Luo X.,University of Rochester |
Luo X.,Hangzhou Normal University |
Huang L.,Nanchang University |
Huang L.,Jiangxi Provincial Peoples Hospital |
And 7 more authors.
Schizophrenia Bulletin | Year: 2014
Recent genome-wide association studies have identified many promising schizophrenia candidate genes and demonstrated that common polygenic variation contributes to schizophrenia risk. However, whether these genes represent perturbations to a common but limited set of underlying molecular processes (pathways) that modulate risk to schizophrenia remains elusive, and it is not known whether these genes converge on common biological pathways (networks) or represent different pathways. In addition, the theoretical and genetic mechanisms underlying the strong genetic heterogeneity of schizophrenia remain largely unknown. Using 4 well-defined data sets that contain top schizophrenia susceptibility genes and applying protein-protein interaction (PPI) network analysis, we investigated the interactions among proteins encoded by top schizophrenia susceptibility genes. We found proteins encoded by top schizophrenia susceptibility genes formed a highly significant interconnected network, and, compared with random networks, these PPI networks are statistically highly significant for both direct connectivity and indirect connectivity. We further validated these results using empirical functional data (transcriptome data from a clinical sample). These highly significant findings indicate that top schizophrenia susceptibility genes encode proteins that significantly directly interacted and formed a densely interconnected network, suggesting perturbations of common underlying molecular processes or pathways that modulate risk to schizophrenia. Our findings that schizophrenia susceptibility genes encode a highly interconnected protein network may also provide a novel explanation for the observed genetic heterogeneity of schizophrenia, ie, mutation in any member of this molecular network will lead to same functional consequences that eventually contribute to risk of schizophrenia. © 2013 The Author.
Yang J.,First Peoples Hospital of Yunnan Province |
Lai P.,Jiangxi Provincial Peoples Hospital |
Wu D.,National Science Foundation |
Long Z.,National Science Foundation
Indian Journal of Ophthalmology | Year: 2014
Aims: To report the technique and outcomes of sutureless manual cataract extraction via a subconjunctival limbus oblique incision for mature cataracts. Materials and Methods: This retrospective study comprised of 112 eyes of 83 patients with mature cataract who all had manual cataract extraction via a subconjunctival limbus oblique incision. A transconjunctival tunnel is fashioned with a 3.0 mm keratome, 0.5 mm behind the limbal vascular arcades. A limbal tunnel, with a transverse extent of 9 mm in the cornea and 7.0 mm in the limbus, is created beneath the conjunctival/Tenon's tissue using an angled bevel-up crescent blade. Outcome measures included visual acuity, intraoperative complications, surgically induced astigmatism, endothelial cell loss rate and surgery time. Results: Self-sealing wound was achieved in 112 eyes (98.2%). The nucleus was delivered in whole in 108 eyes (96.4%). Intraoperative complications included hyphema in 3 eyes (2.7%), iridodialysis in 2 eyes 1.8%), posterior capsular rupture and zonular dialysis in 2 eyes (1.8%). At the 3-month follow-up, 91% patients achieved a best-corrected visual acuity of 20/20 or better, the mean of surgically induced astigmatism was -0.62 ± 0.41 Diopters and endothelial cell loss was 4.2%. Average surgical time was 3.75 min per case. Conclusion: This subconjunctival limbus oblique incision has the potential to serve as safe and effective technique for mature cataracts.
