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PubMed | Jilin University, Jiangxi Provincial Tumor Hospital and Tongji University
Type: Journal Article | Journal: PloS one | Year: 2015

To investigate the prognostic significance of TGFR2 expression and chemotherapy in Chinese non-small cell lung cancer (NSCLC) patients, TGFR2 expression NSCLC was analyzed in silico using the Oncomine database, and subsequently analyzed with quantitative RT-PCR in 308 NSCLC biopsies, 42 of which were paired with adjacent non-neoplastic tissues. Our results show that TGFR2 expression was also increased in NSCLC biopsies relative to normal tissue samples and correlated with poor prognosis. TGFR2 expression was also significantly correlated with other clinical parameters such as tumor differentiation, invasion of lung membrane, and chemotherapy. Moreover, overall survival (OS) and disease free survival (DFS) was increased in patients with low TGFR2 expressing NSCLC and who had undergone chemotherapy. Thus, high expression of TGFR2 is a significant risk factor for decreased OS and DFS in NSCLC patients. Thus, TGFR2 is a potential prognostic tumor biomarker for chemotherapy.


Zhang H.-Q.,Jiangxi Provincial Tumor Hospital | He B.,Jiangxi Provincial Tumor Hospital | Fang N.,Nanchang University | Lu S.,Jiangxi Provincial Tumor Hospital | And 2 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2013

We aimed to investigate the mechanism and effects of autophagy on cisplatin (DDP)-induced apoptosis in human gastric cancer cell line SGC7901. After SGC7901 cells were treated with DDP and/or chloroquine, cell proliferation was measured using MTT assay; cell apoptosis was determined by flow cytometry; autophagy and apotosis-related proteins expression were detected by Western blot; and quantitative analysis of autophagy after monodansylcadaverine (MDC) staining was performed using fluorescence microscopy. We found after treatment with 5 mg/L DDP for 24 h, the rates of cell apoptosis were (21.07±2.12)%. Autophagy, characterized by an increase in the number of autophagic vesicles and the level of LC3-II protein was observed in cells treated with DDP. After inhibition of autophagy by chloroquine, the rates of cell apoptosis were increased to (30.16±3.54)%, and the level of Caspase-3 and P53 protein were increased, and Bcl-2 protein was decreased. Therefore, autophagy protects human gastric cancer cell line SGC7901 against DDP-induced apoptosis, inhibition of autophagy can promote apoptosis, and combination therapy with DDP and chloroquine may be a promising therapeutic strategy for gastric cancer.


Wang M.,Jiangxi Provincial Tumor Hospital | Wang Y.,Jiangxi Provincial Tumor Hospital | Zhong J.,Jiangxi Provincial Tumor Hospital
Molecular Medicine Reports | Year: 2015

Several studies have demonstrated that the isolated side population (SP) cells from solid tumors exhibit cancer stem cell-like properties, and are responsible for drug resistance during chemotherapy and tumor recurrence. In the current study, cancer stem cell-like SP cells were isolated in the MDU-22 breast cancer cell line using the Hoechst-33342 dye exclusion technique. It was observed that SP cells accounted for 3.8% of cells in the MDU-22 cell line, which was reduced to 0.6% in the presence of verapamil, an inhibitor of the ABC transporter. The sorted SP cells showed an elevated expression of stem cell markers, including ABCG2, OCT-4 and EpCAM. Furthermore, it was demonstrated that the isolated SP cells undergo rapid proliferation and have a high survival rate. These results indicate that the coexpression of adenosine triphosphatase binding cassette transporters and stem cell surface markers in SP cells may contribute to chemoresistance, tumor recurrence, metastasis and invasion. Therefore, the isolation and characterization of SP cells may provide novel insights for the development of alternative therapeutic agents to target cancer stem cells.


Xu Z.,Shandong University | Xu Z.,Jiangxi Provincial Tumor Hospital | Chen X.,Huazhong University of Science and Technology | Peng C.,Shandong University | And 4 more authors.
Hepato-Gastroenterology | Year: 2013

Background/Aims: To investigate Omi/HtrA2 expression and its clinical significance in hepatocellular carcinoma. Methodology: We analyzed Omi/HtrA2 expression in hepatocellular carcinoma, paracancerous tissues and normal hepatic tissues by immunohistochemistry, RT-PCR and Western blot. Hypoxia-inducible factor-1α (HIF-1α) expression was also detected in hepatocellular carcinoma samples by immunohistochemistry. Meanwhile, we retrospectively analyzed the relationship between Omi/HtrA2 expression and the survival times of the patients. Results: We found that Omi/HtrA2 overexpressed in hepatocellular carcinoma and was correlated with hepatocellular carcinoma differentiation, tumor size, portal vein invasion, clinical stage and lymph node metastasis. We also observed a significant inverse correlation between the expression of Omi/HtrA2 and HIF-1α in hepatocellular carcinoma. The Kaplan-Meier estimates showed that patients who were Omi/HtrA2 positive had much longer survival times than those who were Omi/HtrA2 negative. Both univariate and multivariate analysis using the Cox regression model indicated that Omi/HtrA2 expression was a significant factor for prognosis. Conclusions: Hepatocellular carcinoma cells may need Omi/HtrA2 expression for apoptosis and 0mi/HtrA2 might be an important prognostic marker for primary hepatocellular carcinoma. © H.G.E. Update Medical Publishing S.A.


