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Yang Y.,Henan University | Yang J.,Henan University | Shi H.,Henan University | Chen Y.,Guangdong Nanxiong Tobacco Science Research Institute | And 4 more authors.
Acta Tabacaria Sinica | Year: 2015

Field experiments were carried out in 6 different tobacco growing regions producing strong flavored leaves from 2010-2013 to investigate the effect of climatic parameters at different transplanting dates during growing stage on tobacco quality and style. Relationship between climatic factors and tobacco quality and style was established by using correlation analysis. Results showed: (1) Such characters as flavor degree, smoke sinking degree and smoke concentration increased with the delay of transplanting date in southern tobacco areas, while such trend was the opposite in northern tobacco areas. (2) Significant positive correlation existed between daily average temperature at maturing stage and strong flavor degree, smoke sinking degree and smoke concentration. The average temperature of 24.5℃ at maturing stage was an important climate index for the formation of strong flavor tobacco style. (3) Burnt-sweetness flavor was strong in south tobacco areas and burnt flavor was strong in the north. Pure-sweetness flavor was obvious in southern Shaanxi and became stronger with the delay of transplanting date. (4) Tobacco quality varied significantly with different transplanting dates. Optimum transplanting time was in the early and middle of March for Nanxiong of Guangdong and Guangchang of Jiangxi, and in late March for South of Anhui, and in late April and early May for Northern tobacco areas. ©, 2015, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.


Lu X.,Hefei University of Technology | Lu X.,Suzhou University of Science and Technology | Gu H.,Jiangxi Provincial Tobacco Company | Chen Z.,Hefei University of Technology | And 4 more authors.
Acta Tabacaria Sinica | Year: 2015

Excess amount of Zinc is toxic and has negative effects on inhibition of growth, root development and may cause leaf chlorosis. The role of BABA in protecting tobacco seedling from high Zn2+ stress was studied and its working mechanism was explored through measuring antioxidative molecular content, antioxidative enzyme activities, concentration of Zn2+ in leaf and root and related gene expression. Results showed that 0.2 mmol/L BABA protected tobacco seedling from high-Zn2+ stress via increasing content of proline, GSH, total soluble protein and keeping MDA content at nearly normal level. Activities of CAT, POD and APX were reduced while SOD activity was increased, which raised signal molecule H2O2 content. BABA lowered Zn2+ concentration in leaf, but no obvious difference was found in root under high-Zn2+ stress by ICP-7300DV. In addition, gene expression indicated that the transcript level of PR1, PR2, NtMTP1A, NtMTP1B, NtC477 and NtYSL was higher than that of BABA untreated samples. It was suggested that the 0.2 mmol/L BABA was beneficial in alleviating high-Zn2+ stress in tobacco seedling by stimulating antioxidant system, restricting Zn2+ upper transportation and over expression genes of related ion transporters and pathogenesis-related proteins. © 2015, Acta Tabacaria Sinica Editorial Department. All right reserved.


Fu Y.,Henan Agricultural University | Liu Q.,Henan Agricultural University | Liu Q.,Jiangxi Provincial Tobacco Corporation | He X.,Yunnan Baoshan Oriental Tobacco Co. | And 2 more authors.
Acta Tabacaria Sinica | Year: 2013

Pot experiments of oriental tobacco plants were carried out to investigate effect of soil moisture on photosynthetic characteristics by using portable Li-6400 photosynthesis system during vigorous growing stage. Correlation analysis was conducted between environment factors and tobacco leaf characteristics, such as net photosynthesis rate and transpiration rate under different soil moisture levels. Results showed that (1) daily dynamics of Pn in oriental tobacco leaves under different soil moisture levels were all in the form of double peaks curves. Increase of soil moisture stress resulted in decline of peak value of Pn and midday depression intensified. (2) daily dynamics curves of Pn, Tr, Ci and Gs of oriental tobacco leaves showed significant differences under different soil moisture levels. (3) midday depression in oriental tobacco leaves may be controlled by both stomatal and non-stomatal factors. When soil moisture decline, non-stomatal is the main control factor. Correlation analysis indicated that Gs was key to Pn under three different treatments while air temperature under 65%-75% soil moisture and photosynthetic active radiation under 50%-60% and 35%-45% soil moisture levels were important to Tr. Maintaining relative soil moisture within 50%-60% may contribute to high levels of Pn and WUE.


