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Jin P.,Jiangsu Provincial Academy of Traditional Chinese Medicine
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica | Year: 2012

To study the effects of triterpenoid components from Prunella asiatica on phase II detoxifying enzymes and protein expression in vitro and in vivo. Normal human bronchial epithelial (NHBE) cell model was used in vitro, and the mouse model of Kunming (KM) mice was used in vivo. CDNB assay was used to measure the activity of GST. NADPH and DCIP was used to detect the activity of NQO1. DTNB colorimetric assay was used to detect GSH. Western blot was use to detect the protein expression of NQO1. We found that triterpenoid components from P. asiatica could increase the activity of GST, NQO1 and GSH in NHBE cells and KM mice. NQO1 protein expression can also be increased in vitro. The study suggests that triterpenoid components from P. asiatica can prevent the lung cancer by regulating the body phase II detoxification enzyme activity and protein expression.

Shao H.,Shenyang University | Ni Y.,Catholic University of Leuven | Zhang J.,Jiangsu Provincial Academy of Traditional Chinese Medicine | Chen F.,Catholic University of Leuven | And 4 more authors.
PLoS ONE | Year: 2013

Evaluation of vascular disrupting treatment (VDT) is generally based on tumor size and enhancement on conventional magnetic resonance imaging (MRI) which, unfortunately, may be limited in providing satisfactory information. The purpose of the study is to evaluate consecutive changes of 20 rabbit VX2 liver tumors after VDT by dynamic contrast-enhanced MRI (DCE-MRI) and diffusion-weighted imaging (DWI) at a 3.0 T MR unit. Twenty four hours after intravenous injection of Combretastatin A-4-phosphate (CA4P) at 20 mg/kg, DCE-MRI derived Maximum Slope of Increase (MSI) and Positive Enhancement Integral (PEI) decreased sharply due to sudden shutting down of tumor feeding vessels. DWI derived Apparent Diffusion Coefficient (ADC) in tumor periphery decreased because of ischemic cell edema. On day 4, an increase of MSI was probably caused by the recovery of blood supply. A remarkable increase of ADC represented a large scale of necrosis among tumors. On day 8, the blood perfusion further decreased and the extent of necrosis further increased, reflected by lower MSI and PEI values and higher ADC value. On day 12, a second decrease of ADC was noticed because the re-growth of periphery tumor. The experimental data indicate that the therapeutic effects of VDT may be noninvasively monitored with DCE-MRI (reflecting tumor blood perfusion) and DWI (reflecting the changes of histology), which provide powerful measures for assessment of anticancer treatments. © 2013 Shao et al.

Wang P.J.,Jiangsu Provincial Academy of Traditional Chinese Medicine
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2011

To study the effect o f Bushen Huoxue Decoction (BHD) on neurobiochemical markers in the hippocampus of female rats with repeated immobilization stress. Sixty female rats were randomly divided into the normal group, the model group, the positive control group (treated with Liuwei Dihuang Pill at the dose of 3.3 g crude drug/kg), and the high, middle, and low BHD treated groups (at the dose of 8, 4, 2 g crude drug/kg), ten in each group. Chronic psychological stress was induced using repeated immobilization stress in rats. Medication was conducted by gastrogavage while modeling once a day for twenty successive days. The hippocampal neurohumoral levels were detected with high-performance liquid chromatography. The expression levels of BDNF and its receptor in the hippocampus were detected by Westem blot. Effect of BHD on neurobiochemical markers in the hippocampus of rats with repeated immobilization stress was observed. The levels of Glu, GABA, and BDNF in the hippocampus of the normal group were 1280.0 +/- 258.3 ng/mg, 588.3 +/- 115.1 ng/mg, and 13.26 +/- 2.57 gray value, respectively. But the hippocampal neurohumoral levels and the expression of BDNF in the model group obviously decreased when compared with the normal group, being 1016.9 +/- 215.9 ng/mg, 485.1 +/- 71.0 ng/mg, and 7.23 +/- 0.61 gray value, respectively. The levels of Glu (ng/mg) in hippocampus of the three BHD treated groups were 1459.1 +/- 413.5, 1894.7 +/- 542.8, and 1373.3 +/- 345.7, respectively. GABA levels (ng/mg) inthe hippocampus were 631.6 +/- 161.4, 899.1 +/- 262.1, and 656.4 +/- 140.8, respectively. BDNF levels (gray value) were 16.57 +/- 1.52, 29.85 +/- 1.37, and 24.44 +/- 3.81, respectively, significantly higher than that of the model group (P<0.05, P<0.01). The level of Glu in the positive control group (1216.5 +/- 193.8 ng/mg) was significantly higher than that of model group (P<0.05). BHD showed significant accommodation on the hippocampal neurohumoral levels and the expression of BDNF in the female rats with repeated immobilization stress.

