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Yao W.,Nanjing University | Cao L.,Nanjing University | Shan M.,Nanjing University | Zhang L.,Nanjing University | And 2 more authors.
Zhongguo Zhongyao Zazhi | Year: 2010

Objective: The spikes of Schizonepeta tenuifolia from different habits were predicted by UV-Vis spectrum. Method: The dimensions of spectrum data obtained from ten habits were reduced by principal component analysis, and the first six new variables with 99.82% of cumulative reliability were put into the backpropagation neural network for model building. Result: The recognition rate of backpropagation neural network coupled with principal component analysis (PCA-BPNN) was 100%, and its mean square error was 0.001 0. Conclusion: PCA-BPNN can be used for the classifying of spikes of S. tenuifolia from different producing area, and this method is simple and fast.

Zhang W.,Nanjing University | Lu Y.,Nanjing University | Lu Y.,Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research
Zhongguo Zhongyao Zazhi | Year: 2010

Salvia miltiorrhiza contains a variety of anti-tumor active ingredient, such as the water-soluble components (salvianolic acid A, salvianolic acid B, salvinal) and liposoluble constituents (tanshinone I, tanshinone IIA, dihydrotanshinone I, miltirone, cryptotanshinone, ailantholide, neo-tanshinlactone, and nitrogen-containing compounds). These anti-tumor active components play important roles in the different stages of tumor evolution, progression and metastasis. The discovery of new anti-tumor active ingredients must benefits the application of Salvia miltiorrhiza for clinical tumor treatment.

Geng T.,Nanjing University | Zhu H.,Nanjing University | Zhang L.,Nanjing University | Shan M.-Q.,Nanjing University | And 2 more authors.
Chinese Traditional and Herbal Drugs | Year: 2011

Objective: To study the gastrointestinal absorption kinetics of schizonepetin in rats. Methods: The drug concentration by in situ perfusion in rats was determined by HPLC and the volume of perfusion fluid was adjusted by Phenol red labeling. Results: The hourly absorption percentages of three different drug concentrations (1.84, 3.68, and 7.36 μg/mL) in stomachs were (19.47 ± 0.69) %, (21.66 ± 1.92) %, and (26.51 ± 1.25) %, respectively. The absorption rate constants (Ka) of three different concentrations in intestine were (0.203 ± 0.007), (0.159 ± 0.011), and (0.134 ± 0.012) h-1, respectively, which had significant differences among them (P<0.05). Conclusion: Schizonepetin might be absorbed in stomachs via passive transport mechanism and in intestine via active transport mechanism.

Liu D.-L.,Nanjing University | Geng T.,Nanjing University | Sun Y.,Nanjing University | Yao W.-F.,Nanjing University | And 3 more authors.
Chinese Journal of New Drugs | Year: 2012

Objective: To study the plasma protein binding rate of schizonepetin in rats. Methods: Equilibrium dialysis was used to determine the in vitro binding rate of schizonepetin to rat plasma. A HPLC method for the determination of schizonepetin was established and then the plasma protein binding rate in rats was calculated. Results: Schizonepetin and internal standard were separated completely with no interference from other ingredients. The calibration curve of schizonepetin was in good linearity over the range of 0.05~50.3 μg·mL -1. The intra-day and inter-day precision and recovery of schizonepetin met the requirements of methodology. The plasma protein binding rate of schizonepetin at low, middle and high concentrations (0.08, 0.63, 6.30 μg·mL -1) were (62.23±1.25)%, (62.71±0.04)% and (63.99±0.79)%, respectively. Conclusion: The method is simple, rapid, accurate, and sensitive, and it can meet the requirement for analysis method of biological samples. Schizonepetin has medium plasma protein binding rate in rats, with no significant relation to drug concentrations in dialyzate.

Yao W.,Nanjing University | Yao W.,Empirical | He M.,Nanjing University | He M.,Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research | And 7 more authors.
Chromatographia | Year: 2013

A comprehensive metabolomic strategy, integrating GC/MS and LC/MS data, had been developed to study the protection function of herbal medicine, Fructus Ligustri Lucidi, on mouse liver. Mouse plasma samples were analyzed by GC/MS and LC/MS in conjunction with multiblock multivariate analysis method, multiblock partial least squares discriminant analysis (MBPLS-DA), to supply more important and distinct information of metabolomic biomarkers. Then, the biological pathway analysis was carried out to help further understanding the mechanism of liver protection of Fructus Ligustri Lucidi. © 2013 Springer-Verlag Berlin Heidelberg.

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