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Qian J.,Jiangsu University | Qian J.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production | Zhou C.,Jiangsu University | Ma H.,Jiangsu University | And 5 more authors.
Food Biophysics | Year: 2016

Pulsed magnetic field (PMF) technology has emerged as a non-thermal method for inhibition of spoilage microorganism in food. In this study, we evaluate the effect of PMF treatment on the inactivation of Bacillus subtilis. The mechanisms responsible for cell death were also studied using transmission electron microscopy (TEM) and proteome approaches. Results showed that the survival rate of B. subtilis generally decreased with an increase of pulse numbers at the intensity of 3.30 T. The observation of TEM showed damage in cell cytoplasm and cytoplasmic membrane after PMF treatment. Additionally, 18 differentially expressed protein spots were identified by two dimensional gel electrophoresis (2D-GE) and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) analysis. The down-regulated outer membrane protein A (OmpA) illustrated that PMF destroyed the cell membrane. Furthermore, Gene ontology (GO) analysis and kyoto encyclopedia of genes and genomes (KEGG) pathway analysis were used to characterize the functions of those proteins. That PMF treatment damaged the membrane component, depressed cellular molecular functions and biological process, and decreased the carbohydrate metabolism and energy metabolism, which explain the death of cells. The presented results give the better view into the proteome of food microorganism and provide insight into the nature of PMF inactivation mechanisms. © 2016 Springer Science+Business Media New York Source


Cui F.,Jiangsu University | Cui F.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production | Li X.,Jiangsu University | Zhou Y.,China Agricultural University | And 5 more authors.
Chinese Journal of Environmental Engineering | Year: 2013

The present paper focused on the effect of NaOH pretreatment on anaerobic digestion (AD) of corn stover. NaOH solution with the concentrations of 1.0%, 2.5%, 5.0%, 7.5%(M/V) was used for pretreating corn stover with 24-soaked or no-soaked status, and the biogas production effeciency was determined during the AD process. The obtained data showed that lignin of corn stover was degraded up to 38.54% by 5.0% NaOH pretreatment for 24 h. The total gas yield was 226.75 L/kg VS, which was 38.54% higher than the group without NaOH pretreatment. All the results indicated that the pretreatment of corn stover by 5.0% NaOH for 24 h was preferred for further study due to its significant improvement of biogas prodution and efficiency of anaerobic digestion. Source


Cui F.-J.,Jiangsu University | Cui F.-J.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production | Chen X.-X.,Jiangsu University | Liu W.-M.,Jiangsu University | And 4 more authors.
Applied Biochemistry and Biotechnology | Year: 2016

The present study describes the improved mycelia and exo-polymer production under control of Grifola frondosa morphology by changing the aeration rate and agitation intensity in a 25-L stirred fermentor. The aeration rate of 1.0 vvm yielded a highest mycelia biomass of 24.754 g/L with the lowest pellet percentage of 20.5 %. The maximum exo-polymer (2.324 g/L) was achieved at 0.75 vvm with mycelia polysaccharide production (0.321 g/g), whereas clumps and filaments dominated the ratios of 45.6 and 33.9 %, respectively. The change of aeration rate and agitation intensity had slight influence on the monosaccharide compositions in exo-polymers and significantly affected glucose and mannose contents in the mycelia polysaccharides. These findings will provide a clue for exploring the relationship between fermentation parameters, morphologies, and polysaccharide synthesis pathway of G. frondosa. © 2016 Springer Science+Business Media New York Source


Cui F.,Jiangsu University | Cui F.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production | Zan X.,Jiangsu University | Li Y.,Jiangsu University | And 5 more authors.
Nutrition and Cancer | Year: 2016

GFG-3a is a novel glycoprotein previously purified from the fermented mycelia of Grifola frondosa with novel sugar compositions and protein sequencing. The present study aims to investigate its effects on the cell cycle, differential proteins expression, and apoptosis of human gastric cancer SGC-7901 cells. Our findings revealed that GFG-3a induced the cell apoptosis and arrested cell cycle at S phase. GFG-3a treatment resulted in the differential expression of 21 proteins in SGC-7901 cells by upregulating 10 proteins including RBBP4 associated with cell cycle arrest and downregulating 11 proteins including RUVBL1, NPM, HSP90AB1, and GRP78 involved in apoptosis and stress response. qRT-PCR and Western blot analysis also suggested that GFG-3a could increase the expressions of Caspase-8/-3, p53, Bax, and Bad while decrease the expressions of Bcl2, Bcl-xl, PI3K, and Akt1. These results indicated that the stress response, p53-dependent mitochondrial-mediated, Caspase-8/-3-dependent, and PI3k/Akt pathways were involved in the GFG-3a-induced apoptosis process in SGC-7901 cells. These findings might provide a basis to prevent or treat human gastric cancer with GFG-3a and understand the tumor-inhibitory molecular mechanisms of mushroom glycoproteins. © 2016 Taylor & Francis Group, LLC. Source


Sun W.-J.,Jiangsu University | Sun W.-J.,Parchn Sodium Isovitamin C Co. | Sun W.-J.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production | Yun Q.-Q.,Jiangsu University | And 10 more authors.
Biochemical Engineering Journal | Year: 2013

A continuous fermentation process for 2-keto-gluconic acid (2KGA) production from cheap raw material corn starch hydrolysate was developed using the strain Pseudomonas fluorescens AR4. The dilution rate and feeding glucose concentration had a significant effect on the cell concentrations, glucose utilization and 2KGA production performance. The optimal operating factors were obtained as: 0.065h-1 of dilution rate, 180g/L of feeding glucose concentration, and 16h of batch fermentation time as the starting point. Under these conditions, the steady state had the 135.92g/L of produced 2KGA concentration, 8.83g/L.h of average volumetric productivity, and 0.9510g/g of yield. In conclusion, the proposed efficient and stable continuous fermentation process for 2KGA production by the strain P. fluorescens AR4 is potentially competitive for industrial production from corn starch hydrolysate in terms of 2KGA productivity and yield. © 2013 Elsevier B.V. Source

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