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Han Z.X.,Huaihai Institute of Technology | Han Z.X.,Chongqing Key Laboratory of Micro Nano Materials Engineering and Technology | Han Z.X.,Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection | Wu D.-D.,Huaihai Institute of Technology | And 3 more authors.
Synthesis and Reactivity in Inorganic, Metal-Organic and Nano-Metal Chemistry | Year: 2014

The anthropogenic release of carbon dioxide into the atmosphere leads to an increase in the CO2 partial pressure in the ocean, which may reach 950 ?atm by the end of the 21st century. An experiment was performed to test the effects of increased sea water concentrations of CO2 combined with heavy metals on mortality rates, metallothionein, immune funtion, and uptake kinetics of the bivalve Mytilus edulis L. As a result, the mortality rates of Mytilus edulis L fluctuated with pH variety in all heavy metals (Cd, Pb, Cu) test seawater treatments compared to control group. It was demonstrated that in vivo Cd-, Pb-, or Cu pretreatment under acidifications can alleviate metallothionein by in vitro Cd, Pb, and Cu in soft tissues and suggested that acidification can aggravate heavy metals pollution and toxicity for marine organisms. Moreover, the fraction of eosinophilic cells increased over the 21 days period for Cd exposure, then decreased with 28 and 35 days incubation, the fraction of eosinophilic cells increased similar with Cd exposure and pH 8.2. The fraction of eosinophilic cells significantly increased from 32% to 53% of the hemocyte population over the 35-day period for Pb exposure and pH8.2, the fraction of eosinophilic cells increased from 35% to 58% of the hemocyte population over the 35-day period for Pb exposure and pH 6.2. Phagocytosis levels declined with increasing exposure time for Cd alone and phagocytosis levels declined with decreasing pH (from 8.2 to 6.2) with Cd exposure on 35 days. Mytilus edulis L in Pb with reduced pH (6.2) treatments also decreased their phagocytosis levels on 35 days. Further, phagocytosis decreased bigger than that of Pb with pH 8.2. At steady state equilibrium, CF of 109Cd was significantly higher at pH 6.2 and pH 7.7 than at pH 8.2, the accumulation of 203Pb was significantly higher at the lowest pH 6.2 level than at the two exposure levels (pH 7.7 and pH 8.2). Similarly, the lower the seawater pH 6.2, the more 63Cu was accumulated in the hemolymph of Mytilus edulis L. The results indicate that future reductions in pH caused by increased CO2 concentrations in the sea may have an impact on Mytilus edulis L combined with heavy metals pollution. Copyright © Taylor & Francis Group, LLC. Source


Han Z.,Huaihai Institute of Technology | Zhangmeng,Huaihai Institute of Technology | Lv C.-X.,Jiaonan Agricultural Bureau
Advanced Materials Research | Year: 2012

Little is known about the potential behavior and ecotoxicity of nanoparticles to aquatic organisms. To carefully address this issue, we conducted a comprehensive toxicity assessment, including NiO nanoparticles accumulation and toxicity tests as well as Lipid peroxidation (LPO), MDA content, mitochondrial depolarization and Reactive oxygen radical (ROS) analysis using Gracilaria lemaneiformis as a model organism. Our results demonstrated that the concentration of NiO Nanoparticles accumulated in Gracilaria lemaneiformis increased with the extended incubation time and a considerable amount of NiO Nanoparticles were still present in the bodies of Gracilaria lemaneiformis. Exposure to NiO nanoparticles led to significant reductions of cell viability between 20 and 60% relative to controls. Elevated lipid peroxidation in Gracilaria lemaneiformis exposed to NiO nanoparticles compared to control and induced increases in MDA levels with incubation time. Significantly enhanced percent of mitochondrial depolarization in nanometer size compared to controls and NiO nanoparticles significantly elevated ROS levels more than three fold at the highest concentration. Taken together, these findings indicate that NiO nanoparticles exposure, especially for long periods of time, may exert negative impact on population of aquatic organisms and on food web dynamics in aquatic systems. © (2012) Trans Tech Publications, Switzerland. Source


Han Z.,Huaihai Institute of Technology | Han G.,Huaihai Institute of Technology | Zhangmeng,Huaihai Institute of Technology | Lv C.-X.,Jiaonan Agricultural Bureau
Advanced Materials Research | Year: 2012

