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Zheng H.,Southern Medical University | Liu A.,Southern Medical University | Liu B.,Sun Yat Sen University | Li M.,Jiangsu Zeukov Pharmaceutical SandT. Inc | And 2 more authors.
Cancer Letters | Year: 2010

The purpose of this work is to study mechanisms underlying anti-tumor effects of farnesyltransferase inhibitors (FTIs) in non-small cell lung cancer (NSCLC). We demonstrate that mRNA and protein levels of Ras homologue enriched in brain (Rheb) are highly expressed both in NSCLC tissues and in NSCLC cell lines. Rheb expression levels correlate with phosphorylation of its downstream target S6 and the sensitivity of NSCLC cells to FTIs (R115777 and SCH66336)-induced growth inhibition and apoptosis. FTIs effectively and preferentially inhibited Rheb downstream signaling in NSCLC cells. Moreover, inhibition of Rheb functions by FTIs or dominant-negative Rheb mutants enhance the effects of cisplatin on NSCLC cells. Rheb-CSVL, a FTIs-resistant mutant, reduces the effects of FTIs on NSCLC cells. Our results suggest that Rheb is a critical target for FTIs therapy in NSCLC. © 2010 Elsevier Ireland Ltd. Source


Xie D.,Jiangsu Zeukov Pharmaceutical SandT. Inc | Xie D.,State Key Laboratory of Pharmaceutical New Technology for Chinese Medicine | Li Y.,Jiangsu Zeukov Pharmaceutical SandT. Inc | Li Y.,State Key Laboratory of Pharmaceutical New Technology for Chinese Medicine | And 13 more authors.
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To study the chemical constituents of the leaves of Naudea officinalis. Method: The chemical constituents were separated by column chromatography and semi-preparative HPLC techniques, and their structures were determined by spectral analysis. Result: Five compounds were isolated and identified as strictosamide(1), 10-hydroxy strictosamide(2), kaempferol-3-O-rutinoside(3), rutin (4), pumiloside(5). Conclusion: Among these compounds, 2, 3, 4 are isolated from the leaves of Naudea officinalis for the first time. Source


Xie D.-W.,Jiangsu Zeukov Pharmaceutical SandT. Inc | Li Y.-H.,Jiangsu Zeukov Pharmaceutical SandT. Inc | Li Y.-H.,Jiangsu Kanion Pharmaceutical Co. | Li Y.-H.,State Key Laboratory of New Technology for Chinese Medicine | And 12 more authors.
Journal of Food and Drug Analysis | Year: 2012

A simple, rapid and validated method was developed for the simultaneous determination of five characteristic components, including three alkaloids (pumiloside, 10-hydroxy strictosamide, and strictosamide), and two flavonoids (rutin and kaempferol-3-O-rutinoside), in the Nauclea officinalis leaves. The chromatographic separation was achieved with a lichrospher C-18 analytical column by gradient elution with (A) 0.1% aqueous phosphoric acid and (B) acetonitrile as mobile phase, with a flow rate of 1.0 mL/min. The column temperature was set at 30°C. Detection was carried out on a photodiode array detector at 226 nm. The method was validated for system suitability, precision, accuracy and linearity. The average recovery of the method was 98.00-100.44% and linearity (r) was>0.9993. Furthermore, the method was successfully applied to the simultaneous determination of five analytes in 10 samples of N. officinalis leaves from different harvesting time of the herb. The results suggested that the method could be efficiently employed to evaluate the quality of N. officinalis leaves from various sources, which would benefit its quality control. Source


Meng Z.-Q.,Jiangsu Zeukov Pharmaceutical SandT. Inc | Meng Z.-Q.,State Key Laboratory of New Technology for Chinese Medicine Pharmaceutical Process | Meng Z.-Q.,China Pharmaceutical University | Liu W.-J.,Jiangsu Zeukov Pharmaceutical SandT. Inc | And 13 more authors.
Chinese Journal of Natural Medicines | Year: 2013

Aim: To identify the structure of the acid-catalyzed product of strictosamide and explore the reaction mechanism. Methods: The acid-catalyzed reaction process of strictosamide was monitored by HPLC, and a macroporous resin was used to purify the reaction solution. The structure of the product was confirmed by MS, NMR, and ROESY spectra. Results: The acid-catalyzed transformation yield from strictosamide to vincoside lactam was 52%. Conclusion: The reaction mechanism of the transformation from strictosamide to vincoside lactam may be related to the stability of the three-dimensional configuration of the compound. These results offer a new way to obtain vincoside lactam from the widely distributed indole alkaloid strictosamide by acid-catalysis. © 2013 China Pharmaceutical University. Source


Meng Z.,Jiangsu Zeukov Pharmaceutical SandT. Inc | Meng Z.,Jiangsu Kanion Parmaceutical Co. | Meng Z.,State Key Laboratory of Pharmaceutical New Technology for Chinese Medicine | Ding G.,Jiangsu Zeukov Pharmaceutical SandT. Inc | And 14 more authors.
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To study on the purification of strictosamide from Nauclea officinalis by macroporous resin to provide reference for production. Method: The best macroporous resin was selected among 10 kinds of resins according to adsorption and desorption of the static adsorption experiments. The adsorption quantity, elution volume of water, concentration and elution volume of alcohol were determined according to the single factor experiment. Result: HPD400 was the best resin, and the best adsorption quantity was 20.23 mg·g -1, the elution volume of water and 30% alcohol was 6 BV, and the elution volume of 70% alcohol was 4 BV. Conclusion: This technology is simple, feasible, and it can provide reference for the industrialized production. Source

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