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Yu X.,Jiangxi Maternity and Child Healthcare Hospital
International Journal of Gynecological Cancer | Year: 2013

Objectives: DJ-1 was originally cloned as a putative oncogene capable of transforming NIH3T3 cells in cooperation with H-Ras or c-Myc, which has been implicated in the pathogenesis of some solid tumors. The aim of this study was to investigate the expression and clinical significance of DJ-1 in endometrial cancer and study its effect on cell proliferation and apoptosis in endometrial cancer Ishikawa cells. Methods: Reverse transcription polymerase chain reaction and Western blotting were performed to determine the DJ-1 expression in 100 surgical specimens of endometrial cancer tissues, paired tumor-adjacent tissues, and 30 surgical specimens of normal endometrium tissues. The proliferation variety of endometrial cancer Ishikawa cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay after transfecting the interference plasmid pGPU6/GFP/neo-DJ-1-shRNA into Ishikawa cells. Real-time polymerase chain reaction and Western blotting were used to evaluate the effect of interference plasmid on target gene expression. Apoptosis rate was determined by flow cytometry. Results: DJ-1 expression in endometrial cancer tissues was higher than in tumor-adjacent tissues and normal endometrial tissues. At the same time, it was associated with signs of cancer progression, including differentiation, myometrial invasion depth, and presence of lymph node metastasis. Knocking down DJ-1 promoted the apoptosis of Ishikawa cells. Conclusions: High DJ-1 expression seems to be negatively correlated with apoptosis. Meanwhile, it may be part of the mechanisms for the development, invasion, and metastasis in endometrial cancer. © 2013 by IGCS and ESGO. Source


Guo J.,Nanchang University | Gao J.,Jiangxi Maternity and Child Healthcare Hospital | Yu X.,Jiangxi Maternity and Child Healthcare Hospital | Luo H.,Jiangxi Maternity and Child Healthcare Hospital | And 2 more authors.
Gynecologic and Obstetric Investigation | Year: 2015

Objective: Endometrial cells may aberrantly express molecules involved in invasion and migration, leading to endometriosis. The aim of this study was to investigate the expression of DJ-1 and phosphorylated mammalian target of rapamycin (p-mTOR) in ectopic and eutopic endometria of endometriosis and adenomyosis. Methods: Endometrial specimens were obtained from healthy non-menopausal women (n = 17) or patients with ovarian endometriotic cysts (n = 48) or adenomyosis (n = 30) during January 2011 to June 2012. The expressions of DJ-1 and p-mTOR were evaluated by immunohistochemistry and western blotting methods. Results: The expressions of DJ-1 and p-mTOR were significantly higher in the ectopic endometria than those in the eutopic endometria of endometriosis and adenomyosis patients or normal endometria (FDR < 0.05). DJ-1 expression was positively correlated with the p-mTOR expression no matter at endometriosis (r = 0.736, FDR < 0.001) or adenomyosis (r = 0.809, FDR < 0.001). Conclusion: DJ-1 protein may be involved in endometrial cells proliferation, migration and angiogenesis by modulating the PI3K/Akt/p-mTOR signaling pathway, which provides an underlying theoretical target for endometriosis and adenomyosis. © 2015 S. Karger AG, Basel. Source


Zhang Z.-Q.,Jiangxi Maternity and Child Healthcare Hospital | Long S.-G.,Jiangxi Maternity and Child Healthcare Hospital | Huang Z.-H.,Jiangxi Maternity and Child Healthcare Hospital | Xin C.-L.,Jiangxi Maternity and Child Healthcare Hospital | Wu Q.-F.,Jiangxi Maternity and Child Healthcare Hospital
Andrologia | Year: 2016

Different outcomes after intracytoplasmic sperm injection (ICSI) without oocyte activation in two patients with different types of round-headed spermatozoa (globozoospermia) are reported. After controlled ovarian hyperstimulation and oocyte pick-up, retrieved oocytes were underwent ICSI without oocyte activation and a 33.33% (4/12) fertilisation rate was obtained in the first case, whereas an abnormal fertilisation was achieved in the second case. The transfer of two grade II embryos in the first couple resulted in clinical pregnancy with a healthy livebirth. It was concluded that the main problem of cases with globozoospermia was a low fertilisation rate or failure fertilisation, and even though ICSI and artificial oocyte activation have been employed to increase this rate, it is not necessarily needed to achieve a pregnancy. © 2016 Blackwell Verlag GmbH. Source


Xiao Z.-Q.,Jiangxi Maternity and Child Healthcare Hospital | Long S.-G.,Jiangxi Maternity and Child Healthcare Hospital | Zhu Q.-Z.,Jiangxi Maternity and Child Healthcare Hospital | Yan J.-J.,Jiangxi Maternity and Child Healthcare Hospital | And 2 more authors.
Tumor | Year: 2014

Objective: To investigate the effects of DJ-1 gene silencing on proliferation and apoptosis of endometrial cancer Ishikawa cells. Methods: After the specific interference plasmid pGPU6/GFP/neo-DJ-1-small hairpin RNA (shRNA) targeting DJ-1 gene was transfected into Ishikawa cells, the change of cell proliferation was detected by MTT method, the expressions of DJ-1 mRNA and protein were detected by real-time fluorescence quantitative PCR and Western blotting, as well as the apoptosis of Ishikawa cells was detected by Annexin-V/propidium iodide (PI) dual staining and flow cytometry, respectively. Results: The transfection of pGPU6/GFP/neo-DJ-1-shRNA significantly inhibited the proliferation of Ishikawa cells in a dose- and time-dependent manner (P < 0.05). The expressions of DJ-1 mRNA and protein in Ishikawa cells transfected with pGPU6/GFP/neo-DJ-1-shRNA were significantly lower than those in the control groups (P < 0.01). The apoptosis rate in pGPU6/GFP/neo-DJ-1-shRNA transfection group was significantly higher than that of the control groups (P < 0.01). Conclusion: DJ-1 gene silencing can inhibit the proliferation of endometrial cancer Ishikawa cells and promote the apoptosis. Copyright© 2014 by TUMOR. Source

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