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Guo J.-L.,Nanjing Agricultural University | Guo J.-L.,CAS Institute of Botany | Yu C.-L.,Nanjing Agricultural University | Fan C.-Y.,Nanjing Agricultural University | And 5 more authors.
Plant Cell, Tissue and Organ Culture | Year: 2010

Both tuberization and flowering are influenced by photoperiod in potato. TERMINAL FLOWER1 is a key regulator of floral timing in Arabidopsis and other herbaceous species. In order to clarify the relationship of TFL1 protein with tuberization, a homolog of TFL1, designated Solanum tuberosumTFL1 (StTFL1; accession no. DQ307621), was isolated from potato plantlets by reverse transcriptase-PCR and rapid amplification of cDNA ends. The predicted amino acid sequence of this cDNA had a high degree of identity with other homologous members of the phosphatidylethanolamine-binding protein family. Analysis of mRNA levels for StTFL1 showed that it was highly expressed in roots and initial stolons, with the expression becoming progressively weaker during subsequent tuberization. Over-expression of StTFL1 resulted in normal tuberization in transgenic lines under the long-day condition. These results demonstrate that StTFL1 is involved in the regulation of tuberization. © 2010 Springer Science+Business Media B.V.


Han M.,Jiangsu Key Laboratory for Eco Agricultural Biotechnology around Hongze Lake | Wang W.,Huaian Institute of Supervision and Inspection on Product Quality | Wang X.,Jiangsu Key Laboratory for Eco Agricultural Biotechnology around Hongze Lake | Liu X.,Jiangsu Key Laboratory for Eco Agricultural Biotechnology around Hongze Lake | And 3 more authors.
Applied Biochemistry and Biotechnology | Year: 2015

N-glycosylation usually occurs at the Asn-Xaa-Ser/Thr sequon of glycoproteins in Pichia pastoris, exerting great effects on expression efficiency; however, Asn-Xaa-Thr is more efficiently glycosylated than Asn-Xaa-Ser. In this study, the role of the two sequons in the expression of recombinant elastase (rPAE) was investigated. At N43, N212, and N280 of rPAE, Asn-Xaa-Thr was substituted for the native Asn-Xaa-Ser sequon through site-directed mutagenesis, and the two sequon forms were introduced into rPAE at N36 and N264. As expected, substitution at N36, N43, N212, and N280 enhanced the degree of N-glycosylation. At N212 or N280, substitution increased rPAE production effectively by 43 and 25 %, respectively. In comparison, at N36, N43, and N264, the change inhibited rPAE expression to varying extents; specifically, substitution at N36 resulted in a 31 % decrease, while substitution at N43 or N264 resulted in a decrease of less than 9 %. It is suggested that the effect of the substitution of Asn-Xaa-Thr for Asn-Xaa-Ser on rPAE expression is roughly related to the role of the original Asn-Xaa-Ser sequon. As the conversion of Ser to Thr at N-glycosylation sites through site-directed mutagenesis is easily achieved, it is a feasible means of improving the expression of recombinant proteins in P. pastoris. © 2014, Springer Science+Business Media New York.


Yang W.,Huaiyin Normal University | Yang W.,Jiangsu Key Laboratory for Eco Agricultural Biotechnology Around Hongze Lake | Jiang G.,Jiangsu Key Laboratory for Eco Agricultural Biotechnology Around Hongze Lake | Wang Z.,Huaian Environmental Monitoring Center | And 3 more authors.
Acta Geographica Sinica | Year: 2012

Sorption behavior and adsorption-desorption equilibrium mass concentration of phosphorus on surface sediments samples taken from Hongze Lake at nine sites were determined in laboratory, the correlation between chemical-physical properties of sediments and their phosphorus sorption characteristics were analyzed. Under low mass concentration conditions, the maximum phosphorus sorption capacities were 50.67-85.17 mg/kg, and there was a negative sorption process during phosphate sorption on surface sediments. The zero equilibrium P concentration (EPC 0) of the sediments ranged from 0.06 to 0.11 mg/L, less than the level for the overlying water, showing a positive phosphate adsorption on surface sediments. The maximum phosphate adsorption, NAP and the total maximum phosphate adsorption were positively correlated with active iron and active aluminous contents in the sediments, but had poor correlation with total P, organic P, inorganic P and organic matter in the sediments. The zero equilibrium P concentration (EPC 0) had poor correlation with chemical-physical parameters of surface sediments.


