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Lai X.F.,Jiangsu Key Laboratory of Marine Biotechnology | Lai X.F.,Chinese Academy of Fishery Sciences | Gao H.,Jiangsu Key Laboratory of Marine Biotechnology | Kong J.,Chinese Academy of Fishery Sciences | And 3 more authors.
Aquaculture International | Year: 2011

Glutamine synthetase (GS) is considered to be one of the oldest existing functioning genes in evolution and plays a key role in two major biochemical pathways: in liver GS catalyzes ammonia detoxification, whereas in neural tissues it also functions in recycling of the neurotransmitter glutamate. In this study, a cytosolic glutamine synthetase was cloned and characterized from the Chinese Shrimp Fenneropenaeus chinensis, and named as FcGS. The results indicated that the full-length DNA of 1,756 bp had an open reading frame without intron, which encoded a polypeptide of 366 amino acids with several conserved active site residues. The calculated molecular mass of mature protein was 40. 7 kDa and theoretical pI was 6. 47. A phylogenetic analysis of GS sequences showed that FcGS clustered with the invertebrate group as expected. qRT-PCR analysis indicated that FcGS was expressed in intestines, hepatopancreas, muscles and gills, and its profile was up-regulated post-WSSV challenge in hepatopancreas and gills. Our results suggested that FcGS might participate in the regulation of shrimp immune response toward WSSV invasion. These data would be helpful to better understand the WSSV resistance mechanism of farming shrimps. © 2011 Springer Science+Business Media B.V.

Hu X.-L.,Huaihai Institute of Technology | Hu X.-L.,Jiangsu Key Laboratory of Marine Biotechnology | Shi P.-F.,Huaihai Institute of Technology | Shi P.-F.,Jiangsu Key Laboratory of Marine Biotechnology | And 3 more authors.
Rengong Jingti Xuebao/Journal of Synthetic Crystals | Year: 2013

A new complex [Cu(pht)2(fa)](H2O)2 (Hpht: 5, 5-diphenylhydantoin, i.e. phenytoin; fa: 2-furfurylamine) has been synthesized by the reaction of copper acetate, Hpht and fa. Element analysis, IR and single-crystal X-ray diffraction were used to characterize the chemical composition and crystal structure. The crystal of the Cu(II) complex belongs to triclinic system with space group Pi, a=0.9878(2) nm, b=1.0068(2) nm, c=1.0640(3) nm, α =78.664(3)°, β=75.490(2)°, γ=72.050(3)°, Z=1, Dc=1.368 mg·m-3, R1=0.0986, ωR2=0.2191. In the title complex, Cu(II) ion was coordinated to four N atoms from two Hpht and two fa molecules, forming a square planar geometry. The antimicrobial activity of the complex, Hpht and metal salt against Escherichia Coli, Staphylococcus Aureus and Bacillus. Subtilis were studied by agar diffusion method. The results showed that they are active against the three test bacterial organisms.

Wang H.-B.,Jiangsu Key Laboratory of Marine Biotechnology | Wang H.-B.,Huaihai Institute of Technology | Jiao Y.-L.,Huaihai Institute of Technology | Xu J.-T.,Huaihai Institute of Technology | And 4 more authors.
Fish Pathology | Year: 2011

The gene vah4 encoding Vibrio anguillarum hemolysin was cloned and hetelogously expressed. The purified recombinant VAH4 protein was then injected into Japanese flounder Paralichtys olivaceus as an immunogen. Activities of superoxide dismutase, catalase, alkaline phosphatase and acid phosphatase in liver became higher in the immunized group. However, these improvements vanished in 72 h after the injection. VAH4-specific antibodies were detected at a titer of 1:10,240 by ELISA on the 10th day after the injection. In a separate experiment, fish were immunized with VAH4 twice and challenged with V. anguillarum. The immunization with the VAH4 protected fish from V. anguillarum infection.

