Jiangxi Fisheries Research Institute

Nanchang, China

Jiangxi Fisheries Research Institute

Nanchang, China
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Ding L.-Y.,Ningbo University | Ding L.-Y.,Jiangxi Fisheries Research Institute | Jin M.,Ningbo University | Sun P.,Ningbo University | And 5 more authors.
Aquaculture | Year: 2017

In the present study, the cDNA of fatty acid-binding protein (Pt-FABP1) from the hepatopancreas of swimming crab (Portunus trituberculatus) was cloned by rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of Pt-fabp1 was 730 bp and encoded a 130 amino acids polypeptide that was highly homologous to related genes from other crustacean. Quantitative PCR analysis revealed that Pt-fabp1 expression was detected in various tissues, and the highest expression levels was occurred in the hepatopancreas. The Pt-fabp1 expression levels in the ovary would depend on the stages of ovarian development, and the expression of Pt-fabp1 reached the maximum at the stage IV. The effects of dietary soybean lecithin (SL) on the Pt-fabp1 and vitellogenin (VTG) expression, and steroid hormones levels were investigated. After 16 weeks of feeding, the results showed that the expression level of Pt-fabp1 in hepatopancreas and the content of progesterone (PG) in hemolymph did significantly increase with dietary SL supplementation up to 2% (P < 0.05). Swimming crab fed the diet containing 4% SL had higher hepatopancreas vtg gene expression and the content of 17 β-estradiol (E2) in hemolymph than those fed the control diet (P < 0.05). This study suggested that Pt-FABP may have a positive function in ovary maturation of female swimming crab. Meanwhile, these results not only demonstrated that the gene expression of Pt-fabp1 and vtg in hepatopancreas can be modulated by dietary SL levels, but also clearly supported the hypothesis that the positive effects of dietary SL on the ovarian development of female swimming crab. © 2017 Elsevier B.V.

Ding L.,Ningbo University | Ding L.,Jiangxi Fisheries Research Institute | Fu H.,Jiangxi Fisheries Research Institute | Hou Y.,Ningbo University | And 3 more authors.
Fisheries Science | Year: 2017

Portunus trituberculatus broodstock were stocked in plastic tanks to evaluate the effects of starvation and feeding on gonadal development, blood chemistry, fatty acid composition, and expression of vitellogenin (Vtg) and fatty acid-binding protein genes (FABP) in females. Two treatments (starved and fed) were randomly assigned to triplicate groups of 90 swimming crab broodstock (approximately 230 ± 45 g). In the starved treatment, crabs were starved for 30 days, whereas in the fed treatment crabs were fed once a day with clams. The gonadosomatic index decreased significantly in starved crabs (P < 0.05), as did the serum glucose and cholesterol concentrations; conversely, the total protein concentration in serum significantly increased (P < 0.05). In the ovary, there was a significant relative decline of 18:0, 16:1n-7 and 20:1n-9 fatty acids and relative increases of 20:4n-6, 22:6n-3, 18:1n-9 and 20:5n-3 in starved crabs compared to fed crabs (P < 0.05). Relative expression of Vtg in the ovary decreased significantly in starved crabs (P < 0.05), while there was no significant difference in hepatopancreas Vtg expression between starved and fed crabs (P > 0.05). Starvation suppressed gonadal development in female swimming crab broodstock. © 2017 Japanese Society of Fisheries Science

Ruan J.,Shanghai Ocean University | Ruan J.,Jiangxi Agricultural University | Hu K.,Shanghai Ocean University | Zhang H.,Shanghai Ocean University | And 5 more authors.
Fish Physiology and Biochemistry | Year: 2014

Gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in brain, is synthesized from glutamate and metabolized to succinic semialdehyde by glutamic acid decarboxylase (GAD) and GABA transaminase (GABA-T), respectively. The fast inhibitory effect of GABA is mediated by GABA type A (GABAA) receptors that are associated with several neurological disorders, and GABAA receptors are targets of several therapeutic agents. To date, information on the distribution and quantity of GABAA receptors in Carassius auratus gibelio is still limited. We investigated for the first time, the tissue-specific distribution of GABAARβ2a and GABAARβ2b, the two subunits of the predominant GABAA receptor subtype (α1β2γ2), and then, the expression of GABAARβ2a, GABAARβ2b, GAD, and quantified GABA-T genes in different tissues by quantitative real-time PCR method and compared different expressions between two developmental stages of C. auratus gibelio. Results showed that GABAARβ2a and GABAARβ2b genes expressed in both brain and peripheral organs using reverse transcription-polymerase chain reaction. In addition, the majority of GABAARβ2a, GABAARβ2b, GAD, and GABA-T were mainly synthesized in brain; however, a considerable amount of GABA-T was secreted from the peripheral tissues, especially in the liver. Moreover, the expression of GABAARβ2a and GABAARβ2b genes in different tissues varied with body weight change. This study provides a reference for further studies on GABA and GABAA receptors subunits and an insight on the possible pharmacological properties of the GABAA receptor in C. auratus gibelio. © 2014 Springer Science+Business Media Dordrecht.

