Jiangsu Key Laboratory of Chinese Medicine Processing

Nanjing, China

Jiangsu Key Laboratory of Chinese Medicine Processing

Nanjing, China
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Yu H.-L.,Nanjing University | Yu H.-L.,Jiangsu Key Laboratory of Chinese Medicine Processing | Zhang C.-C.,Nanjing University | Wu H.,Nanjing University | And 4 more authors.
Zhongguo Zhongyao Zazhi | Year: 2017

This study was to investigate the effect of vinegar processing on esculentosides in n-BuOH fraction and the contents of the main toxic components esculentoside B (EsB) and esculentoside C (EsC) in Phytolaccae Radix pieces. n-BuOH fraction of Phytolaccae Radix pieces was processed with vinegar according to the processing method in Chinese Pharmacopoeia. HPLC-MS-MS was adopted to analyze the esculentosides composition changes in n-BuOH fraction before and after vinegar processing. HPLC-ELSD was used to detect EsC and EsB contents in raw and vinegar processed Phytolaccae Radix pieces, and investigate the content changes before and after vinegar processing. Results showed that the esculentosides contents in n-BuOH fraction were significantly decreased except esculentoside A (EsA); there were significant changes in saponins compositions, but no new compounds were generated in n-BuOH fraction after vinegar processing. The contents of EsC and EsB were 0.12% and 0.20% respectively in raw Phytolaccae Radix, and decreased to 0.048% and 0.094% accordingly after vinegar processing. It showed that vinegar processing could significantly change the composition of esculentosides in n-BuOH fraction from Phytolaccae Radix and reduce the contents of toxic components EsC and EsB, indicating the scientificity of vinegar processing for Phytolaccae Radix.


Peng W.-W.,Nanjing University | Peng W.-W.,Jiangsu Key Laboratory of Chinese Medicine Processing | Peng W.-W.,HIGH-TECH | Li W.,Nanjing Municipal Hospital of Traditional Chinese Medicine | And 11 more authors.
Journal of Ethnopharmacology | Year: 2013

Ethnopharmacological relevance Radix Aconiti Lateralis (Fuzi in Chinese, derived from the lateral roots of Aconitum Carmichaeli Debx.) is widely used for the treatment of heart failure, internal cold, arthralgia, diarrhea and edema for thousands of years. It was usually prescribed in combination with Rhizoma Zingiberis (Ganjiang in Chinese, derived from the dry rhizome of Zingiber officinale Rosc.) to decrease toxicity and increase efficacy. Aim of the study In order to investigate the influence of Rhizoma Zingiberis on pharmacokinetics of six Aconitum alkaloids, i.e. aconitine (AC), hypaconitine (HA), mesaconitine (MA), benzoylaconine (BAC), benzoylhypaconine (BHA) and benzoylmesaconine (BMA), in Fuzi-Ganjiang herb couple, the comparative pharmacokinetics of six Aconitum alkaloids after oral administration of Fuzi and Fuzi-Ganjiang aqueous extract was carried out. Materials and methods A sensitive, specific and rapid LC-MS/MS method was developed to determine the six analytes in plasma. Then the rats were randomly divided into two groups and orally administered with Fuzi and Fuzi-Ganjiang aqueous extract. At designated time points after oral administration, the concentrations of the six Aconitum alkaloids in rat plasma were determined, and main pharmacokinetic parameters were investigated using 3P97 (Practical Pharmacokinetics Program Version 1.0). Results Comparing with Fuzi group, both T1/2 and AUC0-t of AC and HA decreased (P<0.05), while T1/2, AUC0-t and Cmax of BAC, BHA increased (P<0.05) in Fuzi-Ganjiang group, which indicated that Ganjiang could promote the elimination of AC and HA and enhance the absorption of BAC, BHA and BMA. Conclusion The differences of pharmacokinetics of Aconitum alkaloids in rat plasma could support those of pharmacologics and toxicity in previous reports between Fuzi and Fuzi-Ganjiang herb couple. The results might be helpful in explaining the mechanism of combination of Fuzi-Ganjiang to decrease toxicity and increase efficacy. © 2013 Elsevier Ireland Ltd. All rights reserved.


