Jiangsu Kanon Pharmaceutical Co.

Xinpu, China

Jiangsu Kanon Pharmaceutical Co.

Xinpu, China
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Li L.,State Key Laboratory of New Technology for Chinese Medicine Pharmaceutical Process | Li L.,Shenyang Pharmaceutical University | Wang Z.,State Key Laboratory of New Technology for Chinese Medicine Pharmaceutical Process | Wang Z.,Jiangsu Kanon Pharmaceutical Co. | And 8 more authors.
Journal of Chromatographic Science | Year: 2015

A high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry-UV-irradiation (HPLC-QTOF-MS-UV) method was established for rapid separation and structural identification of the constituents in Re Du Ning Injections (RDNI). A total of 20 potentially bioactive compounds including 10 caffeoylquinic acids and 10 iridoid glycosides were identified or tentatively characterized in RDNI by comparing their retention times and MS spectra with those of authentic standards or literature data. In particular, UV-irradiation was employed in the identification of the cis/trans isomers of caffeoylquinic acids. Furthermore, each compound was assigned to the individual raw materials (Artemisia annua L., Lonicera japonica Thunb. or Gardenia jasminoides Ellis) present in RDNI. This is the first time that an HPLC-QTOF-MS-UV analytical method has been used for the identification of caffeoylquinic acids in RDNI. © The Author 2014. Published by Oxford University Press.


Liu Z.-X.,Shenyang Pharmaceutical University | Liu C.-T.,Shenyang Pharmaceutical University | Liu Q.-B.,Shenyang Pharmaceutical University | Ren J.,Changzhou University | And 4 more authors.
Journal of Functional Foods | Year: 2015

The flower buds of Lonicera japonica are commonly used as popular healthy beverages that are also effective in the prevention and cure of a variety of chronic diseases, such as diabetes, and also as an anti-inflammatory treatment for thousands of years. In this study, three new iridoid glycosides along with twenty-five known analogues were isolated from the aqueous extract of L. japonica. Their structures were determined by extensive 1D NMR, 2D NMR and HR-ESIMS spectroscopic data analyses, and all the isolates were investigated with regard to lipopolysaccharide (LPS)-induced nitric oxide (NO) production and α-glucosidase inhibitory effects. Among these compounds, 12 and 13 exhibited strong NO inhibitory activities and no cytotoxic effects. Moreover, most compounds were potent α-glucosidase inhibitors compared with the positive control acarbose. These findings will enhance knowledge about the bioactive components of L. japonica and help promote its potential application in functional foods. © 2015 Elsevier Ltd.


Wang Y.-H.,Peking University | Wang Y.-H.,Peking Union Medical College | Yu H.-T.,Jiangsu Kanon Pharmaceutical Co. | Pu X.-P.,Peking University | Du G.-H.,Peking Union Medical College
Molecules | Year: 2013

Parkinson's disease (PD) is a neurodegenerative disorder characterized by progressive loss of dopaminergic (DA) neurons at the substantia nigra. Mitochondrial dysfunction is involved in the mechanism of cell damage in Parkinson's disease (PD). 6-Hydroxydopamine (6-OHDA) is a dopamine analog which specifically damages dopaminergic neurons. Baicalein has been previously reported to have potential in the treatment of PD. The purpose of the present study was to investigate the mechanism of action of baicalein against 6-OHDA injury in SH-SY5Y cells. The results showed that baicalein significantly alleviated alterations of mitochondrial redox activity and mitochondrial membrane potential induced by 6-OHDA in a dose-dependent manner in SH-SY5Y cells compared with vehicle group. Futhermore, baicalein decreased the production of ROS and upregulated the DJ-1 protein expression in SH-SY5Y cells. In addition, baicalein also inhibited ROS production and lipid peroxidation (IC50 = 6.32 ± 0.03 μM) in rat brain mitochondia. In summary, the underlying mechanisms of baicalein against 6-OHDA-induced mitochondrial dysfunction may involve inhibition of mitochondrial oxidation and upregulation of DJ-1 protein expression. © 2013 by the authors; licensee MDPI, Basel, Switzerland.


