,Jiaxing Second Hospital | Wang L.,Shanghai JiaoTong University | Lu Q.,Jiaxing Second Hospital | Da W.,Shanghai JiaoTong University
Irish Journal of Medical Science | Year: 2016

Aim: Nuclear factor-erythroid 2-related factor-2 (Nrf2) acts as a defense system in the development of nonalcoholic steatohepatitis (NASH). Curcumin is a phenolic compound with lipid regulatory, anti-oxidative, anti-inflammatory and anti-tumorigenic properties that is beneficial in defending against NASH and was recently proved to be an Nrf2 activator. The aim of this study was to evaluate whether Nrf2 activation could be involved in NASH mitigation by curcumin. Methods: Hepatic, metabolic, and inflammatory parameters, along with hepatic Nrf2 protein expression were explored in adult Sprague–Dawley rats developing high-fat-diet-induced NASH and submitted to curcumin gavage for 6 weeks. Results: Curcumin administration led to lower degrees of hepatic steatosis and inflammation; lower levels of serum aminotransferases, lipids, and homeostasis model assessment of insulin resistance; and lower serum and hepatic contents of tumor necrosis factor-α (TNF-α), interleukin-6, and malondialdehyde. In contrast, higher hepatic contents of glutathione, heme oxygenase-1 and superoxide dismutase were observed in rats with curcumin. Moreover, Nrf2 expression in liver cell nuclei was significantly higher in rats with curcumin. Conclusions: Curcumin can prevent and ameliorate NASH via lipid reduction, improve insulin resistance, improve anti-inflammatory, and have antioxidant effects, possibly related to its activation of Nrf2. © 2014, Royal Academy of Medicine in Ireland.


PubMed | Shanghai JiaoTong University and Jiaxing Second Hospital
Type: Journal Article | Journal: Irish journal of medical science | Year: 2016

Nuclear factor-erythroid 2-related factor-2 (Nrf2) acts as a defense system in the development of nonalcoholic steatohepatitis (NASH). Curcumin is a phenolic compound with lipid regulatory, anti-oxidative, anti-inflammatory and anti-tumorigenic properties that is beneficial in defending against NASH and was recently proved to be an Nrf2 activator. The aim of this study was to evaluate whether Nrf2 activation could be involved in NASH mitigation by curcumin.Hepatic, metabolic, and inflammatory parameters, along with hepatic Nrf2 protein expression were explored in adult Sprague-Dawley rats developing high-fat-diet-induced NASH and submitted to curcumin gavage for 6 weeks.Curcumin administration led to lower degrees of hepatic steatosis and inflammation; lower levels of serum aminotransferases, lipids, and homeostasis model assessment of insulin resistance; and lower serum and hepatic contents of tumor necrosis factor- (TNF-), interleukin-6, and malondialdehyde. In contrast, higher hepatic contents of glutathione, heme oxygenase-1 and superoxide dismutase were observed in rats with curcumin. Moreover, Nrf2 expression in liver cell nuclei was significantly higher in rats with curcumin.Curcumin can prevent and ameliorate NASH via lipid reduction, improve insulin resistance, improve anti-inflammatory, and have antioxidant effects, possibly related to its activation of Nrf2.


