Liang X.-X.,Jiangwan Hospital |
Li F.,Shanghai JiaoTong University |
Gao F.,Shanghai JiaoTong University |
Li C.-X.,Shanghai JiaoTong University |
And 3 more authors.
BioMed Research International | Year: 2016
This study was to explore the significance of ultrasound in determining whether the patients with secondary hyperparathyroidism (SHPT) are sensitive to calcitriol treatment. According to the decrease value of parathyroid hormone (PTH), 42 SHPT patients were divided into two groups: drug susceptible group and drug insusceptible group. These 42 SHPT patients' ultrasound images were retrospectively analyzed. The morphology, size, number, blood flow, elastic modulus, and perfusion of the parathyroid glands were correlated with drug therapeutic outcome (oral calcitriol). Most SHPT patients with drug susceptible showed volume <438.50 mm3 and number ≤2, with 0-1 structural and vascular patterns, associated with Relative Maximum Intensity (RIMAX) <1.59 and elastic modulus <18.8 kPa, whereas most SHPT patients with drug insusceptible showed volume ≥438.50 mm3 and number ≥3, with 2-3 structural and vascular patterns, associated with Relative Maximum Intensity (RIMAX) ≥1.59 and elastic modulus ≥18.8 kPa. Therefore, ultrasonography in SHPT allows an accurate definition of the morphology, size, number, blood flow, elastic modulus, and perfusion of the parathyroid glands and is useful in determining whether SHPT patients are sensitive to calcitriol treatment. © 2016 Xing-xin Liang et al.
Han F.-L.,Jiangwan Hospital |
Yu F.,Jiangwan Hospital |
Chen F.,Jiangwan Hospital
Journal of Clinical Neurology (China) | Year: 2012
Objective: To approach the change of serum C-reactive protein (CRP) level in patients with diabetes complicated acute cerebral infarction (DACI) and relationship between it and carotid plaque formation, hypertension. Methods: The serum CRP level was detected by immune scattering chromatometry in 103 cases of diabetes, 240 cases of acute cerebral infarction (ACI) and 130 cases of DACI. The carotid artery plaque was detected with color ultrasonic Doppler diagnostic apparatus. The hypertension history of all the patients was investigated. Results: The serum CRP le\el, ratio of bilateral carotid plaque, hypertension and course of hypertension in the DACI group and ACI group were significantly higher or longer than those in the diabetic group (P < 0.01-0.001). Thecourse of hypertension in DACI group was significantly shorter than ACI group(P < 0.05). Conclusions: The serum CRP level in patients with DACI is significantly higher than that in patients with diabetes. Bilateral carotid artery plaque formation and hypertension (especially long course) are main factors of DACI.
Ma L.S.,Tongji University |
Yan Q.,Jiangwan Hospital |
Huang Y.,Jiangwan Hospital |
Zhao W.,Jiangwan Hospital |
Zhu Y.,Jiangwan Hospital
Oncology Letters | Year: 2015
The aim of the current study was to investigate the suppressive effects of pSilencer T7-human epidermal growth factor receptor 2 (HER2)-short hairpin RNA (shRNA) recom-binant plasmids on human SKOV3 ovarian cancer cell growth and sensitivity to carboplatin (CBP). Three different pairs of shRNAs (shRNAa, shRNAb and shRNAc), targeting the HER2 gene, were selected and transfected into human SKOV3 cells, respectively. The expression levels of HER2 were then detected by immunohistochemical (IHC), semi-quantitative reverse transcription-polymerase chain reaction and western blot analyses. In addition, cell cycle and cell growth were investigated using cell counting kit-8 (CCK-8). The results of the IHC and western blot analyses revealed that shRNAb significantly inhibited HER2 protein expression in SKOV3 cells. shRNAb exhibited an improved effect on HER2 expres-sion compared with shRNAa (P<0.01), while shRNAc did not affect HER2 expression. Nontransfected and nonspecific shRNA groups were used as the negative controls. Knockdown of HER2 expression by shRNA was initiated at 24 h following transfection, achieving an optimum effect at 48 h and lasting for at least 72 h after the treatment. The CCK-8 cell growth assay indicated that the knockdown of HER2 expression in the SKOV3 cell line resulted in significant growth suppression and cell cycle arrest. In addition, inhibition of HER2 significantly increased SKOV3 cell sensitivity to CBP treatment. In conclu-sion, pSilencer T7-HER2-shRNA significantly inhibited HER2 expression in human ovarian cancer cells in vitro and induced chemotherapeutic sensitivity to CBP. © 2015, Spandidos Publications. All rights reserved.