PubMed | Jiangxi Provincial Tumor Hospital and Tongji University
Type: | Journal: Oncotarget | Year: 2016

To clarify the association between carboplatin and electrolyte abnormality, a pooled-analysis was performed with the adverse event reports of non-small cell lung cancer patients. A total of 19901 adverse events were retrieved from the FDA Adverse Event Reporting System (FAERS). Pooled reporting odds ratios (RORs) and 95% CIs suggested that carboplatin was significantly associated with hyponatremia (pooled ROR = 1.57, 95% CI 1.18-2.09, P = 1.9910-3) and hypokalemia (pooled ROR = 2.37, 95% CI 1.80-3.10, P = 5.2410-10) as compared to other therapies. In addition, we found that dehydration was frequently concurrent with carboplatin therapy (pooled ROR = 2.01, 95% CI 1.52-2.66, P = 8.3710-7), which may prompt excessive water ingestion and decrease serum electrolyte concentrations. This information has not been mentioned in the FDA-approved drug label and could help explain the physiological mechanism of carboplatin-induced electrolyte abnormality. In conclusion, the above results will facilitate clinical management and prompt intervention of life-threatening electrolyte imbalance in the course of cancer treatment.


PubMed | Nanchang University and Jiangxi Provincial Tumor Hospital
Type: Journal Article | Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] | Year: 2016

To construct a RNA interference lentiviral vector aimed at human ARK5 (AMPK-related protein kinase 5) gene and explore its effect on the biologic behavior of human gastric cancer SGC7901 cells.Targeting human ARK5 mRNA coding sequence, we designed three specific short hairpin RNAs (shRNAs) and constructed the lentiviral vector, then infected human gastric cancer SGC7901 cells with this vector. Afterwards, we used qPCR and Western blot for detecting the silencing effect on ARK5 gene, MTT colorimetric assay to measure the cell proliferation, cell scratch test for cell migration and Transwell for cell invasion, and flow cytometry analysis for apoptosis in cells treated with glucose starvation and TNF-.Sequencing proved that the recombinant lentiviral vector containing ARK5-shRNA-3 was constructed successfully. Real time fluorescent quantitative PCR assay showed that the expression abundance of ARK5 gene in the normal control group, negative control group and ARK5-shRNA-3 infected group were 1.002+ 0.082, 1.001+ 0.050 and 0.140+ 0.003, respectively, showing a statistically significant difference (P<0.01). Cell scratch test showed that the cell migration rate of ARK5-shRNA-3 infected group was (38.5+ 4.3)%, significantly lower than that of the normal control group [(72.4+ 6.4)%] and negative control group [(75.1+ 7.1)%, P<0.01]. The results of Transwell test showed that the number of penetrating cells in the normal control group, negative control group and ARK5-shRNA-3 transfection group were 257.412.3, 245.711.6, 112.57.8, with a significant difference (P<0.01). After glucose starvation and TNF--treatment for 24 h, the cell death rate of the normal control group, negative control group and ARK5-shRNA-3 group were (11.73.2)%, (12.32.6)% and (30.84.3)%, respectively, showing that the cell apoptosis rate of ARK5-shRNA-3 transfected group was significantly higher than that of the normal control and negative control groups (P<0.01).We have successfully constructed a recombinant lentiviral vector which can efficiently silence ARK5 gene. Using it we can inhibit the proliferation, migration, invasion of tumor cells, and promote cell apoptosis under the condition of TNF- treatment and glucose starvation.


PubMed | Jiangxi Provincial Tumor Hospital
Type: Journal Article | Journal: Asian Pacific journal of cancer prevention : APJCP | Year: 2013

We aimed to investigate the mechanism and effects of autophagy on cisplatin (DDP)-induced apoptosis in human gastric cancer cell line SGC7901. After SGC7901 cells were treated with DDP and/or chloroquine, cell proliferation was measured using MTT assay; cell apoptosis was determined by flow cytometry; autophagy and apotosis-related proteins expression were detected by Western blot; and quantitative analysis of autophagy after monodansylcadaverine (MDC) staining was performed using fluorescence microscopy. We found after treatment with 5 mg/L DDP for 24 h, the rates of cell apoptosis were (21.072.12)%. Autophagy, characterized by an increase in the number of autophagic vesicles and the level of LC3-II protein was observed in cells treated with DDP. After inhibition of autophagy by chloroquine, the rates of cell apoptosis were increased to (30.163.54)%, and the level of Caspase-3 and P53 protein were increased, and Bcl-2 protein was decreased. Therefore, autophagy protects human gastric cancer cell line SGC7901 against DDP-induced apoptosis, inhibition of autophagy can promote apoptosis, and combination therapy with DDP and chloroquine may be a promising therapeutic strategy for gastric cancer.


