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Zhang D.,CAS Wuhan Institute of Hydrobiology | Zhang D.,Jiangxi Academy of Agricultural science | Deng X.,CAS Wuhan Institute of Hydrobiology | Xie P.,CAS Wuhan Institute of Hydrobiology | And 2 more authors.
Food Chemistry | Year: 2013

Bioaccumulation and risk assessment of microcystins (MCs) in muscle of silver carp (Hypophthalmichthys molitrix) from eight eutrophic lakes along the Yangtze River of China were examined by using liquid chromatography electrospray ionisation mass spectrometry. MCs contents in seston collected from these eutrophic lakes ranged from 0.02 to 21.7 μg/L. MCs concentrations in silver carp muscle samples varied from 0.014 to 0.036 μg/g DW with an average of 0.028 μg/g DW. The total length of silver carp showed a significant negative correlation with MCs concentrations in their muscle (r = -0.85, p < 0.05), suggesting that MCs accumulation in silver carp muscle seems to be size dependent. EDI values of MCs in fish muscle from these eight eutrophic lakes varied from 0.0027 to 0.0071 μg/kg day, which was much lower than the TDI value of 0.04 μg/kg day previously established by WHO, indicating that it is safe to consume silver carp muscle from eutrophic lakes in China. © 2013 Elsevier Ltd. All rights reserved.

Chen H.-P.,Nanchang University | Zhou W.,Nanjing Medical University | Kang L.-M.,Nanchang University | Yan H.,Jiangxi Academy of Agricultural science | And 3 more authors.
Neurochemical Research | Year: 2014

MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression post-transcriptionally by binding to their cognate target mRNAs. Emerging evidence suggests that miRNAs are critical regulators of neuronal functions. The expression pattern of miRNAs in the peripheral nervous system after peripheral nerve injury suggest that miRNAs may have important and yet unknown roles in the mechanisms of pain. Thus, we examined the role of miR-96 in neuropathic pain using a rat model of the condition chronic constriction sciatic nerve injury (CCI). We found that miR-96 alleviated neuropathic pain. The level of miR-96 was decreased within the ipsilateral dorsal root ganglion (DRG) after peripheral nerve injury but the Nav1.3 level was increased. Specifically, Intrathecal administration of miR-96 suppressed the expression of Nav1.3 induced by CCI. Further examination revealed that miR-96 inhibited the Nav1.3 mRNA expression in the embryonic DRG neurons in vitro. Our findings suggest that miR-96 participate in the regulation of neuropathic pain through inhibiting the expression of Nav1.3 in the DRG of CCI rats. © 2013 Springer Science+Business Media New York.

Yao J.,Jiangxi Academy of Agricultural science | Guo G.S.,Sun Yat Sen University | Ren G.H.,Sun Yat Sen University | Liu Y.H.,Sun Yat Sen University
Journal of Molecular Catalysis B: Enzymatic | Year: 2014

Tannases, tannin acylhydrolases, are an important group of biotechnologically relevant enzymes which were utilized in a number of industrial applications, including the manufacture of instant tea, beer, fruit juices, some wines and gallic acid production. Tannases are by and large produced by microorganisms including Aspergillus, Paecilomyces, Lactobacillus and Bacillus. Tannases are generally produced on tannic carbon such as tannic acid, wheat bran, tea and coffee husk extract. Microbial tannases are mostly induced extracellular enzyme and produced by submerged fermentation and solid-state fermentation. The enzyme is most commonly purified by hydrophobic interaction chromatography in addition to reverse micelle. Most tannases can act in a wide range of temperature and pH, although tannases with acidic pH optima are more common. A sequence-based classification spreads tannases in many families thus reflecting the variety of molecules. Furthermore, tannase from Lactobacillus plantarum had been characterized by three-dimensional architecture. In recent years, a novel approach, metagenomic, was developed to exploring novel tannase from natural communities. © 2013 Elsevier B.V.

Chen X.L.,Shanghai Key Laboratory of Veterinary Biotechnology | Chen X.L.,Jiangxi Academy of Agricultural science | Gong L.Z.,Shanghai Key Laboratory of Veterinary Biotechnology | Xu J.X.,Shanghai Key Laboratory of Veterinary Biotechnology
Animal | Year: 2013

In this study, antioxidant capability and protective effect of probiotics on reproductive damage induced by diet oxidative stress were investigated. Thirty male Sprague-Dawley rats were randomly divided into three groups with 10 rats in each group. The control group consumed a normal standard diet (5% fat, w/w). The other two treatment groups were fed with a high-fat diet (20% fat, w/w), and a high-fat diet supplemented with 2% probiotics (w/w), respectively. At the end of the experimental period, that is, after 6 weeks, rats were killed. Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), contents of nitric oxide (NO) free radical and malondialdehyde (MDA) in serum and sperm suspension were examined. Sperm parameters including sperm concentration, viability, motility and DNA integrity were analyzed. The results showed that high-fat diet could induce oxidative stress, shown as significant increases in lipid peroxidation, NO free radical, significant decrease in activities of SOD, GSH-Px, significant reduction in sperm concentration, viability and motility, and damage in sperm DNA (P < 0.05), compared with the control group. These alterations were significantly reversed in the probiotics-supplemented group and had no significant difference in antioxidant capability, lipid peroxidation and sperm parameters compared with the control group. The percentage of sperm with DNA damage was significantly lower than the high-fat diet group and still higher than the control group, which means that probiotics could attenuate sperm damage to some extent. The present results indicated that dietary probiotics had antioxidant activity and the protective effect against sperm damage induced by high-fat diet to some extent. © The Animal Consortium 2012.

Dong A.,Jiangxi Agricultural University | Xie J.,Jiangxi Academy of Agricultural science | Wang W.,Jiangxi Agricultural University | Yu L.,Jiangxi Agricultural University | And 2 more authors.
Journal of Hazardous Materials | Year: 2010

A novel method was proposed to prepare amino starch by reacting ethylenediamine with previously synthesized dialdehyde starch. Different factors affecting the preparation, i.e., ethylenediamine concentration, ethylenediamine:dialdehyde starch molar ratio, pH, duration and temperature have been studied. The modified starch was characterized by IR spectroscopy, elemental analysis, X-ray diffraction and differential scanning calorimetry. The amino starch derivatives were evaluated as adsorbents. The adsorption activity for heavy metals such as Cu(II) and Cr(VI) by crosslinked amino starch was studied in terms of adsorption amount, kinetics and isotherm; and adsorbent reuse were also studied. Adsorption processes for Cu(II) and Cr(VI) on crosslinked amino starch fit a Langmuir isotherm, and adsorption for Cu(II) and Cr(VI) from aqueous solution was endothermic reactions. Crosslinked amino starch was very effective for the adsorption of Cu(II) and Cr(VI), and efficient in capacity, recycled. © 2010 Elsevier B.V.

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