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Yang H.-Y.,Chinese Institute of Clinical Medical Sciences | Wu X.-M.,Chinese Institute of Clinical Medical Sciences | Liu Y.,Jiangxi Province Peoples Hospital | He D.,Chinese Institute of Clinical Medical Sciences
Transplantation | Year: 2015

Background Chronic ethanol exposure leads to permanent damage to the central nervous system and produces cognitive deficits such as learning and memory impairment. The present study was designed to explore the therapeutic effect of bone marrow mesenchymal stem cells (BMMSCs) on a rat model of alcohol-associated dementia (AAD). Methods Bone marrow mesenchymal stem cells were prelabeled with 4',6-diamidino-2-phenylindole and directly transplanted into the hippocampus of AAD rats, an important site of alcohol effects that lead to cognitive deficits. The therapeutic effect of BMMSCs was evaluated by observing Morris water maze behavior, hippocampus morphology, and neuronal apoptosis. Still, the activities of antioxidant enzymes including total superoxide dismutase and glutathione peroxidase in rat hippocampus were measured, and the expression of brain-derived neurotrophic factor in rat hippocampus was also detected by the method of immunohistochemistry. Results Transplantation of BMMSCs directly into the hippocampus significantly improved the learning and memory function of AAD rats and prevented alcohol-induced hippocampal damages. Moreover, BMMSC transplantation inhibited neuron cell apoptosis and increased the activity of total superoxide dismutase in the hippocampus. Moreover, transplantation of BMMSCs improved the protein level of brain-derived neurotrophic factor in the hippocampus in parallel with behavioral and histologic recovery for AAD rats. Conclusions The findings indicate that the functional benefit observed in the BMMSC-grafted AAD rats is caused by the reduction of oxidative damage and the production of trophic factors by BMMSCs. Bone marrow mesenchymal stem-cell transplantation may be a useful and feasible method for clinical treatment of alcohol-induced brain injuries. © 2015 Wolters Kluwer Health, Inc.

Yang H.-Y.,Institute of Clinical Medicial science | Yang H.-Y.,Peking University | Pu X.-P.,Peking University | Liu Y.,Jiangxi Province Peoples Hospital
Drug and Alcohol Dependence | Year: 2014

Background: It has been shown that opioid dependence-related neuronal plasticity may rely not only on protein synthesis, but also on protein degradation, mainly mediated by ubiquitin-proteasome system (UPS). The aim of the present study was to determine the effect of morphine on the regulation of protein degradation in the brain and to determine which proteins are involved in the underlying mechanism. Methods: Mice were given chronic morphine administration and the state of morphine dependence was confirmed by induction of naloxone-precipitated withdrawal jumping. The level of ubiquitinated proteins in the striatum and spinal cord of morphine-dependent mice was detected by Western blotting. One of the abnormal-ubiquitinated proteins in mice striatum was identified by electrospray ionization quadrupole time-of-flight tandem mass spectrometry and the result was further confirmed by Western blotting and immunofluorescence method. Results: We found that the expression of some ubiquitinated proteins was clearly decreased in the striatum of morphine-depnendent mice, but not in the spinal cord. And we identified a ubiquitinated protein (>79. kDa) decreased in the striatum as heat shock cognate 70 protein, one member of the 70. kDa family of heat shock proteins (HSP70). Moreover, we confirmed the level of HSP70 protein was significantly increased in mice striatum. Conclusions: These data strongly suggest morphine-induced HSP70 overexpression in the striatum is closely related with its abnormal degradation by UPS and it seems to be an important mechanism associated with morphine dependence. © 2014 Elsevier Ireland Ltd.

