Yang H.-Y.,Jiangxi Province Peoples Hospital |
Yang H.-Y.,Peking University |
Pu X.-P.,Peking University |
Liu Y.,Jiangxi Province Peoples Hospital
Drug and Alcohol Dependence | Year: 2014
Background: It has been shown that opioid dependence-related neuronal plasticity may rely not only on protein synthesis, but also on protein degradation, mainly mediated by ubiquitin-proteasome system (UPS). The aim of the present study was to determine the effect of morphine on the regulation of protein degradation in the brain and to determine which proteins are involved in the underlying mechanism. Methods: Mice were given chronic morphine administration and the state of morphine dependence was confirmed by induction of naloxone-precipitated withdrawal jumping. The level of ubiquitinated proteins in the striatum and spinal cord of morphine-dependent mice was detected by Western blotting. One of the abnormal-ubiquitinated proteins in mice striatum was identified by electrospray ionization quadrupole time-of-flight tandem mass spectrometry and the result was further confirmed by Western blotting and immunofluorescence method. Results: We found that the expression of some ubiquitinated proteins was clearly decreased in the striatum of morphine-depnendent mice, but not in the spinal cord. And we identified a ubiquitinated protein (>79. kDa) decreased in the striatum as heat shock cognate 70 protein, one member of the 70. kDa family of heat shock proteins (HSP70). Moreover, we confirmed the level of HSP70 protein was significantly increased in mice striatum. Conclusions: These data strongly suggest morphine-induced HSP70 overexpression in the striatum is closely related with its abnormal degradation by UPS and it seems to be an important mechanism associated with morphine dependence. © 2014 Elsevier Ireland Ltd.
Yang H.-Y.,National University of Singapore |
Yang H.-Y.,JiangXi Province Peoples Hospital |
Wu Z.-Y.,National University of Singapore |
Wood M.,University of Exeter |
And 2 more authors.
Antioxidants and Redox Signaling | Year: 2014
Aims: The best-established mechanism of opioid dependence is the up-regulation of adenylate cyclase (AC)/cAMP pathway, which was reported to be negatively regulated by hydrogen sulfide (H2S), a novel endogenous neuromodulator. The present study was, therefore, designed to determine whether H2S is able to attenuate the development of opioid dependence via down-regulating AC/cAMP pathway. Results: We demonstrated that application of sodium hydrosulphide (NaHS) and GYY4137, two donors of H2S, significantly alleviated naloxone-induced robust withdrawal jumping (the most sensitive and reliable index of opioid physical dependence) in morphine-treated mice. Repeated treatment with NaHS inhibited the up-regulated protein expression of AC in the striatum of morphine-dependent mice. Furthermore, NaHS also attenuated morphine/naloxone-elevated mRNA levels of AC isoform 1 and 8, production of cAMP, and phosphorylation of cAMP response element-binding protein (CREB) in mice striatum. These effects were mimicked by the application of exogenous H2S or over-expression of cystathione-β-synthase, an H2S -producing enzyme, in SH-SY5Y neuronal cells on treatment with [D-Ala2,N-Me-Phe4,Gly5-ol]-Enkephalin, a selective μ-opioid receptor agonist. Blockade of extracellular-regulated protein kinase 1/2 (ERK1/2) with its specific inhibitor attenuated naloxone-induced CREB phosphorylation. Pretreatment with NaHS or stimulation of endogenous H2S production also significantly suppressed opioid withdrawal-induced ERK1/2 activation in mice striatum or SH-SY5Y cells. Innovation: H2S treatment is important in prevention of the development of opioid dependence via suppression of cAMP pathway in both animal and cellular models. Conclusion: Our data suggest a potential role of H 2S in attenuating the development of opioid dependence, and the underlying mechanism is closely related to the inhibition of AC/cAMP pathway. Antioxid. Redox Signal. 20, 31-41. © Copyright 2014, Mary Ann Liebert, Inc. 2014.
Effect of Post-procedural Full Does Infusion of Bivalirudin on Acute Stent Thrombosis in Patients with ST-elevation Myocardial Infarction Undergoing Primary Percutaneous Coronary Intervention: Outcomes in a large real-world population
PubMed | Xinjiang Corps General Hospital, Tianjin Chest Hospital, Dongying Peoples Hospital, Shenzhen Sun Yat sen Cardiovascular Hospital and 8 more.
