Jiangsu Institute of Marine Resources

Xinpu, China

Jiangsu Institute of Marine Resources

Xinpu, China

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Liu Y.-W.,Huaihai Institute of Technology | Shi D.-H.,Jiangsu Institute of Marine Resources | Shi D.-H.,Huaihai Institute of Technology | Chen A.-J.,Peking Union Medical College | And 3 more authors.
Pharmaceutical Biology | Year: 2014

Context: To this day, there are no reports that marine compounds isolated from microorganisms of the Lianyungang area of China have been used for the treatment of Alzheimer's disease. Objective: The present study was to isolate fungi from the sea sediment of the Lianyungang area and screen for acetylcholineseterase inhibition activities of ethyl acetate extracts. Materials and methods: Fungi were isolated from the sea sediment and fermented. After centrifugation, the supernate was extracted with ethyl acetate. The ethyl acetate extract was then fractionated into five fractions. Acetylcholinesterase inhibition activities of the ethyl acetate extracts and five sub-fractions were tested at a concentration of 500μg/mL with the Ellman's method. Results: Forty-three marine fungi were isolated; 15 extracts inhibited acetylcholinestrease >50% and 3 extracts inhibited the acetylcholinesterase >80% at the concentration of 500μg/mL. The 3 extracts (L1705, S1101, SH0701) inhibited AChE dose-dependently with IC50 values of 11.3±1.2, 72.1±2.3, and 7.8±2.8μg/mL, respectively. After the extract of SH0701 was fractionated into five fractions, the ethyl acetate fraction possessed the highest acetylcholinesterase inhibitory activity with an inhibition rate of 71.55% at the concentration of 10μg/mL. The fungus SH0701 was identified as Aspergillus ochraceus SH0701 according to morphology and molecular identification. Discussion and conclusion: The present results indicates that some ethyl acetate extracts of marine fungi isolated from Lianyungang area of China could inhibit AChE potently. Therefore, some novel AChE inhibitors might exist in those extracts. © 2014 Informa Healthcare USA, Inc. All rights reserved.


Liu W.,Huaihai Institute of Technology | Liu W.,China University of Mining and Technology | Liu W.,Jiangsu Institute of Marine Resources | Li Q.,China University of Mining and Technology | And 3 more authors.
Chemistry Bulletin / Huaxue Tongbao | Year: 2014

As a natural amino monosaccharide and the final degradation product of chitin, D-glucosamine widely exists in a variety of organisms. There are one amino group and four hydroxyls in its molecule. Currently, D-glucosamine and its derivatives have attracted more attention due to its special nature of molecular structure, and the various products have been applied in many fields, such as biology, medicine and so on. Herein the chemical modification of D-glucosamine N-site and glycoside hydroxy was reviewed and the methods of selective chemical modification of D-glucosamine were especially introduced. Copyright © 2014.


Yin L.,Huaihai Institute of Technology | Cheng F.-C.,China University of Mining and Technology | Li Q.-X.,China University of Mining and Technology | Liu W.-W.,Huaihai Institute of Technology | And 6 more authors.
Journal of Chemical Research | Year: 2016

A new series of C1-glycosyl thiazole derivatives was synthesised by the reaction of 1-(1,3,4,6-tetra-O-acetyl-2-deoxy-β-D-glucopyranos-2-yl)thiourea with 2-bromoacetophenone derivatives. Subsequent removal of the acetyl groups were conducted using NaOMe-MeOH. The structures of all new products were confirmed by IR, 1H NMR and HRMS (ESI). The acetylcholinesterase inhibitory activities of these new compounds were tested. Among them, N-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-β-D-glucopyranosyl)-4-(4-nitrophenyl)-1,3-thiazole-2-amine showed the best activity with an inhibition rate of 43.21%. © 2016, Science Reviews 2000 Ltd. All rights reserved.


Shen X.,Huaihai Institute of Technology | Shen X.,Jiangsu Institute of Marine Resources | Meng X.,Huaihai Institute of Technology | Tian M.,Huaihai Institute of Technology | And 4 more authors.
Mitochondrial DNA | Year: 2015

