Jiangsu Provincial Academy of Traditional Chinese Medicine

Nanjing, China

Jiangsu Provincial Academy of Traditional Chinese Medicine

Nanjing, China
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Jing X.-Y.,Nanjing University | Peng Y.-R.,Jiangsu Provincial Academy of Traditional Chinese Medicine | Wang X.-M.,Nanjing University | Duan J.-A.,Nanjing University
Chinese Pharmacological Bulletin | Year: 2015

Aim: To study the combined effect Euphorbia kansui of and Glycyrrhiza uralensis on metabolism of Kansuinine A and Kansuinine B. Methods: Control, GU and GU plus EK groups were treated for 10 days, respectively. Then the liver microsomes were prepared. KA and KB were incubated with microsomes of each group, and concentrations of KA and KB were measured to reflect the metablism of KA and KB. Erythromycin, diphenhydramine hydrochloride, benzbromarone, Cimetidine, fluconazole the inhibitors of CYP3A4, CYP2D6, CYP2C9, CYP1A2, CYP2C19 respectively, were incubated with KA and KB. Concentrations of KA and KB were measured to reveal the cytochrome P450 isoforms which involved in the metabolism of them. KA and KB were incubated with glycyrrhizic acid and enoxolone. Then concentrations of KA and KB were measured to reveal the effects of glycyrrhizic acid and enoxolone to KA and KB. Results: Concentrations of KA and KB in GU plus EK group were significantly higher than those in control and EK groups, which showed that the metablisom of KA and KB was inhibited in GU plus EK group. In addition, concentrations of KA and KB were higher in microsomes incubated with erythromycin, diphenhydramine hydrochloride, benzbromarone, Cimetidine and fluconazole than in control group, which revealed that the metablism of KA and KB was slowed down when CYP3A4, CYP2D6, CYP2C9, CYP1A2 and CYP2C19 were inhibited. They may participate in the metablisom of KA and KB. After incubation with glycyrrhizic acid and enoxolone, the metablism of KA and KB was slowed down. Conclusions: KA and KB may be the substrates of CYP2C19. GU plus EK can inhibit the activity of CYP2C19, which resulted in KA and KB metablism slowing down and accumulation. In addition, glycyrrhizic acid and enoxolone can inhibit the metablism of KA and KB. It may be one of the reasons of increased toxicity of GU plus EK.


Shao H.,Shenyang University | Ni Y.,Catholic University of Leuven | Zhang J.,Jiangsu Provincial Academy of Traditional Chinese Medicine | Chen F.,Catholic University of Leuven | And 4 more authors.
PLoS ONE | Year: 2013

Evaluation of vascular disrupting treatment (VDT) is generally based on tumor size and enhancement on conventional magnetic resonance imaging (MRI) which, unfortunately, may be limited in providing satisfactory information. The purpose of the study is to evaluate consecutive changes of 20 rabbit VX2 liver tumors after VDT by dynamic contrast-enhanced MRI (DCE-MRI) and diffusion-weighted imaging (DWI) at a 3.0 T MR unit. Twenty four hours after intravenous injection of Combretastatin A-4-phosphate (CA4P) at 20 mg/kg, DCE-MRI derived Maximum Slope of Increase (MSI) and Positive Enhancement Integral (PEI) decreased sharply due to sudden shutting down of tumor feeding vessels. DWI derived Apparent Diffusion Coefficient (ADC) in tumor periphery decreased because of ischemic cell edema. On day 4, an increase of MSI was probably caused by the recovery of blood supply. A remarkable increase of ADC represented a large scale of necrosis among tumors. On day 8, the blood perfusion further decreased and the extent of necrosis further increased, reflected by lower MSI and PEI values and higher ADC value. On day 12, a second decrease of ADC was noticed because the re-growth of periphery tumor. The experimental data indicate that the therapeutic effects of VDT may be noninvasively monitored with DCE-MRI (reflecting tumor blood perfusion) and DWI (reflecting the changes of histology), which provide powerful measures for assessment of anticancer treatments. © 2013 Shao et al.


