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Peng X.,Nanchang University | Ding X.,Nanchang University | Chang T.,Nanchang University | Wang Z.,Nanchang University | And 4 more authors.
The Scientific World Journal | Year: 2014

High soils salinity is a main factor affecting agricultural production. Studying the function of salt-tolerance-related genes is essential to enhance crop tolerance to stress. Rab7 is a small GTP-binding protein that is distributed widely among eukaryotes. Endocytic trafficking mediated by Rab7 plays an important role in animal and yeast cells, but the current understanding of Rab7 in plants is still very limited. Herein, we isolated a vesicle trafficking gene, OsRab7, from rice. Transgenic rice over-expressing OsRab7 exhibited enhanced seedling growth and increased proline content under salt-treated conditions. Moreover, an increased number of vesicles was observed in the root tip of OsRab7 transgenic rice. The OsRab7 over-expression plants showed enhanced tolerance to salt stress, suggesting that vacuolar trafficking is important for salt tolerance in plants. © 2014 Xiaojue Peng et al. Source


Peng X.,Nanchang University | Liu R.,Nanchang University | Yang J.,Wuhan University | Yang J.,State Key Laboratory of Hybrid Rice | And 5 more authors.
Chinese Journal of Applied and Environmental Biology | Year: 2014

In order to study the pathway of rice response to abiotic stresses and discover the molecular mechanisms of stress tolerance in rice, we constructed a mixed cDNA library by using Gateway technology from rice roots subjected to salt and drought treatments. The quality assessment of this library showed that the titer of the unamplified library was 1.5 × 106 cfu, and the average insert size about 1 kb with recombination efficiency of 100%. In this study, randomly-selected clones were sequenced to find many functional genes, including glycosyl hydrolases, stress induced protein, oxidoreductase and glycinerich RNA-binding protein, etc. The results suggested that the mixed cDNA library from rice roots was successfully generated in high quality, and it may provide an essential resource for further stress-responsive gene cloning and functional study. Source


Hu B.-L.,Jiangxi Academy of Agricultural science | Hu B.-L.,Jiangxi Normal University | Li X.,Jiangxi Academy of Agricultural science | Wan Y.,Jiangxi Academy of Agricultural science | And 3 more authors.
Chinese Journal of Applied Ecology | Year: 2015

To identify the low nitrogen tolerance of Dongxiang wild rice (DXWR) and its progenies, ten phenotypic traits including plant height (PH), heading day (HD), panicle length (PL), number of effective tillers per plant (NETP), number of filled grains per panicle (NFGP), number of grains per panicle (NGP), grain density (GD), spikelet fertility (SF), 1000-grain mass (TGM) and grain yield per plant (GYP) were studied under normal and low nitrogen treatments, using backcross inbred lines (BILs) of Xieqingzao B//DXWR/Xieqingzao B in BC1F12. Comprehensive evaluation on the low nitrogen tolerance of the BILs population was conducted using principal component analysis and the subordinate function. The evaluation results indicated that the low nitrogen tolerance of the line 116, 143 and 157 was the strongest, which could be served as the intermediate materials for genetic studies on the low nitrogen tolerance of DXWR and breeding for the low nitrogen tolerance in rice. The optimal regression equation of the low nitrogen tolerance in rice was established using stepwise regression analysis. The relative values of five traits including PH, NGP, SF, TGM and GYP were screened out and could be used as comprehensive evaluation indices for the low nitrogen tolerance in the whole growth stage. Therefore, more attention should be paid to the relative values of these five traits, especially for NGP and GYP, in the genetic improvement of the low nitrogen tolerance in rice. ©, 2015, Editorial Board of Chinese Journal of Applied Ecology. All right reserved. Source


Nie Y.-Y.,Jiangxi Agricultural University | Nie Y.-Y.,Jiangxi Super rice Research and Development Center | Zhang L.,Jiangxi Agricultural University | Wu Y.-H.,Jiangxi Agricultural University | And 11 more authors.
Ecology and Evolution | Year: 2015

Due to severe water resource shortage, genetics of and breeding for DT (drought tolerance) in rice (Oryza sativa L.) have become one of the hot research topics. Identification of grain yield QTLs (quantitative trait loci) directly related to the DT trait of rice can provide useful information for breeding new drought-resistant and water-saving rice varieties via marker-assisted selection. A population of 105 advanced BILs (backcross introgression lines) derived from a cross between Zhenshan97B and IRAT109 in Zhenshan97B background were grown under drought stress in a field experiment and phenotypic traits were investigated. The results showed that in the target interval of RM273-RM255 on chromosome 4, three main-effect QTLs related to panicle length, panicle number, and spikelet number per panicle were identified (LOD [logarithm of the odds] > 2.0). The panicle length-related QTL had two loci located in the neighboring intervals of RM17308-RM17305 and RM17349-RM17190, which explained 18.80% and 20.42%, respectively, of the phenotypic variation, while the panicle number-related QTL was identified in the interval of RM1354-RM17308, explaining 11.47% of the phenotypic variation. As far as the spikelet number per panicle-related QTL was concerned, it was found to be located in the interval of RM17308-RM17305, which explained 28.08% of the phenotypic variation. Using the online Plant-GE query system, a total of 13 matched ESTs (expressed sequence tags) were found in the target region, and of the 13 ESTs, 12 had corresponding predicted genes. For instance, the two ESTs CB096766 and CA765747 were corresponded to the same predicted gene LOC_Os04g46370, while the other four ESTs, CA754286, CB000011, CX056247, and CX056240, were corresponded to the same predicted gene LOC_Os04g46390. © 2015 Published by John Wiley & Sons Ltd. Source

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