Chen Y.,Nanjing Forestry University |
Chen Y.,Jiangxi Academy of science
PloS one | Year: 2014
BACKGROUND: Variegation in flower color is commonly observed in many plant species and also occurs on ornamental peaches (Prunus persica f. versicolor [Sieb.] Voss). Variegated plants are highly valuable in the floricultural market. To gain a global perspective on genes differentially expressed in variegated peach flowers, we performed large-scale transcriptome sequencing of white and red petals separately collected from a variegated peach tree.RESULTS: A total of 1,556,597 high-quality reads were obtained, with an average read length of 445 bp. The ESTs were assembled into 16,530 contigs and 42,050 singletons. The resulting unigenes covered about 60% of total predicted genes in the peach genome. These unigenes were further subjected to functional annotation and biochemical pathway analysis. Digital expression analysis identified a total of 514 genes differentially expressed between red and white flower petals. Since peach flower coloration is determined by the expression and regulation of structural genes relevant to flavonoid biosynthesis, a detailed examination detected four key structural genes, including C4H, CHS, CHI and F3H, expressed at a significantly higher level in red than in white petal. Except for the structural genes, we also detected 11 differentially expressed regulatory genes relating to flavonoid biosynthesis. Using the differentially expressed structural genes as the test objects, we validated the digital expression results by using quantitative real-time PCR, and the differential expression of C4H, CHS and F3H were confirmed.CONCLUSION: In this study, we generated a large EST collection from flower petals of a variegated peach. By digital expression analysis, we identified an informative list of candidate genes associated with variegation in peach flowers, which offered a unique opportunity to uncover the genetic mechanisms underlying flower color variegation.
Zhou H.,Beijing Forestry University |
Zhou H.,Jiangxi Academy of science |
Cheng F.-Y.,Beijing Forestry University |
Wang R.,Beijing Forestry University |
And 2 more authors.
PLoS ONE | Year: 2013
Tree peonies are important ornamental plants worldwide, but growing them can be frustrating due to their short and concentrated flowering period. Certain cultivars exhibit a reblooming trait that provides a valuable alternative for extending the flowering period. However, the genetic control of reblooming in tree peonies is not well understood. In this study, we compared the molecular properties and morphology of reblooming and non-reblooming tree peonies during the floral initiation and developmental processes. Using transcriptome sequencing technology, we generated 59,275 and 63,962 unigenes with a mean size of 698 bp and 699 bp from the two types of tree peonies, respectively, and identified eight differentially expressed genes that are involved in the floral pathways of Arabidopsis thaliana. These differentially regulated genes were verified through a detailed analysis of their expression pattern during the floral process by real time RT-PCR. From this combined analysis, we identified four genes, PsFT, PsVIN3, PsCO and PsGA20OX, which likely play important roles in the regulation of the reblooming process in tree peonies. These data constitute a valuable resource for the discovery of genes involved in flowering time and insights into the molecular mechanism of flowering to further accelerate the breeding of tree peonies and other perennial woody plants. © 2013 Zhou et al.
Liu Y.,Nanchang University |
Shao S.,Nanchang University |
Xu C.,Nanchang University |
Yang X.,Nanchang University |
Lu D.,Jiangxi Academy of science
Materials Letters | Year: 2012
The wear behavior of Mg-1.5Zn-0.15Gd alloy before and after cryogenic treatment (CT) has been investigated by dry sliding wear test and scanning electron microscope (SEM). The experimental result shows that the wear resistance of the alloys has been significantly improved after CT. The observation worn surface indicates that abrasive wear is the main wear mechanism of Mg-1.5Zn-0.15Gd alloy. The friction coefficient and wear rate decrease with the increase of CT duration, which is attributed to the increase of volume fraction of the secondary phase particles and its refinement due to the CT. Moreover, the alloy after cryogenic treatment exhibits a much smoother worn surface. The wear mechanism of the alloy before and after CT has been discussed. © 2012 Elsevier B.V. All rights reserved.
