Traditional Chinese Medicine Hospital of Jiangsu Province

Nanjing, China

Traditional Chinese Medicine Hospital of Jiangsu Province

Nanjing, China

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Wu H.,Nanjing Southeast University | Lin J.,Hefei University of Technology | Liu P.,Nanjing Southeast University | Huang Z.,Nanjing Southeast University | And 5 more authors.
Biomaterials | Year: 2016

Malignant glioma is one of the most common intracranial tumor with a dismal prognosis. The radiosensitizing effect of silver nanoparticles (AgNPs) on glioma both in vitro and in vivo were demonstrated in the previous studies of our group. However, the underlying mechanism is still unclear. In this present study, the use of antioxidants is employed for the regulating of reactive oxygen species (ROS) in U251 cells treated with various agents, and the results shows that ROS played an essential role in the autophagy inducing and radiosensitization effect of AgNPs. Moreover, the inhibition of protective autophagy with 3-MA is another way to increase ROS, resulting in the increasing of cell death and apoptosis. Taken together, understanding the relationship between the elevated ROS and autophagy and the effect of ROS should be useful to the clinical applications of AgNPs. These findings could potentially be exploited for new therapeutic strategies in glioma radiotherapy. © 2016 Elsevier Ltd


PubMed | Hefei University of Technology, Traditional Chinese Medicine Hospital of Jiangsu Province, University of Electronic Science and Technology of China and Nanjing Southeast University
Type: | Journal: Biomaterials | Year: 2016

Malignant glioma is one of the most common intracranial tumor with a dismal prognosis. The radiosensitizing effect of silver nanoparticles (AgNPs) on glioma both invitro and invivo were demonstrated in the previous studies of our group. However, the underlying mechanism is still unclear. In this present study, the use of antioxidants is employed for the regulating of reactive oxygen species (ROS) in U251cells treated with various agents, and the results shows that ROS played an essential role in the autophagy inducing and radiosensitization effect of AgNPs. Moreover, the inhibition of protective autophagy with 3-MA is another way to increase ROS, resulting in the increasing of cell death and apoptosis. Taken together, understanding the relationship between the elevated ROS and autophagy and the effect of ROS should be useful to the clinical applications of AgNPs. These findings could potentially be exploited for new therapeutic strategies in glioma radiotherapy.


Wu C.,Hebei University | Xie Y.,Hebei University | Gao F.,Traditional Chinese Medicine Hospital of Jiangsu Province | Wang Y.,Hebei University | And 4 more authors.
Gene | Year: 2013

To explore Lgr5 as the possible stem cell marker in human gastric tissue, 259 normal gastric tissues and dissected gastric adenocarcinoma were analyzed by immunohistochemistry, immunofluorescence double staining and qRT-PCR. The results demonstrated that Lgr5 was expressed in the bottom of the normal gastric gland units, and showed a differential expression in gastric adenocarcinoma with varying differentiation. Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands. CD26. +, CD44. +, ALDH1. + and CD133. + cells co-existed with Lgr5. + cells in the stem cell zone of adjacent normal gastric mucosa, and they were detectable in gastric adenocarcinoma but behaved differently. We concluded that Lgr5 may be the adult stem cell marker in human gastric epithelium; Lgr5 and Bmi1 may belong to the same stem cell population; Lgr5, CD26, CD44, ALDH1, and CD133 may be functionally-associated. © 2013 Elsevier B.V.


Li Y.,Fudan University | Cheng L.,Nanjing Medical University | Chen X.,Traditional Chinese Medicine Hospital of Jiangsu Province | Yang B.,Zhejiang University | Wang D.,Fudan University
American Journal of Rhinology and Allergy | Year: 2015

Objective: To further evaluate the efficacy and safety of a pollen blocker cream against dust-mite allergy. Methods: A multicenter, randomized, double-blind, placebo-controlled, crossover trial was conducted in a Chinese population. Patients diagnosed with perennial allergic rhinitis, sensitive to dust-mite allergy including Dermatophagoides farinae and Dermatophagoides pteronyssinus were randomly allocated to receive a pollen blocker cream or placebo, which was applied and spread evenly to the lower internal nose region three times daily for a total of 30 days. The primary outcome measurements for efficacy were total nasal symptom score (TNSS) and individual nasal symptom score (iNSS). Adverse events were also monitored. Results: After application of a pollen blocker, the mean TNSS decreased from 23.1 to 13.8, the decrease of the pollen locker group (9.3) was highly significant compared with the placebo group (5.2; p < 0.001). Similarly, the decreases in iNSSs (rhinorrhea, congestion, sneezing, and itching) between the pollen blocker group and the placebo group were also significant (p < 0.05). In addition, in adults, the pollen blocker led to a remarkably significant decrease in TNSS (9.5) compared with placebo (5.4; p < 0.001); in children, the pollen blocker led to ignificant de ase in TNSS (8.6) compared with placebo (4.8; p < 0.05). No statistical difference was found in the incidence of adverse events between the two groups (p > 0.05), and no severe systematic reactions were observed. Conclusion: Pollen Blocker is a safe and effective alternative to the drugs for treatment of AR, especially for Chinese people allergic to dust-mite allergy. Copyright © 2015, OceanSide Publications, Inc., U.S.A.


