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Chen Y.,Jiangsu Province and oChinese Academy of science | Chen Y.,Nanjing Agricultural University | Zong J.,Jiangsu Province and oChinese Academy of science | Tan Z.,Nanjing Agricultural University | And 5 more authors.
Plant Physiology and Biochemistry | Year: 2015

Though a large number of salt-tolerant genes were identified from Glycophyte in previous study, genes involved in salt-tolerance of halophyte were scarcely studied. In this report, an important halophyte turfgrass, Zoysia matrella, was used for systematic excavation of salt-tolerant genes using full-length cDNA expression library in yeast. Adopting the Gateway-compatible vector system, a high quality entry library was constructed, containing 3×106 clones with an average inserted fragments length of 1.64kb representing a 100% full-length rate. The yeast expression library was screened in a salt-sensitive yeast mutant. The screening yielded dozens of salt-tolerant clones harboring 16 candidate salt-tolerant genes. Under salt-stress condition, these 16 genes exhibited different transcription levels. According to the results, we concluded that the salt-tolerance of Z.matrella might result from known genes involved in ion regulation, osmotic adjustment, as well as unknown pathway associated with protein folding and modification, RNA metabolism, and mitochondrial membrane translocase, etc. In addition, these results shall provide new insight for the future researches with respect to salt-tolerance. © 2015.

Li-ping L.,Nanjing Agricultural University | Xiao-hua L.,Nanjing Agricultural University | Hong-bo S.,CAS Yantai Institute of Coastal Zone Research | Hong-bo S.,Jiangsu Academy of Agricultural Sciences | And 4 more authors.
Ecological Engineering | Year: 2015

In order to improve the coastal saline-alkali soil in north Jiangsu Province, the effects of a newly-developed ameliorant was compared with gypsum and cow manure in field trials in coastal saline soil in Dafeng County, China. Measurements included soil electrical conductivity, pH, soil nutrients, the plant (Salicornia europea L.) growth and ion concentration in stems and roots. The three ameliorants and their combinations improved soil physical and chemical properties and increased the plant height and stem diameter in the order of cow dung>gypsum>new ameliorant. During the experimental period, the soil electrical conductivity of the optimum combination decreased initially and then increased, but was lower than that in the other treatments. Compared with the control,the pH value in the optimum treatment increased by 10.4%, 9.2% and 5.2% in 0-5, 5-20 and 20-40cm soil layers, respectively. The content of soil organic matter in the three soil layers increased by 22%, 28% and 10%, respectively. The total potassium content in different soil layers increased by 23%, 26% and 26%, respectively. In the optimal treatment, the height of S. europea increased by 32% and stem diameter by 20% compared with control. The K+ and Ca2+ concentration in roots and stems of S. europaea was significantly higher in the optimum treatment compared with control. The optimum combination (in t/ha) was: the new ameliorant 45, gypsum 18 and cow dung 300. © 2015 Elsevier B.V.

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