Jiangsu Key Laboratory of Chinese Medicine Processing

Nanjing, China

Jiangsu Key Laboratory of Chinese Medicine Processing

Nanjing, China

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Yu H.-L.,Nanjing University | Yu H.-L.,Jiangsu Key Laboratory of Chinese Medicine Processing | Zhang C.-C.,Nanjing University | Wu H.,Nanjing University | And 4 more authors.
Zhongguo Zhongyao Zazhi | Year: 2017

This study was to investigate the effect of vinegar processing on esculentosides in n-BuOH fraction and the contents of the main toxic components esculentoside B (EsB) and esculentoside C (EsC) in Phytolaccae Radix pieces. n-BuOH fraction of Phytolaccae Radix pieces was processed with vinegar according to the processing method in Chinese Pharmacopoeia. HPLC-MS-MS was adopted to analyze the esculentosides composition changes in n-BuOH fraction before and after vinegar processing. HPLC-ELSD was used to detect EsC and EsB contents in raw and vinegar processed Phytolaccae Radix pieces, and investigate the content changes before and after vinegar processing. Results showed that the esculentosides contents in n-BuOH fraction were significantly decreased except esculentoside A (EsA); there were significant changes in saponins compositions, but no new compounds were generated in n-BuOH fraction after vinegar processing. The contents of EsC and EsB were 0.12% and 0.20% respectively in raw Phytolaccae Radix, and decreased to 0.048% and 0.094% accordingly after vinegar processing. It showed that vinegar processing could significantly change the composition of esculentosides in n-BuOH fraction from Phytolaccae Radix and reduce the contents of toxic components EsC and EsB, indicating the scientificity of vinegar processing for Phytolaccae Radix.


Zhu F.-G.,Nanjing University | Shi R.-J.,Cixi City Hospital of Traditional Chinese Medicine | Yu H.-L.,Nanjing University | Yu H.-L.,Jiangsu Key Laboratory of Chinese Medicine Processing | And 4 more authors.
Chinese Traditional and Herbal Drugs | Year: 2012

Objective: To study the irritating inflammation and the influence on contents of inflammatory mediators induced by the toxic raphides from Pinellia ternata. Methods: The mouse capillary permeability experiment, models of peritoneal inflammation in mice and toe swelling in rats were applied to investigating the inflammatory effects of the toxic raphides from P. ternata at different concentration. And the content of inflammatory mediators was detected. Results: The results showed that the suspension of the toxic raphides from P. ternata could enhance the capillary permeability and make the release of prostaglandin E 2(PGE 2), nitric oxide (NO), and malondialdehyde (MDA) in peritoneal exudate increased in a dose-dependent manner. The toxic raphides from P. ternata also could cause toe swelling in rats and significantly increase the concentration of PGE 2 and cyclooxygenase (COX-2) in toes of rats, which showed a typical dose-response relationship in a certain dose range. Conclusion: The toxic raphides from P. ternata could cause severe inflammatory toxicity in vivo.


Gong L.,Nanjing University | Wu H.,Nanjing University | Wu H.,Jiangsu Key Laboratory of Chinese Medicine Processing | Yu H.-L.,Nanjing University | And 3 more authors.
Zhongguo Zhongyao Zazhi | Year: 2013

Objective: To extract and separate toxic components from Phytolaccae Radix, and to comare the changes in toxicity of Phytolaccae Radix before and after being processed with vinegar. Method: The mucous membrane irritation response, mouse peritoneal inflammation model and in vitro macrophages release NO model were applied to compared the changes in inflammatory toxicity of toxic components from Phytolaccae Radix before and after being processed with vinegar. Result: Toxic components of Phytolacca Radix had significant inflammatory toxicity, which could cause conjunctival edema in rabbits, and increase of PGE2 and macrophages release NO content in peritoneal exudate in mice. After being processed with vinegar, they showed reduced irritation, which resulted in decrease of PGE2 and macrophages release NO content in peritoneal exudate in mice. Conclusion: After being processed with vinegar, the toxicity of toxic components from Phytolacca Radix decreased obviously.