Hou M.-H.,Tianjin 4th Central Hospital | Chen M.,Tianjin Medical University | Li L.,Beijing Space Center Hospital | Zhang S.-S.,Tianjin Stomatological Hospital | And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: In root canal preparation, the clearance effect of sodium hypochlorite for root canal irrigation on the smear layer is associated with the contact time and contact area of irrigation fluid, the amount of irrigation fluid, irrigation fluid temperature, irrigation fluid concentration, flushing methods and so on. OBJECTIVE: To investigate the clearance effect of 3% sodium hypochlorite on the smear layer of root canal under different flushing methods, flushing temperature and flushing time. METHODS: Totally 180 isolated front teeth with single root were collected and root canal preparation was conducted using motorized ProTaper. The root canal was flushed with 3% sodium hypochlorite. According to different flushing methods, these root canals were randomly and evenly divided into root canal active flushing group, passive ultrasound swished group and side opening flushing needle group. According to the flushing temperature and the flushing time after replacing a file, each group was randomly divided into six subgroups: 20 °C 30 seconds group, 20 °C 60 seconds group, 37 °C 30 seconds group, 37 °C 60 seconds, 60 °C 30 seconds group and 60 °C 60 seconds group. The clearance effect on the smear layer of root canal was observed using scanning electron microscope. RESULTS AND CONCLUSION: The general clearance effect on the smear layer in the root canal active flushing group was better than that in the passive ultrasound swished group and side opening flush acupuncture group (P < 0. 05). At the same time, the clearance effect on the smear layer in the 60 °C group was better that that in the 37 °C group and 20 °C group (P < 0. 05). At a certain temperature, the clearance effect on the smear layer in 60 seconds group was better than that in the 30 seconds group (P < 0. 05). The clearance effect on the smear layer in the 1/3 root cap zone was better than that in the 1/3 root and 1/3 apical root zone (P < 0. 05). These results show that the best clearance effect on the smear layer can be achieved as follows: 3% sodium hypochlorite irrigation at 60°C for 60 seconds. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


You D.-J.,Chinese People's Liberation Army | Chang D.,Dalian Medical University | Zhang S.-L.,Dalian Medical University | Yang D.-H.,Jilin University | And 7 more authors.
Chinese Medical Journal | Year: 2012

Background Ablation of complex fractionated atrial electrograms (CFAE) is an important adjunctive therapy in atrial fibrillation (AF). The present study was to elucidate the substrate underlying CFAE. Methods Nine adult mongrel dogs were involved in the present study. AF was induced through rapid atrial pacing with vagosympathetic nerve stimulation. CFAE was recorded during AF. Ablation was performed at CFAE sites. Based on the location of the ablation scar, the atrial specimens were divided into CFAE and non-CFAE sites. Serial sections of the atrium were stained respectively with hematoxylin-eosin (HE) and the general neural marker protein gene product 9.5 (PGP9.5). We compared the characteristics of the myocardium and the ganglionated plexus (GPs) distribution between the CFAE and non-CFAE sites. Results The myocardium of non-CFAE sites was well-organized with little intercellular substance. However, the myocardium in the CFAE site was disorganized with more interstitial tissue ((61.7±24.3)% vs. (34.1±9.2)%, P<0.01). GPs in the CFAE site were more abundant than in non-CFAE sites ((34.45±37.46) bundles/cm 2 vs. (6.73± 8.22) bundles/cm2, P<0.01). Conclusion The heterogeneity of the myocardium and GPs distribution may account for the substrate of CFAE and serve as a potential target of ablation.


Zhang H.Y.,Jiaxing Second Hospital | Chai D.Z.,Jiaxing Second Hospital | Fu W.G.,Jiaxing Second Hospital | Wang Y.Q.,Jiaxing Second Hospital | And 5 more authors.
EJVES Extra | Year: 2012

Introduction: Internal jugular vein aneurysms are very uncommon. Indications for surgery include the compression of adjacent structures and the risk of pulmonary embolism associated with thrombus within the aneurysm. Report: A 78-year-old woman was hospitalised with a large internal jugular vein aneurysm filled with thrombus. A bare metal stent was deployed from the left subclavian vein to the left brachiocephalic vein and served as a protection device during the open surgical resection of the aneurysm. At 28-month follow-up no serious complications had occurred. Discussion: This case report introduces a novel method to prevent pulmonary embolism during treatment of large partially thrombosed jugular vein aneurysms. © 2012 European Society for Vascular Surgery.