Li C.,Jiangxi Provincial Tobacco Company | Tang M.,South China Agricultural University | Zhong Q.,Tobacco Research Institute of Ganzhou | He K.,Tobacco Leaf Institute of Jiangxi Province | And 5 more authors.
Tobacco Science and Technology | Year: 2013

In order to improve the accuracy of test results, the uncertainty in determination of quinclorac in soil with HPLC was systematically calculated according to JJF 1059-1999 Evaluation and expression of uncertainty in measurement. The results indicated that: 1) Preparation of standard working solution, calibration of working curve and constant volume of sample solution were the major factors influencing the uncertainty; 2) The combined uncertainty was 0.00069 mg/kg and the expanded uncertainty was 0.0014 mg/kg (level of confidence of 95%, k=2) at the concentration of quinclorac in soil of 0.0236 mg/kg. The uncertainty in determination of quinclorac in soil with HPLC could be decreased by using high purity standard substance and qualified metering devices, and strictly controlling treatment procedures.


Zhu K.,Hefei University of Technology | Peng Y.,Jiangxi Provincial Tobacco Company | Chen Z.,Hefei University of Technology | Zhang Q.,Hefei University of Technology | And 4 more authors.
Tobacco Science and Technology | Year: 2015

In order to alleviate the damage of copper stress on tobacco seedlings, the effects of β-aminobutyric acid (BABA) at different concentrations on the growth of seedlings, metabolism of reactive oxygen species (ROS) and copper ion transporter related-gene expression were investigated via experiments. The results showed that under 100 μmol/L copper stress, the root length and fresh weight of tobacco seedlings increased comparing with those of untreated seedlings. Under 0.5 mmol/L copper stress, 0.2 and 0.5 mmol/L BABA treatments decreased the contents of malonaldehyde (MDA) and hydrogen peroxide (H2O2), improved the activities of antioxidative enzymes (SOD, POD and APX), meanwhile the expression levels of NtNRAMP, NtYSL and NtPDR genes, which regulated the transportation of divalent metal ions in tobacco seedlings, significantly increased. It indicated that BABA alleviated the damage of tobacco caused by copper stress through regulating the antioxidant system and the expression levels of genes related to the transportation of metal ions. ©, 2015, Editorial Office of Tobacco Science and Technology. All right reserved.


Zhang Q.,Hefei University of Technology | Liu Z.,Jiangxi Provincial Tobacco Company | Zhong Y.,Jiangxi Provincial Tobacco Company | Wang W.,Anhui Chizhou Municipal Tobacco Company | And 4 more authors.
Acta Tabacaria Sinica | Year: 2015

This paper introduced the function of BABA in protecting tobacco from high salt stress and attempted to explore the mechanism through measuring antioxidative molecular content, antioxidative enzyme activities, and related gene expression. Results showed that 0.2 mmol/L BABA and 0.5 mmol/L BABA both could increase root length and fresh weight of tobacco under high salt stress significantly via increasing the contents of GSH, proline, chlorophyll, H2O2 and the activities of SOD, APX, POD and CAT. BABA alleviated the damage of cell membrane integrity via decreasing the contents of MDA and relative electrical conductivity. The involved ABA-response genes NtRAB18, NtERD10B, NtERD10D were higher expressed than the salt stress group. The above evidence suggested that 0.2 mmol/L BABA and 0.5 mmol/L BABA were beneficial in relieving high salt stress on tobacco by stimulating antioxidant system and over expression genes of ABA-response. ©, 2015, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.


Gu H.,Jiangxi Provincial Tobacco Corporation | He K.,Jiangxi Provincial Tobacco Corporation | Liu Z.,Jiangxi Provincial Tobacco Corporation | Xu Q.,Jiangxi Provincial Tobacco Corporation | And 2 more authors.
Acta Tabacaria Sinica | Year: 2015

Two disease-resistant and one susceptible tobacco varieties were used to determine variation of signal molecule SA and H2O2 in tobacco inoculated with TMV by the method of antibody and location staining. Results showed that inoculation with TMV could induce the rise of SA which reached maximum in 12h. SA content in the inoculated leaves rose higher than that in the same side upper leaves, and such difference was found among different varieties. TMV inoculation could also induce the rise of H2O2 and facilitate H2O2 spread from inside to outside while neither susceptible variety nor control group treated with PBS could. ©, 2015, State Tobacco Monopoly Bureau and China Tobacco Society. All right reserved.

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