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Wang Y.-Y.,Jilin University | Zhu Q.-S.,Jilin University | Wang Y.-W.,Jiangsu Jiankang Vocational College | Yin R.-F.,Jilin University
Chinese Medical Journal | Year: 2015

Background: Thymosin beta-4 (TB-4) is considered key roles in tissue development, maintenance and pathological processes. The study aimed to prove TB-4 positive biological function on nucleus pulposus (NP) cell apoptosis and slowing the process of cell aging while increasing the cell proliferation. Methods: TB-4 recombinant adeno-associated virus (AAV) was constructed and induced to human NP cells. Cell of same group were cultured without gene modification as controlled group. Proliferation capacity and cell apoptosis were observed during 6 passages of the cells. Morphology and expression of the TB-4 gene were documented as parameter of cell activity during cell passage. Results: NP cells with TB-4 transfection has normal TB-4 expression and exocytosis. NP cells with TB-4 transfection performed significantly higher cell activity than that at the control group in each generation. TB-4 recombinant AAV-transfected human NP cells also show slower cell aging, lower cell apoptosis and higher cell proliferation than control group. Conclusions: TB-4 can prevent NP cell apoptosis, slow NP cell aging and promote NP cell proliferation. AAV transfection technique was able to highly and stably express TB-4 in human NP cells, which may provide a new pathway for innovation in the treatment of intervertebral disc degenerative diseases. © 2015, Chinese Medical Association. All rights reserved.

Huang H.,Sun Yat Sen University | Ma R.,Jiangsu Jiankang Vocational College | Liu D.,Sun Yat Sen University | Liu C.,Sun Yat Sen University | And 4 more authors.
Disease Markers | Year: 2012

Objective: The purpose of the present study was to investigate the value of ox-LDL and oxidation ratio of LDL (ox-LDL/TC, ox-LDL/HDL-C and ox-LDL/LDL-C) in diagnosis and prognosis evaluation in CAD patients. Also, we aimed to observe the effect of statins on reducing level of ox-LDL and oxidation ratio of LDL, and explore whether statins still have similar effect on ox-LDL in a short period of therapy (within 2 weeks). Methods: Blood ox-LDL, TC, HDL-C, LDL-C, and TG were measured in cases with acute myocardial infarction (AMI, n=177), unstable angina pectoris (UAP, n=195), stable angina pectoris (SAP, n=228), normal control (n=120), and high risk control (n=140). Results: Mean value of ox-LDL and oxidation ratio of LDL was significantly higher in the CAD group than in the two control groups. The AUC of ROC curve of ox-LDL, ox-LDL/TC, ox-LDL/HDL-C, ox-LDL/LDL-C and apoA1/apoB were more than 0.50 (P < 0.001). Multivariate logistic regression analysis showed that age and ox-LDL/LDL-C related with short-term, while ox-LDL/LDL-C and ox-LDL/TC related with long-term prognosis (p < 0.05). Furthermore, after treatment with statins for 2 weeks, TC, LDL-C, ox-LDL, ox-LDL/TC, ox-LDL/HDL-C and ox-LDL/LDL-C decreased by 22%, 28%, 38%, 29%, 23% and 25% respectively. And the reduction of ox-LDL by statins is independent of lowering of LDL-C and TC. Conclusions: Ox-LDL and oxidation ratio of LDL are closely related with AS, and they are better biomarkers for discriminating between patients with coronary artery disease and healthy subjects. In addition, statins can decrease level of ox-LDL significantly, which is independent of lowering of LDL-C and TC. © 2012-IOS Press and the authors. All rights reserved.

Wu P.,Jiangsu Jiankang Vocational College | Tang R.-N.,Nanjing Southeast University | Zou J.-H.,Nanjing Southeast University | Wang F.-C.,Nanjing Southeast University
Hepato-Gastroenterology | Year: 2012