Shao H.,Shenyang University | Zhang J.,Jiangsu Provincial Academy of Traditional Chinese Medicine | Sun Z.,Shandong Academy of Sciences | Chen F.,Catholic University of Leuven | And 7 more authors.
Oncotarget | Year: 2015

A viable rim of tumor cells surrounding central necrosis always exists and leads to tumor recurrence after vascular disrupting treatment (VDT). A novel necrosis targeted radiotherapy (NTRT) using iodine-131-labeled hypericin (131I-Hyp) was specifically designed to treat viable tumor rim and improve tumor control after VDT in rabbit models of multifocal VX2 tumors. NTRT was administered 24 hours after VDT. Tumor growth was significantly slowed down by NTRT with a smaller tumor volume and a prolonged tumor doubling time (14.4 vs. 5.7 days), as followed by in vivo magnetic resonance imaging over 12 days. The viable tumor rims were well inhibited in NTRT group compared with single VDT control group, as showed on tumor cross sections at day 12 (1 vs. 3.7 in area). High targetability of 131I-Hyp to tumor necrosis was demonstrated by in vivo SPECT as high uptake in tumor regions lasting over 9 days with 4.26 to 98 times higher radioactivity for necrosis versus the viable tumor and other organs by gamma counting, and with ratios of 7.7-11.7 and 10.5-13.7 for necrosis over peri-tumor tissue by autoradiography and fluorescence microscopy, respectively. In conclusion, NTRT improved the anticancer efficacy of VDT in rabbits with VX2 tumors.


PubMed | Catholic University of Leuven, Shenyang University and Jiangsu Provincial Academy of Traditional Chinese Medicine
Type: Journal Article | Journal: Oncotarget | Year: 2016

Residual tumor resulting in tumor recurrence after various anticancer therapies is an unmet challenge in current clinical oncology. This study aimed to investigate the hypothesis that radioiodinated hypericin (131I-Hyp) may inhibit residual tumor recurrence after microwave ablation (MWA) on rat orthotopic liver allograft sarcoma models.Thirty Sprague-Dawley (SD) rats with hepatic tumors were divided into three groups: Group A received laparotomy MWA and sequential intravenous injection (i.v.) of 131I labelled hypericin (131I-Hyp) in a time interval of 24 h; Group B received only laparotomy MWA; Group C was a blank control. Tumor inhibitory effects were monitored with in vivo magnetic resonance imaging (MRI) and these findings were compared to histopathology data before (baseline, day 0) and 1, 4, and 8 days after MWA. In addition, biodistribution of 131I-Hyp was assessed with in vivo single-photon emission computed tomography-computed tomography (SPECT-CT) imaging, in vitro autoradiography, fluorescent microscopy, and gamma counting.A fast clearance of 131I-Hyp and increasing deposit in necrotic tumors appeared over time, with a significantly higher radioactivity than other organs (0.9169 1.1138 % ID/g, P < 0.01) on day 9. Tumor growth was significantly slowed down in group A compared to group B and C according to MRI images and corresponding tumor doubling time (12.13 1.99, 4.09 0.97, 3.36 0.72 days respectively). The crescent tagerability of 131I-Hyp to necrosis was visualized consistently by autoradiography and fluorescence microscopy.In conclusion, 131I-Hyp induced necrosis targeted radiotherapy improved therapeutic outcomes of MWA on rat orthotopic liver allograft sarcoma models.


Ren A.-N.,Jiangsu Provincial Academy of Traditional Chinese Medicine | Lu Y.,Nanjing University | Zou Y.-F.,Jiangsu University | Shen H.,Jiangsu Provincial Academy of Traditional Chinese Medicine
European Food Research and Technology | Year: 2013

