Jiangsu Key Laboratory for Biomass based Energy and Enzyme Technology

Huaian, China

Jiangsu Key Laboratory for Biomass based Energy and Enzyme Technology

Huaian, China
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Wu S.,China Agricultural University | Gu W.,China Agricultural University | Pang C.,China Agricultural University | Dong R.,China Agricultural University | Deng Y.,Jiangsu Key Laboratory for Biomass Based Energy and Enzyme Technology
Nongye Jixie Xuebao/Transactions of the Chinese Society for Agricultural Machinery | Year: 2013

Lack of buffering capacity of fermentation substrate in anaerobic digestion may easily lead to acidification. Alkalinity is a key indicator for measuring buffering capacity of the fermentation substrate, so its online monitoring should be enhanced to keep the anaerobic fermentation process stable automatically. An online monitoring system based on joint titration of alkalinity and VFA was designed. The software system was introduced in detail. The system could determine alkalinity and VFA simultaneously, which achieved real-time curve display and pH meter calibration. Moreover, automatic analysis could be realized. The experiment results showed that the system had the ability of the rapid determination of alkalinity and VFA in the fermentation process. The relative standard deviation was less than 5%, which was lower than the manual titration and indicated a high measurement accuracy of the proposed system.


Yan Q.,Jiangnan University | Yan Q.,Harbin Institute of Technology | Yan Q.,Jiangsu Key Laboratory for Biomass based Energy and Enzyme Technology | Li Y.,Jiangnan University | And 5 more authors.
Journal of Hazardous Materials | Year: 2012

Enhanced biomethanation with acid stress on anaerobic sludge, dehydrogenase activity, protein expression, and the primary proteomic profiling of microbial communities during the enhanced anaerobic digestion process from Taihu Blue Algae were investigated. It was found that the accumulation of organic acids and the specific biogas accumulation rate were 1.8 and 1.3 times of the control, when 10. g/L and 7.5. g/L of butyrate were selected for acid stress, respectively. Meanwhile, dehydrogenase activity of the 7.5. g/L acid stress group exhibited an increase of 1.6 times of the control, and protein expression was also found to be enhanced sharply as revealed by 1D-PAGE. Finally, twenty of the matched protein spots through 2D-PAGE from both the control and the 7.5. g/L stress groups were identified by MALDI-TOF MS, and five of which were proved to be involved in bioenergy metabolism. Significantly, ATR related proteins might be induced as the p. Is of which were acidic as 5.92, 5.51 and 5.54, respectively. © 2012 Elsevier B.V.


You Q.,Huaiyin Institute of Technology | You Q.,Jiangsu Key Laboratory for Biomass based Energy and Enzyme Technology | You Q.,Nanjing University | Yin X.,Huaiyin Institute of Technology | And 2 more authors.
Journal of Food, Agriculture and Environment | Year: 2013

Ultrasonic technology was applied for polysaccharides extraction from the fruiting body of Tricholoma matsutake and response surface methodology (RSM) was used to optimize the effects of processing parameters on polysaccharides yields. On the basis of the results of a single-factors test, three independent and main variables, including extraction time (X1: 130-170 s), ultrasonic power (X2: 340-380 W) and ratio of water to raw material (X3: 45-55 ml/g) were studied by Box-Behnken design. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also examined using the appropriate statistical methods. The best extraction conditions are as follows: extraction time 160 s, ultrasonic power 365 W and ratio of water to raw material 53.5 ml/g. Under the optimization conditions, the experimental yield of polysaccharides was 7.97 ± 0.31%, which was agreed closely with the predicted value (7.95%).


You Q.,Huaiyin Institute of Technology | You Q.,Jiangsu Key Laboratory for Biomass based Energy and Enzyme Technology | You Q.,Nanjing University | Yin X.,Huaiyin Institute of Technology | And 5 more authors.
Carbohydrate Polymers | Year: 2014