Background/Aims: Disseminated tumor cells (DTCs) have been associated with clinical outcome in various malignancies. The aim of this study is to examine the status of DTC in peripheral blood (PB) and bone marrow (BM) of colorectal cancer (CRC) and to evaluate its clinical significance. Methodology: A total of 75 CRC patients treated with radical resection were enrolled in this study. Nested RT-PCR was used to detect the 2 representative markers of DTC, carcinoembryonic antigen (CEA) and survivin. Results: The frequencies of DTC detected in PB and BM were 52.0% (39/75) and 29.3% (22/75) respectively, and showed no correlations with each other (p>0.05), their combination increased the sensitivity to 65.5% (49/75]. Furthermore, DTC positive either in PB or BM was correlated with lymph metastasis, advanced stage and adverse 2-year progression free survival (PFS). In a multivariate analysis using the Cox proportional hazard model, DTC positive in PB and/or BM was an independent unfavorable prognostic factor for CRC apart from lymph metastasis and adjuvant chemotherapy. Conclusions: CEA and/or survivin-positive DTCs may be a promising biomarker for prognosis assessment in CRC. © H.G.E. Update Medical Publishing S.A.

Chang L.,Nanjing Southeast University | Wu P.,Jiangsu Jiankang Vocational College | Senthilkumar R.,Nanjing Southeast University | Tian X.,Nanjing Southeast University | And 4 more authors.
Journal of Cancer Research and Clinical Oncology | Year: 2016

Purpose: Altered cellular metabolism has received increased attention as an important hallmark of cancer. Activation of FASN has been found to be involved in many human tumors. Despite extensive research in FASN function on cancer, the underlying mechanism is not entirely understood yet. Methods: Cerulenin was used to suppress the FASN expression in human colorectal cancer cell lines (HT29 and LoVo). Expression of PI3K, Akt, p-Akt, mTOR, p-mTOR, FASN, and AZGP1 was measured using western blotting and qPCR. ATP and lactic acid were assessed to investigate the activation of energy metabolism. Cell cytotoxicity assay was studied by cell counting kit-8 assay. The capacity of cell proliferation and migration was investigated by clonogenic and invasion assay. Analysis of apoptosis and the cell cycle was detected by flow cytometry. Results: We found that the expression of FASN was down-regulated, while the expression of PI3K, p-Akt, p-mTOR, and AZGP1 was down-regulated in HT29 and LoVo cells treated with FASN inhibitor. Proliferation was reduced in FASN inhibitor-treated cells, which is consistent with an increased apoptosis rate. Furthermore, the migration of FASN inhibitor-treated cells was decreased and the content of ATP and lactic acid was also dropped. Conclusion: These findings suggest that inhibited FASN suppresses the malignant phenotype of colorectal cancer cells by down-regulating energy metabolism and mTOR signaling pathway. The results have paved the way to understand the relations of FASN, mTOR signaling pathway, and energy metabolism in colorectal cancer cells. © 2015, Springer-Verlag Berlin Heidelberg.

Wu P.,Nanjing University of Traditional Chinese Medicine | Wu P.,Jiangsu Jiankang Vocational College | Huang R.,Nanjing University of Traditional Chinese Medicine | Xiong Y.-L.,Nanjing University of Traditional Chinese Medicine | Wu C.,Nanjing University of Traditional Chinese Medicine
Chinese Journal of Natural Medicines | Year: 2016

Recent research has demonstrated that advanced liver fibrosis in patients could be reversed, but no approved agents are available for the treatment and prevention of liver fibrosis in humans. Curcumin (CUR) is the principal curcuminoid of turmeric. Inhibitory effects of CUR and its underlying mechanisms in liver fibrogenesis have been explored. In the present study, we hypothesized that epigenetic mechanisms contribute to the protective effects of CUR against liver fibrosis. We used CCl4-induced liver injury in BALB/c mice and the rat hepatic stellate cell line HSC-T6 as experimental models. Genomic DNA methylation was analyzed by MeDIP-chip and verified by real-time PCR on MeDIP-enriched DNA. The mRNA and protein expressions of DNMT1, α-SMA, and Col1α1 were determined by real-time PCR and Western blotting, respectively. The methylation statuses of FGFR3, FZD10, Gpx4, and Hoxd3 were further confirmed by quantitative methylation-specific PCR (qMSP). Our results showed that CUR treatment reversed liver injury in vivo and in vitro, possibly through down regulation of DNMT1, α-SMA, and Col1α1 and by demethylation of the key genes. In conclusion, aberrant methylation is closely associated with liver fibrosis and CUR treatment may reverse liver fibrosis by epigenetic mechanisms. © 2016 China Pharmaceutical University.

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