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Cao H.,Shanghai Ocean University | Zhang H.,Shanghai Ocean University | Zhang H.,Jiangxi Fisheries Research Institute | He S.,Shanghai Ocean University | And 2 more authors.
Israeli Journal of Aquaculture - Bamidgeh | Year: 2013

Aeromonasis has seriously affected the farming of gibel carp Carassius auratus gibelio, causing a significant and progressive increase in the use of difloxacin. In the present study, a single dose of 20 mg difloxacin per kg body weight was orally administered to healthy and aeromonasis-induced juvenile gibel carp and the pharmacokinetics is described by a two-compartment open model with first-order rate absorption. Compared to the healthy control fish, the absorption, distribution, and elimination rates of difloxacin were greatly reduced and the plasma protein binding level was significantly increased in diseased fish. In addition, as a surrogate marker of antibacterial efficacy, the ratio of maximum plasma concentration (Cmax) to minimum inhibitory concentration (MIC) of difloxacin was calculated; the Cmax/MIC ratios ranged 3.77-18.14 in healthy fish and 1.60-7.70 in diseased fish, indicating that difloxacin at 20 mg/kg body weight cannot ensure successful treatment in aeromonasis-infected fish.

Hu M.,Nanchang University | Hua Q.,Jiangxi Aquaculture Technology Extending Stations | Zhou H.,Jiangxi Fisheries Research Institute | Wu Z.,Guilin University of Technology | Wu X.,Nanchang University
Environmental Biology of Fishes | Year: 2015

The four species of carp present in China (grass carp, black carp, silver carp and bighead carp) are all commercially important freshwater fish species. However, the construction of large-scale hydraulic projects, such as dams, along with other anthropogenic changes to river systems has resulted in the rapid decline in abundance of all four carp species. Indeed, the effect of dams on the reproduction of the four species has become a highly sensitive issue in China. Historically, the Ganjiang River contained 12 spawning grounds for the four species. After the construction of the Wan’an Dam, however, four of these spawning areas were flooded. Three additional dams are currently under construction below the Wan’an Dam. While it is clear that these dams will further alter the hydrological regime in the river, there is currently little information available for the management and protection of carp in this river. In this study, we review published studies to characterize the patterns of larval abundance of the four species of carp in relation to environmental correlates in the Ganjiang River to provide baseline information that can be used to conserve these species. © 2014, The Author(s).

Zhang Y.,Nanjing Agricultural University | Zhang Y.,Jiangxi Fisheries Research Institute | Fu H.,Nanjing Agricultural University | Fu H.,Chinese Academy of Fishery Sciences | And 6 more authors.
Journal of the World Aquaculture Society | Year: 2013

The transformer-2 gene (tra-2) plays a key role in the sexual differentiation regulatory hierarchy. In this study, tra-2 gene homologs designated as Mntra-2 was cloned and characterized from Macrobrachium nipponense. The full-length cDNA of Mntra-2 consists of 1724 bp with an open reading frame (ORF) encoding 192 amino acids, an 827bp 5′-untranslated region (UTR) and a 318bp 3′-UTR. The predicated molecular mass of Mntra-2 was 20.805 kDa with an estimated theoretical isoelectric point of 10.36. The deduced amino acid sequence shares high homology with Penaeus monodon. Real-time quantitative polymerase chain reaction (RT-qPCR) analyses demonstrated that the expression levels of Mntra-2 varied significantly during different developmental stages of embryogenesis, larvae, and post-larvae and in various adult tissues. During embryogenesis, the expression level of Mntra-2 was slightly higher at the cleavage stage than at the blastula stage, and reached the highest level at the nauplius stage. During the larvae, the Mntra-2 expression gradually increased from 1 d larvae post hatch (L1) to L10 and decreased to a lowest level at the end of metamorphosis. During the post-larvae, the Mntra-2 expression was higher level at the 5 d after the metamorphosis (P5). RT-qPCR showed the Mntra-2 mRNA was expressed in ovary, testis, muscle, heart, abdominal ganglion, brain, and intestine with the highest level of expression in muscle and intestine. The results indicate that Mntra-2 is an arthropods tra-2 homolog and probably plays important roles in embryonic development and sex differentiation of M. nipponense. © Copyright by the World Aquaculture Society 2013.

Zhang Y.,Nanjing Agricultural University | Zhang Y.,Jiangxi Fisheries Research Institute | Jiang S.,Chinese Academy of Fishery Sciences | Xiong Y.,Chinese Academy of Fishery Sciences | And 6 more authors.
Turkish Journal of Fisheries and Aquatic Sciences | Year: 2013

The extra sex combs (Esc) gene first identified in Drosophia encodes a transcriptional repressor of homeotic genes belonging to the polycomb group (PcG) gene and is primarily expressed in the early embryos. In this study, we have isolated the full-length cDNA of an Esc gene from the testis of oriental river prawn (Macmbrachium nipponense) according to the established expressed sequence tags (ESTs) information using Rapid Amplification of the cDNA Ends (RACE) technique, designated as MnEsc. The full-length cDNA of MnEsc is 1,461bp in size and has an open reading frame (ORF) of 1,068bp, encoding a 355-amino acid protein. Real-time quantitative PCR analyses revealed that the expression level of MnEsc varied significantly in the developing embryo, postembryonic larval and adult tissues. Real-time quantitative PCR showed the MnEsc gene was expressed in testis, liver, ovary, brain, abdominal ganglion, heart, intestine and muscle with the highest level of expression in ovary and brain. In vivo silencing of the gene, the dsRNA of MnEsc caused a significantly decrease in target gene expression level in brain and ovary tissues, but no exterior appearance change of experimental shrimp was observed. © Published by Central Fisheries Research Institute (CFRI) Trabzon, Turkey in cooperation with Japan International Cooperation Agency (JICA), Japan.

Zhang Y.P.,Nanjing Agricultural University | Zhang Y.P.,Jiangxi Fisheries Research Institute | Qiao H.,Chinese Academy of Fishery Sciences | Zhang W.Y.,Nanjing Agricultural University | And 7 more authors.
Genetics and Molecular Research | Year: 2013

In this study, two Sxl gene homologs, designated as Mnsxl1 and Mnsxl2, were cloned and characterized from the freshwater prawn Macrobrachium nipponense by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnsxl1 and Mnsxl2 showed high sequence homology to the insect Sxl and contained conserved domains in two RNA-binding motifs. Real-time quantitative reverse transcription-polymerase chain reaction (RT-QPCR) showed that the Mnsxl1 and Mnsxl2 genes were expressed in all investigated tissues, with the highest level of expression in the intestine and liver. RT-QPCR also revealed that Mnsxl1 and Mnsxl2 mRNAs expressions were both significantly increased at 5 and 20 days post-larvae after metamorphosis. Thus, the results of the present study imply that Mnsxl1 and Mnsxl2 play complex and important roles in the sex differentiation of M. nipponense. © FUNPEC-RP.

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