Zhu F.-G.,Nanjing University | Shi R.-J.,Cixi City Hospital of Traditional Chinese Medicine | Yu H.-L.,Nanjing University | Yu H.-L.,Jiangsu Key Laboratory of Chinese Medicine Processing | And 4 more authors.
Chinese Traditional and Herbal Drugs | Year: 2012

Objective: To study the irritating inflammation and the influence on contents of inflammatory mediators induced by the toxic raphides from Pinellia ternata. Methods: The mouse capillary permeability experiment, models of peritoneal inflammation in mice and toe swelling in rats were applied to investigating the inflammatory effects of the toxic raphides from P. ternata at different concentration. And the content of inflammatory mediators was detected. Results: The results showed that the suspension of the toxic raphides from P. ternata could enhance the capillary permeability and make the release of prostaglandin E 2(PGE 2), nitric oxide (NO), and malondialdehyde (MDA) in peritoneal exudate increased in a dose-dependent manner. The toxic raphides from P. ternata also could cause toe swelling in rats and significantly increase the concentration of PGE 2 and cyclooxygenase (COX-2) in toes of rats, which showed a typical dose-response relationship in a certain dose range. Conclusion: The toxic raphides from P. ternata could cause severe inflammatory toxicity in vivo.


Peng W.-W.,Nanjing University of Traditional Chinese Medicine | Peng W.-W.,Jiangsu Key Laboratory of Chinese Medicine Processing | Liu S.-S.,Nanjing University of Traditional Chinese Medicine | Liu S.-S.,Jiangsu Key Laboratory of Chinese Medicine Processing | And 9 more authors.
Chinese Pharmaceutical Journal | Year: 2013

OBJECTIVE: To explore the mechanism of compatibility of Radix Aconiti Lateralis Praeparate and Zingiber offiicinale Bosc. by determining the contents of four aconitine alkaloids (hyacinthin, benzoylaconine, benzoylhypaconine and benzoylmesaconine) in decoction made from Radix Aconiti Lateralis Praeparate alone and the co-decoctions made from the two medicinal herbs by HPLC. METHODS: The separation was performed on a Kromasil C18 column (4.6 mm × 250 mm, 5 μm) with a gradient elution of acetonitrile (A) and 0.2% aqueous acetic acid at pH of 6.20 adjusted with triethylamine (B) (0-15 min: 25%-31% A; 15-36 min: 31%-35% A; 36-40 min; 35%-25% A). The column temperature was set at 30°C. The flow rate was 1.0 mL · min-1 and the injection volume was 20 μL. The detection wavelength was set at 240 nm. RESULTS: The four components showed baseline separation. The linearity of this method was good (r≤0.999), and the average recoveries were 96.9%-101.9% with RSD<3%. Compared with the decoction made from Radix Aconiti Lateralis Praeparate alone, the contents of hyacinthin and benzoylmesaconine in the co-decoctions made from Radix Aconiti Lateralis Praeparate and Zingiber offiicinale Bosc. were increased, the contents of benzoylaconine and benzoylhypaconine were decreased, but the sum of the four aconitine alkaloids was increased. CONCLUSION: The compatibility of Radix Aconiti Lateralis Praeparate and Zingiber offiicinale Bosc. can increase the release of aconitine alkaloids. The feasibility of the compatibility of Radix Aconiti Lateralis Praeparate and Zingiber offiicinale Bosc. is elaborated from chemical point of view.


Weng Z.-B.,Nanjing University | Weng Z.-B.,China Pharmaceutical University | Gao Q.-Q.,Nanjing University | Gao Q.-Q.,Jiangsu Key Laboratory of Chinese Medicine Processing | And 10 more authors.
Molecular and Cellular Endocrinology | Year: 2015

Estrogen replacement therapy (ERT) is utilized as a major regime for treatment of postmenopausal osteoporosis at present. However, long-term supplement of estrogen may cause uterine hyperplasia and hypertension leading to a high risk of endometrial cancer and breast cancer. Psoralea corylifolia L. has long been used as tonic and food additives in many countries. Previous studies had found two ingredients in P. corylifolia L.: bavachin and bakuchiol exhibited osteoblastic activity. The present study was designed to investigate the protective effect of bakuchiol and bavachin on ovariectomy-induced bone loss and explore the possible mechanism. In vivo, bakuchiol and bavachin could prevented estrogen deficiency-induced bone loss in ovariectomized rats without uterotrophic activity. In vitro studies suggested that bakuchiol and bavachin induced primary human osteoblast differentiation by up-regulating the Wnt signalling pathway. This study suggests that such a bone-protective role makes them a promising and safe estrogen supplement for the ERT. © 2015 Elsevier Ireland Ltd.