Yan Y.,China Pharmaceutical University | Chai C.-Z.,China Pharmaceutical University | Wang D.-W.,Jiangsu Provincial Academy of Traditional Chinese Medicine | Yue X.-Y.,China Pharmaceutical University | And 2 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2013

Ge-Gen Decoction (GGD) is a classical formula of traditional Chinese medicine. It is generally used for treating common cold, fever and influenza in China and South East Asia. In this study, a systematic method was established for the qualitative and quantitative analysis of the major constituents in GGD. For qualitative analysis, a method of liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) was developed for identification of multi-constituents. Based on the UV spectra, retention time and MS spectra, sixty compounds in GGD extract were identified or tentatively characterized by comparing with reference substances or literatures. According to the qualitative results, a new quantitative analysis method of GGD was established by HPLC-DAD. Fourteen representative compounds unequivocally identified were chosen as marker components which were derived from five herbs in GGD excluding Zingiberis Rhizoma Recens and Jujubae Fructus. The analytical method was validated through intra- and inter-day precision, repeatability and stability, and the R.S.D. was less than 3.18%, 4.48%, 3.36% and 3.54%, respectively. The LODs and the LOQs for the analytes were less than 1.06 and 3.12μgmL-1, respectively. The overall recoveries ranged from 94.8% to 105.6%, with the R.S.D. ranging from 0.68% to 3.23%. Then the new method was applied to determine twelve batches of GGD commercial products of three dosage forms. The results indicated that the new approach was applicable in the routine analysis and quality control of GGD products. The study might provide a basis for quality control of GGD, and further study of GGD in vivo. © 2013 Elsevier B.V.

Ren A.-N.,Jiangsu Provincial Academy of Traditional Chinese Medicine | Lu Y.,Nanjing University | Zou Y.-F.,Jiangsu University | Shen H.,Jiangsu Provincial Academy of Traditional Chinese Medicine
European Food Research and Technology | Year: 2013

The crude water-soluble polysaccharide (SCP) was extracted from safflower by using hot water, and further fractioned by DEAE-52 anion exchange and Sephadex G-100 gel column chromatography to yield two acidic polysaccharides (SCP2 and SCP3). High-performance gel permeation chromatograph analysis showed that molecular weights (M W) of polysaccharides (SCP2 and SCP3) were 5, 860 and 9,330 Da, respectively. According to Fourier transform infrared spectroscopy, gas chromatography and chemical methods, SCP2 consisted of rhamnose, arabinose, glucose and galactose with a molar ratio of 2.93:11.19:33.68:3.48, while the primary sugar residues were (1 → 2)-linked-rhamnopyranose, (1 → 2)-linked and (1 → 3)-linked-arabinofuranose, (1 → 3)-linked-glucopyranose and (1 → 2)-linked or (1 → 6)-linked-galactopyranose. Likewise, SCP3 was composed of rhamnose, arabinose, xylose, mannose, glucose and galactose in a molar ratio of 4.44:1.46:4.51:5.82:8.23:19.38, while the primary sugar residues were (1 → 2)-linked-rhamnopyranose, (1 → 2)-linked-xylofuranose, (1 → 3)-linked-arabinofuranose, (1 → 2)-linked or (1 → 6)-linked-mannopyranose and glucopyranose, (1 → 2)-linked or (1 → 6)-linked or (1 → 3)-linked-galactopyranose. © 2013 Springer-Verlag Berlin Heidelberg.

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