Yang C.,Nanjing Forestry University |
Yang C.,Jiangxi Academy of Forestry |
Xu M.,Nanjing Forestry University |
Xuan L.,Nanjing Forestry University |
And 2 more authors.
Gene | Year: 2014
The auxin response factor (ARF) family of transcription factors is a crucial component of auxin signaling and plays important roles regulating numerous growth and developmental processes in plants. We isolated and characterized 20 ARF genes involved in adventitious root development of Populus. Multiple protein sequence alignments revealed that the PeARF proteins contained a highly conserved region in their N-terminal portion corresponding to the DNA-binding domain of the Arabidopsis ARF family. Except for PeARF3.1, PeARF3.2, PeARF17.1 and PeARF17.2, the PeARF proteins contained a carboxyl-terminal domain related to the Arabidopsis domains III and IV, which are involved in homo- and heterodimerization. The exon-intron structures of the PeARF genes were determined by aligning cDNA and genomic sequences. As expected, most PeARF genes had a similar distribution of exon-intron structures. Temporal expression patterns of these genes were profiled during adventitious root development. All 20 PeARF genes were expressed in root, stem and leaf in a dynamic manner. Transient expression assays with Populus protoplasts demonstrated that these PeARFs were localized to the nucleus. These results suggest that PeARFs may play diverse regulatory roles in adventitious root development of Populus and contribute to improving our understanding of conserved and divergent aspects of auxin signaling in various species. © 2014 Elsevier B.V.
Liu P.,Jiangxi Academy of Forestry
Dong wu xue yan jiu = Zoological research / "Dong wu xue yan jiu" bian ji wei yuan hui bian ji | Year: 2012
Habitat selection of four sympatric pheasants (Syrmaticus ellioti, Lophura nycthemera, Pucrasia macrolopha and Bumbusicola thoracica) was studied in Guanshan National Nature Reserve, Jiangxi, China from October 2009 to November 2010. We measured seventeen ecological factors across 388 sites used by the four pheasants by direct observation in six transects. The results show that the pheasants preferred broad-leaved forest and coniferous forest and a sunny or semi-shaded slope. There were differences in elevation, degree of slope, arbor coverage, shrub coverage, shrub quantity, shrub height, herbage cover, herbage species, herbage quantity, leaf litter coverage, distance to water and arbor species between the habitat selected by the four pheasants. Canonical scores indicated that the four pheasants differed in habitat selection to some extent. A stepwise discriminant analysis revealed that elevation, herbage quantity, shrub height, distance to water, shrub cover, arbor cover, degree of slope, leaf litter cover, shrub quantity and herbage cover were the critical factors distinguishing habitat selection between the species. Predicted classification accuracy of the model was 74.7%.
Huang Z.H.,Jinggangshan University |
Tu F.Y.,Jiangxi Academy of Forestry
Genetics and Molecular Research | Year: 2016
The control region is considered to be one of the most variable parts of animal mitochondrial DNA (mtDNA). We compared the mtDNA control region from 37 species representing 14 genera and 4 subfamilies of Ranidae, to analyze the evolution of the control region and to determine their phylogenetic relationship. All the Ranidae species had a single control region, except four species that had two repeat regions. The control region spanned the region between the Cyt b and tRNAleu genes in most of the Ranidae species. The length of the control region sequences ranged from 1186 bp (Limnonectes bannaensis) to 6746 bp (Rana kunyuensis). The average genetic distances among the species varied from 1.94% (between R. chosenica and R. plancyi) to 113.25% (between Amolops ricketti and Euphlyctis hexadactylus). The alignment of three conserved sequence blocks was identified. However, conserved sequence boxes F to A were not found in Ranidae. A maximum likelihood method was used to reconstruct the phylogenetic relationship based on a general time reversible + gamma distribution model. The amount of A+T was higher than G+C across the whole control region. The phylogenetic tree grouped members of the respective subfamilies into separate clades, with the exception of Raninae. Our analysis supported that some genera, including Rana and Amolops, may be polyphyletic. Control region sequence is an effective molecular mark for Ranidae phylogenetic inference. © 2016 The Authors.
