Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production

and Technology, China

Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production

and Technology, China

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Cui F.,Jiangsu University | Cui F.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio Production | Li X.,Jiangsu University | Zhou Y.,China Agricultural University | And 5 more authors.
Chinese Journal of Environmental Engineering | Year: 2013

The present paper focused on the effect of NaOH pretreatment on anaerobic digestion (AD) of corn stover. NaOH solution with the concentrations of 1.0%, 2.5%, 5.0%, 7.5%(M/V) was used for pretreating corn stover with 24-soaked or no-soaked status, and the biogas production effeciency was determined during the AD process. The obtained data showed that lignin of corn stover was degraded up to 38.54% by 5.0% NaOH pretreatment for 24 h. The total gas yield was 226.75 L/kg VS, which was 38.54% higher than the group without NaOH pretreatment. All the results indicated that the pretreatment of corn stover by 5.0% NaOH for 24 h was preferred for further study due to its significant improvement of biogas prodution and efficiency of anaerobic digestion.


Cui F.-J.,Jiangsu University | Cui F.-J.,China Agricultural University | Zan X.-Y.,Jiangsu University | Zhou Y.-G.,China Agricultural University | And 5 more authors.
Drying Technology | Year: 2013

GFPS1b is an antitumor glycoprotein isolated and purified from the mycelia of Grifola frondosa GF9801. In the present study, freeze drying and hot air drying of GFPS1b were compared according to their drying kinetics, functional/conformational/topographical changes, and anti-proliferative activities for the first time. UV spectroscopy, FT-IR, SEM, and compositional analysis were conducted to evaluate the structural changes of GFPS1b after the above drying processes. Results showed that physicochemical and anti-proliferative properties of GFPS1b differed from each other after the two drying treatments. The freeze-dried GFPS1b-F showed the significant advantages over hot-air-dried GFPS1b-A due to its higher product yield, higher protein content, total sugar content, uronic acid content, higher solubility, and anti-proliferative activity. This study revealed that different drying methods used for preparation of glycoprotein GFPS1b could significantly affect the physicochemical properties and structure, which in turn adversely affect the biological activity of GFPS1b. © 2013 Copyright Taylor and Francis Group, LLC.


Sun W.-J.,Jiangsu University | Sun W.-J.,Parchn Sodium Isovitamin C Co. | Sun W.-J.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio Production | Yun Q.-Q.,Jiangsu University | And 10 more authors.
Biochemical Engineering Journal | Year: 2013

A continuous fermentation process for 2-keto-gluconic acid (2KGA) production from cheap raw material corn starch hydrolysate was developed using the strain Pseudomonas fluorescens AR4. The dilution rate and feeding glucose concentration had a significant effect on the cell concentrations, glucose utilization and 2KGA production performance. The optimal operating factors were obtained as: 0.065h-1 of dilution rate, 180g/L of feeding glucose concentration, and 16h of batch fermentation time as the starting point. Under these conditions, the steady state had the 135.92g/L of produced 2KGA concentration, 8.83g/L.h of average volumetric productivity, and 0.9510g/g of yield. In conclusion, the proposed efficient and stable continuous fermentation process for 2KGA production by the strain P. fluorescens AR4 is potentially competitive for industrial production from corn starch hydrolysate in terms of 2KGA productivity and yield. © 2013 Elsevier B.V.


Cui F.,Jiangsu University | Cui F.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production | Zan X.,Jiangsu University | Li Y.,Jiangsu University | And 5 more authors.
Nutrition and Cancer | Year: 2016

GFG-3a is a novel glycoprotein previously purified from the fermented mycelia of Grifola frondosa with novel sugar compositions and protein sequencing. The present study aims to investigate its effects on the cell cycle, differential proteins expression, and apoptosis of human gastric cancer SGC-7901 cells. Our findings revealed that GFG-3a induced the cell apoptosis and arrested cell cycle at S phase. GFG-3a treatment resulted in the differential expression of 21 proteins in SGC-7901 cells by upregulating 10 proteins including RBBP4 associated with cell cycle arrest and downregulating 11 proteins including RUVBL1, NPM, HSP90AB1, and GRP78 involved in apoptosis and stress response. qRT-PCR and Western blot analysis also suggested that GFG-3a could increase the expressions of Caspase-8/-3, p53, Bax, and Bad while decrease the expressions of Bcl2, Bcl-xl, PI3K, and Akt1. These results indicated that the stress response, p53-dependent mitochondrial-mediated, Caspase-8/-3-dependent, and PI3k/Akt pathways were involved in the GFG-3a-induced apoptosis process in SGC-7901 cells. These findings might provide a basis to prevent or treat human gastric cancer with GFG-3a and understand the tumor-inhibitory molecular mechanisms of mushroom glycoproteins. © 2016 Taylor & Francis Group, LLC.


