Hu B.-L.,Jiangxi Academy of Agricultural science |
Hu B.-L.,Jiangxi Normal University |
Li X.,Jiangxi Academy of Agricultural science |
Wan Y.,Jiangxi Academy of Agricultural science |
And 3 more authors.
Chinese Journal of Applied Ecology | Year: 2015
To identify the low nitrogen tolerance of Dongxiang wild rice (DXWR) and its progenies, ten phenotypic traits including plant height (PH), heading day (HD), panicle length (PL), number of effective tillers per plant (NETP), number of filled grains per panicle (NFGP), number of grains per panicle (NGP), grain density (GD), spikelet fertility (SF), 1000-grain mass (TGM) and grain yield per plant (GYP) were studied under normal and low nitrogen treatments, using backcross inbred lines (BILs) of Xieqingzao B//DXWR/Xieqingzao B in BC1F12. Comprehensive evaluation on the low nitrogen tolerance of the BILs population was conducted using principal component analysis and the subordinate function. The evaluation results indicated that the low nitrogen tolerance of the line 116, 143 and 157 was the strongest, which could be served as the intermediate materials for genetic studies on the low nitrogen tolerance of DXWR and breeding for the low nitrogen tolerance in rice. The optimal regression equation of the low nitrogen tolerance in rice was established using stepwise regression analysis. The relative values of five traits including PH, NGP, SF, TGM and GYP were screened out and could be used as comprehensive evaluation indices for the low nitrogen tolerance in the whole growth stage. Therefore, more attention should be paid to the relative values of these five traits, especially for NGP and GYP, in the genetic improvement of the low nitrogen tolerance in rice. ©, 2015, Editorial Board of Chinese Journal of Applied Ecology. All right reserved.
Peng X.,Nanchang University |
Ding X.,Nanchang University |
Chang T.,Nanchang University |
Wang Z.,Nanchang University |
And 4 more authors.
The Scientific World Journal | Year: 2014
High soils salinity is a main factor affecting agricultural production. Studying the function of salt-tolerance-related genes is essential to enhance crop tolerance to stress. Rab7 is a small GTP-binding protein that is distributed widely among eukaryotes. Endocytic trafficking mediated by Rab7 plays an important role in animal and yeast cells, but the current understanding of Rab7 in plants is still very limited. Herein, we isolated a vesicle trafficking gene, OsRab7, from rice. Transgenic rice over-expressing OsRab7 exhibited enhanced seedling growth and increased proline content under salt-treated conditions. Moreover, an increased number of vesicles was observed in the root tip of OsRab7 transgenic rice. The OsRab7 over-expression plants showed enhanced tolerance to salt stress, suggesting that vacuolar trafficking is important for salt tolerance in plants. © 2014 Xiaojue Peng et al.
Peng X.,Nanchang University |
Liu R.,Nanchang University |
Yang J.,Wuhan University |
Yang J.,State Key Laboratory of Hybrid Rice |
And 5 more authors.
Chinese Journal of Applied and Environmental Biology | Year: 2014
In order to study the pathway of rice response to abiotic stresses and discover the molecular mechanisms of stress tolerance in rice, we constructed a mixed cDNA library by using Gateway technology from rice roots subjected to salt and drought treatments. The quality assessment of this library showed that the titer of the unamplified library was 1.5 × 106 cfu, and the average insert size about 1 kb with recombination efficiency of 100%. In this study, randomly-selected clones were sequenced to find many functional genes, including glycosyl hydrolases, stress induced protein, oxidoreductase and glycinerich RNA-binding protein, etc. The results suggested that the mixed cDNA library from rice roots was successfully generated in high quality, and it may provide an essential resource for further stress-responsive gene cloning and functional study.