Jiangxi Academy of Agricultural science

Nanchang, China

Jiangxi Academy of Agricultural science

Nanchang, China
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Dong A.,Jiangxi Agricultural University | Xie J.,Jiangxi Academy of Agricultural science | Wang W.,Jiangxi Agricultural University | Yu L.,Jiangxi Agricultural University | And 2 more authors.
Journal of Hazardous Materials | Year: 2010

A novel method was proposed to prepare amino starch by reacting ethylenediamine with previously synthesized dialdehyde starch. Different factors affecting the preparation, i.e., ethylenediamine concentration, ethylenediamine:dialdehyde starch molar ratio, pH, duration and temperature have been studied. The modified starch was characterized by IR spectroscopy, elemental analysis, X-ray diffraction and differential scanning calorimetry. The amino starch derivatives were evaluated as adsorbents. The adsorption activity for heavy metals such as Cu(II) and Cr(VI) by crosslinked amino starch was studied in terms of adsorption amount, kinetics and isotherm; and adsorbent reuse were also studied. Adsorption processes for Cu(II) and Cr(VI) on crosslinked amino starch fit a Langmuir isotherm, and adsorption for Cu(II) and Cr(VI) from aqueous solution was endothermic reactions. Crosslinked amino starch was very effective for the adsorption of Cu(II) and Cr(VI), and efficient in capacity, recycled. © 2010 Elsevier B.V.

Chen H.-P.,Nanchang University | Zhou W.,Nanjing Medical University | Kang L.-M.,Nanchang University | Yan H.,Jiangxi Academy of Agricultural science | And 3 more authors.
Neurochemical Research | Year: 2014

MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression post-transcriptionally by binding to their cognate target mRNAs. Emerging evidence suggests that miRNAs are critical regulators of neuronal functions. The expression pattern of miRNAs in the peripheral nervous system after peripheral nerve injury suggest that miRNAs may have important and yet unknown roles in the mechanisms of pain. Thus, we examined the role of miR-96 in neuropathic pain using a rat model of the condition chronic constriction sciatic nerve injury (CCI). We found that miR-96 alleviated neuropathic pain. The level of miR-96 was decreased within the ipsilateral dorsal root ganglion (DRG) after peripheral nerve injury but the Nav1.3 level was increased. Specifically, Intrathecal administration of miR-96 suppressed the expression of Nav1.3 induced by CCI. Further examination revealed that miR-96 inhibited the Nav1.3 mRNA expression in the embryonic DRG neurons in vitro. Our findings suggest that miR-96 participate in the regulation of neuropathic pain through inhibiting the expression of Nav1.3 in the DRG of CCI rats. © 2013 Springer Science+Business Media New York.

Zhang D.,CAS Wuhan Institute of Hydrobiology | Zhang D.,Jiangxi Academy of Agricultural science | Deng X.,CAS Wuhan Institute of Hydrobiology | Xie P.,CAS Wuhan Institute of Hydrobiology | And 2 more authors.
Food Chemistry | Year: 2013

Bioaccumulation and risk assessment of microcystins (MCs) in muscle of silver carp (Hypophthalmichthys molitrix) from eight eutrophic lakes along the Yangtze River of China were examined by using liquid chromatography electrospray ionisation mass spectrometry. MCs contents in seston collected from these eutrophic lakes ranged from 0.02 to 21.7 μg/L. MCs concentrations in silver carp muscle samples varied from 0.014 to 0.036 μg/g DW with an average of 0.028 μg/g DW. The total length of silver carp showed a significant negative correlation with MCs concentrations in their muscle (r = -0.85, p < 0.05), suggesting that MCs accumulation in silver carp muscle seems to be size dependent. EDI values of MCs in fish muscle from these eight eutrophic lakes varied from 0.0027 to 0.0071 μg/kg day, which was much lower than the TDI value of 0.04 μg/kg day previously established by WHO, indicating that it is safe to consume silver carp muscle from eutrophic lakes in China. © 2013 Elsevier Ltd. All rights reserved.