Tang Y.,Nanchang University |
Tang Y.,Jiangxi Provincial Peoples Hospital |
Zhong Z.,Nanchang University
Cell Biochemistry and Biophysics | Year: 2014
Clinical research has confirmed the efficacy of several plant extracts in the modulation of oxidative stress associated with hyperlipidemia and hyperglycemia induced by obesity and diabetes. Findings indicate that obtusifolin has antioxidant properties. The aim of this study was to evaluate the possible protective effects of obtusifolin against oxidative damage in diabetic hyperlipidemia and hyperglycemia. In this study, the rats were divided into the following groups with eight animals in each: control, untreated diabetic, three obtusifolin (10, 30, and 90 mg/kg/day)-treated diabetic groups. Diabetes was induced by streptozotocin (STZ) in rats. STZ was injected intraperitoneally at a single dose of 60 mg/kg for diabetes induction. Obtusifolin (intraperitoneal injection) was administered 3 days after STZ administration; these injections were continued to the end of the study (4 weeks). At the end of the 4-week period, blood was drawn for biochemical assays. In order to determine the changes of cellular antioxidant defense systems, antioxidant enzymes including glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) activities were measured in serum. Moreover, we also measured serum nitric oxide (NO) and serum malondialdehyde (MDA) levels, markers of lipid peroxidation. STZ-induced diabetes caused an elevation (P < 0.001) of blood glucose, MDA, NO, total lipids, triglycerides and cholesterol, with reduction of GSH level and CAT and SOD activities. The results indicated that the significant elevation in the blood glucose, MDA, NO, total lipids, triglycerides and cholesterol; also the reduction of glutathione level and CAT and SOD activity were ameliorated in the obtusifolin-treated diabetic groups compared with the untreated groups, in a dose-dependent manner (P < 0.05, P < 0.01, P < 0.001). These results suggest that obtusifolin has antioxidant properties and improves chemically induced diabetes and its complications by modulation of oxidative stress. © 2014, Springer Science+Business Media New York.
Zhou X.,Jiangxi Normal University |
Luo P.,Jiangxi Provincial Peoples Hospital |
Long L.,Jiangxi Normal University |
Ouyang M.,Jiangxi Normal University |
And 2 more authors.
Tetrahedron | Year: 2014
Ru-catalyzed direct C-H amidation of 2-arylbenzo[d]thiazoles was developed using sulfonyl azides as the amino source under no external oxidants and free-base conditions to release N2gas as the single byproduct. The present protocol shows good functional group tolerance and high regioselectivity, providing various structurally versatile amidated 2-arylbenzothiazoles with potential biological and therapeutic activities in moderate to good yields. © 2014 Elsevier Ltd. All rights reserved.
Liu T.,Southern Medical University |
Xue W.,Jinan University |
Ke B.,Jiangxi Provincial Peoples Hospital |
Xie M.-Q.,Southern Medical University |
Ma D.,Jinan University
Biomaterials | Year: 2014
A new cyclodextrin derivative (CD-PLLD) consisting of a β-cyclodextrin core and poly(l-lysine) dendron arms was prepared by the click conjugation of per-6-azido-b-cyclodextrin with propargyl focal point poly(l-lysine) dendron of third generation, and then used for docetaxel (DOC) and the best siRNA plasmid targeting MMP-9 (pMR3) co-delivery. Different from commonly used amphiphilic copolymers with cationic character, the as obtained cyclodextrin derivative may be used directly for the combinatorial delivery of nucleic acid and lipophilic anticancer drugs without a complicated micellization process. It was found that CD-PLLD/pMR3 nanocomplex showed a good gene transfection efficiency invitro, and could mediate the reduce of MMP-9 protein in HNE-1 cells. For co-delivery analysis, the obtained CD-PLLD/DOC/pMR3 complexes could induce a more significant apoptosis than DOC or pMR3 used only, and decreased invasive capacity of HNE-1 cells. Moreover, the star-shaped copolymer exhibited better blood compatibility and lower cytotoxicity compared to PEI-25k in the hemolysis and MTT assays, which should be encouraged in nasopharyngeal cancer therapy. © 2014 Elsevier Ltd.
Masaki K.,Neurological Institute |
Suzuki S.O.,Neurological Institute |
Matsushita T.,Neurological Institute |
Yonekawa T.,Neurological Institute |
And 7 more authors.
