Liu C.,Jiangxi Provinicial Peoples Hospital |
Xiong Q.-H.,Jiangxi Provinicial Peoples Hospital |
Fu J.-S.,Jiangxi Provinicial Peoples Hospital |
Liu S.-Q.,Jiangxi Provinicial Peoples Hospital |
Xiong L.,Jiangxi Provinicial Peoples Hospital
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2014
Objective: To construct the acellular artificial dermis loaded with nanoparticles that contain different ratios of platelet-derived growth factor-BB (PDGF-BB) and insulin-like growth factor-1 (IGF-1) for repairing skin wounds of nude mice and to explore the best ratio of PDGF-BB and IGF-1 for repairing skin wounds. Methods: Sustained released nanoparticles were made by enveloping different ratios of PDGF-BB and IGF-1 with polylactic acid-glycolic acid (PLGA) and then were combined with acellular artificial dermis. Nanoparticle loaded acellular artificial dermis was transplanted to the wound model of nude mice. Effects of nanoparticle loaded acellular artificial dermis of groups with different mass ratio of PDGF-BB and IGF-1 (1:4, 1:2, 1:1, 2:1, and 4:1)(n=5) on healing mice skin wounds were observed by recording the wound healing speed and the average healing time and by detecting the protein expressions of α-SMA and IL-2 in local tissues. In addition, the blank control group was also established. Results: When the mass ratio of PDGF-BB and IGF-1 of nanoparticle loaded acellular artificial dermis was 2:1, the wound healing speed was faster than that of the blank control group and groups with other mass ratios (P<0.05); the average healing time was shorter (P<0.05); and the protein expressions of α-SMA and IL-2 in local tissues were higher (P<0.05). Conclusion: When acellular artificial dermis loaded with nanoparticles that contain PDGF-BB and IGF-1 is used for skin wound healing, the best ratio of PDGF-BB and IGF-1 is 2:1. ©, 2014, Editorial Department of Journal of Shanghai Second Medical University. All right reserved.
Liu C.,Jiangxi Provinicial Peoples Hospital
Molecular Medicine Reports | Year: 2015
At least 50% of hypertrophic scarring (HS) is characterized by inflammation, for which there is currently no effective treatment available. Emodin is a major component of the widely used Chinese herb, rhubarb, which has been used to treat inflammation in several types of disease. However, few studies have investigated the efficacy of emodin in the treatment of HS. In the present study, a mouse model with mechanical stress-induced HS was used to investigate the effects of emodin (20, 40, 80, or 120 mg/ml) on HS, and to determine the potential underlying mechanisms. Treatment with emodin significantly attenuated HS inflammation, as determined by histopathological assessment of the scar elevation index, collagen structure and inflammation (P<0.05). Furthermore, treatment with emodin (40 mg/ml) markedly inhibited phosphoinositide 3-kinase (PI3K)/Akt activity (P<0.01) and this attenuation was associated with reduced expression levels of tumor necrosis factor-α, interleukin-6 and monocyte chemoattractant protein-1 (P<0.05) in the HS tissue. The results of the present study indicated that administration of emodin had therapeutic effects on the progression of HS and the underlying mechanism of this may be due to inhibition of the PI3K/Akt signaling pathway.