Zhang Y.,Nanjing Agricultural University | Zhang Y.,Jiangxi Fisheries Research Institute | Jiang S.,Chinese Academy of Fishery Sciences | Xiong Y.,Chinese Academy of Fishery Sciences | And 6 more authors.
Turkish Journal of Fisheries and Aquatic Sciences | Year: 2013

The extra sex combs (Esc) gene first identified in Drosophia encodes a transcriptional repressor of homeotic genes belonging to the polycomb group (PcG) gene and is primarily expressed in the early embryos. In this study, we have isolated the full-length cDNA of an Esc gene from the testis of oriental river prawn (Macmbrachium nipponense) according to the established expressed sequence tags (ESTs) information using Rapid Amplification of the cDNA Ends (RACE) technique, designated as MnEsc. The full-length cDNA of MnEsc is 1,461bp in size and has an open reading frame (ORF) of 1,068bp, encoding a 355-amino acid protein. Real-time quantitative PCR analyses revealed that the expression level of MnEsc varied significantly in the developing embryo, postembryonic larval and adult tissues. Real-time quantitative PCR showed the MnEsc gene was expressed in testis, liver, ovary, brain, abdominal ganglion, heart, intestine and muscle with the highest level of expression in ovary and brain. In vivo silencing of the gene, the dsRNA of MnEsc caused a significantly decrease in target gene expression level in brain and ovary tissues, but no exterior appearance change of experimental shrimp was observed. © Published by Central Fisheries Research Institute (CFRI) Trabzon, Turkey in cooperation with Japan International Cooperation Agency (JICA), Japan.

Cao H.,Shanghai Ocean University | Zhang H.,Shanghai Ocean University | Zhang H.,Jiangxi Fisheries Research Institute | He S.,Shanghai Ocean University | And 2 more authors.
Israeli Journal of Aquaculture - Bamidgeh | Year: 2013

Aeromonasis has seriously affected the farming of gibel carp Carassius auratus gibelio, causing a significant and progressive increase in the use of difloxacin. In the present study, a single dose of 20 mg difloxacin per kg body weight was orally administered to healthy and aeromonasis-induced juvenile gibel carp and the pharmacokinetics is described by a two-compartment open model with first-order rate absorption. Compared to the healthy control fish, the absorption, distribution, and elimination rates of difloxacin were greatly reduced and the plasma protein binding level was significantly increased in diseased fish. In addition, as a surrogate marker of antibacterial efficacy, the ratio of maximum plasma concentration (Cmax) to minimum inhibitory concentration (MIC) of difloxacin was calculated; the Cmax/MIC ratios ranged 3.77-18.14 in healthy fish and 1.60-7.70 in diseased fish, indicating that difloxacin at 20 mg/kg body weight cannot ensure successful treatment in aeromonasis-infected fish.

Zhang Y.,Nanjing Agricultural University | Zhang Y.,Jiangxi Fisheries Research Institute | Fu H.,Nanjing Agricultural University | Fu H.,Chinese Academy of Fishery Sciences | And 6 more authors.
Journal of the World Aquaculture Society | Year: 2013

The transformer-2 gene (tra-2) plays a key role in the sexual differentiation regulatory hierarchy. In this study, tra-2 gene homologs designated as Mntra-2 was cloned and characterized from Macrobrachium nipponense. The full-length cDNA of Mntra-2 consists of 1724 bp with an open reading frame (ORF) encoding 192 amino acids, an 827bp 5′-untranslated region (UTR) and a 318bp 3′-UTR. The predicated molecular mass of Mntra-2 was 20.805 kDa with an estimated theoretical isoelectric point of 10.36. The deduced amino acid sequence shares high homology with Penaeus monodon. Real-time quantitative polymerase chain reaction (RT-qPCR) analyses demonstrated that the expression levels of Mntra-2 varied significantly during different developmental stages of embryogenesis, larvae, and post-larvae and in various adult tissues. During embryogenesis, the expression level of Mntra-2 was slightly higher at the cleavage stage than at the blastula stage, and reached the highest level at the nauplius stage. During the larvae, the Mntra-2 expression gradually increased from 1 d larvae post hatch (L1) to L10 and decreased to a lowest level at the end of metamorphosis. During the post-larvae, the Mntra-2 expression was higher level at the 5 d after the metamorphosis (P5). RT-qPCR showed the Mntra-2 mRNA was expressed in ovary, testis, muscle, heart, abdominal ganglion, brain, and intestine with the highest level of expression in muscle and intestine. The results indicate that Mntra-2 is an arthropods tra-2 homolog and probably plays important roles in embryonic development and sex differentiation of M. nipponense. © Copyright by the World Aquaculture Society 2013.

