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Han G.,Jiangsu Lixiahe Institute of Agricultural Science | Han G.,Yangzhou University | Li C.,Jiangsu Lixiahe Institute of Agricultural Science | Li C.,Yangzhou University | And 2 more authors.
Journal of Economic Entomology

Plutella xylostella granulovirus (PxGV) and Bacillus thuringiensis (Bt) are both entomopathogens to the diamondback moth, Plutella xylostella (L.). The purpose of the present study was to measure the effect of the combination of PxGV and Bt at sublethal dosages on the development and mortality of diamondback moth in a laboratory setting. Bt and PxGV exhibited synergistic effect on diamondback moth larval mortality and effectively controlled diamondback moth populations with low dose combination treatment. The combination of three parts per million Bt and 1.3 × 103 occlusion bodies per milliliter of PxGV revealed a higher larval mortality compared with the treatment of Bt or PxGV alone. Combination of Bt and PxGV at sublethal concentrations also increased larval duration, reduced oviposition and decreased adult longevity remarkably, resulting in the lowest population trend index among the treatments. The results suggested that the combination of Bt and PxGV at sublethal dosages might provide a valuable way to improve the control efficacy of diamondback moth compared with treatment of Bt or PxGValone. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. Source

Han G.,Jiangsu Lixiahe Institute of Agricultural Science | Xu J.,Jiangsu Lixiahe Institute of Agricultural Science | Liu Q.,Jiangsu Lixiahe Institute of Agricultural Science | Li C.,Jiangsu Lixiahe Institute of Agricultural Science | And 2 more authors.

Cnaphalocrocis medinalis is a major pest of rice in South and South-East Asia. Insecticides are the major means farmers use for management. A naturally occurring baculovirus, C. medinalis granulovirus (CnmeGV), has been isolated from the larvae and this has the potential for use as microbial agent. Here, we described the complete genome sequence of CnmeGV and compared it to other baculovirus genomes. The genome of CnmeGV is 112,060 base pairs in length, has a G+C content of 35.2%. It contains 133 putative open reading frames (ORFs) of at least 150 nucleotides. A hundred and one (101) of these ORFs are homologous to other baculovirus genes including 37 baculovirus core genes. Thirty-two (32) ORFs are unique to CnmeGV with no homologues detected in the GeneBank and 53 tandem repeats (TRs) with sequence length from 25 to 551 nt intersperse throughout the genome of CnmeGV. Six (6) homologous regions (hrs) were identified interspersed throughout the genome. Hr2 contains 11 imperfect palindromes and a high content of AT sequence (about 73%). The unique ORF28 contains a coiled-coil region and a zinc fingerlike domain of 4-50 residues specialized by two C2C2 zinc finger motifs that putatively bound two atoms of zinc. ORF21 encoding a chit-1 protein suggesting a horizontal gene transfer from alphabaculovirus. The putative protein presents two carbohydrate-binding module family 14 (CBM-14) domains rather than other homologues detected from betabaculovirus that only contains one chit-binding region. Gene synteny maps showed the colinearity of sequenced betabaculovirus. Phylogenetic analysis indicated that CnmeGV grouped in the betabaculovirus, with a close relation to AdorGV. The cladogram obtained in this work grouped the 17 complete GV genomes in one monophyletic clade. CnmeGV represents a new crambidae host-isolated virus species from the genus Betabaculovirus and is most closely relative of AdorGV. The analyses and information derived from this study will provide a better understanding of the pathological symptoms caused by this virus and its potential use as a microbial pesticide. © 2016 Han et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source

Xu J.,Jiangsu Lixiahe Institute of Agricultural Science | Li C.-M.,Jiangsu Lixiahe Institute of Agricultural Science | Yang Y.-J.,Zhejiang Academy of Agricultural Sciences | Qi J.-H.,Jiangsu Lixiahe Institute of Agricultural Science | And 4 more authors.
Rice Science

The growth and reproduction of rice leaffolder, Cnaphalocrocis medinalis, fed on an artificial diet were studied. The results showed that the larvae were able to grow and pupate on the artificial diet. The durations of larvae and pupas of C. medinalis on the artificial diet were 28.1 d and 10.1 d, postponed 4.9 d and 1.7 d respectively, compared with those reared with rice leaves. The number of ovipositions was 41.6 per female, 26.2% higher than that fed on rice leaves. Survival rate curve of larvae was a descent function of mortality-age, with no significant differences from the curve of larvae fed on rice leaves. The net reproductive rate (Ro), intrinsic rate of increase (rm) and finite rate of increase (λ) of the population fed on diet were 17.6928, 0.0884 and 1.0924, respectively, and the mean generation time (T) and double time (td) were prolonged 4.9 d and 1.3 d in comparison with the treatment of rice leaves. Population trend index (I) was 3.26, indicating a growing number of the population of C. medinalis fed on artificial diet. © 2012 China National Rice Research Institute. Source

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