Yu H.-L.,Nanjing University | Yu H.-L.,Jiangsu Key Laboratory of Chinese Medicine Processing | Zhang Q.,Nanjing University | Jin Y.-P.,Nanjing University | And 5 more authors.
Zhongguo Zhongyao Zazhi | Year: 2016

In order to compare the effect of sulfur fumigation processing and direct hot air heating technology on puerarin contents and efficacy of Puerariae Thornsonii Radix, the fresh mots of Pueraria thomsonii were cut into small pieces and prepared into direct sunshine drying samples, direct hot air drying samples, and sulfur fumigation-hot air drying samples. Moisture contents of the samples were then determined. The puerarin contents of different samples were compared by HPLC method Moreover, the models of drunkenness mice were established, and then with si peroxide dismutase (SOD) content as the index, aqueous decoction extracts of Puerariae Thomsonii Radix samples with sulfur furnigat on processing and non-sulfur fumigation processing methods were administrated by ig; the effects of sulfur fumigation on contents of SOI in mice liver and serum were determined, and the sulfur fumigation samples and non-sulfur fumigation samples were investigated for moth and mildew under different packaging and storage conditions. Results showed that the sulfur fumigation samples significantly changed the puerarin content from Puerariae Thomsonii Radix. The content of puerarin was de-creased gradually when increasing the times of sulfur fumigation and amount of sulfur. SOD content in drunken mice liver and serum was significantly decreased when increasing the times of sulfur fumigation, showing significant difference with both direct sunshine drying group and direct hot air drying group. Moth and mildew were not found in the sulfur fumigation samples and direct hot air drying samples whose moisture contents were lower than the limit in Pharmacopoeia. Research showed that sulfur fumigation (ran significantly reduce the content of main active ingredients and reduce the efficacy of Puerariae Thomsonii Radix, indicating that the quality of Puer- Ariae Thomsonii Radix was significantly decreased after sulfur fumigation. However, the contents of the main active ingredients, efficacy and storage results of the direct hot air dr ing samples were similar to those in direct sunshine drying samples, so the hot air drying process was a nice drying technology which could be promoted for use.


Weng Z.-B.,Nanjing University | Weng Z.-B.,China Pharmaceutical University | Gao Q.-Q.,Nanjing University | Gao Q.-Q.,Jiangsu Key Laboratory of Chinese Medicine Processing | And 10 more authors.
Molecular and Cellular Endocrinology | Year: 2015

Estrogen replacement therapy (ERT) is utilized as a major regime for treatment of postmenopausal osteoporosis at present. However, long-term supplement of estrogen may cause uterine hyperplasia and hypertension leading to a high risk of endometrial cancer and breast cancer. Psoralea corylifolia L. has long been used as tonic and food additives in many countries. Previous studies had found two ingredients in P. corylifolia L.: bavachin and bakuchiol exhibited osteoblastic activity. The present study was designed to investigate the protective effect of bakuchiol and bavachin on ovariectomy-induced bone loss and explore the possible mechanism. In vivo, bakuchiol and bavachin could prevented estrogen deficiency-induced bone loss in ovariectomized rats without uterotrophic activity. In vitro studies suggested that bakuchiol and bavachin induced primary human osteoblast differentiation by up-regulating the Wnt signalling pathway. This study suggests that such a bone-protective role makes them a promising and safe estrogen supplement for the ERT. © 2015 Elsevier Ireland Ltd.


Huang P.,Nanjing University | Huang P.,HIGH-TECH | Huang P.,Jiangsu Key Laboratory of Chinese Medicine Processing | Tan S.,Second Hospital of Nanjing | And 11 more authors.
Journal of Ethnopharmacology | Year: 2014

