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PubMed | Jiangsu Jiangyin Peoples Hospital, Taixing Peoples Hospital and Nanjing Southeast University
Type: Journal Article | Journal: American journal of physiology. Renal physiology | Year: 2015

Renal fibrosis is a histological outcome of chronic kidney disease (CKD) progression. However, the noninvasive detection of renal fibrosis remains a challenge. Here we constructed a renal fibrosis target mRNA array and used it to detect urinary mRNAs of CKD patients for investigating potential noninvasive biomarkers of renal fibrosis. We collected urine samples from 39 biopsy-proven CKD patients and 11 healthy controls in the training set. Urinary mRNA profiles of 86 genes showed a total of 21 mRNAs that were differentially expressed between CKD patients and controls (P < 0.05), and vimentin (VIM) mRNA demonstrated the highest change fold of 9.99 in CKD vs. controls with robust correlations with decline of renal function and severity of tubulointerstitial fibrosis. Additionally, VIM mRNA further differentiated patients with moderate-to-severe fibrosis from none-to-mild fibrosis group with an area of the curve of 0.796 (P = 0.008). A verification of VIM mRNA in the urine of an additional 96 patients and 20 controls showed that VIM is not only well correlated with renal function parameters but also correlated with proteinuria and renal fibrosis scores. Multiple logistic regression and receiver-operating characteristics analysis further showed that urine VIM mRNA is the best predictive parameter of renal fibrosis compared with estimated glomerular filtration rate, serum creatinine, and blood urea nitrogen. In addition, there is no improved predictive performance for the composite biomarkers to predict renal fibrosis severity compared with a single gene of VIM. Overall, urinary VIM mRNA might serve as a novel independent noninvasive biomarker to monitor the progression of kidney fibrosis.


Zhang H.,Jiangsu Jiangyin Peoples Hospital | Ma G.,Nanjing Southeast University | Yao Y.,Nanjing Southeast University | Qian H.,Jiangsu Jiangyin Peoples Hospital | And 4 more authors.
International Journal of Molecular Sciences | Year: 2012

Oxidized low density lipoprotein (ox-LDL) and its receptor, lectin-Like ox-LDL receptor-1 (LOX-1), play important roles in the development of endothelial injuries. Olmesartan can protect endothelial cells from the impairment caused by various pathological stimulations. In the present study we investigated whether olmesartan decreased the impairment of endothelial cells induced by ox-LDL by exerting its effects on LOX-1 both in vitro and in vivo. Incubation of cultured endothelial cells of neonatal rats with ox-LDL for 24 h or infusion of ox-LDL in mice for 3 weeks led to the remarkable impairment of endothelial cells, including increased lactate dehydrogenase synthesis, phosphorylation of p38 mitogen-activated protein kinases (p38 MAPK) and expression of apoptotic genes such as B-cell leukemia/lymphoma 2 (Bcl-2)-associated X protein (Bax) and caspase-3. Simultaneously, the cell vitality and expression of Bcl-2 gene were greatly reduced. All these effects, however, were significantly suppressed by the treatment with olmesartan. Furthermore, ox-LDL promoted up-regulation of LOX-1 expression either in cultured endothelial cells or in the aortas of mice, which was reversed with the administration of olmesartan. Our data indicated that olmesartan may attenuate the impairment of endothelial cell via down-regulation of the increased LOX-1 expression induced by ox-LDL. © 2012 by the authors; licensee MDPI, Basel, Switzerland.


Xu Y.,Soochow University of China | Xu Y.,Jiangsu Jiangyin Peoples Hospital | Zhao H.,Jiangsu Jiangyin Peoples Hospital | Hou J.,Soochow University of China
Tumor Biology | Year: 2014