Wei Q.,Nanjing Agricultural University | Wang Q.-Y.,Nanjing Agricultural University | Feng Z.-H.,Nanjing Agricultural University | Wang B.,Nanjing Agricultural University | And 3 more authors.
Plant Biotechnology Reports | Year: 2012

In order to explore a biotechnological method for improving potato tuber color and creating plants with increased anthocyanin contents, a potato UDP-glucose: flavonoid-3-O-glucosyltransferase (3GT) gene was inserted behind the GBSSI promoter of pBin19, and this construct was introduced into Solanum tuberosum L. cultivar Désirée plants by Agrobacterium-mediated transformation. Six independent transgenic lines overexpressing the 3GT gene were identified by PCR and Southern blot analysis from 18 kanamycin-resistant plants. Due to the expression of 3GT gene, the tuber color and the anthocyanin content were enhanced noticeably in the transgenic plants compared to the wild-type control plants. This result suggests that the 3GT gene can potentially be used to improve potato color and enhance levels of antioxidants in the diet. © 2011 Korean Society for Plant Biotechnology and Springer.


Fan C.-Y.,Nanjing Agricultural University | Yin J.-M.,Nanjing Agricultural University | Wang B.,Nanjing Agricultural University | Zhang Y.-F.,Nanjing Agricultural University | And 2 more authors.
Agricultural Sciences in China | Year: 2010

A homologue of flowering locus T gene, designated StFT, was isolated from Solanum tuberosum by reverse transcriptase-polymerase chain reaction (accession no. GU223211). The DNA sequence of StFT was 1 626 bp long and contained four exons and three introns. The open reading frame of the gene was 522 bp long and encoded a putative protein of 173 amino acids with a molecular weight of 19.75 kD and a theoretical pI of 7.76. StFT protein had a conserved PBP domain and a higher degree of identity with FT homologous members from other species. Analysis on the mRNA levels of StFT showed that it was highly expressed in leaves, apical buds, flowers, and swelling stolons. Further analysis indicated that its expression was regulated by CONSTANS gene in StCOL-antisense transgenic potato plants. © 2010 Chinese Academy of Agricultural Sciences.


Lu Y.,Huaiyin Normal University | Lu Y.,Jiangsu Key Laboratory for Eco Agricultural Biotechnology Around Hongze Lake | Xu H.,Huaiyin Normal University
Scientific World Journal | Year: 2014

Soil organic carbon fractions included microbial biomass carbon (MBC), dissolved organic carbon (DOC), and labile organic carbon (LOC), which was investigated over a 0-20 cm depth profile in three types of wetland in Hongze Lake of China. Their ecoenvironmental effect and the relationships with soil organic carbon (SOC) were analyzed in present experiment. The results showed that both active and SOC contents were in order reduced by estuarine wetland, flood plain, and out-of-lake wetland. Pearson correlative analysis indicated that MBC and DOC were positively related to SOC. The lowest ratios of MBC and DOC to SOC in the estuarine wetland suggested that the turnover rate of microbial active carbon pool was fairly low in this kind of wetland. Our results showed that estuarine wetland had a strong carbon sink function, which played important role in reducing greenhouse gas emissions; besides, changes of water condition might affect the accumulation and decomposition of organic carbon in the wetland soils. © 2014 Yan Lu and Hongwen Xu.


