Jiangsu Key Laboratory for Biomaterials and Devices

Nanjing, China

Jiangsu Key Laboratory for Biomaterials and Devices

Nanjing, China

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Ding Q.,Jiangsu Key Laboratory for Biomaterials and Devices | Li M.,Jiangsu Key Laboratory for Biomaterials and Devices | Yang F.,Jiangsu Key Laboratory for Biomaterials and Devices | Gu N.,Jiangsu Key Laboratory for Biomaterials and Devices
Materials China | Year: 2016

In recent years, micro-or nano-composite materials have been rapidly developed. Usually, composite materials are composed of two or more different agents in one micro-or nano-unit structure, which realizes the characteristics of multifunction or synergistic functions enhancement. Silver nanoparticle containing micro-or nano-composite materials are one of the most important research branches in the field of biomedical applications. In this review, firstly, the preparation methods of silver/ polymer and silver/ iron oxide composites were summarized, which include emulsion polymerization method, in-situ formation/ reduction method, hole method, ion exchange method, one pot method, seed method, electrostatic interaction method as well as micelle method. Then, their applications have been reviewed. The promising applications on surface enhanced raman scattering, optical imaging, antibacterial, immune detection, electrochemical detection, catalytic degradation and so on have been described in detail. © 2016, The Editorial Board of Materials China. All right reserved.


Ren J.,Jiangsu Key Laboratory for Biomaterials and Devices | Hua X.,Jiangsu Key Laboratory for Biomaterials and Devices | Zhang T.,Jiangsu Key Laboratory for Biomaterials and Devices | Zhang T.,China Institute of Technology | And 4 more authors.
Journal of Applied Polymer Science | Year: 2011

In this work, we described a facile approach to preparing polypropylene (PP) surface functionalized with telechelic poly(lactic-co-glycolic acid) (HO-PLGA-COOH). The PP flakes were first treated with oxygen plasma and then grafted with different molecular weight PLGA (Mw = 50, 100, and 300 K) in dry dichloromethane solution with the addition of phosphorus pentachloride (PCl5). The attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy measurements were used to characterize the composition of the PP surface. The appearance of 1756 cm-1 for carbonyl (C=O) stretching and 1090 cm-1 for C-O-C and the morphologies observations by atomic force microscopy show that PLGA graft covered the surface of PP flake. The IR intensity at 1756 cm-1 increases with the decreasing M w of PLGA, and the molecular weight of PLGA also influence the grafting of PLGA on PP. The contact angle was used to monitor the changes in hydrophilicity of PP flake surface along the treatment procedure. PLGA-grafted PP (PP-g-PLGA) was eventually obtained. © 2011 Wiley Periodicals, Inc.


Yuan C.,Nanjing Southeast University | An Y.,Nanjing Southeast University | Zhang J.,Nanjing Southeast University | Li H.,Nanjing Southeast University | And 4 more authors.
Nanotechnology | Year: 2014

Gene therapy holds great promise for treating cancers, but their clinical applications are being hampered due to uncontrolled gene delivery and expression. To develop a targeted, safe and efficient tumor therapy system, we constructed a tissue-specific suicide gene delivery system by using magnetic nanoparticles (MNPs) as carriers for the combination of gene therapy and hyperthermia on hepatoma. The suicide gene was hepatoma-targeted and hypoxia-enhanced, and the MNPs possessed the ability to elevate temperature to the effective range for tumor hyperthermia as imposed on an alternating magnetic field (AMF). The tumoricidal effects of targeted gene therapy associated with hyperthermia were evaluated in vitro and in vivo. The experiment demonstrated that hyperthermia combined with a targeted gene therapy system proffer an effective tool for tumor therapy with high selectivity and the synergistic effect of hepatoma suppression. © 2014 IOP Publishing Ltd.


Yang F.,Nanjing Southeast University | Yang F.,Jiangsu Key Laboratory for Biomaterials and Devices | Yang F.,State Key Laboratory of Bioelectronics | Chen P.,Nanjing Southeast University | And 21 more authors.
Small | Year: 2010

Microcontainers with embedded superparamagnetic nanoparticles can be changed into bubble microreactors upon treatment with an alternating magnetic field, which acts as a remote trigger for the release of encapsulated material. Exchange of an arginine solution inside and H2O2 solution outside the microcontainers generates NO gas. The microstructure combines ultrasound diagnostic and NO therapeutic functionalities. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.