Qian J.,Jiangsu University | Qian J.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production | Zhou C.,Jiangsu University | Ma H.,Jiangsu University | And 5 more authors.
Food Biophysics | Year: 2016

Pulsed magnetic field (PMF) technology has emerged as a non-thermal method for inhibition of spoilage microorganism in food. In this study, we evaluate the effect of PMF treatment on the inactivation of Bacillus subtilis. The mechanisms responsible for cell death were also studied using transmission electron microscopy (TEM) and proteome approaches. Results showed that the survival rate of B. subtilis generally decreased with an increase of pulse numbers at the intensity of 3.30 T. The observation of TEM showed damage in cell cytoplasm and cytoplasmic membrane after PMF treatment. Additionally, 18 differentially expressed protein spots were identified by two dimensional gel electrophoresis (2D-GE) and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) analysis. The down-regulated outer membrane protein A (OmpA) illustrated that PMF destroyed the cell membrane. Furthermore, Gene ontology (GO) analysis and kyoto encyclopedia of genes and genomes (KEGG) pathway analysis were used to characterize the functions of those proteins. That PMF treatment damaged the membrane component, depressed cellular molecular functions and biological process, and decreased the carbohydrate metabolism and energy metabolism, which explain the death of cells. The presented results give the better view into the proteome of food microorganism and provide insight into the nature of PMF inactivation mechanisms. © 2016 Springer Science+Business Media New York


Cui F.-J.,Jiangsu University | Cui F.-J.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production | Li Y.-H.,Jiangsu University | Zan X.-Y.,Jiangsu University | And 6 more authors.
Process Biochemistry | Year: 2014

HEG-5, a novel glycoprotein with hemagglutinating activity, was firstly isolated and purified from the cultured mycelia of Hericium erinaceus CZ-2. SDS-PAGE, Native-PAGE and MALDI-TOF-MS proved that HEG-5 was a single band with the molecular weight of approximately 14.4 kDa. HEG-5 had the protein: polysaccharide ratio of approximately 10:1 (%/%) and contained d-glucose, l-rhamnose, d-galactose and d-mannose with a molar ratio of 1.00:1.09:2.45:7.14 in polysaccharide fraction. HEG-5 was an acidic glycoprotein with a PI value of 6.3 and the higher content of acidic amino acids (Asp, 12.42 ± 0.25% and Glu, 12.24 ± 0.26%) in protein fraction. FT-IR and NMR spectra revealed that HEG-5 contained the protein and carbohydrate portions with (1→4)-linked β-galactose residues and β-linked glucose residues. Circular dichroism (CD) demonstrated that HEG-5 was a β-sheet predominant glycoprotein. Hemagglutination assay proved it was a thermo-unstable glycoprotein. The HEG-5 structural novelty was finally presented by protein sequencing and modeling by using MALDI-TOF-MS, NCBI blast search and online SWISS-MODEL Workspace service. © 2014 Elsevier Ltd.


Cui F.-J.,Jiangsu University | Cui F.-J.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production | Chen X.-X.,Jiangsu University | Liu W.-M.,Jiangsu University | And 4 more authors.
Applied Biochemistry and Biotechnology | Year: 2016

The present study describes the improved mycelia and exo-polymer production under control of Grifola frondosa morphology by changing the aeration rate and agitation intensity in a 25-L stirred fermentor. The aeration rate of 1.0 vvm yielded a highest mycelia biomass of 24.754 g/L with the lowest pellet percentage of 20.5 %. The maximum exo-polymer (2.324 g/L) was achieved at 0.75 vvm with mycelia polysaccharide production (0.321 g/g), whereas clumps and filaments dominated the ratios of 45.6 and 33.9 %, respectively. The change of aeration rate and agitation intensity had slight influence on the monosaccharide compositions in exo-polymers and significantly affected glucose and mannose contents in the mycelia polysaccharides. These findings will provide a clue for exploring the relationship between fermentation parameters, morphologies, and polysaccharide synthesis pathway of G. frondosa. © 2016 Springer Science+Business Media New York