Huang Z.H.,Jinggangshan University |
Tu F.Y.,Jiangxi Academy of Forestry
Genetics and Molecular Research | Year: 2016
In this study, we determined the complete nucleotide sequence of the mitochondrial (mt) DNA of the paddy frog Fejervarya multistriata. mtDNA is 17,750-bp long and contains 13 protein-coding regions, 2 ribosomal RNA, non-coding genes, and 23 tRNA because of the presence of an extra copy of tRNA-Met. The gene arrangements among two related species of Fejervarya were compared, and the combined mtDNA data were subjected to a phylogenetic analysis. Interestingly, we observed a unique translocation of the tRNA-Leu gene, similar to that reported in previous studies on two Fejervarya species. Phylogenetic analyses supported the classification into two evolutionary clades, Ranidae and Dicroglossidae, as well as placement of Hylarana guentheri in the genus Babina. Our results suggested that Fejervarya limnocharis and Fejervarya multistriata may be conspecific,because of its low pairwise genetic distance. However, these results must be further validated with additional analyses. © 2016 The Authors.
Wang Q.,CAS Shenyang Institute of Applied Ecology |
Xiao F.,Jiangxi Academy of Forestry |
He T.,CAS Shenyang Institute of Applied Ecology |
He T.,University of Chinese Academy of Sciences |
Wang S.,CAS Shenyang Institute of Applied Ecology
Annals of Forest Science | Year: 2013
Aims: Globally, extensive areas of native forest have been almost replaced by plantations to meet the demands for timber, fuel material and other forest products. This study aimed to evaluate the effects of forest conversion on labile soil organic C (SOC), soil respiration, and enzyme activity, and to quantify their relationship in subtropical forest ecosystems. Methods: Surface mineral soil (0-20 cm) was collected from a Cunninghamia lanceolata Hook. plantation, Pinus massoniana Lamb. plantation, Michelia macclurei Dandy plantation, and an undisturbed native broadleaf forest. Soil microbial biomass C, dissolved organic C, permanganate-oxidizable C, basal respiration, and six enzyme activities were investigated. Results: Soil microbial biomass C was higher by 45.9 % in native broadleaf forest than that in M. macclurei Dandy plantation. The ratio of soil microbial biomass C to total SOC was 27.6 % higher in the M. macclurei Dandy plantation than in the native broadleaf forest. The soil respiration increased by 25.2 % and 21.7 % after conversion from native broadleaf forest to P. massoniana Lamb. and M. macclurei Dandy plantations respectively. The effects of forest conversion on the soil enzyme activities differed among the tree species. Soil microbial biomass C had higher correlation with soil respiration than with the other SOC fractions. Moreover, soil microbial biomass C was positively correlated with urease and negatively correlated with cellulase activity. Soil respiration had higher correlation with soil microbial biomass C, dissolved organic C and permanganate-oxidizable C. Conclusion: Forest conversion affected the soil microbial biomass C, soil respiration, invertase, cellulase, urease, catalase, acid phosphatase, and polyphenol oxidase activities, but their response depended on tree species. Soil respiration was mainly controlled by labile SOC, not by total SOC. © 2013 INRA and Springer-Verlag France.
Shao Q.,Zhejiang Agriculture And forestry University |
Deng Y.,Jiangsu Academy of Agricultural Sciences |
shen H.,Changzhou Health College |
Fang H.,Jiangxi Academy of Forestry |
Zhao X.,Zhejiang Agriculture And forestry University
International Journal of Biological Macromolecules | Year: 2011
A 15-run Box-Behnken design (BBD) was used to optimize the extraction conditions of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg. Three factors such as extraction temperature (°C), extraction time (h), and ratio of water to raw material were investigated. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also examined using the appropriate statistical methods. The adjusted coefficient of determination (RAdj2) for the model was 0.9754, and the probability value (P= 0.001) demonstrated a high significance for the regression model. The optimum extraction conditions were found to be: optimized extraction temperature 83.3 °C, extraction time 1.55. h and ratio of water to raw material 29.48. Under these conditions, the mean extraction yield of polysaccharides was 5.182 ± 0.093%, which was in good agreement with the predicted model value. © 2011 Elsevier B.V.