Huang P.,Nanjing University | Huang P.,HIGH-TECH | Huang P.,Jiangsu Key Laboratory of Chinese Medicine Processing | Tan S.,Second Hospital of Nanjing | And 11 more authors.
Journal of Ethnopharmacology | Year: 2014

Ethnopharmacological relevance Ascending and descending theory is a core principle of traditional Chinese medicine (TCM) theories. It plays an essential role in TCM clinical applications. Some TCM medicine has specific properties, which could alter the inclination and direction of their actions. The properties of the ascending and floating process of one herbal medicine are affected by means of herb processing. Wine-processing, which is sautéing with rice wine, is one of the most popular technologies of herb processing. Wine-processing increases the inclination and direction of its actions, thereby producing or strengthening their efficacy in cleaning the upper-energizer heat. Radix scutellariae, the dried roots of Scutellaria baicalensis Georgi, is a well-known TCM used for the treatment of inflammation, pyrexia, jaundice, etc. Recently, wine-processed Radix scutellariae was normally applied in clinical studies for the treatment of upper-energizer syndrome. In order to investigate the effects of wine-processing on ascending and descending of Radix scutellariae, the comparative study of distribution of flavonoids in rat tissues of triple energizers (SanJiao-upper, middle, lower jiao) after oral administration of crude and wine-processed Radix scutellariae aqueous extracts was carried out. Materials and methods The rats were randomly assigned to two groups and orally administered with crude and wine-processed Radix scutellariae aqueous extracts, respectively. At different pre-determined time points after administration, the concentrations of compounds in rat tissue homogenate were determined, and the main tissue pharmacokinetic parameters were investigated. Tissue pharmacokinetic parameters including AUC0-t, t1/2, Tmax and Cmax were calculated using DAS 2.0. An unpaired Student t-test was used to compare the differences in tissue pharmacokinetic parameters between the two groups. All the results were expressed as arithmetic mean±S.D. Results The parameters of Cmax and AUC0-t of some flavonoids in wine-processed Radix scutellariae were remarkably increased (p<0.05, p<0.01, p<0.001) in the rat upper-energizer tissues (lung and heart) compared with those of the crude group. However, in the rat middle- and lower-energizer tissues (spleen, liver and kidney), the C max and AUC0-t of some flavonoids were significantly decreased (p<0.05, p<0.01) compared with the crude group. The main explanation for these differences seems to the effects of wine-processing on ascending and descending theory. Conclusions All of these differences in the distribution of triple energizers after oral administration of crude and wine-processed Radix scutellariae aqueous extracts may lead to the increase of efficacy on the upper-energizer tissues and were in compliance with the ascending and descending theory. Therefore, wine-processing was recommended when Radix scutellariae was used for cleaning the upper-energizer heat and humidity. The obtained knowledge can be used to evaluate the impact of these differences on the efficacy of both the drugs in clinical applications and might be helpful in explaining the effects of wine-processing on ascending and descending theory. © 2014 Elsevier Ireland Ltd. All rights reserved.


Wang X.,Nanjing University | Wang X.,Jiangsu Key Laboratory of Chinese Medicine Processing | He C.,Nanjing University | Chen Y.,Nanjing University | And 3 more authors.
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To determine the plasma protein binding rates of brucine and strychnine in total alkaloids from the seed of Strychnou nux-vomica, and make comparison with the single components at the same concentration. Method: Ultrafiltration was employed to determine the rat the plasma protein binding rate of the alkaloids from the seed of S. nux-vomica. The plasma concentrations were measured by RP-HPLC. Result: The protein binding rates of brucine were (65.60 3.01)%, (68.20 ± 7.80)%, (59.58 ± 3.78)% when the plasma concentrations was 0.520, 1.300, 2.600 mg·L-1, respectively. The protein binding rates of strychnine was (66.17 ± 6.36)%, (67.10 ± 2.52)%, (57.21 ± 0.79)% when the plasma concentrations were 0.936, 2.340, 4.680 mg·L-1 respectively. As to the total alkaloids from the seed of S. nux-vomica, The protein binding rate of brucine was (62.19 ± 2.45)%, (69.55 ± 5.84)%, (61.76 ± 3.68)% when the plasma concentrations were 0.519, 1.288, 2.607 mg·L-1, respectively. And the protein binding rates of strychnine were (54.79 ± 3.55)%, (57.13 ± 4.49)%, (59.31 ± 3.65)% when the plasma concentrations were 0.940, 2.338, 4.674 mg·L-1, respectively. Conclusion: Brucine and strychnine have medium capacity in binding to plasma protein. In comparison with the single component of the same concentration, the protein binding rate of brucine in total alkaloids shows little difference, while there seems to be an obvious decrease for strychnine.


