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He R.,Jiangsu University | He R.,Jiangsu Key Laboratory of Agricultural Product Physical Processing | Ma H.,Jiangsu University | Ma H.,Jiangsu Key Laboratory of Agricultural Product Physical Processing | Wang H.,Jiangsu University
Journal of Electromagnetic Waves and Applications | Year: 2014

This study investigated the inactivation effect of high-intensity pulsed electromagnetic field (PEF) on Escherichia coli ATCC 8099 and explored the primary mechanism of PEF on the microbe. After PEF treatment, the survival rate (SR) of E. coli 8099 remarkably decreased. When treated by 2.5 T intensity PEF at 30 °C for 20 pulses, 18.3% SR of E. coli cultures was observed. The experimental results also indicated that E. coli 8099 in the log phase was more sensitive to PEF treatment and a change in the PEF treatment temperature, intensity or pulse number significantly affected the inactivation of E. coli 8099. The inactivation effect of PEF treatment was found to be related to the change of the intracellular Ca2+ concentration ([Ca 2+]i) of E. coli 8099; i.e. an increase in [Ca 2+]i was a possible factor in the inactivation of E. coli cells by PEF treatment. © 2014 Taylor & Francis. Source


He R.,Jiangsu University | He R.,Jiangsu Key Laboratory of Agricultural Product Physical Processing | Liu L.,Jiangsu University | Liu L.,Jiangsu Key Laboratory of Agricultural Product Physical Processing | And 6 more authors.
Journal of Chinese Institute of Food Science and Technology | Year: 2013

Rapeseed meal was used to produce rapeseed antioxidant peptides with Bacillus subtilis liquid-state fermentation. Five factors, material/liquid ratio, initial pH, fermentation temperature, fermentation time and inoculation amounts were tested respectively with the two fermentation parameters as indexes namely rapeseed peptides yield and DPPH radical scavenging. Based on these single factor tests, fermentation temperature, fermentation time and inoculation amounts were analyzed by response surface analysis. The result showed that the Bacillus subtilis had higher rapeseed peptides yield. Under material/liquid ratio of 1:15, initial pH 7.0 and rotary speed was 180 r/min, the optimal conditions are fermentation temperature 37°C, fermentation time 33 h and inoculation amounts 2 mL/100 mL. Under the above conditions, the rapeseed peptides rate reaches 20.18% and the half inhibition concentration for scavenging DPPH radical was 0.1605 mg/mL. Source

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