Ultrasonic-microwave synergistic extraction (UMSE), purification, and characterization of Tricholoma mongolicum Imai polysaccharides (TMIPs) from fruit bodies were investigated in this study. Response surface methodology (RSM) was employed to optimize the UMSE conditions. The results indicated that the optimal extraction conditions were as follows: extraction time of 24.65 min, microwave power of 109.98 W, and water to raw material ratio of 21.62 ml/g. Under optimized conditions, the yield and purity of TMIPs were 35.41 ± 0.62% and 73.92 ± 0.83%, respectively. Crude TMIPs were purified by DEAE-Cellulose 52 chromatography and Sephadex G-100 chromatography to afford four fractions, namely, TMIP-1, TMIP-2, TMIP-3, and TMIP-4. Preliminary TMIP characterization was conducted by Fourier transform infrared spectroscopy. TMIP antioxidant activities were investigated by measuring its scavenging ability on 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radicals, ferric reducing activity power, and reducing power assay. The results indicated that TMIPs have good antioxidant activity. © 2013 Elsevier Ltd. All rights reserved.


You Q.,Huaiyin Institute of Technology | You Q.,Jiangsu Key Laboratory for Biomass based Energy and Enzyme Technology | You Q.,Nanjing University | Yin X.,Huaiyin Institute of Technology | And 3 more authors.
International Journal of Medicinal Mushrooms | Year: 2014

Ultrasound-assisted extraction, purifcation, and characterization of antioxidant polysaccharides from Tricholoma matsutake were carried out. On the basis of the results of a single-factor test, three independent and main variables, including extraction time (X1: 130-170 s), ultrasonic power (X2: 340-380 W), and ratio of water to raw material (X3: 45-55 mL/g) were studied by Box-Behnken design. The optimum extraction conditions are as follows: extraction time 160 s, ultrasonic power 365 W, and ratio of water to raw material 53.5 mL/g. Under the optimized conditions, the yield of T. matsutake polysaccharides (TMP) was 7.97±0.31%. The crude TMP was purifed by DEAE-cellulose 52 chromatography and Sephadex G-100 chromatography to afford two fractions, TMP-1 and TMP-2. The crude TMP contained 85.76% carbohydrates, 3.57% proteins, and 0.13% uronic acids. The constituent monosaccharides were predominantly glucose, galactose, mannose, xylose, fucose, and glucuronic acid residues. © 2014 Begell House, Inc.


You Q.,Huaiyin Institute of Technology | You Q.,Jiangsu Key Laboratory for Biomass based Energy and Enzyme Technology | Yin X.,Huaiyin Institute of Technology | Yin X.,Jiangsu University | And 2 more authors.
Bioresource Technology | Year: 2013

Lipase from Burkholderia cepacia was immobilized on modified attapulgite by cross-linking reaction for biodiesel production with jatropha oil as feedstock. Effects of various factors on biodiesel production were studied by single-factor experiment. Results indicated that the best conditions for biodiesel preparation were: 10g jatropha oil, 2.4g methanol (molar ratio of oil to methanol is 1:6.6) being added at 3h intervals, 7 wt% water, 10 wt% immobilized lipase, temperature 35°C, and time 24h. Under these conditions, the maximum biodiesel yield reached 94%. The immobilized lipase retained 95% of its relative activity during the ten repeated batch reactions. The half-life time of the immobilized lipase is 731h. Kinetics was studied and the Vmax of the immobilized lipases were 6.823mmolL-1. This immobilized lipase catalyzed process has potential industrial use for biodiesel production to replace chemical-catalyzed method. © 2013 Elsevier Ltd.


You Q.,Huaiyin Institute of Technology | You Q.,Jiangsu Key Laboratory for Biomass based Energy and Enzyme Technology | Yin X.,Huaiyin Institute of Technology | Yin X.,Jiangsu University | Zhao Y.,Huaiyin Institute of Technology
Carbohydrate Polymers | Year: 2013

Process of enzyme assisted extraction (EAE) of polysaccharides from Cornus officinalis was optimized by response surface methodology (RSM). The influence of four different factors on the yield of C. officinalis polysaccharides (COP) was studied. Results showed that the optimal conditions were compound enzyme amount of 2.15%, extraction pH of 4.2, extraction temperature of 55 °C and extraction time of 97 min. Under these conditions, the COP yield was 9.29 ± 0.31%, which was well in agreement with the value predicted by the model. The three methods, EAE, hot water extraction (HWE), ultrasound-assisted extraction (UAE) for extracting COP by RSM were further compared. Results showed that EAE had the largest yield of polysaccharides with lower equipment cost. © 2013 Elsevier Ltd. All rights reserved.

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