The crude water-soluble polysaccharide (SCP) was extracted from safflower by using hot water, and further fractioned by DEAE-52 anion exchange and Sephadex G-100 gel column chromatography to yield two acidic polysaccharides (SCP2 and SCP3). High-performance gel permeation chromatograph analysis showed that molecular weights (M W) of polysaccharides (SCP2 and SCP3) were 5, 860 and 9,330 Da, respectively. According to Fourier transform infrared spectroscopy, gas chromatography and chemical methods, SCP2 consisted of rhamnose, arabinose, glucose and galactose with a molar ratio of 2.93:11.19:33.68:3.48, while the primary sugar residues were (1 → 2)-linked-rhamnopyranose, (1 → 2)-linked and (1 → 3)-linked-arabinofuranose, (1 → 3)-linked-glucopyranose and (1 → 2)-linked or (1 → 6)-linked-galactopyranose. Likewise, SCP3 was composed of rhamnose, arabinose, xylose, mannose, glucose and galactose in a molar ratio of 4.44:1.46:4.51:5.82:8.23:19.38, while the primary sugar residues were (1 → 2)-linked-rhamnopyranose, (1 → 2)-linked-xylofuranose, (1 → 3)-linked-arabinofuranose, (1 → 2)-linked or (1 → 6)-linked-mannopyranose and glucopyranose, (1 → 2)-linked or (1 → 6)-linked or (1 → 3)-linked-galactopyranose. © 2013 Springer-Verlag Berlin Heidelberg.


Jin P.,Jiangsu Provincial Academy of Traditional Chinese Medicine
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica | Year: 2012

To study the effects of triterpenoid components from Prunella asiatica on phase II detoxifying enzymes and protein expression in vitro and in vivo. Normal human bronchial epithelial (NHBE) cell model was used in vitro, and the mouse model of Kunming (KM) mice was used in vivo. CDNB assay was used to measure the activity of GST. NADPH and DCIP was used to detect the activity of NQO1. DTNB colorimetric assay was used to detect GSH. Western blot was use to detect the protein expression of NQO1. We found that triterpenoid components from P. asiatica could increase the activity of GST, NQO1 and GSH in NHBE cells and KM mice. NQO1 protein expression can also be increased in vitro. The study suggests that triterpenoid components from P. asiatica can prevent the lung cancer by regulating the body phase II detoxification enzyme activity and protein expression.


Peng Y.,Jiangsu Provincial Academy of Traditional Chinese Medicine | Peng Y.,Jiangsu University | Tan X.-B.,Jiangsu Provincial Academy of Traditional Chinese Medicine | Jia X.-B.,Jiangsu Provincial Academy of Traditional Chinese Medicine
Chinese Traditional and Herbal Drugs | Year: 2013

Objective: To study the effects of the flavonoids from Glycyrrhizae Radix (FGR) on the function and expression of P-glycoprotein (P-gp) in Caco-2 cells, and to further explore the detoxification mechanisms of FGR. Methods: Flow cytometry was used to study the effects of FGR on the uptake of Rhodamine 123, which showed the toxic efflux function of P-gp; Western blotting method was used to detect the expression level of P-gp in Caco-2 cells. Results: Compared with the control group, after treated with the total flavonoids from Glycyrrhizae Radix (TFGR) at the different concentration (5, 10, 50, and 100 μg/mL) for 1 h, the uptake of Rhodamine 123 in Caco-2 cells was decreased by 61.1%, 56.3%, 49.2%, and 45.4%; liquiritin, isoliquiritin, liquiritigenin, isoliquiritigenin, liquiritin apioside, and isoliquiritin apioside with the same dose significantly decreased the fluorescence intensity of Rhodamine 123 by 19.3%-37.9%. The expression levels of P-gp in Caco-2 cells were significantly increased (P < 0.01) after the co-incubation of TFGR (10-400 μg/mL) for 72 h, and liquiritin, isoliquiritin, liquiritigenin, and liquiritin apioside (5-400 μmol/L) had the similar functions, so did isoliquiritin and isoliquiritin apioside (10-400 μg/mL, P < 0.01). Conclusion: FGR could strengthen the function, up-regulate the expression of P-gp in Caco-2 cells, promote the efflux of toxic substances, and decrease the absorption of toxic substances, which could be one of the new mechanisms for Glycyrrhizae Radix detoxification.