Acta Neuropathologica | Year: 2012
Extensive aquaporin-4 (AQP4) loss without perivascular deposition of either activated complement or immunoglobulins is a characteristic of Baló's disease. Our aim in this study was to investigate the relationship between astrocytopathy and demyelination in Baló's disease, focusing on connexins (Cx), which form gap junctions among glial cells and myelin. Autopsied specimens from four cases that provided seven actively demyelinating concentric lesions infiltrated with numerous CD68 + macrophages were immunohistochemically examined for the astrocyte markers glial fibrillary acidic protein (GFAP), AQP4, Cx43, Cx30 and megalencephalic leukoencephalopathy with subcortical cyst 1 (MLC1). Specimens were also stained for oligodendrocyte/ myelin markers, namely Cx32, Cx47, myelin-associated glycoprotein (MAG), myelin oligodendrocyte glycoprotein (MOG), oligodendrocyte-specific protein (OSP) and Nogo-A. Serum samples from six patients that had undergone magnetic resonance imaging, confirming a diagnosis of Baló's disease, were assayed for the presence of anti-Cx43, -Cx32 and -AQP4 antibodies. Despite the presence of numerous GFAP- and MLC1-positive astrocytes, there was a marked decrease in the levels of Cx43, Cx32 and Cx47. At the leading edges, Cx43 and AQP4 were mostly absent despite positive GFAP, MLC1, Cx32, Cx47, MOG, MAG, and OSP immunoreactivity. Of the six Baló's disease patients, none were positive for anti-Cxs or -AQP4 antibodies. Baló's disease is characterized by extensive loss of Cxs and AQP4, and a lack of auto-antibodies to Cxs and AQP4. Loss of Cx43 and AQP4 in the presence of other oligodendrocyte/myelin proteins at the leading edges suggests the possibility that auto-antibody-independent astrocytopathy may contribute to disease pathology via the disruption of astrocyte-oligodendrocyte/myelin interactions. © Springer-Verlag 2012.
Fu D.,Huazhong University of Science and Technology |
Zhou L.,Jiangxi Provincial Peoples Hospital |
Yang S.,Huazhong University of Science and Technology |
Xiao B.,Huazhong University of Science and Technology
Clinical Orthopaedics and Related Research | Year: 2013
Background: Various authors have proposed flaps to reconstruct traumatic forefoot skin and soft tissue defects, especially with exposure of tendon and/or bone although which is best for particular circumstances is unclear. Description of Technique: The indications for the technique were a forefoot defect area of no more than 8-cm × 8-cm and a well-preserved lateral tarsal (LT) donor site. The injured tendons were repaired using tendon grafts. The free dorsalis pedis flap was outlined by centering it on the cutaneous branch of the LT artery and tailoring it to the size of the wound, allowing 0.5-cm margins in length and width. The flap was rotated around the plantar perforating branch of the dorsalis pedis artery (DPA) to cover the forefoot defect. The lateral dorsalis pedis cutaneous nerve was anastomosed with the recipient plantar nerve stump. The donor site was covered with an inguinal, full-thickness skin graft. Patients and Methods: Traumatic forefoot skin and soft tissue defects with exposure of the tendon and/or bone involving 11 feet in 11 patients (mean age, 32 years) were covered using a LT flap with a reversed DPA pedicle. Three patients with forefoot defects underwent emergency repair within 8 hours of injury, whereas eight patients required delayed repair. All patients were followed up for at least 6 months (mean, 13 months; range, 6-24 months). Results: All flaps survived uneventfully, except for two that had superficial marginal necrosis or severe venous insufficiency. All skin grafts covering the donor sites survived and all wounds healed. None of the patients had restricted standing or walking at followups. The two-point discrimination was 4 mm to 10 mm at 6 months postoperative. The mean hallux- metatarsophalangeal-interphalangeal scale score was 93 points (range, 87-98 points). Conclusions: Our observations suggest the LT flap with a reversed DPA pedicle is a reasonable option for repair of traumatic forefoot skin and soft tissue defects with exposure of tendon and/or bone but a well-preserved LT donor site and is associated with minimal morbidity. Level of Evidence: Level IV, therapeutic study. See Guidelines for Authors for a complete description of levels of evidence. © 2012 The Association of Bone and Joint Surgeons®.