Type: | Journal: Cardiovascular therapeutics | Year: 2017
The current study aimed to evaluate the effect of prolonged full-does bivalirudin infusion in real-world population with ST-elevation Myocardial Infarction(STEMI).Subgroup data as well as meta analysis from randomized clinical trials have showed potency of post-procedural full dose infusion (1.75mg/kg/h) of bivalirudin on attenuating acute stent thrombosis(ST) after primary percutaneous coronary intervention(PCI).In this multicenter retrospective observational study, 2047 consecutive STEMI patients treated with bivalirduin during primary PCI were enrolled in 65 Chinese centers between July 2013 and May 2016. The primary outcome was acute ST defined as ARC definite/probable within 24 hours after the index procedure, and the secondary endpoints included total ST, major adverse cardiac or cerebral events (MACCE, defined as death, re-infarction, stroke and target vessel revascularization) and all bleeding at 30 days.Among 2047 STEMI patients, 1123 (54.9%) were treated with post-procedrual bivalirudin full dose infusion (median 120 min)while the other 924 (45.1%) received low does (0.25mg/kg/h) or null post-procedural infusion. A total of 3 acute ST (0.3%) occurred in STEMI patients with none or low does prolonged infusion of bivairudin, but none was observed in those treated with post-PCI full does infusion(0.3% vs 0.0%, P=0.092). Outcomes on MACCE (2.1% vs. 2.7%, p=0.402), total bleeding (2.1% vs. 1.4%, p=0.217) at 30 days showed no significant difference between the two groups and no subacute ST was observed.Post-PCI full-does bivalirudin infusion is safe and has a trend to protect against acute ST in STEMI patients undergoing primary PCI in real world settings. This article is protected by copyright. All rights reserved.
Jiang M.,Nanchang University |
Jiang M.,Jiangxi Province Peoples Hospital |
Zhang P.,Nanchang University |
Hu G.,Jiangxi Province Peoples Hospital |
And 6 more authors.
Molecular and Cellular Biochemistry | Year: 2013
Objective was to assess and compare the relative expressions of miR-205-5p, miR-205-3p, and miR-21-3p in tissues and serum among non-small cell lung carcinoma (NSCLC) patients, benign pulmonary conditions patients, and healthy volunteers. Serum samples were obtained between October 2011 and September 2012 from 20 NSCLC patients undergoing surgical treatment, 20 patients diagnosed with a benign lung disease (pulmonary tuberculosis, pneumonia, chronic obstructive pulmonary disease, or interstitial pneumonia) (lesion group), and 20 healthy volunteers (control group). NSCLC patients provided cancer tissues and cancer-adjacent normal tissues during surgery (paired specimens). Quantitative RT-PCR was used to assess miR-205-5p, miR-205-3p, and miR-21-3p expressions in serum and tissue samples. The relative expressions of miR-205-5p and miR-205-3p were significantly higher in NSCLC tissues compared with cancer-adjacent paired specimens (both P < 0.001). In the serum, significantly higher miR-205-5p, miR-205-3p, and miR-21-3p relative expressions were observed in the NSCLC group compared with the two other groups (all P < 0.001). The relative expressions of miR-205-5p and miR-21-3p in NSCLC tissues and serum were significantly correlated (r = 0.553, P = 0.011; and r = -0.541, P = 0.014, respectively), while no significant correlation was observed for miR-205-3P (P = 0.120). Expressions of miR-205-5p and miR-205-3P in squamous cell carcinoma specimens were significantly higher than in lung adenocarcinoma specimens (both P = 0.001). Similarly, higher serum miR-205-5p and miR-205-3p levels were observed in squamous cell carcinoma patients (P = 0.033 and P = 0.002, respectively). In this preliminary and novel study, miR-205-5p was more useful as a marker for NSCLC than miR-205-3p or miR-21, indicating a potential for future applications in NSCLC diagnosis and prognosis. © 2013 Springer Science+Business Media New York.
