Lin D.-Q.,Jiangxi Institute of Supervision and Inspection on Product Quality |
Wan C.-B.,Jiangxi Institute of Supervision and Inspection on Product Quality |
Qiu P.,Nanchang University |
Liu H.-M.,Jiangxi Institute of Supervision and Inspection on Product Quality
Journal of Chinese Mass Spectrometry Society | Year: 2013
A method based on high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was developed for rapid determination of the residues of four yellow industrial dyes (basic orange II, auramine O, orange acid II and metal yellow) in foods. The sample were extracted by acetonitrile, and then analyzed in multiple reaction monitoring (MRM) mode. Sample matrix-matched calibration was used to determine the residue contents by external standard. Basic orange II and auramine O were detected with positive ion model, while orange acid II, metal yellow were detected with negative ion model. The mobile phase was ultrapure water (containing 0.1% formic acid for positive ion model)-acetonitrile. Under the optimized detection conditions, the linear ranges for basic orange II and auramine O are 5.0-200.0 μg/L, and for orange acid II and metal yellow are 5.0-100.0 μg/L, the linear correlation coefficients are all more than 0.999. The limits of quantification of basic orange II, auramine O, orange acid II and metal yellow are 50, 50, 75, 60 μg/kg, respectively. The recoveries of 4 dyes range from 75.6% to 119.0% with relative standard deviations (RSD) of 3.1%-9.1%. This method is simple, effective, sensitive, which is suitable for the determination and confirmation of four industrial dyes added in the foods illegally.
Kuang H.,Nanchang University |
Gan B.,Jiangxi Institute of Supervision and Inspection on Product Quality |
Guo L.,Nanchang University |
Aguilar Z.P.,Ocean NanoTech, LLC |
Xu H.,Nanchang University
Analytical Letters | Year: 2016
Sulfo-functionalized iron oxide magnetic nanobeads were employed as an adsorbent for magnetic solid-phase extraction of benzodiazepines in beef. The analytes were determined by high performance liquid chromatography–mass spectrometry. The sulfo-functionalized iron oxide magnetic nanobeads were characterized by transmission electron microscopy and infrared spectroscopy. The optimal pH, ionic strength, volume of sulfo-functionalized iron oxide magnetic nanobeads, and elution solvent were investigated for the isolation procedure. Under the optimized conditions, the limits of detection for diazepam, oxzepam, triazolam, and chlordiazepoxide were 0.79, 0.22, 0.13, and 0.23 ng/kg, respectively. The method was employed for the determination of the analytes in beef. Compared with established methods, magnetic solid phase extraction and high-performance liquid chromatography–tandem mass spectrometry method exhibited high sensitivity, rapid separation, and low cost. © 2016, Copyright © Taylor & Francis Group, LLC.