Lu Z.-H.,Huaihai Institute of Technology | Lu Z.-H.,Jiangsu Key Laboratory of Marine Biotechnology | Yan B.-L.,Huaihai Institute of Technology | Yan B.-L.,Jiangsu Key Laboratory of Marine Biotechnology | Yang J.-X.,Nanjing Normal University
Zhongguo Huanjing Kexue/China Environmental Science | Year: 2013

Using ecotoxicological approach we studied the effect of bishphenol A (BPA) on the reproductive variables of B. calyciflorus including population growth rate (r), and the mictic female/amicic female (MF/AF), mixis rate (MR), total male number /total female number (M/F), ovigerous females/non-ovigerous females (OF/NOF), fertilization rate (FR), resting egg production (RE), and cyst hatching rates. The results showed that BPA at 0.5mg/L or higher concentration significantly decreased r (0.83~0.86) to control, but BPA had no significant effect on M/F and OF/NOF. BPA from 0.25to 1.0mg/L reduced markedly MF/AF and MR. BPA at 0.125 to 1.0mg/L decreased significantly fertilization rate (3.33%~15.33%) and resting egg production (25.2~39.2 ind/8 mL). BPA at 0.0625to 1.0mg/L also significantly reduced the hatching rates (8.0%~22.0%) of resting eggs. The results showed that different endpoints of reproductive parameters had different sensitivities to BPA. HR of rotifers appeared to be most sensitive to the toxicity by BPA. Thus, since BPA had an adverse effect on the reproduction of the tested rotifer species, its concentrations in natural waterbodies must be monitored to protect the zooplankton.

Wang H.-B.,China University of Mining and Technology | Wang H.-B.,Huaihai Institute of Technology | Chen W.-H.,Huaihai Institute of Technology | Li X.-S.,China University of Mining and Technology | And 2 more authors.
Huanjing Kexue/Environmental Science | Year: 2013

The allelopathy between bacteria and algae is a very complicated physical and ecological phenomenon. A marine bacterium was isolated from the water of a shrimp and crab mix-culturing pond. By 16S rRNA analysis, it was identified as Marinobacter adhaerens HY- 3.Skeletonema costatum, a common dominant species of red-tide microorganism in China, was chosen as the other research object. The allelopathic effect of Marinobacter adhaerens HY-3 on S. costatum was studied. Using the growth mass of Skeletonema costatum and the content of chlorophyll a as the parameters, the effects of HY-3 on the growth and photosynthesis of Skeletonema costatum were studied after co-cultivation and addition of extracellular metabolites of HY-3.The results showed that the growth of S. costatum was inhibited when the concentration of the strain HY-3 was above 104, and the growth mass of the 104, 106 and 108 HY-3 group was 70%, 23% and 22% of the control group respectively on the 10th day, with the content of chlorophyll being 88%, 62% and 60% of the control group, respectively. Therefore, the suppression increased with increasing concentration of HY-3.However, addition of extracellular metabolites of HY-3 had no effect on the growth of S. costatum. Conclusions: M. adhaerens HY-3 had certain allelopathy on S. costatum and affected its growth and photosynthesis. Moreover, interaction between M. adhaerens HY-3 and S. costatum was achieved by their direct contact and the extracellular metabolites did not contain allelopathy factors.

Hu X.-L.,Huaihai Institute of Technology | Hu X.-L.,Jiangsu Key Laboratory of Marine Biotechnology | Shi P.-F.,Huaihai Institute of Technology | Qin J.,Huaihai Institute of Technology | And 3 more authors.
Jiegou Huaxue | Year: 2014

A new binuclear copper complex, [Cu2L2 (pht) 2]2·H2O (Hpht = 5, 5-diphenylhydantoin, i.e. phenytoin; L = 2-diethylaminoethanol), has been synthesized by the solvothermal method, and characterized by elemental analysis, IR and single-crystal X-ray diffraction. The crystal belongs to the monoclinic system, space group C2/c with a = 31.918(3), b = 12.9222(15), c = 19.868(2) Å, β = 98.762(2)°, V = 8098.8(16) Å3, Dc = 1.429 Mg/m3, Z = 4, F(000) = 3640, μ = 1.107 mm-1, R = 0.0560 and wR = 0.1276 (I > 2σ(I)). Each copper(II) in a molecule is coordinated with N and O atoms from ligand L and N atom from ligand Hpht in a tetradentate manner. Moreover, two copper(II) centers are bridged by two O atoms of hydroxyl groups from two L ligands to form a slightly distorted binuclear {Cu2O2} diamond configuration. In addition, the interaction of this complex with calf thymus DNA (CT-DNA) was investigated by UV-vis spectrum and viscometry, and the complex may bind to DNA through intercalation.