PubMed | Nanchang University, Jiangxi Province Peoples Hospital and Nanchang Reproductive Hospital
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016
The objective of this study was to identify disrupted pathways in teratozoospermia by systematically tracking dysregulated modules in reweighted protein-protein interaction (PPI) networks. We inferred and reweighted the PPI networks of normal and teratozoospermia groups based on Spearman correlation coefficients. Modules in the PPI networks were explored via a clique-merging algorithm and altered modules were identified based on maximum weight bipartite matching. Furthermore, pathway-enrichment analyses of genes in altered modules were performed by Database for Annotation, Visualization, and Integrated Discovery (DAVID) to illuminate the biological pathways in teratozoospermia. A total of 20,102 genes were screened from the expression profile. We explored 2406 and 2101 modules in normal and disease PPI networks, respectively. Moreover, we obtained 875 altered modules by comparing modules in normal and teratozoospermia PPI networks. At P < 0.01, the genes involved in 2855 interactions with score changes >1 were mainly enriched in 66 pathways and the genes in altered modules were enriched in 71 pathways. The activity genes (missed and added genes in the disrupted modules) were enriched in 41 common pathways. There were 36 mutual enriched pathways under the five different conditions. Moreover, the cell cycle pathway was disrupted in the first 10 pathways of each condition. This study provides a powerful biomarker discovery platform to better understand the progression of teratozoospermia by systematically tracking dysregulated modules. This method uncovered potential diagnostic and therapeutic targets of teratozoospermia. This information might lead to improved monitoring and treatment of teratozoospermia.
Xu M.,Jiangxi Province Peoples Hospital |
Zhang Q.,Guangzhou University |
Qiu H.,Qingdao Stomatological Hospital
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology | Year: 2013
Loss of anterior tooth impairs patients physically and emotionally as well as presents a challenge for dentists. This article presented a method of replacing one anterior tooth that was extracted because of periodontitis. The proposed method involves the use of the natural crown as pontic with fiber-reinforced composite resin-bonded fixed partial denture.
Liu Y.,Jiangxi Province Peoples Hospital |
Yang H.-Y.,Chinese Institute of Clinical Medical Sciences
Chinese Journal of Cancer Prevention and Treatment | Year: 2016
OBJECTIVE: To introduct the latest breast cancer screening guideline of American Cancer Society (ACS) published in 2015. METHODS: We compared the ACS guidelines published in 2015 with which in 2003 to find their differences. Also, we indicated differences between the ACS guideline and other important international breast cancer screening guidelines. In combination with Chinese actual situation, we further discussed the deficiency existing in the ACS guideline and what could be used for us. RESULTS: In the ACS guideline published in 2015, it mainly recommends that women with average risk of breast cancer would routinely receive breast cancer screening by breast photography from the age of 45, and clinical breast examination is not recommended as one of breast cancer screening methods for any age women of average risk. Since there are quite different in breast texture, size and economic conditions for Chinese or European/American female, the screening guideline recommended by ACS is not completely applicable to Chinese female. CONCLUSION: On basis of ACS guideline, we should aim at Chinese female physiology and breast cancer epidemiological characteristics for formulating appropriate guidelines of breast cancer screening in our country. © 2016, Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved.
Yang H.-Y.,Chinese Institute of Clinical Medical Sciences |
Wu X.-M.,Chinese Institute of Clinical Medical Sciences |
Liu Y.,Jiangxi Province Peoples Hospital |
He D.,Chinese Institute of Clinical Medical Sciences
Transplantation | Year: 2015
Background Chronic ethanol exposure leads to permanent damage to the central nervous system and produces cognitive deficits such as learning and memory impairment. The present study was designed to explore the therapeutic effect of bone marrow mesenchymal stem cells (BMMSCs) on a rat model of alcohol-associated dementia (AAD). Methods Bone marrow mesenchymal stem cells were prelabeled with 4',6-diamidino-2-phenylindole and directly transplanted into the hippocampus of AAD rats, an important site of alcohol effects that lead to cognitive deficits. The therapeutic effect of BMMSCs was evaluated by observing Morris water maze behavior, hippocampus morphology, and neuronal apoptosis. Still, the activities of antioxidant enzymes including total superoxide dismutase and glutathione peroxidase in rat hippocampus were measured, and the expression of brain-derived neurotrophic factor in rat hippocampus was also detected by the method of immunohistochemistry. Results Transplantation of BMMSCs directly into the hippocampus significantly improved the learning and memory function of AAD rats and prevented alcohol-induced hippocampal damages. Moreover, BMMSC transplantation inhibited neuron cell apoptosis and increased the activity of total superoxide dismutase in the hippocampus. Moreover, transplantation of BMMSCs improved the protein level of brain-derived neurotrophic factor in the hippocampus in parallel with behavioral and histologic recovery for AAD rats. Conclusions The findings indicate that the functional benefit observed in the BMMSC-grafted AAD rats is caused by the reduction of oxidative damage and the production of trophic factors by BMMSCs. Bone marrow mesenchymal stem-cell transplantation may be a useful and feasible method for clinical treatment of alcohol-induced brain injuries. © 2015 Wolters Kluwer Health, Inc.