Zhong Z.-M.,Southern Medical University | Li M.,PLA Fourth Military Medical University | Han Z.-M.,Nanchang University | Zeng J.-H.,Jiangxi Province Peoples Hospital | And 3 more authors.
Clinical Neurology and Neurosurgery | Year: 2016

Objective Dysphagia is a common occurrence after anterior cervical spine surgery. The aim of this meta-analysis was to evaluate the incidence of dysphagia after ervical disc arthroplasty (CDA) compared with anterior cervical discectomy and fusion (ACDF). Material and methods The electronic databases, including PubMed, EMBASE and Cochrane Central Register of Controlled Trials, were searched to identify the randomized controlled trials comparing CDA with ACDF. Studies were included only if the incidence of postoperative dysphagia was investigated. Study selection, "risk of bias" assessment, and data extraction were independently performed by two investigators. Data analyses were conducted with RevMan 5.3 software. Results Ten studies involving 2711 patients (CDA group, n = 1512; ACDF group, n = 1199) were identified. All studies were determined to have a low risk of bias. Pooling analysis of these studies showed that the incidence of dysphagia was 9.46% (143/1512) after CDA versus 12.09% (145/1199) after ACDF. Meta-analysis showed the statistical difference between two groups with regards to the incidence of dysphagia (risk ratio 0.76; 95% confidence interval [0.61, 0.94]; P = 0.01). Conclusion This meta-analysis indicates that patients have a significantly lower incidence of dysphagia after CDA than after ACDF. Additional studies are needed. © 2016 Published by Elsevier B.V.

Liu Y.,Jiangxi Province Peoples Hospital | Yang H.-Y.,Chinese Institute of Clinical Medical Sciences
Chinese Journal of Cancer Prevention and Treatment | Year: 2016

OBJECTIVE: To introduct the latest breast cancer screening guideline of American Cancer Society (ACS) published in 2015. METHODS: We compared the ACS guidelines published in 2015 with which in 2003 to find their differences. Also, we indicated differences between the ACS guideline and other important international breast cancer screening guidelines. In combination with Chinese actual situation, we further discussed the deficiency existing in the ACS guideline and what could be used for us. RESULTS: In the ACS guideline published in 2015, it mainly recommends that women with average risk of breast cancer would routinely receive breast cancer screening by breast photography from the age of 45, and clinical breast examination is not recommended as one of breast cancer screening methods for any age women of average risk. Since there are quite different in breast texture, size and economic conditions for Chinese or European/American female, the screening guideline recommended by ACS is not completely applicable to Chinese female. CONCLUSION: On basis of ACS guideline, we should aim at Chinese female physiology and breast cancer epidemiological characteristics for formulating appropriate guidelines of breast cancer screening in our country. © 2016, Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved.

Jiang Y.,First Peoples Hospital of Shunde | Xiao Q.,Jiangxi Province Peoples Hospital | Hu Z.,Chongqing Medical University | Pu B.,Kunming Medical College | And 4 more authors.
Orthopedics | Year: 2014

The objective of this study was to observe the expression of leukemia inhibitory factor (LIF) in animals and in different clinical grades of patient osteoarthritic tissues. Thirty-five rabbits were used in a Colombo model of experimental osteoarthritis (OA). Five rabbits each were sacrificed on postoperative days 3, 7, 14, 28, 42, 56, and 84. Immunohistochemistry analysis for LIF expression and distribution in the cartilage and synovium of animals was performed at these times. Sixty-seven samples of human articular tissue were obtained from patients with different grades of OA according to symptoms and radiographic inspection. The mRNA expression of LIF was determined by reverse transcription polymerase chain reaction, and LIF protein was determined by enzyme-linked immunosorbent assay (ELISA). The results showed a slight expression of LIF in normal cartilage tissue but less in synovium tissue; however, the expression of LIF was marked in synovial lining cells and superficial and middle-layer cartilage in animal OA (P<.05). Leukemia inhibitory factor mRNA was expressed at the highest level in moderate degrading subchondral bone, and LIF was expressed at the highest level in seriously degrading articular cartilage tissue. These results were similar to those found with ELISA. This study suggests that LIF in OA articular tissues varies by clinical symptoms and grade. It plays an important role in the pathogenesis of OA.

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