Jiang B.Q.,Nanchang University |
Xiao Z.Q.,Jiangxi Academy of science
Advanced Materials Research | Year: 2011
Electroless plating was used to deposit Ni-P films on quartz optical fiber surface. The optimal bath compositions and operation conditions were determined by orthogonal experiment: nickel sulfate 0.133 mol/L, sodium hypophosphite 0.30 mol/L, propionic acid 0.268 mol/L, boric acid 0.452 mol/L, pH 5.0 and temperature 84°C Stereomicroscope, scanning electron microscope, X-ray diffractometer, energy spectrometer and thermal shock test were used to characterize the morphologies, structures, compositions and adhesion of the Ni-P films. The results showed that a Ni-P film with good smoothness and adhesion as well as low resistivity was fabricated. The kinetic model was established and proven by the experiments. © (2011) Trans Tech Publications, Switzerland.
Hua X.,Nanchang University |
Hua X.,Jiangxi Academy of science |
Li Y.,Nanchang University |
Min Z.,China Electronics Technology Group Corporation
Journal of Alloys and Compounds | Year: 2014
Intermetallic compounds (IMCs) formations of Bi-containing Sn0.7Cu/Cu interface were studied for soldering at four levels of temperatures (240, 260, 280 and 300 °C) and isothermal aging at 150 °C. It was found that addition of Bi into the Sn0.7Cu eutectic solder caused the excessive formations of IMCs during the soldering reaction and thereafter in aging condition. The interfacial IMC layer was composed of Cu60- Sn5 and Cu3Sn layers after liquid soldering for Sn0.7Cu/Cu, Sn0.7Cu0.7Bi/Cu and Sn0.7Cu1.3Bi/Cu joints. With the increasing soldering temperature, the thickness of IMC layers (including Cu6Sn5 and Cu3Sn IMCs) increased linearly. After various days of aging, a comparatively planar IMC layer at the solder/Cu interface was observed than that of the as-soldered joints. The growth of IMC layer during aging for Sn0.7Cu, Sn0.7Cu0.7Bi and Sn0.7Cu1.3Bi solders followed the diffusion control mechanism. And the interfacial IMC layers were thicker with higher Bi content in solder alloy since the Bi could result in more chemical bonds between Cu atoms or between Cu and Sn atoms to be broken, which made more Cu and Sn atoms activated. © 2013 Elsevier B.V. All rights reserved.
Jiang B.Q.,Nanchang University |
Xiao Z.Q.,Jiangxi Academy of science
Advanced Materials Research | Year: 2012
The waste wood dust was utilized to prepare the copper oxide loaded activated carbon for the treatment of the printing and dyeing wastewater (acid red GR wastewater). The response surface assisted with Design-Expert 7.0 software was used to optimize the process. The secondary multiple regression models for the color and COD removal rates were established and proven to be significant. The optimum process conditions determined by the software were: copper nitrate (0.5mol/L) 15mL, ratio of liquid to solid 56, activation temperature 690 °C and activation time 2.1h, under which the color and COD removal rates reached 99.8% and 88.34%, respectively. © (2012) Trans Tech Publications, Switzerland.
Qiu H.,Nanchang University |
Yan H.,Nanchang University |
Hu Z.,Nanchang University |
Hu Z.,Jiangxi Academy of science
Journal of Alloys and Compounds | Year: 2013
The effects of samarium (Sm) additions (0-0.9 wt.%) on the microstructures and mechanical properties of Al-7Si-0.7Mg alloys have been studied in this article. The microstructures of the as-cast samples were examined by optical microscopy (OM) and scanning electron microscopy (SEM). The experimental results indicated that the rare earth Sm affected the secondary dendrite arm spacing (SDAS) of Al-7Si-0.7Mg alloy. And it was found that Sm had great modification effects on the microstructures of eutectic silicon. When 0.6 wt.% Sm was added to the alloy, the coarse plate-like eutectic silicon was fully modified into a fine fibrous structure; the dendrites of Al-7Si-0.7Mg alloy was best refined. The mechanical properties were investigated by tensile test. The findings indicate that the tensile properties and elongation were improved by the addition of Sm. And a good combination of ultimate tensile strength (215 MPa) and elongation (3.3%) was obtained when the Sm addition was up to 0.6 wt.%. Furthermore the results of thermal analysis reveal that Sm addition had marked effects on eutectic temperature and the latent heat ΔHR on remelting behavior. © 2013 Elsevier B.V. All rights reserved.