Wang P.J.,Jiangsu Provincial Academy of Traditional Chinese Medicine
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2011

To study the effect o f Bushen Huoxue Decoction (BHD) on neurobiochemical markers in the hippocampus of female rats with repeated immobilization stress. Sixty female rats were randomly divided into the normal group, the model group, the positive control group (treated with Liuwei Dihuang Pill at the dose of 3.3 g crude drug/kg), and the high, middle, and low BHD treated groups (at the dose of 8, 4, 2 g crude drug/kg), ten in each group. Chronic psychological stress was induced using repeated immobilization stress in rats. Medication was conducted by gastrogavage while modeling once a day for twenty successive days. The hippocampal neurohumoral levels were detected with high-performance liquid chromatography. The expression levels of BDNF and its receptor in the hippocampus were detected by Westem blot. Effect of BHD on neurobiochemical markers in the hippocampus of rats with repeated immobilization stress was observed. The levels of Glu, GABA, and BDNF in the hippocampus of the normal group were 1280.0 +/- 258.3 ng/mg, 588.3 +/- 115.1 ng/mg, and 13.26 +/- 2.57 gray value, respectively. But the hippocampal neurohumoral levels and the expression of BDNF in the model group obviously decreased when compared with the normal group, being 1016.9 +/- 215.9 ng/mg, 485.1 +/- 71.0 ng/mg, and 7.23 +/- 0.61 gray value, respectively. The levels of Glu (ng/mg) in hippocampus of the three BHD treated groups were 1459.1 +/- 413.5, 1894.7 +/- 542.8, and 1373.3 +/- 345.7, respectively. GABA levels (ng/mg) inthe hippocampus were 631.6 +/- 161.4, 899.1 +/- 262.1, and 656.4 +/- 140.8, respectively. BDNF levels (gray value) were 16.57 +/- 1.52, 29.85 +/- 1.37, and 24.44 +/- 3.81, respectively, significantly higher than that of the model group (P<0.05, P<0.01). The level of Glu in the positive control group (1216.5 +/- 193.8 ng/mg) was significantly higher than that of model group (P<0.05). BHD showed significant accommodation on the hippocampal neurohumoral levels and the expression of BDNF in the female rats with repeated immobilization stress.


Yan Y.,China Pharmaceutical University | Chai C.-Z.,China Pharmaceutical University | Wang D.-W.,Jiangsu Provincial Academy of Traditional Chinese Medicine | Yue X.-Y.,China Pharmaceutical University | And 2 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2013

Ge-Gen Decoction (GGD) is a classical formula of traditional Chinese medicine. It is generally used for treating common cold, fever and influenza in China and South East Asia. In this study, a systematic method was established for the qualitative and quantitative analysis of the major constituents in GGD. For qualitative analysis, a method of liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) was developed for identification of multi-constituents. Based on the UV spectra, retention time and MS spectra, sixty compounds in GGD extract were identified or tentatively characterized by comparing with reference substances or literatures. According to the qualitative results, a new quantitative analysis method of GGD was established by HPLC-DAD. Fourteen representative compounds unequivocally identified were chosen as marker components which were derived from five herbs in GGD excluding Zingiberis Rhizoma Recens and Jujubae Fructus. The analytical method was validated through intra- and inter-day precision, repeatability and stability, and the R.S.D. was less than 3.18%, 4.48%, 3.36% and 3.54%, respectively. The LODs and the LOQs for the analytes were less than 1.06 and 3.12μgmL-1, respectively. The overall recoveries ranged from 94.8% to 105.6%, with the R.S.D. ranging from 0.68% to 3.23%. Then the new method was applied to determine twelve batches of GGD commercial products of three dosage forms. The results indicated that the new approach was applicable in the routine analysis and quality control of GGD products. The study might provide a basis for quality control of GGD, and further study of GGD in vivo. © 2013 Elsevier B.V.


PubMed | Shanghai University of Traditional Chinese Medicine and Jiangsu Provincial Academy of Traditional Chinese Medicine
Type: | Journal: Evidence-based complementary and alternative medicine : eCAM | Year: 2015

Proteomics technology, a major component of system biology, has gained comprehensive attention in the area of medical diagnosis, drug development, and mechanism research. On the holistic and systemic theory, proteomics has a convergence with traditional Chinese medicine (TCM). In this review, we discussed the applications of proteomic technologies in diseases-TCM syndrome combination researches. We also introduced the proteomic studies on the in vivo and in vitro effects and underlying mechanisms of TCM treatments using Chinese herbal medicine (CHM), Chinese herbal formula (CHF), and acupuncture. Furthermore, the combined studies of proteomics with other -omics technologies in TCM were also discussed. In summary, this report presents an overview of the recent advances in the application of proteomic technologies in TCM studies and sheds a light on the future global and further research on TCM.

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