The objective of this study was to investigate the role of epithelial cell adhesion molecule (EpCAM) in the genesis and the progress of prostate cancer, especially of castration-resistant prostate cancer. Protein expression of EpCAM in ten pairs of prostate cancer tissues and normal adjacent tissues, plus three cell lines, was examined. Short hairpin RNA (shRNA) interference technique was employed to silence the expression of EpCAM in prostate cancer cell LNCaP and construct a stable transfected cell line. In vitro assay was conducted to analyze the effect of EpCAM expression on the expressions of Androgen receptor (AR), Prostate specific antigen (PSA), and cellular proliferation and invasion. EpCAM was found significantly expressed higher in prostate cancer tissues than in normal adjacent tissues. In three cell lines (DU-145, PC-3, and LNCaP), the expression of EpCAM in LNCaP, androgen-dependent prostate cancer cells, was significantly higher than that in the other two. As EpCAM was silenced in LNCaP, the expression levels of AR and PSA obviously descended, and cellular abilities of proliferation and invasion were obviously inhibited.The overexpression of EpCAM has correlation with the genesis of prostate cancer, especially androgen-dependent prostate cancer. As the expression of AR is facilitated, prostate cancer cells' abilities to proliferate and invade are consequently enhanced. © 2014 International Society of Oncology and BioMarkers (ISOBM).


PubMed | Nantong Tumor Hospital, Nantong University and Jiangsu Jiangyin Peoples Hospital
Type: Journal Article | Journal: PloS one | Year: 2017

This study sought to identify factors associated with objectively assessed exercise behavior in Chinese patients with early-stage cancer. Three hundred and fifty one cancer patients were recruited from the Affiliated Jiangyin Hospital of Southeast University Medical College and the Nantong Tumor Hospital. One-way ANOVA, Pearson Chi-square tests and regression analysis were employed to identify the correlations between physical exercise and the measured factors. The results showed that occupation type (2 = 14.065; p = 0.029), monthly individual monthly income level (2 = 24.795; p = 0.003), BMI (2 = 15.709; p = 0.015) and diagnosis (2 = 42.442; p < 0.000) were significantly correlated with the subjects self-reported exercise with different frequency per week. Differences in the frequency of exercise were associated with different degrees of reported Benefit Finding (BF) (F = 24.651; p < 0.000), communication with doctors (F = 15.285; p < 0.000), medical coping modes (F = 45.912; p < 0.000), social support (F = 2.938; p = 0.030), depression (F = 6.017; p < 0.000), and quality of life (F = 12.288; p < 0.000). Multiple regression analysis showed that 1.6%-6.4% of the variance in five variables, excluding social support and optimism could be explained by exercise. Our results indicated that benefit finding, medical coping modes, communication with doctors, social support, depression and quality of life were significantly correlated with exercise. The variance in several psychosocial factors (benefit finding, medical coping modes, the communication with doctors, depression and quality of life) could be explained by exercise. Psychosocial factors should be addressed and examined over time when evaluating the effect of physical exercise that is prescribed as a clinically relevant treatment.


PubMed | Nantong University, Nantong Tumor Hospital, University of Miami and Jiangsu Jiangyin Peoples Hospital
Type: | Journal: European journal of oncology nursing : the official journal of European Oncology Nursing Society | Year: 2016

To adapt the Benefit Finding Scale (BFS) from English to a Chinese version after considering cross-cultural influences, to validate the tool for assessing benefit in Chinese-speaking patients with early-stage cancer, and to analyze the relationships between BF and sociodemographic and disease-related variables.In part I of the study, the Chinese BFS was adapted from the English version by developing guidelines after due consideration for cross-cultural influences. The translation validity index was used for assessing the quality of translation, and further refining was carried out by administering the scale on early-stage cancer patients (N1=200, N2=351) in part II and III. The Chinese version of the BFS was analyzed for reliability, dimensionality and construct validity. Relationships between BF and sociodemographic and disease-related variables were analyzed by ANOVA and regression models.The Chinese BFS was received favorably by patients during the preliminary testing. Cronbachs alpha and interclass correlation coefficients exceeded 0.7, and factor analysis yielded six factors. Convergent validity, discriminant validity and concurrent validity results indicated a satisfactory psychometric value. Sociodemographic and disease-related predictors of BF were observed.The Chinese BFS demonstrated good patient acceptability and exhibited strong psychometric properties among Chinese patients with early-stage cancer. There were differences between Chinese cancer patients benefit finding and patients with cancer in foreign countries, domestic cancer patients revealed high score in social relationship and family relationship dimension. Educational level, monthly individual income, treatment and physical exercise were the predictors of Chinese cancer patients benefit finding.