The complete mitochondrial genome of Coelomactra antiquata (Guangxi, in China, GX) was determined. It is 16 801 bp in length and is the first representative from this province. The mitochondrial genome encodes 35 genes, including 12 PCGs, two ribosomal RNA, and 21 transfer RNA genes. Atp8 and trnSer(UCN) genes are missing, compared with the typical gene content of animal mitochondrial genomes. Three (cob, nad1, nad4, and nad6) of the 12 PCGs in the mitochondrial genome initiate with the ATA, while other PCGs start with ATG. Two PCGs (atp6 and nad4L) end with incomplete stop codons (T–), and the remaining ones have complete stop codons (TAA or TAG). The largest non-coding region of the C. antiquata (GX) contains one section of tandem repeats (5 × 99 bp). Among all PCGs and rRNAs, the nad5 gene contains the maximum polymorphic sites (430), followed by nad4 (261) and cox2 (240). Two ribosomal RNA genes (srRNA and lrRNA) and cox1 are most conservative. The proportions of polymorphic sites in six genes (nad4, nad2, nad6, nad5, cox2, and nad3) are more than 20% (ranging from 20.25% to 25.21%). These high variable genes can be used as molecular markers in the population genetic analysis of the species. © 2015 Taylor & Francis.


Guo L.,Huaihai Institute of Technology | Guo L.,Jiangsu Institute of Marine Resources | Zhu W.-C.,Huaihai Institute of Technology | Liu Y.-T.,Huaihai Institute of Technology | And 3 more authors.
Food Science and Biotechnology | Year: 2013

The optimized extraction conditions of total flavonoids from mimenghua were determined by the Box-Behnken design and response surface methodology. The optimal conditions to achieve the maximum yield of flavonoids were determined as follows: ethanol concentration 68%(v/v), extraction temperature 72.4°C, liquid/material ratio 48:1mL/g, and extraction time 2 h. Under the optimized conditions, the extraction ratio of flavonoids was 15.44%. The crude extract was then purified by AB-8 macroporous adsorption resin, the flavonoids content in the purified extract increased to 90.43%. The antioxidant activities of the purified flavonoids were evaluated in vitro by scavenging capabilities of DPPH radical, hydroxyl radical and superoxide radical, reducing power and total antioxidant capacity. The results showed that the flavonoids have significant antioxidant activities, which can be used as a source of potential antioxidant. © 2013 The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht.


Guo L.,Jiangsu Institute of Marine Resources | Guo L.,Huaihai Institute of Technology | Zhu W.,Huaihai Institute of Technology | Zheng A.,Huaihai Institute of Technology
Journal of Pure and Applied Microbiology | Year: 2014

To identify marine fungus SZX-6 isolated from the inner of Portunus triuberbuculatus with antibacterial activity and to optimize the fermentation conditions of producing antibacterial active substances. Based on its morphological characters and internal transcribed spacer (ITS) sequence analysis results, strain SZX-6 was identified as Aspergillus tubingensis. The optimized fermentation conditions were determined as sucrose 3%, beef extract 1.5%, KCl 0.1%, MgSO4 0.02%, old seawater, inoculum size 1%, 28°C, 160 r/min for 8 d by the the one-factor-at-a-time method, the orthogonal matrix method and the time-bioactivity cources assay. The initial identification data demonstrated that the antibacterial active substances were mainly composed of moderate polar components with plentiful quantity and alkaloid-colored. The strain of A. tubingensis being isolated from the seawater-cultured animal was firstly reported here, and its antimicrobial activity was initially invenstigated in this paper.


Xu X.H.,Huaihai Institute of Technology | Xu X.H.,Marine Innovation and Technology | Xu X.H.,Jiangsu Institute of Marine Resources | Zhu X.Y.,Huaihai Institute of Technology | And 4 more authors.
Journal of Crustacean Biology | Year: 2016

A full-length complementary DNA sequence of heat-shock protein 70 (HSP70) was cloned from the hepatopancreas of Charybdis japonica (A. Milne-Edwards, 1861) through reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. The expression profile of HSP70 in different tissues of C. japonica and its expression upon exposure to ammonia-N, nitrite-N, and sulfide was also investigated. The full-length cDNA of HSP70 was 2203 bp long and contained a 107 bp 5′ untranslated region (UTR), a 143 bp 3′ UTR, and a 1953 bp open reading frame, which distributed 108-2060 bp and encoded a polypeptide of 650 amino acids (aa) with a calculated molecular mass of 71.24 kDa and an isoelectric point of 5.34. Three signature sequences of the HSP70 family, IDLGTT-S-V (residues 9-18), IFDLGGGTFDVSIL (197-210 aa), and IVLVGGSTRIPKIQK (334-348 aa) were detected in the predicted amino-acid sequence. The HSP70 gene of C. japonica showed the highest similarity (>90%) to HSP70 from other crustacean species. The HSP70 gene was expressed in all tissues tested, and its abundance in these tissues can be arranged as hepatopancreas > stomach > gill > heart > ova > muscle. The expression of HSP70 was significantly induced after the stress of ammonia-N, nitrite-N, and sulfide, and the induced degree showed a positively correlation with increasing concentrations. The HSP70 expression reached a peak after 3 or 6 h of stress, which suggests that the HSP70 expression was affected quickly by the changes of environmental factors. © 2016 Copyright 2016 by The Crustacean Society. Published by Brill NV, Leiden.