Ethnopharmacological relevance Ascending and descending theory is a core principle of traditional Chinese medicine (TCM) theories. It plays an essential role in TCM clinical applications. Some TCM medicine has specific properties, which could alter the inclination and direction of their actions. The properties of the ascending and floating process of one herbal medicine are affected by means of herb processing. Wine-processing, which is sautéing with rice wine, is one of the most popular technologies of herb processing. Wine-processing increases the inclination and direction of its actions, thereby producing or strengthening their efficacy in cleaning the upper-energizer heat. Radix scutellariae, the dried roots of Scutellaria baicalensis Georgi, is a well-known TCM used for the treatment of inflammation, pyrexia, jaundice, etc. Recently, wine-processed Radix scutellariae was normally applied in clinical studies for the treatment of upper-energizer syndrome. In order to investigate the effects of wine-processing on ascending and descending of Radix scutellariae, the comparative study of distribution of flavonoids in rat tissues of triple energizers (SanJiao-upper, middle, lower jiao) after oral administration of crude and wine-processed Radix scutellariae aqueous extracts was carried out. Materials and methods The rats were randomly assigned to two groups and orally administered with crude and wine-processed Radix scutellariae aqueous extracts, respectively. At different pre-determined time points after administration, the concentrations of compounds in rat tissue homogenate were determined, and the main tissue pharmacokinetic parameters were investigated. Tissue pharmacokinetic parameters including AUC0-t, t1/2, Tmax and Cmax were calculated using DAS 2.0. An unpaired Student t-test was used to compare the differences in tissue pharmacokinetic parameters between the two groups. All the results were expressed as arithmetic mean±S.D. Results The parameters of Cmax and AUC0-t of some flavonoids in wine-processed Radix scutellariae were remarkably increased (p<0.05, p<0.01, p<0.001) in the rat upper-energizer tissues (lung and heart) compared with those of the crude group. However, in the rat middle- and lower-energizer tissues (spleen, liver and kidney), the C max and AUC0-t of some flavonoids were significantly decreased (p<0.05, p<0.01) compared with the crude group. The main explanation for these differences seems to the effects of wine-processing on ascending and descending theory. Conclusions All of these differences in the distribution of triple energizers after oral administration of crude and wine-processed Radix scutellariae aqueous extracts may lead to the increase of efficacy on the upper-energizer tissues and were in compliance with the ascending and descending theory. Therefore, wine-processing was recommended when Radix scutellariae was used for cleaning the upper-energizer heat and humidity. The obtained knowledge can be used to evaluate the impact of these differences on the efficacy of both the drugs in clinical applications and might be helpful in explaining the effects of wine-processing on ascending and descending theory. © 2014 Elsevier Ireland Ltd. All rights reserved.


Wang X.,Nanjing University | Wang X.,Jiangsu Key Laboratory of Chinese Medicine Processing | He C.,Nanjing University | Chen Y.,Nanjing University | And 3 more authors.
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To determine the plasma protein binding rates of brucine and strychnine in total alkaloids from the seed of Strychnou nux-vomica, and make comparison with the single components at the same concentration. Method: Ultrafiltration was employed to determine the rat the plasma protein binding rate of the alkaloids from the seed of S. nux-vomica. The plasma concentrations were measured by RP-HPLC. Result: The protein binding rates of brucine were (65.60 3.01)%, (68.20 ± 7.80)%, (59.58 ± 3.78)% when the plasma concentrations was 0.520, 1.300, 2.600 mg·L-1, respectively. The protein binding rates of strychnine was (66.17 ± 6.36)%, (67.10 ± 2.52)%, (57.21 ± 0.79)% when the plasma concentrations were 0.936, 2.340, 4.680 mg·L-1 respectively. As to the total alkaloids from the seed of S. nux-vomica, The protein binding rate of brucine was (62.19 ± 2.45)%, (69.55 ± 5.84)%, (61.76 ± 3.68)% when the plasma concentrations were 0.519, 1.288, 2.607 mg·L-1, respectively. And the protein binding rates of strychnine were (54.79 ± 3.55)%, (57.13 ± 4.49)%, (59.31 ± 3.65)% when the plasma concentrations were 0.940, 2.338, 4.674 mg·L-1, respectively. Conclusion: Brucine and strychnine have medium capacity in binding to plasma protein. In comparison with the single component of the same concentration, the protein binding rate of brucine in total alkaloids shows little difference, while there seems to be an obvious decrease for strychnine.


