Chen M.,Nanjing University of Finance and Economics |
Yuan L.,Nanjing University of Finance and Economics |
Yuan L.,Jiangsu Key Laboratory of Quality Controls and Further Processing of Cereals and Oils |
Gao M.,Nanjing University of Finance and Economics |
And 11 more authors.
Journal of the Chinese Cereals and Oils Association | Year: 2013
We determined and drawn a ternary phase diagram of hydrophilic ionic liquid [Bmim]Cl(1-ethyl-3-methylimidazolium chloride)+K2HPO4+H2O system at 30°C by turbidimetric method, and the obtained solubility curve equations of ionic liquid in the aqueous two-phase system are: w([Bmim]Cl, upper phase)=0.02688+0.91432×exp(w(K2HPO4, upper phase)/0.11339), rupper phase=0.99959; w([Bmim]Cl, lower phase)=-0.04411+28.97445×exp(-w(K2HPO4, lower phase)/0.02826)+0.289 58×exp(-w(K2HPO4, lower phase)/0.67855), rlower phase=0.99124. A new method to extract rapeseed meal protein in aqueous two-phase system formed by [Bmim]Cl and K2HPO4 was studied. The effects of concentration of different ionic liquid, salt and rapeseed protein and solution pH value on the extraction efficiency of rapeseed protein were investigated in the ionic liquid aqueous two-phase system; and through L16(45)orthogonal optimized experiment, the optimum technical conditions of extraction of rapeseed meal protein in ionic liquid aqueous two-phase system were gotten: the concentration of rapeseed K2HPO4 150 mg/mL, [Bmim]Cl 350 mg/mL and rapeseed protein 70.0 mg/L, pH 6.8. The verification experiment results showed that under optimum conditions rapeseed protein extraction rate reached 99.1%, which showed the unique nature of the separation system on extraction of rapeseed protein and laid the experimental foundation for further amplification experiments or industrial scale production.