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Wei Q.,Nanjing Agricultural University | Wang Q.-Y.,Nanjing Agricultural University | Feng Z.-H.,Nanjing Agricultural University | Wang B.,Nanjing Agricultural University | And 3 more authors.
Plant Biotechnology Reports | Year: 2012

In order to explore a biotechnological method for improving potato tuber color and creating plants with increased anthocyanin contents, a potato UDP-glucose: flavonoid-3-O-glucosyltransferase (3GT) gene was inserted behind the GBSSI promoter of pBin19, and this construct was introduced into Solanum tuberosum L. cultivar Désirée plants by Agrobacterium-mediated transformation. Six independent transgenic lines overexpressing the 3GT gene were identified by PCR and Southern blot analysis from 18 kanamycin-resistant plants. Due to the expression of 3GT gene, the tuber color and the anthocyanin content were enhanced noticeably in the transgenic plants compared to the wild-type control plants. This result suggests that the 3GT gene can potentially be used to improve potato color and enhance levels of antioxidants in the diet. © 2011 Korean Society for Plant Biotechnology and Springer. Source


Lu Y.,Huaiyin Normal University | Lu Y.,Jiangsu Key Laboratory for Eco Agricultural Biotechnology around Hongze Lake | Xu H.,Huaiyin Normal University
Scientific World Journal | Year: 2014

Soil organic carbon fractions included microbial biomass carbon (MBC), dissolved organic carbon (DOC), and labile organic carbon (LOC), which was investigated over a 0-20 cm depth profile in three types of wetland in Hongze Lake of China. Their ecoenvironmental effect and the relationships with soil organic carbon (SOC) were analyzed in present experiment. The results showed that both active and SOC contents were in order reduced by estuarine wetland, flood plain, and out-of-lake wetland. Pearson correlative analysis indicated that MBC and DOC were positively related to SOC. The lowest ratios of MBC and DOC to SOC in the estuarine wetland suggested that the turnover rate of microbial active carbon pool was fairly low in this kind of wetland. Our results showed that estuarine wetland had a strong carbon sink function, which played important role in reducing greenhouse gas emissions; besides, changes of water condition might affect the accumulation and decomposition of organic carbon in the wetland soils. © 2014 Yan Lu and Hongwen Xu. Source


Guo J.-L.,Nanjing Agricultural University | Guo J.-L.,CAS Institute of Botany | Yu C.-L.,Nanjing Agricultural University | Fan C.-Y.,Nanjing Agricultural University | And 5 more authors.
Plant Cell, Tissue and Organ Culture | Year: 2010

Both tuberization and flowering are influenced by photoperiod in potato. TERMINAL FLOWER1 is a key regulator of floral timing in Arabidopsis and other herbaceous species. In order to clarify the relationship of TFL1 protein with tuberization, a homolog of TFL1, designated Solanum tuberosumTFL1 (StTFL1; accession no. DQ307621), was isolated from potato plantlets by reverse transcriptase-PCR and rapid amplification of cDNA ends. The predicted amino acid sequence of this cDNA had a high degree of identity with other homologous members of the phosphatidylethanolamine-binding protein family. Analysis of mRNA levels for StTFL1 showed that it was highly expressed in roots and initial stolons, with the expression becoming progressively weaker during subsequent tuberization. Over-expression of StTFL1 resulted in normal tuberization in transgenic lines under the long-day condition. These results demonstrate that StTFL1 is involved in the regulation of tuberization. © 2010 Springer Science+Business Media B.V. Source


Lu Y.,Huaiyin Normal University | Lu Y.,CAS Changchun Northeast Institute of Geography and Agroecology | Lu Y.,Jiangsu Key Laboratory for Eco Agricultural Biotechnology around Hongze Lake | Xu H.-W.,Huaiyin Normal University | Song C.-C.,CAS Changchun Northeast Institute of Geography and Agroecology
Journal of Food, Agriculture and Environment | Year: 2012

N 2O fluxes in field of Calamagrostis angustifolia meadow and Carex lasiocarpa marsh were measured using the technique of closed opaque chamber and gas chromatography system during 2003~2005 growing seasons. The experiment was carried out at the Sanjiang Mire Wetland Experimental Station in Northeast China. The results showed that wetland was weak N 2O emission source in growing seasons. The influence of wetland plants on N 2O emission of wetland ecosystem was not the same in different growing stages. N 2O emission flux reached peak value at heading, flowering and fructicative stage from Calamagrostis angustifolia meadow. This was related to rise in temperature and vigorous metabolism. N 2O emission flux reached maximum at seedling stages from Carex lasiocarpa marsh. Frozen soil freezing and thawing was the main reason. With the plants participation, the N 2O emissions ability was enhanced greatly. N 2O fluxes from soil-plant system were 1.6, 2.11 and 2.33 times as high as that from soil in Calamagrostis angustifolia meadow during three growing seasons, while 1.81, 1.5 and 1.36 times in Carex lasiocarpa marsh. The N 2O fluxes of Calamagrostis angustifolia meadow was higher than that of Carex lasiocarpa marsh during three growing seasons. The differences of soil physical and chemical characteristics and hydrology situations between the two wetlands could explain this phenomenon. Source


Lu Y.,Huaiyin Normal University | Lu Y.,Jiangsu Key Laboratory for Eco Agricultural Biotechnology around Hongze Lake | Lu Y.,Chinese Academy of Sciences
African Journal of Agricultural Research | Year: 2011

Sanjiang Plain is the largest freshwater marsh wetland in China. Carex lasiocarpa and Carex limosa are two dominant species in the area. The niche of C. limosa is similar with C. lasiocarpa and both species always distribute in the same region. However, the distribution area of C. limosa is far smaller than that of C. lasiocarpa. The aim of the study is to determine whether C. lasiocarpa had superiority in plant growth than C. limosa in the condition of submergence. To this end, plant growth and root morphologies were investigated in two Carex species. Experimental treatments included three water levels (0, 10 and 20 cm). Relative growth rates in C. lasiocarpa (0.038 g g -1 d-1) were much higher than that in C. limosa (0.030 g-1 d-1) under 20 cm water level. There existed more severe effects in biomass accumulations in C. limosa (dropped 77%) than that in C. lasiocarpa (dropped 52%) when comparing 20 cm water level to 0 cm water level. Enhanced water level could enhance root porosity (C. lasiocarpa 20.4% and C. limosa 21.5%) in both species but form shorter roots in C. limosa. Higher relative shoot growth rate could be found in C. lasiocarpa under deep water conditions. These data could explain why the two species had the same niche but different distribution areas in Sanjiang plain. ©2011 Academic Journals. Source

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