Liao Q.G.,Jiangxi Academy of Agricultural science | Zhou Y.M.,Jiangxi Academy of Agricultural science | Luo L.G.,Jiangxi Academy of Agricultural science | Wang L.B.,Fuzhou Tobacco Company | Feng X.H.,Fuzhou Tobacco Company
Microchimica Acta | Year: 2014

We report on a method for the determination of twelve herbicides using solid-liquid-solid dispersive extraction (SLSDE), followed by dispersive liquid-liquid micro-extraction (DLLME) and quantitation by gas chromatography with triple quadrupole mass spectrometric detection. SLSDE was applied to the extraction of herbicides from tobacco samples using multi-walled carbon nanotubes (MWCNTs) as clean-up adsorbents. The effect of the quantity of MWCNTs on SLSDE, and of type and volume of extraction and disperser solvents and of salt effect on DLLME were optimized. Good linearity is obtained in the 5.0 - 500 μg kg-1 concentration range, with regression coefficients of >0.99. Intra-day and inter-day repeatability, expressed as relative standard deviations, are between 3 and 9 %. The recoveries in case of herbicide-spiked tobacco at concentration levels of 20.0, 50.0 and 100.0 g kg-1 ranged from 79 to 105 %, and LODs are between 1.5 and 6.1 μg kg-1. All the tobacco samples were found to contain butralin and pendimethalin at levels ranging from 15.8 to 500.0 μg kg-1. [Figure not available: see fulltext.] © 2013 Springer-Verlag Wien.

Wei Y.H.,Jiangxi Academy of Agricultural science | Zhang J.Y.,Jiangxi Academy of Agricultural science | Zhang D.W.,Jiangxi Academy of Agricultural science | Luo L.G.,Jiangxi Academy of Agricultural science | Tu T.H.,Jiangxi Academy of Agricultural science
Food Chemistry | Year: 2014

A quick and accurate method was devised to determine Se, As, Ba, Ca, Cd, Cr, Cu, Fe, Mg, Mn, Ni, Pb, Sr and Zn in Se-rich rice samples by microwave digestion and inductively coupled plasma-mass spectrometry (ICP-MS). Spectral interferences on Se were eliminated using methane as a reaction gas in the dynamic reaction cell (DRC). Rhodium was used as an internal standard to compensate for sample matrix effects. A rice-certified reference material (CRM) (GBW 10010) was used to verify the accuracy of the method. The method detection limits were 0.001-0.03 mg/kg, analyte recoveries were 85-108% and precisions (RSDs) ranged from 2.1% to 5.8%. Correlation analysis showed that the Se concentrations in the Se-rich rice samples correlated well with the Cu concentrations (r = 0.53, p < 0.05). © 2014 Elsevier Ltd. All rights reserved.

Yao J.,Jiangxi Academy of Agricultural science | Guo G.S.,Sun Yat Sen University | Ren G.H.,Sun Yat Sen University | Liu Y.H.,Sun Yat Sen University
Journal of Molecular Catalysis B: Enzymatic | Year: 2014

Tannases, tannin acylhydrolases, are an important group of biotechnologically relevant enzymes which were utilized in a number of industrial applications, including the manufacture of instant tea, beer, fruit juices, some wines and gallic acid production. Tannases are by and large produced by microorganisms including Aspergillus, Paecilomyces, Lactobacillus and Bacillus. Tannases are generally produced on tannic carbon such as tannic acid, wheat bran, tea and coffee husk extract. Microbial tannases are mostly induced extracellular enzyme and produced by submerged fermentation and solid-state fermentation. The enzyme is most commonly purified by hydrophobic interaction chromatography in addition to reverse micelle. Most tannases can act in a wide range of temperature and pH, although tannases with acidic pH optima are more common. A sequence-based classification spreads tannases in many families thus reflecting the variety of molecules. Furthermore, tannase from Lactobacillus plantarum had been characterized by three-dimensional architecture. In recent years, a novel approach, metagenomic, was developed to exploring novel tannase from natural communities. © 2013 Elsevier B.V.

Zhang J.,University of Chinese Academy of Sciences | Liu J.,Jiangxi Academy of Agricultural science | Liu R.,University of Chinese Academy of Sciences
Bioresource Technology | Year: 2015

In this study, the effects of pyrolysis temperature and heating time on the yield and physicochemical and morphological properties of biochar obtained from straw and lignosulfonate were investigated. As pyrolysis temperature increased, pH, ash content, carbon stability, and total content of carbon increased while biochar yield, volatile matter, total content of hydrogen, oxygen, nitrogen and sulfur decreased. The data from scanning electron microscope image and nuclear magnetic resonance spectra indicated an increase in porosity and aromaticity of biochar produced at a high temperature. The results showed that feedstock types could also influence characteristics of the biochar with absence of significant effect on properties of biochar for heating time. © 2014 Elsevier Ltd.