Xue M.-F.,Jiaxing Second Hospital | Gong S.-L.,Jiaxing Second Hospital | Dai J.-P.,Jiaxing Second Hospital | Chen G.,Jiaxing Second Hospital | Hu J.-Y.,Jiaxing Second Hospital
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2015

Objective: To explore the establishment of a fibrosis model in vitro by inducing rat myoblast C2C12 by transforming growth factor-β (TGF-β), observe the effects of variations of the miRNA138 level on cell fibrosis indexes, and analyze whether the effects of miRNA138 on cell fibrosis was achieved by its target gene Smad4. Methods: The miRNA138-mimics, miRNA138-inhibitor, and miRNA138-NC were designed, synthesized and transfected into C2C12 cells by lipofectminae. Transfected cells were then induced by TGF-β1 of 10 ng/mL for 48 h. The expression of miRNA138 was detected by Real-time PCR. The variations of expressions of fibrosis related proteins Smad4, vimentin, α-SMA, and collagen I were detected by the Western blotting to verify whether the fibrosis model had been successfully established and analyze the effects of variations of the miRNA 138 expression on cell fibrosis indexes. The effects of variations of the miRNA 138 expression on the proliferation activity of C2C12 during the course of fibrosis were measured by the CCK-8. Bioinformatic software was used to analyze the theoretical binding site of miRNA138 and Smad4, which was verified by the luciferase reporter gene test. The Smad4 gene expression vector was constructed and co-transfected into C2C12 cells with miRNA-mimics. Transfected cells were induced by TGF-β1 for fibrosis. The variations of fibrosis related proteins were detected by the Western blotting and the effects of expression of exogenous Smad4 on the inhibition of cell fibrosis by miRNA138 were analyzed. Results: The expression of miRNA138 significantly increased after C2C12 cells were transfected by miRNA138-mimics and decreased after C2C12 cells were transfected by miRNA138-inhibitor. The expression of miRNA138 of the control group had no significant changes. Results: of protein detection showed that expressions of Smad4, vimentin, α-SMA, and collagen I were significantly increased and the cell proliferation activity in logarithmic phase was enhanced after C2C12 cells were induced by TGF-β1 for 48 h, which indicated that the cell fibrosis model was successfully established. The over-expression of miRNA138 significantly inhibited the fibrosis of C2C12 cells. The data of luciferase activity detections showed that the predicted binding site of miRNA138 and Smad4 existed. High expression of exogenous Smad4 in C2C12 cells significantly impaired the inhibition of cell fibrosis by miRNA138-mimics.Conclusion: MiRNA138 can inhibit the fibrosis of C2C12 cells induced by TGF-β1 via suppressing the expression of its target protein Smad4. ©, 2015, Editorial Department of Journal of Shanghai Second Medical University. All right reserved.


Teng Y.Q.,Jiaxing Second Hospital | Yao J.X.,Jiaxing Second Hospital | Zhu W.,Jiaxing Second Hospital | Yan X.,Jiaxing Second Hospital | And 2 more authors.
Hong Kong Journal of Paediatrics | Year: 2011

Objectives: To analyse the clinical manifestations of neonates suffering from cow's milk allergy (CMA), and to explore the treatment of CMA in neonates, as well as to conduct a one-year follow-up study. Study design: To conduct a clinical chart review of a small cohort of neonates of confirmed cow's milk allergy. Results: Eleven neonates were diagnosed as suffering from CMA from May 2008 to May 2010, all of which also had digestive tract symptoms. Five were suffering from eczema at the same time. Five had positive sIgE. The other six got negative sIgE and diagnosed as having CMA when using milk to conduct food-avoiding challenge test. In the following one year, nine got tolerance to milk. Conclusions: The clinical manifestations of neonates suffering from CMA were diversified; the positive rate of sIgE is not high; food-elimination challenge test could be an alternative for diagnosing food allergy. Significant outcome can be achieved when applying food-avoiding treatments to milk-fed infants. Within one year, 9/11 (82%) of the infants suffering from CMA got tolerance to cow's milk.