Cui F.,Jiangsu University | Cui F.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production | Li Y.,Jiangsu University | Yang Y.,Shanghai Academy of Agricultural science | And 4 more authors.
Journal of Agricultural and Food Chemistry | Year: 2014

The present study investigated the changes of the chemical components and cytotoxicity potency at 5 developmental stages of Pleurotus eryngii fruiting body. The carbohydrate and protein contents increased along the maturity of fruiting body while fat content decreased. By comparison, the polysaccharide-protein fractions had the highest antiproliferative effect on SGC-7901 and HepG-2 cells in vitro and increasing activity with growing maturity of P. eryngii fruiting body.The maturation process increased the protein content and acid property through the enhanced relative abundance of Asp, Thr, and Glu in polysaccharide-protein fractions. Further purification and electrophoresis identified that the polysaccharide-protein PEG- 1with three subunits possibly was the target cytotoxical component. Our findings proved that mature fruiting body of P. eryngii containing these polysaccharide-proteins possessed highly nutritional values and therapeutical benefits. © 2014 American Chemical Society.


Cui F.,Jiangsu University | Cui F.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production | Zan X.,Jiangsu University | Li Y.,Jiangsu University | And 9 more authors.
International Journal of Biological Macromolecules | Year: 2013

A novel glycoprotein GFG-3a with the molecular weight of 88.01. kDa and potent anti-tumor activity was isolated from the cultured mycelia of Grifola frondosa GF9801. GFG-3a was heat-sensitive with the decreasing anti-proliferative activity after treated from 56. °C to 100. °C for 10-120. min. GFG-3a was a glycoprotein with O-glycosylation and contained 6.20% carbohydrate composed of d-arabinose, d-fructose, d-mannose, and d-glucose with a molar ratio of 1.33:4.51:2.46:1.00. FT-IR and NMR spectra proved that GFG-3a contained protein and carbohydrate portions with 3-O-methyl-galactose residues, (1→4)-linked β-galactose residues, and β-linked glucose residues. Circular dichroism (CD) revealed that GFG-3a was a predominantly β-sheet glycoprotein with a relatively small α-helical content. Protein sequencing and 3D model of GFG-3a were finally predicted by using MALDI-TOF-MS, NCBI blast search and online SWISS-MODLE Workspace service. Our findings will be a reference for the further structure-activity relationship analysis of the mushroom glycoproteins. © 2013 Elsevier B.V.


Sun W.-J.,Jiangsu University | Sun W.-J.,Jiangxi Provincial Engineering and Technology Center for Food Additives Bio production | Sun W.-J.,Parchn Sodium Isovitamin C Co. | Zhao H.-X.,Jiangsu University | And 9 more authors.
Chemistry Central Journal | Year: 2013

Background: Isoascorbic acid is a stereoisomer of L-ascorbic acid, and widely used as a food antioxidant. However, its highly hydrophilic behavior prevents its application in cosmetics or fats and oils-based foods. To overcome this problem, D-isoascorbyl palmitate was synthesized in the present study for improving the isoascorbic acid's oil solubility with an immobilized lipase in organic media. The structural information of synthesized product was clarified using LC-ESI-MS, FT-IR, 1H and 13C NMR analysis, and process parameters for high yield of D-isoascorbyl palmitate were optimized by using One-factor-at-a-time experiments and response surface methodology (RSM).Results: The synthesized product had the purity of 95% and its structural characteristics were confirmed as isoascorbyl palmitate by LC-ESI-MS, FT-IR, 1H, and 13C NMR analysis. Results from " one-factor-at-a-time" experiments indicated that the enzyme load, reaction temperature and D-isoascorbic-to-palmitic acid molar ratio had a significant effect on the D-isoascorbyl palmitate conversion rate. 95.32% of conversion rate was obtained by using response surface methodology (RSM) under the the optimized condition: enzyme load of 20% (w/w), reaction temperature of 53°C and D- isoascorbic-to-palmitic acid molar ratio of 1:4 when the reaction parameters were set as: acetone 20 mL, 40 g/L of molecular sieves content, 200 rpm speed for 24-h reaction time.Conclusion: The findings of this study can become a reference for developing industrial processes for the preparation of isoascorbic acid ester, which might be used in food additives, cosmetic formulations and for the synthesis of other isoascorbic acid derivatives. © 2013 Sun et al.; licensee Chemistry Central Ltd.

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