Chen X.L.,Shanghai Key Laboratory of Veterinary Biotechnology | Chen X.L.,Jiangxi Academy of Agricultural science | Gong L.Z.,Shanghai Key Laboratory of Veterinary Biotechnology | Xu J.X.,Shanghai Key Laboratory of Veterinary Biotechnology
Animal | Year: 2013

In this study, antioxidant capability and protective effect of probiotics on reproductive damage induced by diet oxidative stress were investigated. Thirty male Sprague-Dawley rats were randomly divided into three groups with 10 rats in each group. The control group consumed a normal standard diet (5% fat, w/w). The other two treatment groups were fed with a high-fat diet (20% fat, w/w), and a high-fat diet supplemented with 2% probiotics (w/w), respectively. At the end of the experimental period, that is, after 6 weeks, rats were killed. Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), contents of nitric oxide (NO) free radical and malondialdehyde (MDA) in serum and sperm suspension were examined. Sperm parameters including sperm concentration, viability, motility and DNA integrity were analyzed. The results showed that high-fat diet could induce oxidative stress, shown as significant increases in lipid peroxidation, NO free radical, significant decrease in activities of SOD, GSH-Px, significant reduction in sperm concentration, viability and motility, and damage in sperm DNA (P < 0.05), compared with the control group. These alterations were significantly reversed in the probiotics-supplemented group and had no significant difference in antioxidant capability, lipid peroxidation and sperm parameters compared with the control group. The percentage of sperm with DNA damage was significantly lower than the high-fat diet group and still higher than the control group, which means that probiotics could attenuate sperm damage to some extent. The present results indicated that dietary probiotics had antioxidant activity and the protective effect against sperm damage induced by high-fat diet to some extent. © The Animal Consortium 2012.

Liao Q.G.,Jiangxi Academy of Agricultural science | Li W.H.,Jiangxi Academy of Agricultural science | Luo L.G.,Jiangxi Academy of Agricultural science
Analytical and Bioanalytical Chemistry | Year: 2013

A method based on ultrasound-assisted emulsification-microextraction (USAEME) was proposed in this contribution for the determination of ethyl carbamate (EC) in alcoholic beverages using gas chromatography coupled to triple quadrupole mass spectrometry. To achieve the determination of EC in alcoholic beverages, the influences on the extraction efficiency of type and volume of extraction solvent, temperature, ionic strength, alcohol content, and extraction time were studied, once the extraction solvent had been selected. The optimized conditions were 200.0 μL of chloroform at 30 C during 5 min with 15 % (m/v) sodium chloride addition. The detection limit, relative standard deviations, linear range, and recoveries under the optimized conditions were 0.03 μg L-1, 4.2-6.1 %, 0.1-50.0 μg L-1, and 80.5-87.9 %, respectively. Moreover, the feasibility of the present method was also validated by real samples. To the best of our knowledge, this is the first time that USAEME has been applied to determine a strongly hydrophilic compound in alcoholic beverages. [Figure not available: see fulltext.] © 2013 Springer-Verlag Berlin Heidelberg.

Wei Y.,Jiangxi Academy of Agricultural science | Zhang J.,Jiangxi Academy of Agricultural science | Zhang D.,Jiangxi Academy of Agricultural science | Tu T.,Jiangxi Academy of Agricultural science | Luo L.,Jiangxi Academy of Agricultural science
Ecotoxicology and Environmental Safety | Year: 2014

Concentrations of As, Cd, Cr, Cu, Hg, Ni, Pb, and Zn in the muscle of eleven fish species (bighead carp, bream, catfish, carp, crucian, Culter alburnus, grass carp, mandarin fish, white semiknife carp, silver carp, and yellow catfish) from Poyang Lake were analysed using inductive coupling plasma mass spectrometry. Metal levels in other organs (e.g., bladder, gill, kidney, liver, and spleen) of bighead carp, carp, grass carp, and silver carp were also determined. The results showed that metal concentrations in the muscle of all fish species were significantly lower than the proposed limits. Heavy metal concentrations were found to be substantially higher in benthic fish than in pelagic fish. Higher Hg contents were observed in predatory fish. In addition, various metals showed different affinity to fish organs. Hg was the most abundant in muscle, while Ni and Pb concentrations were highest in gills, Cd and Zn concentrations were highest in kidneys, and Cu was most commonly found in livers. Estimations of health risks revealed no evidence of potential threats to consumers. © 2014 Elsevier Inc.

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