PubMed | Jiangxi Provincial Tumor Hospital
Type: Journal Article | Journal: Molecular medicine reports | Year: 2015

Several studies have demonstrated that the isolated side population (SP) cells from solid tumors exhibit cancer stem celllike properties, and are responsible for drug resistance during chemotherapy and tumor recurrence. In the current study, cancer stem celllike SP cells were isolated in the MDU22 breast cancer cell line using the Hoechst33342 dye exclusion technique. It was observed that SP cells accounted for3.8% of cells in the MDU22 cell line, which was reduced to 0.6% in the presence of verapamil, an inhibitor of the ABC transporter. The sorted SP cells showed an elevated expression of stem cell markers, including ABCG2, OCT4 and EpCAM. Furthermore, it was demonstrated that the isolated SP cells undergo rapid proliferation and have a high survival rate. These results indicate that the coexpression of adenosine triphosphatase binding cassette transporters and stem cell surface markers in SP cells may contribute to chemoresistance, tumor recurrence, metastasis and invasion. Therefore, the isolation and characterization of SP cells may provide novel insights for the development of alternative therapeutic agents to target cancer stem cells.


PubMed | Jiangxi Provincial Tumor Hospital and Shandong University
Type: Journal Article | Journal: Journal of laparoendoscopic & advanced surgical techniques. Part A | Year: 2015

Natural orifice translumenal endoscopic surgery (NOTES(); American Society for Gastrointestinal Endoscopy [Oak Brook, IL] and Society of American Gastrointestinal and Endoscopic Surgeons [Los Angeles, CA]) is an improvement in surgical interventions. In this study we developed an innovative transtracheal endoscopic thyroidectomy technique and explored its feasibility in animal models.Transtracheal endoscopic thyroidectomy was performed in anesthetized dogs and pigs. The endoscope was advanced into the pretracheal space via a longitudinal incision on the anterior tracheal wall. Hemithyroidectomies and partial lobectomy were performed using special double-lumen endotracheal tubes and conventional endoscopic instruments. The tracheal wall incision was closed using absorbable sutures, and the animals were sacrificed at Day 5 postsurgery.Hemithyroidectomy and partial thyroidectomy were successfully performed on pigs and dogs. The average operative time for each model was 69.4 minutes. No significant complications were encountered during surgery.The transtracheal endoscopic thyroidectomy technique is feasible and has the potential to be an alternative method for other types of thyroid surgeries.


PubMed | Nanchang University, Chinese PLA General Hospital and Jiangxi Provincial Tumor Hospital
Type: Journal Article | Journal: Diabetes/metabolism research and reviews | Year: 2015

Our aim is to clarify the features of complete type 2 diabetes mellitus (T2DM) remission in patients who undergo Roux-en Y gastric bypass surgery, to better determine factors affecting the outcome of T2DM surgery. A search was conducted for original studies on Medline, PubMed and Elsevier from inception until October 28, 2014. All of the articles included in this study were assessed with the application of predetermined selection criteria and were divided into two groups: Roux-en Y gastric bypass surgery for T2DM patients in remission or non-remission. The meta-analysis results demonstrated that fasting C-peptide values were significantly associated with increased remission (C-peptide: 95%CI=0.2-1.0) whereas T2DM duration, patient age, preoperative insulin use, preoperative fasting blood glucose values and preoperative glycosylated haemoglobin values were significantly associated with reduced remission (T2DM duration: 95%CI=-1.2 - -0.7; age: 95%CI=-0.5 - -0.1; percentage of preoperative insulin users: odd ratio=0.10, 95%CI=0.07-0.15; preoperative fasting blood glucose: 95%CI=-0.9 - -0.5; preoperative glycosylated haemoglobin: 95%CI=-1.1 - -0.4). However, the results demonstrated that body mass index was not statistically different (body mass index: 95%CI=-0.2-0.6). The results of the systematic review demonstrated that smaller waist circumference; lower total cholesterol, triglycerides and low-density lipoprotein levels, increased higher high-density lipoprotein levels, shorter cardiovascular disease history and less preoperative prevalence of hypertension contribute to the increased postoperative remission rate. Better results are obtained in younger patients with less severe diabetes, a smaller waist circumference, higher preoperative high-density lipoprotein, lower preoperative total cholesterol, triglycerides and low-density lipoprotein levels and fewer other complications of shorter durations.

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