Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration

Huaian, China

Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration

Huaian, China
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Wang Z.-Y.,Huaiyin Institute of Technology | Wang Z.-Y.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration | Bi Y.-H.,Huaiyin Institute of Technology | Yang R.-L.,Huaiyin Institute of Technology | And 6 more authors.
Journal of Biotechnology | Year: 2015

In this work, comparative experiments were explored to investigate the substrate specificity of Pseudomonas cepacia lipase in regioselective acylation of nucleosides carrying various substituents (such as the H, F, Cl, Br, I) at 2'- and 5-positions. Experimental data indicated that the catalytic performance of the enzyme depended very much on the halo-substituents in nucleosides. The increased bulk of 2'-substituents in ribose moiety of the nucleoside might contribute to the improved 3'-regioselectivity (90-98%, nucleosides a- d) in enzymatic decanoylation, while the enhancement of regioselectivity (93-99%) in 3'- O-acylated nucleosides e- h could be attributable to the increasing hydrophobicity of the halogen atoms at 5-positions. With regard to the chain-length selectivity, P. cepacia lipase displayed the highest 3'-regioselectivity toward the longer chain (C14) as compared to shorter (C6 and C10) ones. The position, orientation and property of the substituent, specific structure of the lipase's active site, and acyl structure could account for the diverse results. © 2015.


Bi Y.,Huaiyin Institute of Technology | Wang Z.,Huaiyin Institute of Technology | Wang Z.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration | Nie L.,Huaiyin Institute of Technology | And 2 more authors.
Asian Journal of Chemistry | Year: 2013

Organic co-solvents can improve the catalytic power, activity and stability of the enzyme. In this paper, regioselective transesterification of 6-azauridine with vinyl palmitate using immobilized lipase from Thermomyces lanuginosus in the co-solvent system was described. The optimum conditions were screened out. The optimal volumetric ratio of the co-solvent mixture, molar ratio of 6-azauridine to vinyl palmitate and reaction temperature were found to be acetone/isooctane (90:10 v/v), 1:5 and 50 °C, under which the reaction rate, substrate conversion and 5'-regioselectivity were 20.6 mM/h, >99.0 and 99.0 %, respectively. Moreover, the thermal stability and operational stability of the enzyme were enhanced expressly by changing the reaction solvent from pure acetone to a mixture of acetone and isooctane.


Bi Y.,Huaiyin Institute of Technology | Du W.,Huaiyin Institute of Technology | Wang Z.,Huaiyin Institute of Technology | Wang Z.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration | And 3 more authors.
Asian Journal of Chemistry | Year: 2014

In this paper, the regioselective acylation of 6-azauridine for preparing its potential prodrugs was successfully conducted in the hydrophobicity-based binary solvent system using Candida antarctica lipase B. Comparative study showed that the reaction rate, thermal and operational stability of the enzyme were dramatically improved by using the binary mixtures of acetone and isooctane (85:15, v/v as the reaction medium. In addition, the acyl donor sutructure displayed great influence on the behaviour of the enzyme in binary solvent system, presumably due to their different interactions with the active site of the lipase.


Duan Z.-Q.,Academy of State Administration of GrainBeijing | Fang X.-L.,Academy of State Administration of GrainBeijing | Wang Z.-Y.,Huaiyin Institute of Technology | Wang Z.-Y.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration | And 2 more authors.
ACS Sustainable Chemistry and Engineering | Year: 2015

1,3-Diacylglycerol is conducive to reducing the increase of body fat and slowing the raise of body weight. So, growing attention has been paid to the enzyme-mediated 1,3-diacylglycerol preparation due to the excellent selectivity and the environmental friendliness. In this work, we provided an alternative and sustainable process for 1,3-diacylglycerol synthesis catalyzed by immobilized lipase from Penicillium expansum (PEL) in which the esterification of oleic acid with glycerol for 1,3-diolein synthesis was employed as the model reaction. The results indicated that, under the optimum conditions (10.0 g of oleic acid, 1.09 g of glycerol, 80 U of immobilized PEL, and 6.4 g of 4 Å molecular sieves at 40 °C for 12 h), the diolein yield achieved to 82.4%, and the value of 1,3-diolien/diolein was 87.3%. Additionally, 92% original activity of the immobilized PEL was maintained after being used for 10 batches, highlighting the presumable cost-effectiveness of the enzyme. This study could be considered as an alternative and green strategy for preparing 1,3-diacylglycerol. © 2015 American Chemical Society.


Bi Y.-H.,Huaiyin Institute of Technology | Du W.-Y.,Huaiyin Institute of Technology | Wang Z.-Y.,Huaiyin Institute of Technology | Wang Z.-Y.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration | And 3 more authors.
Applied Biochemistry and Biotechnology | Year: 2014

The effect of 2′-differing substituent groups (such as the H, F, Cl, Br, OCH3) as well as various chain lengths of the acyl substrates (C6, C10, and C14) on the performance of Thermomyces lanuginosus lipase for the regioselective acylation of pyrimidine nucleoside analogs was investigated systematically for the first time. The results evidently demonstrated that changing substituents in nucleosides had a significant influence on the substrate specificity for the enzymatic reaction, possibly due to the differences in physicochemical properties of the substituents and the special shape of the substrate binding site in enzyme active region. With respect to the chain-length selectivity, the enzyme exhibited the highest 5′- regioselectivity toward the longer chain (C14) as compared to short (C6 and C10) ones. The reason might be attributed to the presence of the interaction between the acyl chain and the large hydrophobic substrate-binding pocket. © 2014 Springer Science+Business Media.


