Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Co Innovation Center for Modern Production Technology of Grain Crops

Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Co Innovation Center for Modern Production Technology of Grain Crops

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Li T.,Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Co Innovation Center for Modern Production Technology of Grain Crops | Zhang D.,Kansas State University | Zhou X.,Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Co Innovation Center for Modern Production Technology of Grain Crops | Bai G.,Kansas State University | And 3 more authors.
Euphytica | Year: 2015

To determine if Chinese and Japanese wheat landraces and varieties have unique sources of Fusarium head blight (FHB) resistance, an association mapping panel of 195 wheat accessions including both commercial varieties and landraces was genotyped with 364 genome-wide simple sequence repeat and sequence-tagged site (STS) markers, and evaluated for type II FHB resistance in three greenhouse experiments using single floret inoculation. Population structure analysis stratified this population into five groups with Chinese landraces in four groups. Thirty-two of 51 Chinese landraces and 24 of 27 Japanese accessions were placed in one group. Association analysis using a mixed model identified 11 markers having significant associations with FHB resistance in at least two experiments. Most of these markers coincided with known quantitative trait loci (QTL) for FHB resistance with one potentially novel QTL associated with Xgdm138-5DS and Xgwm358-5DS. Xbarc19-3AS was significant in all three experiments, and the frequency of favorable alleles was more than 53 %. Chinese landraces and Japanese accessions had more favorable alleles at the majority of reproducible marker loci. Nine QTL combinations were identified according to the number of favorable alleles. Mean FHB severities increased with decreasing numbers of favorable alleles at reproducible loci. The resistance loci characterized here will further diversify the wheat FHB resistance gene pool, and provide breeders with additional sources of resistance for improvement of FHB resistance in wheat. © 2015 Springer Science+Business Media Dordrecht


Li L.,Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Co Innovation Center for Modern Production Technology of Grain Crops | Shi X.,Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Co Innovation Center for Modern Production Technology of Grain Crops | Zheng F.,Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Co Innovation Center for Modern Production Technology of Grain Crops | Li C.,Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Co Innovation Center for Modern Production Technology of Grain Crops | And 6 more authors.
Theoretical and Applied Genetics | Year: 2016

Key message: Using bulk segregant analysis (BSA) coupling with RNA-seq and DNA markers identified a potentially novel nitrogen-dependent lesion mimic geneNdhrl1on 2BS in wheat.Abstract: Lesion mimic (LM) refers to hypersensitive reaction-like (HRL) traits that appear on leaf tissue in the absence of plant pathogens. In a wheat line P7001, LM showed up on the leaves under the 0 g nitrogen (N) treatment, but disappeared when sufficient N was supplied, suggesting that LM is N-responsive and N dosage dependent. Using BSA strategy together with RNA-seq and DNA markers, we identified an N-dependent LM gene (Ndhrl1) and mapped it to the short arm of chromosome 2B using an F5 recombinant inbred population developed from the cross of P7001 × P216. The putative gene was delimited into an interval of 8.1 cM flanked by the CAPS/dCAPS markers 7hrC9 and 7hr2dc14, and co-segregated with the dCAPS marker 7hrdc2. This gene is most likely a novel gene for LM in wheat based on its chromosomal location. Further analysis of RNA-seq data showed that plant–pathogen interaction, nitrogen metabolism, zeatin biosynthesis and plant hormone signal transduction pathways were significantly differentially expressed between LM and non-LM lines. © 2016 Springer-Verlag Berlin Heidelberg

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