Wang K.,Dalian Medical University | Chang D.,Dalian Medical University | Chu Z.,Jiaxing Second Hospital | Yang Y.,Dalian Medical University | And 7 more authors.
The Scientific World Journal | Year: 2013

Backgrounds. Segmental and circumferential pulmonary vein isolations (SPVI and CPVI) have been demonstrated to be effective therapies for paroxysmal atrial fibrillation (PAF). PVI is well established as the endpoint of different ablation techniques, whereas it may not completely account for the long-term success. Methods. 181 drug-refractory symptomatic PAF patients were referred for segmental or circumferential PVI (SPVI = 67; CPVI = 114). Heart rate variability (HRV) was assessed before and after the final ablation. Results. After following up for 62.23±12.75 months, patients underwent 1.41±0.68 procedures in average, and the success rates in SPVI and CPVI groups were comparable. 119 patients were free from AF recurrence (SPVI-S, n=43; CPVI-S, n=76). 56 patients had recurrent episodes (SPVI-R, n=21; CPVI-R, n=35). Either ablation technique decreased HRV significantly. Postablation SDNN and rMSSD were significantly lower in SPVI-S and CPVI-S subgroups than in SPVI-R and CPVI-R subgroups (SPVI-S versus SPVI-R: SDNN 91.8±32.6 versus 111.5±36.2 ms, rMSSD 47.4±32.3 versus 55.2±35.2 ms; CPVI-S versus CPVI-R: SDNN 83.0±35.6 versus 101.0±40.7 ms, rMSSD 41.1±22.9 versus 59.2±44.8 ms; all P<0.05). Attenuation of SDNN and rMSSD remained for 12 months in SPVI-S and CPVI-S subgroups, whereas it recovered earlier in SPVI-R and CPVI-R subgroups. Multivariate logistic regression analysis identified SDNN as the only predictor of long-term success. Conclusions. Beyond PVI, denervation may be a common mechanism underlying different ablation strategies for PAF. © 2013 Kejing Wang et al.


Chen B.,Jiaxing Second Hospital | Fan S.,Zhejiang University | Zhao F.,Zhejiang University
Indian Journal of Orthopaedics | Year: 2014

Background: Intravertebral cleft is a structural change in osteoporotic vertebral compression fractures (OVCF), which is the manifestation of ischemic vertebral osteonecrosis complicated with fracture nonunion and pseudoarthrosis and appears in the late stage of OVCF. Despite numerous studies on OVCF, few aim to evaluate the clinicoradiological characteristics and clinical significance of intravertebral cleft in OVCF. This study investigates clinicoradiological characteristics of intravertebral cleft in OVCF and the effect on the efficacy of percutaneous balloon kyphoplasty (PKP). Materials and Methods: PKP was performed on 139 OVCF patients without intravertebral cleft (group A) and 44 OVCF patients with intravertebral cleft (group B). The frequency distribution of the affected vertebral body, bone cement infusion volume, imaging manifestation, leakage rate and type, preoperative and postoperative height of the affected vertebral body, visual analog scale (VAS) and Oswestry disability index (ODI) score were evaluated. Results: Significant differences were found in the frequency distribution of the affected vertebral body and bone cement leakage type between the two groups (P < 0.05). However, differences in bone cement infusion volume and leakage rate (P > 0.05) were not detected. In both groups, the postoperative height of the affected vertebral body was significantly improved (P < 0.05). The restoration of vertebral body height in group B was more evident than that in group A (P < 0.05). The preoperative VAS and ODI scores in group B were significantly higher than those in group A (P < 0.05). After surgical treatment, pain relief and daily activity function in both groups were significantly improved (P < 0.05), and no significant difference in postoperative scores was detected between the two groups (P > 0.05). Conclusion: Intravertebral cleft exhibits specific clinical and imaging as well as bone cement formation characteristics. PKP can effectively restore the affected vertebral body height, alleviate pain, and improve daily activity function of patients.

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