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Wang H.-B.,China University of Mining and Technology | Wang H.-B.,Huaihai Institute of Technology | Chen W.-H.,Huaihai Institute of Technology | Li X.-S.,China University of Mining and Technology | And 2 more authors.
Huanjing Kexue/Environmental Science | Year: 2013

The allelopathy between bacteria and algae is a very complicated physical and ecological phenomenon. A marine bacterium was isolated from the water of a shrimp and crab mix-culturing pond. By 16S rRNA analysis, it was identified as Marinobacter adhaerens HY- 3.Skeletonema costatum, a common dominant species of red-tide microorganism in China, was chosen as the other research object. The allelopathic effect of Marinobacter adhaerens HY-3 on S. costatum was studied. Using the growth mass of Skeletonema costatum and the content of chlorophyll a as the parameters, the effects of HY-3 on the growth and photosynthesis of Skeletonema costatum were studied after co-cultivation and addition of extracellular metabolites of HY-3.The results showed that the growth of S. costatum was inhibited when the concentration of the strain HY-3 was above 104, and the growth mass of the 104, 106 and 108 HY-3 group was 70%, 23% and 22% of the control group respectively on the 10th day, with the content of chlorophyll being 88%, 62% and 60% of the control group, respectively. Therefore, the suppression increased with increasing concentration of HY-3.However, addition of extracellular metabolites of HY-3 had no effect on the growth of S. costatum. Conclusions: M. adhaerens HY-3 had certain allelopathy on S. costatum and affected its growth and photosynthesis. Moreover, interaction between M. adhaerens HY-3 and S. costatum was achieved by their direct contact and the extracellular metabolites did not contain allelopathy factors.

Zhang C.,Huaihai Institute of Technology | Zhang C.,Jiangsu Key Laboratory of Marine Biotechnology | Ma D.,Huaihai Institute of Technology | Zhang X.,Huaihai Institute of Technology | And 3 more authors.
Journal of Coordination Chemistry | Year: 2016

Three 3-D metal-organic frameworks (MOFs), [Cd(NDC)(biim-4)]·0.5H2O (1), [Cd2(TDC)2(biim-4)2(H2O)2] (2) and [Zn2(biim-4)2(TDC)2]·2.5H2O (3) (biim-4 = 1,4-bis(1-imidazolyl) butyric alkyl; H2NDC = 1,4-naphthalene dicarboxylic acid; H2TDC = thiophene-2,5-dicarboxylic acid), have been synthesized under hydrothermal conditions and structurally characterized by single X-ray diffraction. The three MOFs have high photocatalytic degradation effects on methyl orange under UV irradiation. Through electronic structure analysis combined with time-dependent density functional theory calculations, catalytic performances of these materials are correlated with the molecular composition and the optoelectronic properties of the samples. © 2016 Taylor and Francis.

Zhang J.,Huaihai Institute of Technology | Duan R.,Huaihai Institute of Technology | Wang Y.,Huaihai Institute of Technology | Yan B.,Jiangsu Key Laboratory of Marine Biotechnology | Xue W.,Huaihai Institute of Technology
Food Hydrocolloids | Year: 2012

The gelatins were extracted from skins of silver carp (. Hypophthalmichthys molitrix) caught in winter and summer, respectively. The physicochemical (molecular weight distribution and melting point) and rheological characteristics (viscosity property), as well as functional properties (emulsifying capacity and stability) of the gelatin from winter silver carp skin were compared with those of the summer equivalent. The results showed the properties of the summer gelatin were superior to those of the winter one, showing higher viscosity, emulsion stability, melting point and lower concentration for gelling. The summer gelatin has slightly denser strands of the gel microstructure which was observed by scanning electron microscopy (SEM). Different properties of gelatins from skin of silver carp may be attributed to the big discrepancy of the environmental temperature in the two seasons. © 2012 Elsevier Ltd.

Hu X.-L.,Huaihai Institute of Technology | Hu X.-L.,Jiangsu Key Laboratory of Marine Biotechnology | Shi P.-F.,Huaihai Institute of Technology | Qin J.,Huaihai Institute of Technology | And 3 more authors.
Jiegou Huaxue | Year: 2014

A new binuclear copper complex, [Cu2L2 (pht) 2]2·H2O (Hpht = 5, 5-diphenylhydantoin, i.e. phenytoin; L = 2-diethylaminoethanol), has been synthesized by the solvothermal method, and characterized by elemental analysis, IR and single-crystal X-ray diffraction. The crystal belongs to the monoclinic system, space group C2/c with a = 31.918(3), b = 12.9222(15), c = 19.868(2) Å, β = 98.762(2)°, V = 8098.8(16) Å3, Dc = 1.429 Mg/m3, Z = 4, F(000) = 3640, μ = 1.107 mm-1, R = 0.0560 and wR = 0.1276 (I > 2σ(I)). Each copper(II) in a molecule is coordinated with N and O atoms from ligand L and N atom from ligand Hpht in a tetradentate manner. Moreover, two copper(II) centers are bridged by two O atoms of hydroxyl groups from two L ligands to form a slightly distorted binuclear {Cu2O2} diamond configuration. In addition, the interaction of this complex with calf thymus DNA (CT-DNA) was investigated by UV-vis spectrum and viscometry, and the complex may bind to DNA through intercalation.

Lu Z.-H.,Huaihai Institute of Technology | Lu Z.-H.,Jiangsu Key Laboratory of Marine Biotechnology | Yan B.-L.,Huaihai Institute of Technology | Yan B.-L.,Jiangsu Key Laboratory of Marine Biotechnology | Yang J.-X.,Nanjing Normal University
Zhongguo Huanjing Kexue/China Environmental Science | Year: 2013

Using ecotoxicological approach we studied the effect of bishphenol A (BPA) on the reproductive variables of B. calyciflorus including population growth rate (r), and the mictic female/amicic female (MF/AF), mixis rate (MR), total male number /total female number (M/F), ovigerous females/non-ovigerous females (OF/NOF), fertilization rate (FR), resting egg production (RE), and cyst hatching rates. The results showed that BPA at 0.5mg/L or higher concentration significantly decreased r (0.83~0.86) to control, but BPA had no significant effect on M/F and OF/NOF. BPA from 0.25to 1.0mg/L reduced markedly MF/AF and MR. BPA at 0.125 to 1.0mg/L decreased significantly fertilization rate (3.33%~15.33%) and resting egg production (25.2~39.2 ind/8 mL). BPA at 0.0625to 1.0mg/L also significantly reduced the hatching rates (8.0%~22.0%) of resting eggs. The results showed that different endpoints of reproductive parameters had different sensitivities to BPA. HR of rotifers appeared to be most sensitive to the toxicity by BPA. Thus, since BPA had an adverse effect on the reproduction of the tested rotifer species, its concentrations in natural waterbodies must be monitored to protect the zooplankton.

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