Zhang Z.,Nanjing University | Chen G.,Traditional Chinese Medicine Hospital of Jiangsu Province | Zhang D.,Nanjing University
Chinese Physics B | Year: 2014

Exploring new acoustic parameters is essential to develop a noninvasive imaging technique for the surgery of silicone oil tamponades. In this study, the acoustic nonlinearity parameters B/A of varied silicone oil samples (e.g., linear or hyper-branched) are experimentally measured by using a modified thermodynamic method. The results show that: (i) when the concentration of the silicone oil with a molecular weight of 5 × 104 increases from 0.5 g/100 ml to 8 g/100 ml, the corresponding B/A value increases by about 18%, but the acoustic velocity only increases by about 0.1%; (ii) when the molecular weight of the hyper-branched silicone oil is enhanced from 2 × 10 5 to 1 × 106, the B/A value increases by about 22%, while the acoustic velocity is only raised by about 0.2%. This study suggests that the B/A parameter of the silicone oil is more sensitive to the change in its molecular structure than that of the acoustic velocity. Thus, the B/A parameter might be utilized as an effective index for the development and optimization of the noninvasive imaging of the surgery of silicone oil tamponades. © 2014 Chinese Physical Society and IOP Publishing Ltd.


Wu J.,Nanjing University | Tong S.,Nanjing University | Zhan Z.,Nanjing University | Zhang J.,Nanjing University | And 3 more authors.
Biomarkers | Year: 2014

Transforming growth factor-alpha (TGF-α) correlates with deep invasion, advanced stage and poor prognosis in gastric cancer. Genetic variants in the 3′ untranslated region (UTR) of TGF-α gene may influence the stability and post-transcriptional regulation of mRNA and contribute to gastric cancer predisposition. To test this hypothesis, we genotyped five polymorphisms in 3′UTR (rs3771527, rs503314, rs473698, rs3732253 and rs538118) and one in 3′ near region (rs11466306) of the TGF-α gene by polymerase chain reaction-ligation detection reaction methods (PCR-LDR). We found that GA/AA genotype of rs11466306 in the 3′ near gene could increase the risk of overall gastric cancer (adjusted OR=1.499, 95%CI: 1.101-2.041), compared to the wild homozygous GG genotype. Meanwhile, the risk effect was more obvious in the intestinal gastric cancer and gastric noncardia cancer (adjusted OR=1.682, 95%CI: 1.188-2.380; adjusted OR=1.495, 95%CI: 1.072-2.086, respectively), but not for the diffuse type and gastric cardia cancer (p>0.05). CT/TT genotype for rs3732253 in the 3′ UTR was associated with increased risk of intestinal gastric cancer (adjusted OR=1.464, 95%CI: 1.036-2.069), compared to their wild homozygous genotypes. These findings indicate that potentially functional TGF-α gene variant may contribute to the risk of intestinal gastric cancer and/or gastric noncardia cancer and could be used as molecular markers for detecting intestinal gastric cancer and/or gastric noncardia cancer in Chinese Han population. © 2014 Informa UK Ltd. All rights reserved.


Liu P.,Jiangsu University | Jin H.,Jiangsu University | Guo Z.,Nanjing Medical University | Ma J.,Traditional Chinese Medicine Hospital of Jiangsu Province | And 4 more authors.
International Journal of Nanomedicine | Year: 2016

Radiotherapy performs an important function in the treatment of cancer, but resistance of tumor cells to radiation still remains a serious concern. More research on more effective radiosensitizers is urgently needed to overcome such resistance and thereby improve the treatment outcome. The goal of this study was to evaluate and compare the radiosensitizing efficacies of gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) on glioma at clinically relevant megavoltage energies. Both AuNPs and AgNPs potentiated the in vitro and in vivo antiglioma effects of radiation. AgNPs showed more powerful radiosensitizing ability than AuNPs at the same mass and molar concentrations, leading to a higher rate of apoptotic cell death. Furthermore, the combination of AgNPs with radiation significantly increased the levels of autophagy as compared with AuNPs plus radiation. These findings suggest the potential application of AgNPs as a highly effective nano-radiosensitizer for the treatment of glioma. © 2016 Liu et al.


