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Tan X.,Nanjing Medical University | Tan X.,Zhongshan Ophthalmic Center | Tan X.,Jiangsu Institute of Nuclear Medicine | Tan X.,University of Sydney
Eye (London, England) | Year: 2014

PURPOSE: To determine the levels of Th17-associated cytokines, particularly interleukin (IL)-17 and IL-22 in tears of patients with dry eye syndrome.METHODS: Tear samples were collected from 20 healthy volunteers, 20 dry eye (DE) patients with non-Sjögren's syndrome (NSSDE) and 20 DE patients with Sjögren's syndrome (SSDE). Symptom questionnaire was self-administered and multiple dry eye disease (DED)-related clinical tests were performed. The levels of IL-17 and IL-22 in tears were measured by enzyme-linked immunosorbent assay.RESULTS: The levels of IL-17 and IL-22 were significantly increased in tears of DE patients compared with those of controls and also higher in SSDE patients compared with those of NSSDE patients (P<0.05). Moreover, the levels of IL-17 and IL-22 were positively correlated with questionnaire score and keratopathy score but negatively correlated with tear film break-up time and Schirmer I test in both NSSDE and SSDE patients (P<0.05).CONCLUSIONS: The levels of IL-17 and IL-22 in tears were significantly increased in DE patients, which were associated with the disease severity. Therefore, Th17 cell-associated cytokines, particularly IL-17 and IL-22, may have important roles in the immunopathogenesis of the DED. Source

Zhang J.,Jiangnan University | Zhang J.,Jiangsu Institute of Nuclear Medicine | Liu Y.-J.,Jiangnan University | Park H.-S.,Gyeongsang National University | And 2 more authors.
Carbohydrate Polymers | Year: 2012

Water-soluble extracellular polysaccharides are known to possess weak or no in vitro antitumor activity. In this experiment, a mixture of extracellular Ganoderma lucidum polysaccharides (GLP) from the submerged fermentation broth was sulfated and studied on their antitumor activity. The sulfated GLP performed significant inhibition on the proliferation of assayed carcinoma cells in a dose-dependent manner, and present a degree of substitution-dependent suppressing to HepG2 cells. Meanwhile, the sulfated GLP presented remarkable but not dose-dependent inhibition on Heps hepatona in mice. With same degree of substitution, the sulfation protocol with aminosulfonic acid-pyridine yielded GLP sulfates with higher activity on HepG2 cells. In comparison, the native GLP showed no or little antitumor activity on the assayed cell lines but remarkable inhibition on suppressing the proliferation of rat Heps. The highest in vivo inhibition rate of 55.5% provided by sulfated GLP was observed on suppressing the proliferation of rat Heps. © 2011 Elsevier Ltd. All rights reserved. Source

Wen L.,Central China Normal University | Ke X.,Central China Normal University | Qiu L.,Jiangsu Institute of Nuclear Medicine | Zou Y.,Zhejiang Sci-Tech University | And 3 more authors.
Crystal Growth and Design | Year: 2012

Two microporous cadmium(II) metal-organic frameworks, [Cd(cptpy)(Ac)(H 2O)(DMA)(H 2O)] n (1) and [Cd(cptpy) 2(DMF) 2] n (2) (Hcptpy = 4-(4-carboxyphenyl)-2, 2′:4′,4′-terpyridine, DMA = N,N-dimethylacetamide, DMF = dimethylformamide) have been solvothermally synthesized under different reaction conditions. Complex 1 is a double-interpenetrating 3D network, while 2 is a noninterpenetrating (3,5)-connected 2D framework. The dehydrated forms of compounds 1 and 2 exhibit selective adsorption of CO 2 over N 2 and H 2O over CH 3OH. In addition, the adsorption value of CO 2 for 2 is higher than that of 1. The contents of uncoordinated pyridine nitrogen (Lewis basic sites) per formula unit of 1 and 2 are 2.16 and 4.36%, respectively. Obviously, the grafting of more uncoordinated pyridine nitrogen into compound 2 could enhance adsorption of the acidic CO 2 molecule. Notably, both 1 and 2 display strong photoluminescence. The nature of electronic transitions for complex 1 in the photoluminescent process was investigated by means of time-dependent density functional theory (TDDFT) calculations and molecular orbital analyses, which collaborates that the luminescent property is ligand-based. © 2012 American Chemical Society. Source

Guo W.-J.,Nanjing University | Zhang Y.-M.,Nanjing University | Zhang L.,Jiangsu Institute of Nuclear Medicine | Huang B.,Nanjing University | And 5 more authors.
Autophagy | Year: 2013

Failure to engage apoptosis appears to be a leading mechanism of resistance to traditional platinum drugs in patients with ovarian cancer. Therefore, an alternative strategy to induce cell death is needed for the chemotherapy of this apoptosis-resistant cancer. Here we report that autophagic cell death, distinct from cisplatin-induced apoptosis, is triggered by a novel monofunctional platinum (II ) complex named Mono-Pt in human ovarian carcinoma cells. Mono-Ptinduced cell death has the following features: cytoplasmic vacuolation, caspase-independent, no nuclear fragmentation or chromatin condensation, and no apoptotic bodies. These characteristics integrally indicated that Mono-Pt, rather than cisplatin, initiated a nonapoptotic cell death in Caov-3 ovarian carcinoma cells. Furthermore, incubation of the cells with Mono-Pt but not with cisplatin produced an increasing punctate distribution of microtubule-associated protein 1 light chain 3 (LC3), and an increasing ratio of LC3-II to LC3-I. Mono-Pt also caused the formation of autophagic vacuoles as revealed by monodansylcadaverine staining and transmission electron microscopy. In addition, Mono-Pt-induced cell death was significantly inhibited by the knockdown of either BECN1 or ATG7 gene expression, or by autophagy inhibitors 3-methyladenine, chloroquine and bafilomycin A1. Moreover, the effect of Mono-Pt involved the AKT1-MTOR-RPS6KB1 pathway and MAPK1 (ERK2)/MAPK3 (ERK1) signaling, since the MTOR inhibitor rapamycin increased, while the MAPK1/3 inhibitor U0126 decreased Mono-Pt-induced autophagic cell death. Taken together, our results suggest that Mono-Pt exerts anticancer effect via autophagic cell death in apoptosis-resistant ovarian cancer. These findings lead to increased options for anticancer platinum drugs to induce cell death in cancer. © 2013 Landes Bioscience. Source

Chronic rhinosinusitis (CRS) is one of the most common inflammatory diseases affecting large number of patients globally. The pathogenesis of CRS is still unclear and the treatment is unsatisfied. Clinical data provide the evidence that the chilblain-like alteration occurs in the early pathophysiology of CRS. We hypothesize that thermotherapy may offer a novel possible treatment strategy of chronic rhinosinusitis. In this article, we discuss the possibility of the chilblain-like alteration in the early pathophysiology and a therapeutic role of thermotherapy. © 2011 Elsevier Ltd. Source

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