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Duan Z.-Q.,Academy of State Administration of GrainBeijing | Fang X.-L.,Academy of State Administration of GrainBeijing | Wang Z.-Y.,Huaiyin Institute of Technology | Wang Z.-Y.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration | And 2 more authors.
ACS Sustainable Chemistry and Engineering | Year: 2015

1,3-Diacylglycerol is conducive to reducing the increase of body fat and slowing the raise of body weight. So, growing attention has been paid to the enzyme-mediated 1,3-diacylglycerol preparation due to the excellent selectivity and the environmental friendliness. In this work, we provided an alternative and sustainable process for 1,3-diacylglycerol synthesis catalyzed by immobilized lipase from Penicillium expansum (PEL) in which the esterification of oleic acid with glycerol for 1,3-diolein synthesis was employed as the model reaction. The results indicated that, under the optimum conditions (10.0 g of oleic acid, 1.09 g of glycerol, 80 U of immobilized PEL, and 6.4 g of 4 Å molecular sieves at 40 °C for 12 h), the diolein yield achieved to 82.4%, and the value of 1,3-diolien/diolein was 87.3%. Additionally, 92% original activity of the immobilized PEL was maintained after being used for 10 batches, highlighting the presumable cost-effectiveness of the enzyme. This study could be considered as an alternative and green strategy for preparing 1,3-diacylglycerol. © 2015 American Chemical Society. Source

Bi Y.,Huaiyin Institute of Technology | Du W.,Huaiyin Institute of Technology | Wang Z.,Huaiyin Institute of Technology | Wang Z.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration | And 3 more authors.
Asian Journal of Chemistry | Year: 2014

In this paper, the regioselective acylation of 6-azauridine for preparing its potential prodrugs was successfully conducted in the hydrophobicity-based binary solvent system using Candida antarctica lipase B. Comparative study showed that the reaction rate, thermal and operational stability of the enzyme were dramatically improved by using the binary mixtures of acetone and isooctane (85:15, v/v as the reaction medium. In addition, the acyl donor sutructure displayed great influence on the behaviour of the enzyme in binary solvent system, presumably due to their different interactions with the active site of the lipase. Source

Bi Y.-H.,Huaiyin Institute of Technology | Wang Z.-Y.,Huaiyin Institute of Technology | Wang Z.-Y.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration | Duan Z.-Q.,Academy of State Administration of Grain | And 3 more authors.
RSC Advances | Year: 2015

The solvent effect on the positional selectivity of the immobilized lipase from Burkholderia cepacia in 1,3-diolein synthesis was investigated for the first time. The results indicated that the preferential selectivity to sn-1 hydroxyl of the glycerol molecule over sn-2 hydroxyl was weaker in solvents with higher logP values. © 2015 The Royal Society of Chemistry. Source

Gou Y.,Huaiyin Institute of Technology | Zhou R.,Huaiyin Institute of Technology | Ye X.,Huaiyin Institute of Technology | Gao S.,Huaiyin Institute of Technology | And 2 more authors.
Science and Technology of Advanced Materials | Year: 2015

Silver nanoparticles (AgNPs) have been widely used in diverse fields due to their superior properties. Currently the biosynthesis of AgNPs is in the limelight of modern nanotechnology because of its green properties. However, relatively low yield and inefficiency diminish the prospect of applying these biosynthesized AgNPs. In this work, a rapid mass AgNP biosynthesis method using the cell-free extract of a novel bacterial strain, Lysinibacillus sphaericus MR-1, which has been isolated from a chemical fertilizer plant, is reported. In addition, the optimum synthesis conditions of AgNPs were investigated. The optimum pH, temperature, dosage, and reaction time were 12, 70°C, 20 mM AgNO3, and 75 min, respectively. Finally, AgNPs were characterized by optical absorption spectroscopy, zeta potential and size distribution analysis, x-ray diffraction, electron microscopy, and energy-dispersive x-ray spectroscopy. The results revealed that these biosynthesized AgNPs were bimolecular covered, stable, well-dispersed face centered cubic (fcc) spherical crystalline particles with diameters in the range 5-20 nm. The advantages of this approach are its simplicity, high efficiency, and eco-friendly and cost-effective features. © 2015 National Institute for Materials Science. Source

Bi Y.-H.,Huaiyin Institute of Technology | Duan Z.-Q.,Academy of State Administration of Grain | Du W.-Y.,Huaiyin Institute of Technology | Wang Z.-Y.,Huaiyin Institute of Technology | Wang Z.-Y.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration
Biotechnology Letters | Year: 2015

Purpose of work: The bio-based solvents limonene and p-cymene obtained from citrus waste were innovatively employed as the reaction media for enzymatic synthesis of phosphatidylserine. (R)-(+)-Limonene, which is available in large quantities from citrus waste, and its close derivative p-cymene, are promising green solvents. Herein, they were successfully employed as reaction media for enzyme-mediated transphosphatidylation of phosphatidylcholine with l-serine for phosphatidylserine synthesis for the first time. A 95 % yield of phosphatidylserine was achieved after 12 h and the side-reactions (which are the undesirable hydrolysis of phosphatidylcholine and phosphatidylserine) did not happen. This work presents an alternative strategy for preparing phosphatidylserine that possesses obvious advantages over the traditional processes in terms of high efficiency combined with environmental friendliness. © 2014, Springer Science+Business Media Dordrecht. Source

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