Zhang C.,Huaihai Institute of Technology | Zhang C.,Jiangsu Key Laboratory of Marine Biotechnology | Ma D.,Huaihai Institute of Technology | Zhang X.,Huaihai Institute of Technology | And 3 more authors.
Journal of Coordination Chemistry | Year: 2016

Three 3-D metal-organic frameworks (MOFs), [Cd(NDC)(biim-4)]·0.5H2O (1), [Cd2(TDC)2(biim-4)2(H2O)2] (2) and [Zn2(biim-4)2(TDC)2]·2.5H2O (3) (biim-4 = 1,4-bis(1-imidazolyl) butyric alkyl; H2NDC = 1,4-naphthalene dicarboxylic acid; H2TDC = thiophene-2,5-dicarboxylic acid), have been synthesized under hydrothermal conditions and structurally characterized by single X-ray diffraction. The three MOFs have high photocatalytic degradation effects on methyl orange under UV irradiation. Through electronic structure analysis combined with time-dependent density functional theory calculations, catalytic performances of these materials are correlated with the molecular composition and the optoelectronic properties of the samples. © 2016 Taylor and Francis.

Zhang J.,Huaihai Institute of Technology | Duan R.,Huaihai Institute of Technology | Wang Y.,Huaihai Institute of Technology | Yan B.,Jiangsu Key Laboratory of Marine Biotechnology | Xue W.,Huaihai Institute of Technology
Food Hydrocolloids | Year: 2012

The gelatins were extracted from skins of silver carp (. Hypophthalmichthys molitrix) caught in winter and summer, respectively. The physicochemical (molecular weight distribution and melting point) and rheological characteristics (viscosity property), as well as functional properties (emulsifying capacity and stability) of the gelatin from winter silver carp skin were compared with those of the summer equivalent. The results showed the properties of the summer gelatin were superior to those of the winter one, showing higher viscosity, emulsion stability, melting point and lower concentration for gelling. The summer gelatin has slightly denser strands of the gel microstructure which was observed by scanning electron microscopy (SEM). Different properties of gelatins from skin of silver carp may be attributed to the big discrepancy of the environmental temperature in the two seasons. © 2012 Elsevier Ltd.

PubMed | Jiangsu Key Laboratory of Marine Biotechnology and Huaihai Institute of Technology
Type: | Journal: Fish & shellfish immunology | Year: 2016

Edwardsiella tarda has been recognized as an important facultative intracellular pathogen of fish with capability of survival and replication within macrophages. E.tarda-macrophage interactions play a very important role in the defense mechanism of fish against infection. The mechanisms that E.tarda use to infect and persist inside macrophages are not well characterized. To gain insight concerning this process, RAW264.7 cells was used to investigate the interactions between E.tarda and macrophages. Using an invitro model involving RAW264.7 cells, internalization assay demonstrated that MOIs of 10:1 and 100:1 could result in a satisfactory infection rate after a 2h infection period. Consistent with the performance in fish macrophages, E.tarda could survive, replicate and induce iNOS-mediated NO production in RAW264.7 cells. Light and electron microscopy confirmed the internalization and replication of E.tarda in RAW264.7 cells, showing once inside macrophages, numberous bacteria may be destroyed within phagolysosomes and those that successfully subvert phagocyte defenses are capable of extensively replicating within the vacuolar-like compartment in macrophages. In addition, E.tarda-induced apoptosis was observed in RAW264.7 cells in a dose-and time-dependent manner, characterized by increased Annexin V binding and the activation of caspase-3. The results described here indicate that RAW264.7 cells could model the behavior of fish macrophages in response to E.tarda in many ways and may serve as a cell model for study on interactions between E.tarda and macrophages. The successful establishment of E.tarda-invaded RAW264.7 cells model may contribute to providing a basis for more detailed understanding of E.tarda pathogenesis.

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