Zhang J.,Beijing Forestry University |
Zhang J.,Jiangsu Riverine Institute of Agricultural science |
Yuan H.,Beijing Forestry University |
Li M.,Jiangsu Riverine Institute of Agricultural science |
And 7 more authors.
PLoS ONE | Year: 2016
As a salt-tolerant arbor tree species, Salix matsudana plays an important role in afforestation and greening in the coastal areas of China. To select superior Salix varieties that adapt to wide saline areas, it is of paramount importance to understand and identify the mechanisms of salt-tolerance at the level of the whole genome. Here, we describe a high-density genetic linkage map of S. matsudana that represents a good coverage of the Salix genome. An intraspecific F1 hybrid population was established by crossing the salt-sensitive "Yanjiang" variety as the female parent with the salt-tolerant "9901" variety as the male parent. This population, along with its parents, was genotyped by specific length amplified fragment sequencing (SLAF-seq), leading to 277,333 high-quality SLAF markers. By marker analysis, we found that both the parents and offspring were tetraploid. The mean sequencing depth was 53.20-fold for "Yanjiang", 47.41-fold for "9901", and 11.02-fold for the offspring. Of the SLAF markers detected, 42,321 are polymorphic with sufficient quality for map construction. The final genetic map was constructed using 6,737 SLAF markers, covering 38 linkage groups (LGs). The genetic map spanned 5,497.45 cM in length, with an average distance of 0.82 cM. As a first high-density genetic map of S. matsudana constructed from salt tolerance-varying varieties, this study will provide a foundation for mapping quantitative trait loci that modulate salt tolerance and resistance in Salix and provide important references for molecular breeding of this important forest tree. © This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Wang K.,Jiangsu Riverine Institute of Agricultural science |
He L.,Jiangsu Riverine Institute of Agricultural science |
Yan H.,Jiangsu Riverine Institute of Agricultural science |
Wei X.,Jiangsu Riverine Institute of Agricultural science
Israel Journal of Plant Sciences | Year: 2015
Polyploidy induction is an important method for the innovation of germplasm resources because polyploid plants have superior characteristics to diploid ones. In this paper, oriental melon (Cucumis melo var. makuwa) tetraploids were induced by treating the apical meristem of seedlings with oryzalin, amiprophos-methyl (APM) and colchicine. The results showed that each of these three antimicrotubule agents could induce the formation of tetraploid oriental melon, but oryzalin and APM showed higher efficiencies of tetraploid induction. The efficiencies of tetraploid induction were highest (14.44%) when treated with 80 μmol·L-1 APM for 6 days. Stomata characteristics were used to screen the treated plants for changes in ploid levels preliminarily. Subsequently, the changes in ploid levels were further confirmed by flow-cytometric analysis and chromosome counts of root tip cells. The comparison between diploids and tetraploids in fruit characteristics, reproductive characteristics, and postharvest storage qualities showed that the fruit of tetraploids had thicker flesh, higher total soluble solid (TSS) content and lower fruit shape index than diploids. The pollen grains of tetraploids were larger but showed lower vitality. In addition, a higher percentage of embryo-deficient seeds contributed to lower fertility. During postharvest storage, the fruit of tetraploids softened faster, had higher TSS content and lower respiration rate. © 2015 Jiangsu Riverine Institute of Agricultural Science.