Li Q.G.,Jiangxi Provincial Peoples Hospital
Genetics and molecular research : GMR | Year: 2012
We examined the underlying neural-endocrine mechanisms of asthma associated with respiratory syncytial virus infection. Thirty Sprague-Dawley rats were randomly divided into control group, respiratory syncytial virus (RSV) group, and anti-nerve growth factor (NGF) IgG group. An RSV infection model was established by nasal drip once a week. In the anti-NGF antibody intervention group, each rat was given an intraperitoneal injection of anti-NGF IgG 3 h before RSV infection. Optical microscopy and transmission electron microscopy were used to observe the structural changes in adrenal medulla cells. Changes in adrenaline and norepinephrine in serum were detected by ELISA. NGF expression was assayed by immunohistochemistry. Expression differences in synaptophysin mRNA were detected by RT-PCR. Transmission electron microscopy displayed widened adrenal medulla intercellular spaces, reduced chromaffin particle concentration, and increased mitochondria in the RSV infection group. At the same time, NGF expression was increased in the RSV infection group significantly. In addition, the adrenaline concentration was significantly decreased compared with the control and anti-NGF antibody groups. Synaptophysin mRNA expression was significantly increased in the RSV infection and anti-NGF antibody groups. However, compared with the RSV infection group, synaptophysin mRNA expression was significantly decreased in the anti-NGF antibody group. We conclude that RSV infection could induce adrenal medulla cell differentiation to nerve cells by over-expression of NGF, resulting in the decreased endocrine function found in asthma progression.
Jin C.H.,Jiangxi Provincial Peoples Hospital
Zhonghua xin xue guan bing za zhi [Chinese journal of cardiovascular diseases] | Year: 2010
To characterize the differential and proliferative activities of FLK-1(+) cells derived from mouse fetal liver. The FLK-1(+) fraction were enriched from the fetal liver using immunomagnetic method and their differential and proliferative activities were examined in culture medium and in vivo via transplantation of FLK-1(+) cells into the inferior pole of the spleen of nine-week-old male C57 BL/6 mice after two-thirds hepatectomy. In response to growth factors, FLK-1(+) cells expressed typical lineage-specific markers for vascular endothelial cells, smooth muscle cells. Intrahepatic implantation of FLK-1(+) cells resulted in the formation of blood vessels in the fibrous capsule of partially hepatectomized liver. These results indicate that FLK-1(+) cells derived from mouse fetal liver could differentiate into endothelial cells and smooth muscle cells and they may serve as potential stem cells for clinical cell-based therapy.
Zhang C.Q.,Jiangxi Provincial Peoples Hospital
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2010
To explore the effects of Tongxinluo Capsule (TXLC) on platelet activating factor, vascular inflammation factor and vascular endothelial function in patients with essential hypertension (EH) complicated with diabetes mellitus (DM). One hundred patients of EH with DM were equally assigned to the TXLC group (treated by TXLC) and the control group (treated with the conventional therapy). Their fasting blood drawn from the cubital vein on the next morning of hospitalization was taken for determining serum level of high sensitivity C-reactive protein (hs-CRP) by emulsion immunoenhancement turbidimetry; plasmal fibrinogen C (FIB-C) by diffusive turbidimetry; platelet activating indices, CD62p and glucose protein (GP) II b/III a receptor complex by flow cytometry; endothelin-1 (ET-1) by radioimmunoassay and nitrogen oxide (NO) content by enzyme method. The outcomes were compared with those of 50 healthy persons. After patients were treated for 8 weeks, all the above-mentioned indices were reexamined and compared between groups. Results Blood levels of hs-CRP, FIB-C, CD62p, GP II b/IIIa and ET-1 in patients were significantly higher than those in healthy persons (all P < 0.01). All the indices as well as the blood pressure (both systolic and diastolic) reduced in patients of both groups significantly (P < 0.05 or P < 0.01), but the reducing was more significant in the TXLC group than in the control group. Besides, level of NO significantly increased in the TXLC group (P < 0.05). TXLC can inhibit the platelet activation and vascular inflammation response, also improve the vascular endothelial function in patients with EH complicated with DM. It may play a certain role in preventing and treatment of the occurrence of thrombotic complications in them.