Zhang Y.P.,Nanjing Agricultural University | Zhang Y.P.,Jiangxi Fisheries Research Institute | Qiao H.,Chinese Academy of Fishery Sciences | Zhang W.Y.,Nanjing Agricultural University | And 7 more authors.
Genetics and Molecular Research | Year: 2013

In this study, two Sxl gene homologs, designated as Mnsxl1 and Mnsxl2, were cloned and characterized from the freshwater prawn Macrobrachium nipponense by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnsxl1 and Mnsxl2 showed high sequence homology to the insect Sxl and contained conserved domains in two RNA-binding motifs. Real-time quantitative reverse transcription-polymerase chain reaction (RT-QPCR) showed that the Mnsxl1 and Mnsxl2 genes were expressed in all investigated tissues, with the highest level of expression in the intestine and liver. RT-QPCR also revealed that Mnsxl1 and Mnsxl2 mRNAs expressions were both significantly increased at 5 and 20 days post-larvae after metamorphosis. Thus, the results of the present study imply that Mnsxl1 and Mnsxl2 play complex and important roles in the sex differentiation of M. nipponense. © FUNPEC-RP.

Hu M.,Nanchang University | Zhou H.,Jiangxi Fisheries Research Institute | Wu Z.,Guilin University of Technology | Ouyang S.,Nanchang University | Chen C.,Jinggangshan University
Eco.mont | Year: 2014

Jinggangshan National Nature Reserve is located in the middle reach of the Luoxiao Mountains in the south-western Jiangxi Province. The fish fauna of mountain streams in the nature reserve was investigated seasonally during 2011. A total of 553 samples were collected and classified into 12 families and 38 species. The samples collected in the nature reserve did not include any exotic species. The most abundant fish species collected was Pseudogastromyzon changtingensis tungpeiensis, followed by Acrossocheilus parallens, Rhinogobius cliffordpopei and Rhinogobius giurinus. A total of 16 species were found to be endemic to China. Current threats to the conservation of fishes in the nature reserve were reviewed and management solutions were suggested.

Hu M.,Nanchang University | Hua Q.,Jiangxi Aquaculture Technology Extending Stations | Zhou H.,Jiangxi Fisheries Research Institute | Wu Z.,Guilin University of Technology | Wu X.,Nanchang University
Environmental Biology of Fishes | Year: 2015

The four species of carp present in China (grass carp, black carp, silver carp and bighead carp) are all commercially important freshwater fish species. However, the construction of large-scale hydraulic projects, such as dams, along with other anthropogenic changes to river systems has resulted in the rapid decline in abundance of all four carp species. Indeed, the effect of dams on the reproduction of the four species has become a highly sensitive issue in China. Historically, the Ganjiang River contained 12 spawning grounds for the four species. After the construction of the Wan’an Dam, however, four of these spawning areas were flooded. Three additional dams are currently under construction below the Wan’an Dam. While it is clear that these dams will further alter the hydrological regime in the river, there is currently little information available for the management and protection of carp in this river. In this study, we review published studies to characterize the patterns of larval abundance of the four species of carp in relation to environmental correlates in the Ganjiang River to provide baseline information that can be used to conserve these species. © 2014, The Author(s).

Hu M.-L.,Nanchang University | Zhou H.-M.,Jiangxi Fisheries Research Institute | Li F.,Jiangxi Entry Exit Inspection and Quarantine Bureau
Hydraulic Engineering - Proceedings of the 2012 SREE Conference on Hydraulic Engineering, CHE 2012 and 2nd SREE Workshop on Environment and Safety Engineering, WESE 2012 | Year: 2013

Spatiotemporal variation of Chlorophyll a (Chla) concentration and its relationship with other water quality factors in the Poyang Lake outlet were analyzed during the period of 2007 to 2008. The results indicated that the Chla concentration was 4.46 ±1.53 mg · m-3 in 2007 and 4.60 ±1.67 mg · m-3 in 2008. Additionally, the Chla concentration reached a peak in August or September while bottomed out in January or December. And its values were significantly higher in the left sampling site than that in the middle and right sampling sites. Moreover, the Chla concentration in Poyang Lake outlet showed significant positive correlation with Z, WT, SD while SS, DO, COD and nutrients were inversely related. But no obvious correlation was found between pH and Chla in Poyang Lake outlet. The study would highlight further research needs for the distribution patterns of chlorophyll a in association with hydrological features in Poyang Lake. © 2013 Taylor & Francis Group.

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