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Liu-Liu M.,Jiangsu Key Laboratory of Animal Genetics and Breeding and Molecular Design | Liu-Liu M.,Yangzhou University | Xiao-Ying W.,Jiangsu Key Laboratory of Animal Genetics and Breeding and Molecular Design | Xiao-Ying W.,Yangzhou University | And 10 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

To clone the IL-10 promoter of rabbit and analyse its sequence, this study amplified the 5 flanking region of rabbit IL-10, compared the genomes and used bioinformatics software to make a forecast. Then, the researchers comfirmed partial promoter sequences and analysed some transcription factors of the IL-10 gene promoter. The study showed that rabbit and human IL-10 gene promoter had some similarities which all cotained GAGA, CAAT and TATA. The transcriptional start site started from -80 bp of translation initiation codon (ATG) upstream region. The promoter also had some transcription factors such as AP-1, Myogenin, c-Ets-1, C/EBP alpha and so on. Results of this study predicted partial sequences, transcnptional start site and some transcriptional regulating factors binding sites of IL-10 gene promoter which would lay the foundation of lucubrating the expression and regulation of rabbit IL-10 gene. © Medwell Journals, 2012.

Wan X.,Yangzhou University | Wan X.,Jiangsu Key Laboratory of Animal Genetics and Breeding and Molecular Design | Mao L.,Yangzhou University | Mao L.,Jiangsu Key Laboratory of Animal Genetics and Breeding and Molecular Design | And 8 more authors.
Journal of Veterinary Medical Science | Year: 2014

Interleukin-10 (IL-10) has been recently identified as a multifunctional cytokine, because of its close link with immunoregulation and anti-inflammatory responses. This study investigated the association of IL-10 genetic polymorphisms with the immune traits of New Zealand white rabbits (N-W), Fujian yellow rabbits (F-Y) and their reciprocal crosses (N-Y and Y-N, respectively). SNPs on five exons of the IL-10 gene were genotyped in 204 healthy rabbits via PCR-SSCP and DNA sequencing. Two SNPs (A1435G and G1519A, both were synonymous mutations) and six genotypes (AA, BB, CC, AB, AC and BC) were found on exon 3 and one SNP (T base insertion between loci 2532 and 2533, which caused a frameshift mutation), and three genotypes (OO, TT and TO) were present on exon 4. Allele A was the most frequent allele on exon 3 (from 0.548 to 0.771), whereas O was the most frequent on exon 4 (from 0.808 to 0.968). These four populations were all in Hardy-Weinberg equilibrium on both exon3 and exon4. Association analysis between polymorphisms and immune parameters showed that SNPs on exon 3 were significantly associated with immune traits, while SNP on exon 4 may not significantly affect immune traits, but the mechanism is yet to be further studied. © 2014 The Japanese Society of Veterinary Science.

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