Lu Y.,Huaiyin Normal University | Lu Y.,Jiangsu Key Laboratory for Eco agricultural Biotechnology around Hongze Lake | Lu Y.,Chinese Academy of Sciences
African Journal of Agricultural Research | Year: 2011

Sanjiang Plain is the largest freshwater marsh wetland in China. Carex lasiocarpa and Carex limosa are two dominant species in the area. The niche of C. limosa is similar with C. lasiocarpa and both species always distribute in the same region. However, the distribution area of C. limosa is far smaller than that of C. lasiocarpa. The aim of the study is to determine whether C. lasiocarpa had superiority in plant growth than C. limosa in the condition of submergence. To this end, plant growth and root morphologies were investigated in two Carex species. Experimental treatments included three water levels (0, 10 and 20 cm). Relative growth rates in C. lasiocarpa (0.038 g g -1 d-1) were much higher than that in C. limosa (0.030 g-1 d-1) under 20 cm water level. There existed more severe effects in biomass accumulations in C. limosa (dropped 77%) than that in C. lasiocarpa (dropped 52%) when comparing 20 cm water level to 0 cm water level. Enhanced water level could enhance root porosity (C. lasiocarpa 20.4% and C. limosa 21.5%) in both species but form shorter roots in C. limosa. Higher relative shoot growth rate could be found in C. lasiocarpa under deep water conditions. These data could explain why the two species had the same niche but different distribution areas in Sanjiang plain. ©2011 Academic Journals.


Lu Y.,Huaiyin Normal University | Lu Y.,CAS Changchun Northeast Institute of Geography and Agroecology | Lu Y.,Jiangsu Key Laboratory for Eco agricultural Biotechnology around Hongze Lake | Xu H.-W.,Huaiyin Normal University | Song C.-C.,CAS Changchun Northeast Institute of Geography and Agroecology
Journal of Food, Agriculture and Environment | Year: 2012

N 2O fluxes in field of Calamagrostis angustifolia meadow and Carex lasiocarpa marsh were measured using the technique of closed opaque chamber and gas chromatography system during 2003~2005 growing seasons. The experiment was carried out at the Sanjiang Mire Wetland Experimental Station in Northeast China. The results showed that wetland was weak N 2O emission source in growing seasons. The influence of wetland plants on N 2O emission of wetland ecosystem was not the same in different growing stages. N 2O emission flux reached peak value at heading, flowering and fructicative stage from Calamagrostis angustifolia meadow. This was related to rise in temperature and vigorous metabolism. N 2O emission flux reached maximum at seedling stages from Carex lasiocarpa marsh. Frozen soil freezing and thawing was the main reason. With the plants participation, the N 2O emissions ability was enhanced greatly. N 2O fluxes from soil-plant system were 1.6, 2.11 and 2.33 times as high as that from soil in Calamagrostis angustifolia meadow during three growing seasons, while 1.81, 1.5 and 1.36 times in Carex lasiocarpa marsh. The N 2O fluxes of Calamagrostis angustifolia meadow was higher than that of Carex lasiocarpa marsh during three growing seasons. The differences of soil physical and chemical characteristics and hydrology situations between the two wetlands could explain this phenomenon.


Gao J.,Huaiyin Institute of Technology | Gao J.,Jiangsu Key Laboratory for Eco Agricultural Biotechnology around Hongze Lake | Zhou H.,Huaian Wanbang Aromatic Chemicals Industry Co.
Advanced Materials Research | Year: 2013

6 congeners of phthalate esters (PAEs) were analyzed in soils with various land use types from the area around Hongze Lake, the fourth largest freshwater lake in China. The total concentrations of 6 congeners (Σ6PAEs) were 5.070-2.067, 0.723-2.050, 0.331-1.645, 0.002-0.173 mg kg-1 in surface soil from plastic film greenhouse (PG), conventional vegetable fields (CV), plastic film greenhouse-rice rotated fields (PR), and uncultured land (UL), respectively. DnBP and DEHP were dominant species in PAEs averagely accounted for 46.4% and 47.0% of the Σ6PAEs concentrations in all surface soils. And congener percent composition of PAEs in surface soils from uncultured land was considerably different from cultured soils. Vertical distribution of PAEs in soil profile was influenced by land use type, congener species, and the content of congener in surface soil, etc. However, the amounts of PAEs in surface soil accounted more than 58.5% of total amounts in soils. In addition, no significant relationship was observed between total organic C or pH and the concentrations of PAEs in surface soil. The contents of PAEs in soils from the area around Hongze Lake were related with land use types, especially with the use of plastic film. And the general contamination level in soil was comparable or lower than those present in other areas. According to our knowledge, this is the first study to examine the present status of PAEs in soils from the area around Hongze Lake. © (2013) Trans Tech Publications, Switzerland.

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