Zhao T.-F.,Nanjing University | Zhang Q.,Nanjing University | Zhang W.,Nanjing University | Wu H.,Nanjing University | And 6 more authors.
Zhongguo Zhongyao Zazhi | Year: 2013

Objective: To study the toxic mechanism of toxic raphides from Pinellia ternata. Method: Mouse peritoneal macrophage in vitro culture model was adopted to study dose-dependent and time-dependent curves of toxic raphides, with TNF-α, IL-1β and IL-6 in supernatant as indexes. Scanning electron microscopy was used to observe the changes in surface morphology of raphides-treated macrophages. Macrophages-neutrophils co-cultured the transport model to study the effect of toxic raphides' stimulation of macrophages on neutrophils migration. Result: Toxic raphides' stimulation of macrophages could cause the increase in the levels of TNF-α, IL-1β and IL-6 released, and showed dose dependence and time dependence. Scanning electron microscopy showed that toxic raphides were swallowed by macrophages, with notable cell membrane creases, increase in the number of pseudopods and decrease in integrity of cell membranes, and could significantly induce migration of neutrophils. Conclusion: The inflammatory process induced by toxic raphides is mainly mediated by macrophages. The toxic mechanism of toxic raphides from P. ternata is that toxic raphides penetrate into tissues to activate resident macrophages, release phagocytic and inflammatory cytokines, and cause migration of neutrophils, which finally results in acute inflammatory response.


Huang P.,Nanjing University | Huang P.,Jiangsu Key Laboratory of Chinese Medicine Processing | Qian X.,Nanjing University | Qian X.,Jiangsu Key Laboratory of Chinese Medicine Processing | And 7 more authors.
Journal of Chromatographic Science | Year: 2015

A high-performance liquid chromatography method coupled with photo array diode detector has been developed and validated for simultaneous quantification of 11 active alkaloids in Rhizoma Coptidis (R.C.). The analysis was performed on a Benetnach-C18 column (250 × 4.6 mm, i.d., 5 μm) using binary gradient elution with 30 mmol/L ammonium bicarbonate water containing 0.7% ammonia solution and 0.1% triethylamine (A) and acetonitrile (B) at a flow rate of 1 mL/min, a column temperature of 35°C and UV detection at 275 nm. All calibration curves showed good linear regression (r > 0.9993) in the range of 6.94-111.03, 0.625-10.10, 6.27-100.14, 31.88-510.50, 16.25-260.70, 19.88-18.20, 3.13-50.70, 0.125-2.14, 16.44-263, 62.5-1,000, 0.125-2.14 μg/mL for magnoflorine, noroxyhydrastinine, jatrorrhizine, columbamine, epiberberine, coptisine, berberubine, worenine, palmatine, berberine, oxyberberine, respectively. It also showed good precision, repeatability and stability for quantification of these 11 alkaloids. The limit of detections and limit of quantitations for the analytes ranged from 0.031 to 0.423 μg/mL and from 0.094 to 1.27 μg/mL, respectively. This method was effective and rapid. The optimized method, which was applied to the determination of alkaloids in crude and wine-processed R.C. samples, was found to be feasible, reliable and suitable for their routine quality control. © The Author [2014]. Published by Oxford University Press. All rights reserved.


PubMed | Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing University and China Pharmaceutical University
Type: | Journal: Molecular and cellular endocrinology | Year: 2015

Estrogen replacement therapy (ERT) is utilized as a major regime for treatment of postmenopausal osteoporosis at present. However, long-term supplement of estrogen may cause uterine hyperplasia and hypertension leading to a high risk of endometrial cancer and breast cancer. Psoralea corylifolia L. has long been used as tonic and food additives in many countries. Previous studies had found two ingredients in P. corylifolia L.: bavachin and bakuchiol exhibited osteoblastic activity. The present study was designed to investigate the protective effect of bakuchiol and bavachin on ovariectomy-induced bone loss and explore the possible mechanism. In vivo, bakuchiol and bavachin could prevented estrogen deficiency-induced bone loss in ovariectomized rats without uterotrophic activity. In vitro studies suggested that bakuchiol and bavachin induced primary human osteoblast differentiation by up-regulating the Wnt signalling pathway. This study suggests that such a bone-protective role makes them a promising and safe estrogen supplement for the ERT.

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