Yuan C.,Nanjing Southeast University | Wang L.,Nanjing Southeast University | An Y.,Nanjing Southeast University | Wu G.,Nanjing Southeast University | And 2 more authors.
RSC Advances | Year: 2015

Chitosan encapsulated quantum dots (CS-Qdots) exhibit fascinating optical properties and can efficiently deliver genes into cells in a visualized process. By using CS-Qdots as gene carriers, specific hepatocellular carcinoma (HCC) expressed firefly luciferase genes (p[HRE]AFP-luc) were transfected into HCC cells for hepatoma bioluminescence imaging. The results obtained in this study show that nanocarrier CS-Qdots can be excited by the luciferase coded in the genes delivered into the cells. The maximum emission wavelength of the bioluminescence red-shifted from 560 nm to 630 nm. The excitation of CS-Qdots by bioluminescence occurs at the macroscopic scale and is independent of covalent bond. The luciferase gene-loaded CS-Qdots can act as wavelength-tunable self-illuminating probes thus holding potential for improved tumor optical molecular imaging. This journal is © The Royal Society of Chemistry 2015.


Lin M.,Nanjing Southeast University | Lin M.,Taizhou University | Huang J.,Taizhou University | Zhang J.,Nanjing Southeast University | And 10 more authors.
Nanoscale | Year: 2013

Comprehensive therapy based on the integration of hyperthermia, radiation, gene therapy and chemotherapy is a promising area of study in cancer treatment. Using PEI-Mn0.5Zn0.5Fe2O4 nanoparticles (PEI-MZF-NPs) as a gene transfer vector, the authors transfected self-prepared pEgr1-HSV-TK into HepG2 cells and measured the expression of the exogenous gene HSV-TK by RT-PCR. The results showed that HSV-TK was successfully transfected into HepG2 cells and the expression levels of HSV-TK remained stable. Besides, PEI-MZF-NPs were used as magnetic media for thermotherapy to treat hepatoma by magnet-induced heating, combined with radiation-gene therapy. Both in vitro and in vivo results suggest that this combined treatment with gene, radiation and heating has a better therapeutic effect than any of them alone. The apoptotic rate and necrotic rate of the combined treatment group was 51.84% and 15.45%, respectively. In contrast, it was only 20.55% and 6.80% in the radiation-gene group, 7.49% and 3.62% in the radiation-alone group, 15.23% and 7.90% in the heating-alone group, and only 3.52% and 2.16% in the blank control group. The inhibition rate of cell proliferation (88.5%) of the combined treatment group was significantly higher than that of the radiation-gene group (59.5%), radiation-alone group (37.6%) and heating-alone group (60.6%). The tumor volume and mass inhibition rate of the combined treatment group was 94.45% and 93.38%, respectively, significantly higher than 41.28% and 33.58% of the radiation-alone group, 60.76% and 52.18% of the radiation-gene group, 79.91% and 77.40% of the heating-alone group. It is therefore concluded that this combined application of heating, radiation and gene therapy has a good synergistic and complementary effect and PEI-MZF-NPs can act as a novel non-viral gene vector and magnetic induction medium, which offers a viable approach for the treatment of cancer. © 2013 The Royal Society of Chemistry.


Lin M.,Taizhou University | Lin M.,Nanjing Southeast University | Huang J.,Taizhou University | Zhang D.,Nanjing Southeast University | And 7 more authors.
Analytical Cellular Pathology | Year: 2016

An effective strategy has been developed for synthesis of radionuclide immune albumin nanospheres (131I-antiAFPMcAb-GCV-BSA-NPs). In vitro as well as in vivo targeting of 131I-antiAFPMcAb-GCV-BSA-NPs to AFP-positive hepatoma was examined. In cultured HepG2 cells, the uptake and retention rates of 131I-antiAFPMcAb-GCV-BSA-NPs were remarkably higher than those of 131I alone. As well, the uptake rate and retention ratios of 131I-antiAFPMcAb-GCV-BSA-NPs in AFP-positive HepG2 cells were also significantly higher than those in AFP-negative HEK293 cells. Compared to 131I alone, 131I-antiAFPMcAb-GCV-BSA-NPs were much more easily taken in and retained by hepatoma tissue, with a much higher T/NT. Due to good drug-loading, high encapsulation ratio, and highly selective affinity for AFP-positive tumors, the 131I-antiAFPMcAb-GCV-BSA-NPs are promising for further effective radiation-gene therapy of hepatoma. Copyright © 2016 Mei Lin et al.