Wang Y.-H.,Peking Union Medical College | Wang Y.-H.,Peking University | Han Y.-P.,Peking Union Medical College | Yu H.-T.,Jiangsu Kanon Pharmaceutical Co. | And 2 more authors.
European Journal of Pharmacology | Year: 2014

Methylglyoxal (MGO), a glucose derived dicarbonyl intermediate, is a major precursor of advanced glycation end products (AGEs) which have been linked to the development of diabetic cataract. Protocatechualdehyde (PCA), a phenolic acid compound, is found in the roots of Salvia miltiorrhiza. This study was to investigate the effect of PCA against MGO-induced cytotoxicity in human lens epithelial cells (SRA01/04 cells) and the possible involved molecular mechanism. The results showed that PCA alleviated MGO-induced mitochondrial dysfunction and apoptosis in SRA01/04 cells. Furthermore, PCA was capable of inhibiting MGO-mediated AGEs formation and blocking receptor of AGEs expression in SRA01/04 cells. It is concluded that PCA could be useful in attenuation of MGO-induced cell damage and the possible mechanism is involved in modulating AGEs-receptor of AGEs axis in human lens epithelial cells, which suggests that PCA has a potential protective effect on diabetic cataract. © 2014 Elsevier B.V. All rights reserved.


Wang Y.-H.,Peking University | Wang Y.-H.,Peking Union Medical College | Yu H.-T.,Jiangsu Kanon Pharmaceutical Co. | Pu X.-P.,Peking University | Du G.-H.,Peking Union Medical College
Journal of Molecular Neuroscience | Year: 2014

Advanced glycation end products (AGEs) have been identified in age-related intracellular protein deposits of neurodegenerative diseases. Methylglyoxal (MGO), a dicarbonyl metabolite, is a major precursor of AGEs which have been linked to the development of neurodegenerative diseases. Myricitrin, a flavanoid isolated from the root bark of Myrica cerifera, attenuated 6-OHDA-induced mitochondrial dysfunction and had a potential anti-Parkinson's disease in our previous investigation. The aims of this study were to investigate the protective effects of myricitrin against MGO-induced injury in SH-SY5Y cells and also to look for the possible mechanisms. The results showed that exposure of SH-SY5Y cells to MGO caused decreases of cell viability, intracellular ATP, mitochondrial redox activity, and mitochondrial membrane potential and an increase in reactive oxygen species generation. However, these mitochondrial dysfunctions were alleviated by co-treatment with myricitrin. Additionally, myricitrin was capable of inhibiting AGEs formation, blocking RAGE expression, and inhibiting NF-κB activation and translocation triggered by MGO in SH-SY5Y cells. Our results suggest that myricitrin alleviates MGO-induced mitochondrial dysfunction, and the possible mechanism is through modulating the AGEs/RAGE/NF-κB pathway. In summary, myricitrin might offer a promising therapeutic strategy to reduce the neurotoxicity of reactive dicarbonyl compounds, providing a potential benefit agent with age-related neurodegenerative diseases. © 2014 Springer Science+Business Media.


PubMed | Jiangsu Kanon Pharmaceutical Co., Peking University, Chinese Institute of Materia Medica and Peking Union Medical College
Type: | Journal: European journal of pharmacology | Year: 2014

Methylglyoxal (MGO), a glucose derived dicarbonyl intermediate, is a major precursor of advanced glycation end products (AGEs) which have been linked to the development of diabetic cataract. Protocatechualdehyde (PCA), a phenolic acid compound, is found in the roots of Salvia miltiorrhiza. This study was to investigate the effect of PCA against MGO-induced cytotoxicity in human lens epithelial cells (SRA01/04 cells) and the possible involved molecular mechanism. The results showed that PCA alleviated MGO-induced mitochondrial dysfunction and apoptosis in SRA01/04 cells. Furthermore, PCA was capable of inhibiting MGO-mediated AGEs formation and blocking receptor of AGEs expression in SRA01/04 cells. It is concluded that PCA could be useful in attenuation of MGO-induced cell damage and the possible mechanism is involved in modulating AGEs-receptor of AGEs axis in human lens epithelial cells, which suggests that PCA has a potential protective effect on diabetic cataract.

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