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Zhou G.,Nanjing University of Technology | Pang H.,Nanjing University of Technology | Tang Y.,Nanjing University of Technology | Yao X.,Nanjing University of Technology | And 12 more authors.
Food Chemistry | Year: 2014

In this study, a rapid, simple and sensitive analytical method was developed for the quantitative determination of 20 nucleosides and nucleobases in functional foods at trace levels using hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC-UPLC-TQ-MS/MS) in multiple-reaction monitoring (MRM) mode. Under optimised chromatographic conditions, good separation of 20 target compounds was achieved using a Waters Acquity UPLC BEH Amide column and gradient elution in 11 min. The limits of detection (LODs) and quantification (LOQs) were between 0.02-42.54 ng/mL and 0.05-98.18 ng/mL for the 20 analytes, respectively. This is the first report about simultaneous analysis of nucleosides and nucleobases in functional foods using this method, which afforded good linearity, precision, repeatability and accuracy. The method developed was successfully applied to quantify target compounds in batches of ginkgo seeds. The method potentially could be used to determine polar and trace-level nucleosides and nucleobases in ginkgo seeds. © 2013 Elsevier Ltd. All rights reserved. Source


Zhou G.,Nanjing University | Yao X.,Nanjing University | Tang Y.,Nanjing University | Qian D.,Nanjing University | And 2 more authors.
Journal of Plant Resources and Environment | Year: 2013

Eighteen compounds in petroleum, ethyl acetate and n-butanol fractions of ethanol crude extracts from mesosperm of Ginkgo biloba Linn. seed were isolated and obtained, they are stearic acid (I), palmitic acid (II), ginnol (III), sitosterol (IV), ndotriacontanol (V), n-docosanol (VI), hydroginkgolic acid (VII), ginkgolic acid (VIII), monobehenin (IX), glyceryl arachidate (X), 1-hexadecanoyl glyceride (XI), 1,3-dipalmitin (XII), 1,3-dilinolein (XIII), glycerol tristearate (XIV), glycerol tripalmitate (XV), ginkgolide B (XVI), ginkgolide C (XVII) and daucosterol (XVIII). In which, compounds V, VI, IX, X, XI, XII,XIII, XIVand XV are obtained firstly from G. biloba. Source


Zhou G.,Nanjing University | Yao X.,Nanjing University | Tang Y.,Nanjing University | Yang N.,Nanjing University | And 8 more authors.
Chemistry and Physics of Lipids | Year: 2012

Two new fatty alcohols named as (7S,8R,11S)-nonacosanetriol (1) and (10R,12R,15S)-nonacosanetriol (2), along with eight known compounds including ginkgolic acid (3), hydroginkgolic acid (4), sciadopitysin (5), ginkgetin (6), isoginkgetin (7), ginkgolide A (8), ginkgolide B (9) and ginkgolide C (10) have been isolated from the petroleum ether extract of Ginkgo biloba sarcotesta. Their structures were elucidated by means of chemical and extensive spectroscopic analysis. The absolute stereochemistry of compounds 1 and 2 was elucidated on the spectroscopic analysis of the R- and S-MTPA esters. Compounds 1 and 2 exhibited slight activity of antithrombin and moderate activity of antiplatelet aggregation in vitro. This was the first report regarding the anticoagulative activities of biflavonoids in G. biloba, and isoginkgetin (7) showed significant antithrombin and antiplatelet aggregation activity. © 2012 Elsevier Ireland Ltd. All rights reserved. Source


Yao X.,Nanjing University | Shang E.,Nanjing University | Zhou G.,Nanjing University | Tang Y.,Nanjing University | And 6 more authors.
International Journal of Molecular Sciences | Year: 2012

The extract from Ginkgo biloba leaves has become a very popular plant medicine and herbal supplement for its potential benefit in alleviating symptoms associated with peripheral vascular disease, dementia, asthma and tinnitus. Most research on G. biloba leaves focus on the leaves collected in July and August from four to seven year-old trees, however a large number of leaves from fruit cultivars (trees older than 10 years) are ignored and become obsolete after fruit harvest season (November). In this paper, we expand the tree age range (from one to 300 years) and first comparatively analyze the total flavonol glycosides and terpene lactones at different ages, from different cultivation sources and genders of G. biloba leaves collected in November by using the validated HPLC-ELSD and HPLC-PDA methods. The results show that the contents of total terpene lactones and flavonol glycosides in the leaves of young ginkgo trees are higher than those in old trees, and they are higher in male trees than in female trees. Geographical factors appear to have a significant influence on the contents as well. These results will provide a good basis for the comprehensive utilization of G. biloba leaves, especially the leaves from fruit cultivars. © 2012 by the authors; licensee MDPI, Basel, Switzerland. Source


Zhou G.,Nanjing University | Yao X.,Nanjing University | Tang Y.,Nanjing University | Qian D.,Nanjing University | And 5 more authors.
Food Chemistry | Year: 2014

An optimized method of ultrasound-assisted extraction followed by ultra-high-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (UAE-UHPLC-TQ/MS2) was proposed for the simultaneous extraction and determination of 26 characteristic components covering four structure types (flavonoids, terpene lactones, ginkgolic acids and phenylpropanols) in ginkgo seeds (GSs). The UAE parameters (ultrasound power, time and solvent-to-material ratio) were optimized using a response surface methodology. This is the first report of the simultaneous analysis of 26 compounds in Ginkgo biloba using UHPLC-TQ/MS2; this analysis afforded good linearity, precision, repeatability and accuracy. UAE-UHPLC-TQ/MS 2 was successfully applied to ginkgo seed samples, and the analysis showed that GSs are rich in terpene lactones and could be selected as a healthy food resource. The results suggest that UAE-UHPLC-TQ/MS2 might be able to be utilized as a tool for the quality assessment of samples from GSs or other related products using flavonoids, terpene lactones, ginkgolic acids and phenylpropanols as markers. © 2014 Elsevier Ltd. All rights reserved. Source

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