Dai H.,Jiangsu Institute of Marine Resources | Dai H.,Huaihai Institute of Technology | Yang Y.,Huaihai Institute of Technology | Li C.,Huaihai Institute of Technology
2014 10th International Conference on Natural Computation, ICNC 2014 | Year: 2014

In order to overcome the premature convergence problem of clonal selection algorithm (CSA), a hybrid quantum crossover is proposed to improve the performance of CSA. The hybrid crossover is composed of two crossovers and well balance the exploration and exploitation search during immune maturation process. The effectiveness of the new crossover based CSA is evaluated on a class of traveling salesman problems (TSP). Simulation results demonstrate the performance of the proposed algorithm. © 2014 IEEE.


Guo L.,Jiangsu Institute of Marine Resources | Guo L.,Huaihai Institute of Technology | Liu Y.,Huaihai Institute of Technology | Liu C.,Huaihai Institute of Technology
Advance Journal of Food Science and Technology | Year: 2014

The mollusk Cyclina sinensis has been widely used as a food and folk medicine in China, Korea and south eastern Asia. In this study, Total Water-soluble Extracts (TWE), Crude Polysaccharides (CPS), Total Crude Proteins (TCP) were isolated from C. sinensis and the Deproteinized Polysaccharides (DPS) was obtained after deproteination of CPS. Their contents, such as neutral carbohydrate, protein and uronic acid, were measured. Their antioxidative properties against 1, 1'-Diphenyl-2-Picrylhydrazyl (DPPH) free radical, hydroxyl free radical and superoxide anion free radical, as well as their reducing capacity were investigated. Results showed that various fractions of C. sinensis can scavenge all free radicals tested above and possessed the increasing reducing capacity in a dose-dependent mode. Compared with the other three fractions, TWE represents the comprehensively best antioxidative properties. The antioxidative activities of TWE from C. sinensis might contribute to antioxidant-related functional food and pharmaceutical industries. © Maxwell Scientific Organization, 2014.


Zhu C.,Jiangsu Institute of Marine Resources | Zhu C.,University of Newcastle | Dong X.,PLA University of Science and Technology | Chen Z.,University of Newcastle | Naidu R.,University of Newcastle
International Journal of Environmental Science and Technology | Year: 2016

Amorphous tin(VI) hydrogen phosphate (ATHP) was synthesized using the liquid phase precipitation method and served as an adsorbent to remove Pb(II), Cu(II), and Zn(II) from aqueous solutions. The ATHP was characterized by powder X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and nitrogen adsorption–desorption techniques. Adsorption properties were evaluated as a function of pH, reaction time, concentration of reactants, and salinity. Their equilibrium adsorption data were modeled using Freundlich, Langmuir, and Dubinin–Kaganer–Radushkevich isotherms, respectively. The results revealed that adsorption equilibrium reached within 180 min. ATHP indicated good adsorption even below the pHZPC, and best adsorption at pH 5 for Pb(II) and Cu(II) and at pH 5.5 for Zn(II) was observed. Equilibrium data fitted better to the Langmuir model for Pb(II) and Cu(II) and fitted better to the Freundlich model for Zn(II). The saturated adsorption capacities deduced from the Langmuir model were 2.425, 1.801, and 0.600 mmol/g for Cu(II), Pb(II), and Zn(II), respectively, indicating an adsorption affinity order of Cu > Pb > Zn. There is a negative correlation between the concentration of NaCl and adsorption capacity of ATHP, yet ATHP still exhibited excellent adsorption having an adsorption capacity of 19.35, 15.16, 6.425 mg/g when the concentration of NaCl was 0.6 mol/L. The free energy (E) was 12.33, 10.70, and 14.74 kJ/mol for Pb(II), Cu(II), and Zn(II), respectively. An adsorption mechanism based on ion exchange between heavy metal ions and H+ in the ATHP is proposed. Furthermore, the used ATHP was regenerated by HCl solution and the adsorbent was used repeatedly. © 2016, Islamic Azad University (IAU).

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