Zhao T.-F.,Nanjing University | Zhang Q.,Nanjing University | Zhang W.,Nanjing University | Wu H.,Nanjing University | And 6 more authors.
Zhongguo Zhongyao Zazhi | Year: 2013

Objective: To study the toxic mechanism of toxic raphides from Pinellia ternata. Method: Mouse peritoneal macrophage in vitro culture model was adopted to study dose-dependent and time-dependent curves of toxic raphides, with TNF-α, IL-1β and IL-6 in supernatant as indexes. Scanning electron microscopy was used to observe the changes in surface morphology of raphides-treated macrophages. Macrophages-neutrophils co-cultured the transport model to study the effect of toxic raphides' stimulation of macrophages on neutrophils migration. Result: Toxic raphides' stimulation of macrophages could cause the increase in the levels of TNF-α, IL-1β and IL-6 released, and showed dose dependence and time dependence. Scanning electron microscopy showed that toxic raphides were swallowed by macrophages, with notable cell membrane creases, increase in the number of pseudopods and decrease in integrity of cell membranes, and could significantly induce migration of neutrophils. Conclusion: The inflammatory process induced by toxic raphides is mainly mediated by macrophages. The toxic mechanism of toxic raphides from P. ternata is that toxic raphides penetrate into tissues to activate resident macrophages, release phagocytic and inflammatory cytokines, and cause migration of neutrophils, which finally results in acute inflammatory response.


Huang P.,Nanjing University | Huang P.,Jiangsu Key Laboratory of Chinese Medicine Processing | Qian X.,Nanjing University | Qian X.,Jiangsu Key Laboratory of Chinese Medicine Processing | And 7 more authors.
Journal of Chromatographic Science | Year: 2015

A high-performance liquid chromatography method coupled with photo array diode detector has been developed and validated for simultaneous quantification of 11 active alkaloids in Rhizoma Coptidis (R.C.). The analysis was performed on a Benetnach-C18 column (250 × 4.6 mm, i.d., 5 μm) using binary gradient elution with 30 mmol/L ammonium bicarbonate water containing 0.7% ammonia solution and 0.1% triethylamine (A) and acetonitrile (B) at a flow rate of 1 mL/min, a column temperature of 35°C and UV detection at 275 nm. All calibration curves showed good linear regression (r > 0.9993) in the range of 6.94-111.03, 0.625-10.10, 6.27-100.14, 31.88-510.50, 16.25-260.70, 19.88-18.20, 3.13-50.70, 0.125-2.14, 16.44-263, 62.5-1,000, 0.125-2.14 μg/mL for magnoflorine, noroxyhydrastinine, jatrorrhizine, columbamine, epiberberine, coptisine, berberubine, worenine, palmatine, berberine, oxyberberine, respectively. It also showed good precision, repeatability and stability for quantification of these 11 alkaloids. The limit of detections and limit of quantitations for the analytes ranged from 0.031 to 0.423 μg/mL and from 0.094 to 1.27 μg/mL, respectively. This method was effective and rapid. The optimized method, which was applied to the determination of alkaloids in crude and wine-processed R.C. samples, was found to be feasible, reliable and suitable for their routine quality control. © The Author [2014]. Published by Oxford University Press. All rights reserved.


PubMed | Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing University and China Pharmaceutical University
Type: | Journal: Molecular and cellular endocrinology | Year: 2015

Estrogen replacement therapy (ERT) is utilized as a major regime for treatment of postmenopausal osteoporosis at present. However, long-term supplement of estrogen may cause uterine hyperplasia and hypertension leading to a high risk of endometrial cancer and breast cancer. Psoralea corylifolia L. has long been used as tonic and food additives in many countries. Previous studies had found two ingredients in P. corylifolia L.: bavachin and bakuchiol exhibited osteoblastic activity. The present study was designed to investigate the protective effect of bakuchiol and bavachin on ovariectomy-induced bone loss and explore the possible mechanism. In vivo, bakuchiol and bavachin could prevented estrogen deficiency-induced bone loss in ovariectomized rats without uterotrophic activity. In vitro studies suggested that bakuchiol and bavachin induced primary human osteoblast differentiation by up-regulating the Wnt signalling pathway. This study suggests that such a bone-protective role makes them a promising and safe estrogen supplement for the ERT.

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