Lin M.,Taizhou University | Lin M.,Nanjing Southeast University | Zhang D.,Nanjing Southeast University | Zhang D.,Jiangsu Key Laboratory for Biomaterials and Devices | And 6 more authors.
Nanotechnology | Year: 2013

Joint therapy is a promising area of study in cancer treatment. In this paper, we prepared Mn-Zn ferrite (Mn0.5Zn0.5Fe2O4) magnetofluid using PEI as a surfactant, and investigated the anticancer effect of Mn0.5Zn0.5Fe2O4 magnetic fluid hyperthermia (MFH) combined with radiotherapy on hepatocellular carcinoma. Both in vitro and in vivo results suggest that this combined treatment with MFH and radiation has a better therapeutic effect than either of them alone. The apoptotic rate and necrotic rate of the combined treatment group was 38.80 and 25.20%, respectively. In contrast, it was only 7.49 and 3.62% in the radiation-alone group, 15.23 and 7.90% in the MFH-alone group, only 3.52 and 2.16% in the blank control group, and 23.56 and 27.56% in the adriamycin group. The cell proliferation inhibition rate of the combined treatment group (88.5%) was significantly higher than that of the radiation-alone group (37.5%), MFH-alone group (60.6%) and adriamycin group (70.6%). The tumor volume inhibition and mass inhibition rate of the combined treatment group was 87.62 and 88.62%, respectively, obviously higher than the 41.04 and 34.20% of the radiation-alone group, 79.87 and 77.92% of the MFH-alone group and 71.76 and 66.87% of the adriamycin group. It is therefore concluded that this combined application of MFH and radiation can give good synergistic and complementary effects, which offers a viable approach for treatment of cancer. © 2013 IOP Publishing Ltd.


Yang F.,Jiangsu Key Laboratory for Biomaterials and Devices | Yang F.,State Key Laboratory of Bioeletronics | Yang F.,Nanjing Southeast University | Zhang M.,Jiangsu Key Laboratory for Biomaterials and Devices | And 18 more authors.
Small | Year: 2011

Fe3O4 nanoparticles embedded in the shells of encapsulated microbubbles could be used therapeutically as in situ drug-delivery vehicles. Bioeffects on liver tumor cells SMMC-7721 due to the excitation of Fe3O4 nanoparticles attached to microbubbles generated by ultrasound (US) are studied in an in vitro setting. The corresponding release phenomenon of Fe3O4 nanoparticles from the shells of the microbubbles into the cells via sonoporation and related phenomena, including nanoparticle delivery efficiency, cell trafficking, cell apoptosis, cell cycle, and disturbed flow of intracellular calcium ions during this process, are also studied. Experimental observations show that Fe3O4 nanoparticles embedded in the shells of microbubbles can be delivered into the tumor cells; the delivery rate can be controlled by adjusting the acoustic intensity. The living status or behavior of Fe3O4-tagged tumor cells can then be noninvasively tracked by magnetic resonance imaging (MRI). It is further demonstrated that the concentration of intracellular Ca2+ in situ increases as a result of sonoporation. The elevated Ca2+ is found to respond to the disrupted site in the cell membrane generated by sonoporation for the purpose of cell self-resealing. However, the excessive Ca2+ accumulation on the membrane results in disruption of cellular Ca2+ cycling that may be one of the reasons for the death of the cells at the G1 phase. The results also show that the Fe3O 4-nanoparticle-embedded microbubbles have a lower effect on cell bioeffects compared with the non-Fe3O4-nanoparticle- embedded microbubbles under the same US intensity, which is beneficial for the delivery of nanoparticles and simultaneously maintains the cellular viability. Bioeffects due to ultrasound excitation of Fe3O4 nanoparticles attached to microbubbles on liver tumor cells SMMC-7721 are studied in an in vitro setting. The corresponding release phenomenon of Fe 3O4 nanoparticles from the microbubbles' shells into the cells via sonoporation and related phenomena, including nanoparticle delivery efficiency, cell trafficking, cell apoptosis, cell cycle, and disturbed flow of intracellular calcium ions during this process are also studied. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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