Zhang C.-B.,Traditional Chinese Medicine Hospital of Jiangsu Province | Zhang C.-B.,Nanjing University | Liu Z.,Chongqing Medical University | Guo X.-S.,Nanjing University | Zhang D.,Nanjing University
Chinese Physics B | Year: 2011

Microbubbles promise to enhance the efficiency of ultrasound-mediated drug delivery and gene therapy by taking advantage of artificial cavitation nuclei. The purpose of this study is to examine the ultrasound-induced hemolysis in the application of drug delivery in the presence of microbubbles. To achieve this goal, human red blood cells mixed with microbubbles were exposed to 1-MHz pulsed ultrasound. The hemolysis level was measured by a flow cytometry, and the cavitation dose was detected by a passive cavitation detecting system. The results demonstrate that larger cavitation dose would be generated with the increase of acoustic pressure, which might give rise to the enhancement of hemolysis. Besides the experimental observations, the acoustic pressure dependence of the radial oscillation of microbubble was theoretically estimated. The comparison between the experimental and calculation results indicates that the hemolysis should be highly correlated to the acoustic cavitation. © 2011 Chinese Physical Society and IOP Publishing Ltd.


Chen G.,Nanjing Medical University | Zhu W.,Nanjing Medical University | Shi D.,Traditional Chinese Medicine Hospital of Jiangsu Province | Lv L.,Nanjing Medical University | And 3 more authors.
Oncology Reports | Year: 2010

Radiotherapy is widely used in cancer treatment and biological studies. Multiple mechanisms induced by radiation, especially changes of the expression profile of genes, lead to the disruption of cellular homeostasis. MicroRNAs (miRNAs) are important post-transcriptional gene regulators and play an important role in response to cellular stress. Here we investigated the profiles of miRNA expression following exposure to radiation and the possible role of miRNAs in the modulation of radiosensitivity in the glioblastoma multiform U87MG cell line. MiRNA expression profiles revealed a limited set of miRNAs with altered expression in U87MG cells in response to radiation treatment. MiR-181a, a member of miR-181 family, was one of the down-regulated miRNAs, whose expression was further validated by qRT-PCR. The target mRNAs of radiation-responsive miRNAs were predicted with a target prediction tool. Transiently overexpressed miR-181a significantly sensitized malignant glioma cells to radiation treatment concurrent with the down-regulation of the protein Bcl-2 (B cell lymphoma/lewkmia-2). It indicates that miR-181a may modulate radiosensitivity by targeting Bcl-2 in human malignant glioma cells. These data suggest that radiation can affect miRNA expression, which regulates the cellular response, and miR-181a could be a target for enhancing the effect of radiation treatment on malignant glioma cells.


Yu J.,Nanjing University | Yu J.,Traditional Chinese Medicine Hospital of Jiangsu Province | Chen C.-Y.,Nanjing University | Chen G.,Traditional Chinese Medicine Hospital of Jiangsu Province | And 5 more authors.
Chinese Physics Letters | Year: 2014

A software-based method is proposed to eliminate the flooding interference strips in B-mode images, and to evaluate the cavitation bubbles generated during high intensity focused ultrasound (HIFU) exposures. In vitro tissue phantoms are exposed to 1.12 MHz HIFU pulses with a fixed 100 Hz pulse repetition frequency. HIFU-induced cavitation bubbles are detected as hyperechoic regions in B-mode images. The temporal evolution of cavitation bubbles, generated by HIFU pulses with varying driving amplitude and pulse length, is analyzed by measuring the time-varying area of the hyperechoic region. The results show that: first, it is feasible to monitor HIFU-induced cavitation bubble activity in real-time using B-mode imaging; second, more cavitation bubbles can be generated with higher acoustic energy delivered; third, the hyperechoic region is observed to shrink gradually after ceasing the HIFU pulses, which indicates the dissolution of cavitation bubbles. This work will be helpful for developing an effective tool to realize real-time monitoring and quantitative evaluation of HIFU-induced cavitation bubble activity using a current commercialized B-mode machine. © 2014 Chinese Physical Society and IOP Publishing Ltd.

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