Bi Y.-H.,Huaiyin Institute of Technology | Duan Z.-Q.,Academy of State Administration of Grain | Li X.-Q.,Huaiyin Institute of Technology | Li X.-Q.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration | And 3 more authors.
Journal of Agricultural and Food Chemistry | Year: 2015

Biobased ionic liquids with cholinium as the cation and amino acids as the anions, which could be prepared from renewable biomaterials by simple neutralization reactions, have recently been described as promising and green solvents. Herein, they were successfully used as the reaction media for enzyme-mediated transphosphatidylation of phosphatidylcholine with l-serine for phosphatidylserine synthesis for the first time. Our results indicated that the highest phosphatidylserine yield of 86.5% was achieved. Moreover, 75% original activity of the enzyme was maintained after being used for 10 batches. The present work could be considered an alternative enzymatic strategy for preparing phosphatidylserine. Additionally, the excellent results make the biobased ionic liquids more promising candidates for use as environmentally friendly solvents in biocatalysis fields. © 2015 American Chemical Society.


Bi Y.-H.,Huaiyin Institute of Technology | Wang Z.-Y.,Huaiyin Institute of Technology | Wang Z.-Y.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration | Duan Z.-Q.,Academy of State Administration of Grain | And 3 more authors.
RSC Advances | Year: 2015

The solvent effect on the positional selectivity of the immobilized lipase from Burkholderia cepacia in 1,3-diolein synthesis was investigated for the first time. The results indicated that the preferential selectivity to sn-1 hydroxyl of the glycerol molecule over sn-2 hydroxyl was weaker in solvents with higher logP values. © 2015 The Royal Society of Chemistry.


You Q.,Huaiyin Institute of Technology | You Q.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration | You Q.,Nanjing University | Yin X.,Huaiyin Institute of Technology | And 3 more authors.
Carbohydrate Polymers | Year: 2014

Four methods for extracting polysaccharides from Boletus edulis, namely, hot-water extraction, ultrasonic clearer extraction, static probe ultrasonic extraction, and pulsed counter-current probe ultrasonic extraction (CCPUE), were studied. Results showed that CCPUE has the highest extraction efficiency among the methods studied. Under optimal CCPUE conditions, a B. edulis polysaccharide (BEP) yield of 8.21% was obtained. Three purified fractions, BEP-I, BEP-II, and BEP-III, were obtained through sequential purification by DEAE-52 and Sephadex G-75 chromatography. The average molecular weights of BEP-I, BEP-II, and BEP-III were 10,278, 23,761, and 42,736 Da, respectively. The polysaccharides were mainly composed of xylose, mannose, galactose, and glucose; of these, mannose contents were the highest. The antioxidant activities of the BEPs were further investigated by measurement of their ability to scavenge DPPH and hydroxyl radicals as well as their reducing power. The results indicated that the BEPs have good antioxidant activity. © 2013 Elsevier Ltd. All rights reserved.


Gou Y.,Huaiyin Institute of Technology | Zhou R.,Huaiyin Institute of Technology | Ye X.,Huaiyin Institute of Technology | Gao S.,Huaiyin Institute of Technology | And 2 more authors.
Science and Technology of Advanced Materials | Year: 2015

Silver nanoparticles (AgNPs) have been widely used in diverse fields due to their superior properties. Currently the biosynthesis of AgNPs is in the limelight of modern nanotechnology because of its green properties. However, relatively low yield and inefficiency diminish the prospect of applying these biosynthesized AgNPs. In this work, a rapid mass AgNP biosynthesis method using the cell-free extract of a novel bacterial strain, Lysinibacillus sphaericus MR-1, which has been isolated from a chemical fertilizer plant, is reported. In addition, the optimum synthesis conditions of AgNPs were investigated. The optimum pH, temperature, dosage, and reaction time were 12, 70°C, 20 mM AgNO3, and 75 min, respectively. Finally, AgNPs were characterized by optical absorption spectroscopy, zeta potential and size distribution analysis, x-ray diffraction, electron microscopy, and energy-dispersive x-ray spectroscopy. The results revealed that these biosynthesized AgNPs were bimolecular covered, stable, well-dispersed face centered cubic (fcc) spherical crystalline particles with diameters in the range 5-20 nm. The advantages of this approach are its simplicity, high efficiency, and eco-friendly and cost-effective features. © 2015 National Institute for Materials Science.


Bi Y.-H.,Huaiyin Institute of Technology | Duan Z.-Q.,Academy of State Administration of Grain | Du W.-Y.,Huaiyin Institute of Technology | Wang Z.-Y.,Huaiyin Institute of Technology | Wang Z.-Y.,Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration
Biotechnology Letters | Year: 2015

Purpose of work: The bio-based solvents limonene and p-cymene obtained from citrus waste were innovatively employed as the reaction media for enzymatic synthesis of phosphatidylserine. (R)-(+)-Limonene, which is available in large quantities from citrus waste, and its close derivative p-cymene, are promising green solvents. Herein, they were successfully employed as reaction media for enzyme-mediated transphosphatidylation of phosphatidylcholine with l-serine for phosphatidylserine synthesis for the first time. A 95 % yield of phosphatidylserine was achieved after 12 h and the side-reactions (which are the undesirable hydrolysis of phosphatidylcholine and phosphatidylserine) did not happen. This work presents an alternative strategy for preparing phosphatidylserine that possesses obvious advantages over the traditional processes in terms of high efficiency combined with environmental friendliness. © 2014, Springer Science+Business Media Dordrecht.

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