Guo S.F.,Southwest University |
Zhang H.J.,Chongqing University |
Liu Z.,Hong Kong Polytechnic University |
Chen W.,Hong Kong Polytechnic University |
Xie S.F.,Jiangxi Academy of science
Electrochemistry Communications | Year: 2012
It is well recognized that amorphous alloys usually offer much higher resistance to corrosion over their conventional counterparts in most aqueous solutions. Whereas, in this work, we reported an anomalous corrosion resistance of an arc melting Zr 62.3Cu 22.5Fe 4.9Al 6.8Ag 3.5 crystalline alloy, which was superior to that of the same composition in glassy state in simulated seawater. It was revealed that the easy formation of a highly protective Zr- and Al-enriched oxide layer at the surface of the crystalline alloy is responsible for the extremely high corrosion resistance. The current finding not only presents an excellent alloy candidate for potential application in seawater but provides a new insight into the understanding of corrosion behavior of amorphous/crystalline alloys in demanding corrosive media. © 2012 Elsevier B.V.
Wang H.,Huazhong Agricultural University |
Jin L.,Jiangxi Academy of science |
Zhang H.,Huazhong Agricultural University
Journal of Applied Microbiology | Year: 2011
Aims: To (i) identify the bacterial communities in the gut of oriental fruit fly (Bactrocera dorsalis) adult and (ii) determine whether the different surroundings and diets influence the bacteria composition. Methods and Results: Polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) fingerprinting was used to investigate bacterial diversity in the oriental fruit fly adult gut. The 16S rDNA cloned libraries from the intestinal tract of laboratory-reared (LR), laboratory sterile sugar-reared (LSSR) and field-collected (FC) populations of oriental fruit fly were compared. Phylogenetic analysis of 16S rDNA revealed that Gammaproteobacteria were dominant in the all samples (73·0-98·3%). Actinobacteria and Firmicutes were judged to be major components of a given library as they constituted 10% or more of the total clones of such library. The Flavobacteria, Deltaproteobacteria, Bacteroidetes and Alphaproteobacteria were observed in small proportions in various libraries. Further phylogenetic analyses indicated common bacterial phylotypes for all three libraries, e.g. those related to Klebsiella, Citrobacter, Enterobacter, Pectobacterium and Serratia. libshuff analysis showed that the bacterial communities of B. dorsalis from the three populations were significantly different from each other (P<0·0085). Conclusions: (i) The intestinal tract of B. dorsalis adult contains a diverse bacterial community, some of which are stable. (ii) Different environmental conditions and food supply could influence the diversity of the harboured bacterial communities and increase community variations. Significance and Impact of the Study: Comparison of the microbial compositions and common bacterial species found in this paper may be very important for the biocontrol of B. dorsalis. © 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology.
Ling W.X.,Northwest University, China |
Ling W.X.,Jiangxi Academy of science |
Zhong Z.,Northwest University, China
Turkish Journal of Agriculture and Forestry | Year: 2012
This study was carried out to elucidate the relationship between seasonal variation of rooting ability and inner conditions of the cuttings of Tetraploid Locust, and to determine the optimum time for rooting. It was conducted in a glasshouse at the Experimental Nursery of the College of Forestry, North-West Agriculture and Forestry University, Yang Ling, China, during 2009. Tetraploid Locust cuttings were taken from healthy trees of 3-year-old field-grown mother-stock orchard. Cuttings (15 cm in length and 10-12 mm in diameter) were collected and planted on 15 February, 15 March, 15 May, 15 June, 15 July, and 15 August. The results obtained 2 months aft er each planting date showed that planting the cuttings on 15 May produced significant increases in rooting percentage compared to the other planting dates. The highest total carbohydrates concentration and carbohydrate/nitrogen (C/N) ratio were recorded in the basal parts of the stem cuttings planted on 15 May, either before planting or 35 days aft er planting. A ositive relationship of rooting percentage was found between total carbohydrate concentration and C/N ratio. No consistent relationship was established between total nitrogen and rooting percentage. Results also showed a high and a low negative relationship of the rooting percentage of the cuttings between indole-3-acetic acid (IAA) concentration and gibberellins (GAs) concentration, respectively. In addition, 35 days aft er planting, a positive relationship was detected between abscisic acid (ABA) concentration and the rooting percentage. From the results obtained, 15 May may be recommended as the ideal planting date for improving the rooting and the vegetative growth of Tetraploid Locust stem cuttings compared to the other investigated planting dates. © TÜBİTAK.