Lin D.-Q.,Jiangxi Institute of Supervision and Inspection on Product Quality |
Wan C.-B.,Jiangxi Institute of Supervision and Inspection on Product Quality |
Qiu P.,Nanchang University |
Liu H.-M.,Jiangxi Institute of Supervision and Inspection on Product Quality
Journal of Chinese Mass Spectrometry Society | Year: 2013
A method based on high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was developed for rapid determination of the residues of four yellow industrial dyes (basic orange II, auramine O, orange acid II and metal yellow) in foods. The sample were extracted by acetonitrile, and then analyzed in multiple reaction monitoring (MRM) mode. Sample matrix-matched calibration was used to determine the residue contents by external standard. Basic orange II and auramine O were detected with positive ion model, while orange acid II, metal yellow were detected with negative ion model. The mobile phase was ultrapure water (containing 0.1% formic acid for positive ion model)-acetonitrile. Under the optimized detection conditions, the linear ranges for basic orange II and auramine O are 5.0-200.0 μg/L, and for orange acid II and metal yellow are 5.0-100.0 μg/L, the linear correlation coefficients are all more than 0.999. The limits of quantification of basic orange II, auramine O, orange acid II and metal yellow are 50, 50, 75, 60 μg/kg, respectively. The recoveries of 4 dyes range from 75.6% to 119.0% with relative standard deviations (RSD) of 3.1%-9.1%. This method is simple, effective, sensitive, which is suitable for the determination and confirmation of four industrial dyes added in the foods illegally.