This study was aimed at obtaining detailed information about the interaction of NaCl salinity and elevated atmospheric CO 2 concentration in the halophyte S.salsa, which was irrigated with five different salinity levels under ambient and elevated (530 ppm) CO 2. The results show that total soluble sugar concentration was significantly increased by salt-treatments in both leaves and roots, and that the most progressive sugar increments were observed in leaves and roots of S. salsa under the elevated CO 2. The Na + concentration in the leaves and roots increased with the increased NaCl concentration, the K + accumulation gradually decreaed by increasing salinity levels in leaves and roots. Proline increased in response to salt stress along with incremental NaCl concentration. The GB concentrations of leaves were significantly raised as NaCl levels increased. Electrolyte leakage increased in the leaves of S. salsa grown under NaCl stress. ψs of leaves and roots decreased as the NaCl concentration increase, and that elevated CO 2 both had markedly greater effects on ψs of leaves and roots. The osmotic adjustment values ascended with elevated CO 2 concentration in both leaves and roots. © (2012) Trans Tech Publications, Switzerland. Source


Han Z.,Huaihai Institute of Technology | Lv C.,Jiaonan Agricultural Bureau | Zheng Z.,Animal Quarantine Section
Chinese Journal of Geochemistry | Year: 2010

In this study the effects of polybrominated diphenyl ether compounds (PBDEs), BDE-47 and BDE-99 on carassius aurats were discussed. The reproductive capacity viability and proliferation percent decreased with increasing BDE-47 and BDE-99 concentrations (p<0.05), presenting a dose-dependent manner. Physiological biomarkers such as cholesterol (CHL), acetylcholinesterase (AChE), ethoxyresorufin-O-deethylase (EROD) and vitamin were studied to reveal the toxicological effects of BDE-47 and BDE-99 exposure. CHL levels generally decrease with increasing BDE-47 exposure. However, there were no significant differences between the samples and the control. As to BDE-99, the CHL level decreased as compared with the control level when the BDE-99 concentrations reached 20 μg/L (p<0.05). The degree of inhibition of the AChE increased with the increase of DE-47and BDE-99 concentrations. The EROD activity during 72 hours' exposure significantly increased to 20 μg/L for BDE-47 (p<0.05), however, EROD activity was not significantly different at different concentrations of BDE-99 (p<0.05). Carassius aurats hepatocytes reacted to the BDE-47 exposure with increasing EROD activities in the dose dependent manner. Although at lower concentrations, the induced effects of BDE-47 and BDE-99 were not significantly different, though BDE-47 showed strong induced effects on the EROD activity with increasing concentrations in carassius aurats hepatocytes. In contrast, BDE-99 did not influence EROD activity consistently. The VA level of carassius aurats was significantly different from that of the controls for BDE-99 (p<0.001), as far as the VA level was significantly lower of carassius aurats than the controls for BDE-47 (p<0.001). CHL, AChE, EROD, and VA levels in carassius aurats were differently affected by BDE-47 and BDE-99 exposures, therefore, they would be good indicators for exposure. © 2010 Science Press, Institute of Geochemistry, CAS and Springer Berlin Heidelberg. Source


Han Z.,Huaihai Institute of Technology | He G.,Huaihai Institute of Technology | Wang J.,Nanjing University | Lu C.,Jiaonan Agricultural Bureau
Chinese Journal of Geochemistry | Year: 2011

In this study, the joint effects of Cd(II), Pb(II) and atrazine (ATR) on Solidago Canadensis L. were investigated. The results showed that soil containing Cd, Pb and ATR could inhibit root elongation of Solidago Canadensis L., and that there was a positive linear relationship between the inhibitory rate of root elongation and the concentrations of Pb(II). The mixture of Cd(II) and Pb(II)-ATR in soil showed a significant adverse effect on root and shoot biomass of Solidago Canadensis L. Acetylsalicylic acid (ASA) contents increased slightly at the lower concentrations of Cd(II)-ATR, then decreased when Cd(II)-ATR concentrations were higher than those of 1. 0 TUmix(Cd+ATR). The toxic effect of Pb(II)-ATR on the ASA contents of Solidago Canadensis L. was greater than that of Cd(II)-ATR. Soluble sugar contents firstly decreased and then increased with increasing concentrations of Cd(II)-ATR and displayed fluctuation with increasing concentrations of Pb(II)-ATR. Total protein contents increased with increasing concentrations of Cd(II) and Pb(II)-ATR, relative to the control sample. The NP-SH contents showed a significant increase up to 12. 2 mg/gfw of Cd(II)-ATR, followed by a significant decline to 4. 5 mg/gfw after 14 days of exposure. The effect of Pb(II)-ATR was similar to that of Cd(II)-ATR but the amount of NP-SH was not higher than that of Cd(II)-ATR. © 2011 Science Press, Institute of Geochemistry, CAS and Springer-Verlag Berlin Heidelberg. Source

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