Huang Z.,Jinggangshan University |
Tu F.,Jiangxi Academy of Forestry |
Murphy R.W.,Royal Ontario Museum
Biochemical Systematics and Ecology | Year: 2016
The Oriental turtle dove, Streptopelia orientalis (Columbiformes: Columbidae) is an extremely widespread species. We investigate and analyze its complete mitochondrial genome. The complete mtDNA genome, a circular DNA molecule of 17,130bp, contains of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a control region. Its base composition is A 29.7%, T 23.9%, G 14.1%, C 32.3%. Similar to other birds and turtles, the Oriental turtle dove has an extra base "C" at position 174 of gene Nd3. The most frequently codon usage is CUA (7.11%), followed by AUC (5.52%), CUC (4.97%) and UUC (4.36%). Genetic distance analyses indicate that the difference between Macropygia phasianella vs Spilopelia senegalensis is lower than that Spilopelia chinensis vs S. orientalis based on both the standard barcoding gene Cox1 and Cytb. Genetic distances coincide with morphological, behavioral and phylogenetic analyses that suggest the assignment of chinensis and senegalensis to the genus Spilopelia, following the latest taxonomic system of the IUCN. © 2016 Elsevier Ltd.
PubMed | Anhui Science and Technology University, Jiangxi Academy of Forestry, Nanjing University and Jinggangshan University
Type: Journal Article | Journal: Indian journal of microbiology | Year: 2016
Acidic red soil from a forest in Jiangxi Province was selected to isolate aluminum (Al)-resistant microbes, from which eight fungi were isolated. Two strains (S4 and S7) were found to be extremely tolerant to Al concentrations of up to 550mmolL(-1) and could grow at low pH levels (3.20-3.11). Morphological and 26S rDNA sequence analyses indicated that strain S4 belonged to Eupenicillium, while strain S7 was an unclassified Trichocomaceae. Further investigation showed that both strains were endowed with the ability to resist Al; strain S4 accumulated such a substantial amount of Al that its growth was limited to a larger extent than strain S7. The lower amounts of Al adsorbed in the mycelium and the much larger amounts of Al retained in the medium, in addition to the color change of the culture solution, implied that these two strains may resist Al by preventing Al from entering the cell and by chelating Al by secreting unique metabolites outside of the cell.
PubMed | Pingdingshan University and Jiangxi Academy of Forestry
Type: Journal Article | Journal: Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis | Year: 2016
The Gansu mole Scapanulus oweni belongs to family Talpidae, and is distributed in the Central and Southwest China. In this study, the total mitochondrial genome of S. oweni was firstly determined. The genome is 16,826 bases in length and contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a displacement loop region, with a base composition of A 34.0%, G 13.5%, T 28.7% and C 23.9%. All of the protein-coding genes initiate with the orthodox ATG start codon execpt for Nd2 and Nd3 begin with ATT, Nd4 and Nd5, start with GTG and ATA, respectively. Four types of stop codons are used by the coding genes, including TAA for Nd1, Cox1, Cox2, Atp8, Atp6, Nd4L, Nd5, Nd6, TAG for Nd2, AGA for Cytb, and an incomplete stop codon T for Cox3, Nd3 and Nd4. The mito-genomic data of Gansu mole, S. oweni will be useful in determining its taxonomic status within Talpidae.
Xiong Z.-Y.,Jiangxi Academy of Forestry |
Xiao F.-M.,Jiangxi Academy of Forestry |
Xu X.,Centers for Disease Control and Prevention |
Wu Y.-F.,Jiangxi Academy of Forestry |
Jiang X.-M.,Jiangxi Academy of Forestry
Zhongguo Zhongyao Zazhi | Year: 2013
Borneol is a major component of many medicinal plant essential oils, as well as a popular traditional Chinese medicine. This essay collects the results of the latest domestic and foreign studies, and summarizes and analyzes its activity and reaction mechanism on analgesia, putridity elimination and flesh regeneration, and repair of damaged cells. Moreover, it proposes problems concerning borneol during medical studies, providing support for the in-depth study and exploration of efficacies of precious traditional Chinese medicines as well as the effective utilization and development of innovative medicines.