Wang Y.,Jiangwan Hospital |
Song J.,Jiangwan Hospital |
Zhang J.,Jiangwan Hospital |
Qian C.,Shanghai University |
And 5 more authors.
Journal of Surgical Research | Year: 2013
Background Cell proliferation, renewal, and apoptosis factors are related to hemorrhagic shock (HS) survival. Objective Hepatic gene expression before and 24 h after induced HS were compared. Methods Male Sprague-Dawley rats aged 8-9 wk (n = 11) were subjected to blood loss, and HS was induced in 9 rats (blood loss <0.1 mL) by left lobular hepatectomy with fixed-volume blood loss (2.5 mL/100 g) for this self-controlled study. In 3 randomly selected rats surviving >24 h post-HS, hepatic tissue samples collected pre-HS (n = 3; group A) and 24 h post-HS (n = 3; group B) were used for microarray analysis (21,793 genes) of differentially expressed genes using pathway, gene ontology, and network analyses. Real-time reverse transcriptase polymerase chain reaction confirmed Aldh1a1, Aldh1a7, amine oxidase, copper containing 3, cytochrome P450 26A1, histidine decarboxylase 1, and epoxide hydrolase 2 expression using a beta-actin reference. Results Four rats survived 24 h after HS. Microarray revealed 562 upregulated and 634 downregulated genes in group A compared with group B. Gene ontology analysis revealed differentially expressed genes involved in cholesterol metabolic processes, extracellular stimuli response, sterol metabolic processes, hormonal stimuli response, steroid metabolic processes, endogenous stimulus response, oxidation and reduction reactions, organic substance response, and fatty acid metabolic processes. Conclusions HS pathogenesis involves numerous interrelated signaling pathways. Redox reaction and fatty acid metabolism pathway involvement in traumatic HS recovery, as well as other pathways, may provide novel targets for better understanding the pathology of HS and developing treatments to limit post-HS organ failure. © 2013 Elsevier Inc. All rights reserved.
Wang Y.,Jiangwan Hospital |
Zhao Z.,Jiangwan Hospital |
Bao X.,Jiangwan Hospital |
Fang Y.,Jiangwan Hospital |
And 4 more authors.
Medical Oncology | Year: 2014
Borealin/Dasra B, also called cell division cycle associated 8, is a member of the chromosomal passenger complex and plays a crucial role during mitosis and cell division. The expression of this gene has been investigated in several kinds of cancers, but its role in colorectal cancers (CRC) remains to be elucidated. In this study, our in silico analysis using the Oncomine database showed that Borealin was overexpressed in CRC tissues, which was further validated by immunohistochemistry in a cohort of 81 pairs of primary CRC and adjacent non-cancerous tissues. In CRC, Borealin expression was positively correlated with age (P = 0.0079), lymph node metastasis (P = 0.0086), and TNM stage (P = 0.0050). Significant difference in the overall survival time was seen between patients with high and low expression (P < 0.0001). Furthermore, high Borealin expression remained independently prognostic in multivariate analysis (HR 4.556, 95 % CI 1.964–10.567, P = 0.0004). Functional studies showed that suppression of Borealin expression with siRNA could significantly inhibit the growth and induce apoptosis in HCT-116 cells (P < 0.01). These results suggest that Borealin might act as an independent prognostic factor and a potential therapeutic target for patients with CRC. © 2014, Springer Science+Business Media New York.