PubMed | Nanjing Medical University and Jiangsu Jiangyin Peoples Hospital
Type: Journal Article | Journal: Journal of thoracic disease | Year: 2017

Brain metastasis is an inauspicious consequence of lung cancer. However, the majority of cancer cells that seep into the brain died of unknown causes, only a few survived and developed into metastatic brain tumor. Communication between cancer cells and host tissue is viewed as an essential event during metastasis, but little is known about the accurate control of this processes. Within the lesion of brain metastasis, abundant activated astrocytes are observed with lung cancer cells. Previous studies have demonstrated that the astrocyte network served a protective role in the central nervous system (CNS) and most malignant cells that seep into the brain perish were rejected by astrocytes. Reactive astrocytes generated protease plasmin and cytotoxic cytokines as a defense against metastatic invasion. But recently, other investigators argued that tumor cells interactions with astrocytes promote the progression of brain metastases and protect them from the cytotoxic effects of chemotherapy. In this article, we review the architecture between astrocytes and infiltrated cancer cells, and raise a future perspective on therapeutic potential of targeting crosstalk modulators against brain metastasis.


Cao C.,Jiangsu Jiangyin Peoples Hospital | Zhang L.,Jiangsu Jiangyin Peoples Hospital
Cell Biochemistry and Biophysics | Year: 2015

The harm, the causes, and nursing care methods of lower respiratory tract infection after abdominal operation were described in this article. © 2015, Springer Science+Business Media New York.


Wu J.,Jiangsu Jiangyin Peoples Hospital | Zou L.,Jiangsu Jiangyin Peoples Hospital
Cell Biochemistry and Biophysics | Year: 2015

The aim of this study is to explore the efficacy of the 3C therapeutic regimen for type 1 diabetes. Thirty-nine patients with type 1 diabetes, who were hospitalized from January 2013 to April 2014, were included to receive 3C therapeutic regimen. Evidence-based nursing was performed in the treatment period and the efficacy was observed 6 days after therapy. Six days after the administration of the 3C therapeutic regimen, the fasting glucose levels in all 39 patients were controlled to be 4.4–6.0 mmol/L and 2h-postprandial glucose levels to be 4.4–7.8 mmol/L. Three patients had a glucose level <3.9 mmol/L, which was corrected after adjusting the dose of insulin infusion. Evidence-based nursing was provided in the treatment period and no nursing-associated complication occurred. All patients were satisfied with the nursing service. The efficacy of the 3C therapeutic regimen for type 1 diabetes is satisfactory. The evidence-based nursing can help to ensure the efficacy and improve the quality of nursing service. © 2014, Springer Science+Business Media New York.


PubMed | Jiangsu Jiangyin Peoples Hospital
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2016

The aim of this study is to explore the efficacy of the 3C therapeutic regimen for type 1 diabetes. Thirty-nine patients with type 1 diabetes, who were hospitalized from January 2013 to April 2014, were included to receive 3C therapeutic regimen. Evidence-based nursing was performed in the treatment period and the efficacy was observed 6 days after therapy. Six days after the administration of the 3C therapeutic regimen, the fasting glucose levels in all 39 patients were controlled to be 4.4-6.0 mmol/L and 2h-postprandial glucose levels to be 4.4-7.8 mmol/L. Three patients had a glucose level <3.9 mmol/L, which was corrected after adjusting the dose of insulin infusion. Evidence-based nursing was provided in the treatment period and no nursing-associated complication occurred. All patients were satisfied with the nursing service. The efficacy of the 3C therapeutic regimen for type 1 diabetes is satisfactory. The evidence-based nursing can help to ensure the efficacy and improve the quality of nursing service.


PubMed | Jiangsu Jiangyin Peoples Hospital
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2016

The harm, the causes, and nursing care methods of lower respiratory tract infection after abdominal operation were described in this article.

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