Chen Z.Q.,Jiangxi Province Peoples Hospital
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2011
To observe the effect of Tongxinluo Capsule on platelet activities and vascular endothelial functions as well as prognosis in patients with acute coronary syndrome (ACS) after percutaneous coronary intervention (PCI) at different stages. 160 patients with acute coronary syndrome were randomly assigned to Tongxinluo (TXL) group (80 patients) and the conventional treatment group (80 patients). And 50 healthy subjects were set up as the health control group. Patients' plasma platelet activating factors (CD62p, CD63), and glucose protein (GP) IIb/IIIa, and endothelium-1 (ET-1), von Willebrand factor (vWF), nitric oxide (NO) levels, and endothelium dependent flow-mediated dilatation (FMD) were detected respectively. Patients in the TXL group orally took TXLC for six months. The aforesaid indices were re-detected in all patients after two months and six months. Comparison between before and after treatment in the same group and inter-group comparison were performed in the two groups. Compared with the health control group, CD62p, CD63, GPIIb/IIIa, vWF, and ET-1 levels increased significantly in ACS patients after PCI (all P<0.01), NO and FMD significantly decreased (P<0.01). CD62p, CD63, GPIIb/IIIa and, vWF also increased, and FMD decreased after PCI (all P<0.05), but insignificant difference was found in ET-1 and NO (P>0.05). In the TXL group and the conventional treatment group, the levels of CD62p, CD63, GPIIb/IIIa, vWF and ET-1 decreased significantly (P<0.05, P<0.01), NO and FMD increased (P<0.05, P<0. 01) when compared with before treatment. Compared with the conventional treatment group, the decrement of CD62p, CD63, GPIIb/IIIa and vWF (P<0.05, P<0.01), and the increment of FMD and NO (both P<0.05) were more obvious in the TXL group. The aforesaid indices were more obviously different between 6-month treatment and 2-month treatment in the TXL group and the conventional treatment group (P<0.05, P<0.01). Seven patients suffered from angina, six from heart failure, three from ventricular tachycardiac (VT)/ventricular fibrillation (VF), and two died suddenly in the conventional treatment group after six months of treatment, while only one suffered from angina, one from heart failure, and none from VT/VF or died suddenly in the TXL treatment group after 6 months of treatment. TXL could be used in the prevention and treatment of coronary thrombosis, protect the vascular endothelial functions, as well as improve the prognosis of ACS patients after PCI.
Liu H.-Y.,Jiangxi Province Peoples Hospital |
Liu T.-T.,Jiangxi Province Peoples Hospital
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology | Year: 2015
Aplastic anemia is a disease characterized by low bone marrow hematopoietic function and derease of whole blood cells caused by a variety of reasons. Its pathogenesis includes abnormality of hematopoietic stem cells (seed theory), hematopoietic microenvironment (soil theory) and immune function (such as worms theory). These 3 causes of disease interact each other and facilitate the development of aplastic anemia, thereby increase the complexity of the etiological diagnosis and uncertainty of treatment. On this basis, this review summarizes the latest research progress on the blood supply of bone marrow microcirculation in the hematopoietic microenvironment, stromal cells, cytokins, the immune function of dendritic cells, natural killer cells, T cell subgroup, the secretion of cytokines, cell signal transduction, and hematopoietic stem cell gene abnormality to provides the theoretic basis for the diagnosis and treatment of aplastic anemia.