Jiangsu Province Geriatric Institute

Nanjing, China

Jiangsu Province Geriatric Institute

Nanjing, China
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PubMed | Jiangsu Province Geriatric Institute and Jinling Hospital
Type: | Journal: International journal of rheumatic diseases | Year: 2016

Osteoarthritis (OA) is the most common chronic joint disease. This study aimed to uncover underlying mechanisms of OA pathogenesis and explore the potential biomarkers of osteoarthritic acetabular labrum.The microarray data GSE60762 was utilized, containing five OA acetabular labrum samples and three healthy control samples. Data were preprocessed by oligo package and the differentially expressed genes (DEGs) were identified using limma package with predefined criteria, followed by functional enrichment analysis by the GoFunction in R Bioconductor, and protein-protein interaction (PPI) network analysis.As a result, 141 DEGs (44 were up-regulated and 97 were down-regulated) were identified between OA and healthy acetabular labrum cells. Up-regulated genes including CDH2 and WNT5A were significantly enriched in intracellular signal transduction function, while down-regulated genes such as KDR, FLT1 and CDH5 were remarkably correlated with cardiovascular system development. FLT1, KDR, CDH2 and CDH5 were the striking nodes in the PPI network.CDH2, WNT5A, KDR, FLT1 and CDH5 might serve as the biomarkers of OA prognosis. Intracellular signal transduction and cardiovascular system development might play significant roles in the destruction of labrum during OA progression. However, more experimental validations are warranted to confirm our findings.

Gu X.,Jiangsu University | Fu M.,Jiangsu University | Ge Z.,Jiangsu University | Zhan F.,Jiangsu University | And 11 more authors.
Scientific Reports | Year: 2015

Melanoma-associated antigens (MAGE)-A9 has been reported to play important roles in the development of human cancers. However, the association between MAGE-A9 expression and the clinicopathological characteristics of hepatocellular carcinoma (HCC) is not well understood. The study was to detect the expression of MAGE-A9 in human HCC and investigate the association between its expression and the clinicopathological characteristics of HCC. Reverse transcription-polymerase chain reaction (RT-PCR), one-step quantitative -PCR (qPCR) and immunohistochemistry (IHC) analyses were performed to characterize the expression of MAGE-A9 in HCC cell lines and tissues. Kaplan-Meier survival and Cox regression analyses were employed to evaluate the prognosis of 100 HCC patients. The results showed that the expression of MAGE-A9 in HCC was significantly higher than that in non-cancerous cells and tissues. Moreover, the expression level of the MAGE-A9 protein in HCC was related to the pathological grade (p = 0.003), portal vein invasion (p = 0.001), distant metastasis (p = 0.022) and TNM stage (p = 0.005). Cox regression analysis further revealed that MAGE-A9 expression is an independent prognostic factor for disease-free survival (p = 0.006) and overall survival (p = 0.022). These data are the first to indicate that MAGE-A9 expression is a valuable prognostic biomarker for HCC and that high MAGE-A9 expression suggests unfavorable survival outcomes in HCC patients.

PubMed | Jiangsu Province Geriatric Institute, Shanhai Meishan Hospital and Nanjing Medical University
Type: | Journal: Clinical neurology and neurosurgery | Year: 2016

A longitudinal (30-month) study of the cognitive changes in Parkinsons disease patients and analysis of influencing factors.The cognitive function and related symptoms of 102 patients with idiopathic Parkinsons disease were assessed using the Montreal Cognitive Assessment (MoCA), Mini-Mental State Examination (MMSE), and relevant scales, at baseline and 30-month follow-up. The t-test, nonparametric tests, and regression analyses were used to evaluate cognitive decline and investigate risk factors for cognitive impairment.From baseline to follow-up, the MMSE and MoCA scores significantly decreased, respectively, from 28.162.29 to 26.183.64, and from 24.604.23 to 21.945.47 (both P<0.001). Impairment was observed in multiple cognitive areas, significantly in naming, delayed recall, and orientation (P<0.01). Patients at baseline with postural instability and gait disturbance (PIGD), lower MoCA scores, or depression had a higher risk of cognitive impairment at follow-up (P<0.01).Cognitive impairment is highly prevalent in Parkinsons disease patients, especially for those with lower MoCA scores, PIGD, and depression.

PubMed | Peoples Hospital of Jiangsu Province and Jiangsu Province Geriatric Institute
Type: Journal Article | Journal: Experimental and molecular pathology | Year: 2016

Osteosarcoma (OS) is the most common primary bone cancer, and it is most prevalent in children and young adults. The prognosis of OS remains poor, and survival of OS reached a plateau. The discovery of microRNAs (miRNAs) provides a new possibility for the early diagnosis and treatment of OS. In this study, we detected the expression level of miR-205 and Transforming growth factor-alpha (TGF-) in 15 cases of clinical OS tissues and adjacent normal bone tissues. We found that the expression of miR-205 was significantly lower in OS tissues than in normal bone tissues; the expression of TGF- mRNA was significantly increased in OS tissues than in normal bone tissues, the miR-205 was negatively correlated with TGF- levels in both OS and normal bone tissues. Functional studies demonstrated that miR-205 significantly decreased the capability of cell proliferation, invasion and migration and induced G0/G1 growth arrest and apoptosis in OS cells. By using bioinformatics analytic tool (Targetscan), the 3UTR of TGF- gene was found to be a target of miR-205. Luciferase report assay further confirmed that TGF- 3UTR is a direct target of miR-205. We also found that the expression of TGF- mRNA and protein was significantly down-regulated or up-regulated after miR-205 mimic or miR-205 inhibitor transfection. TGF- knockdown study further showed that miR-205 regulated cell proliferation, invasion and migration by targeting TGF- in OS. Enforced expression of TGF- sufficiently restore the effects of miR-205 on cell proliferation, invasion and migration. In conclusion, our study suggested that miR-205 may function as a tumor suppressor via targeting TGF- in OS, and the abnormal expression of miR-205 might be a key factor in OS progression.

Ma J.,Wannan Medical College | Wang J.,Jiangsu Province Geriatric Institute | Fan W.,Jiangsu Province Geriatric Institute | Pu X.,Jiangsu Province Geriatric Institute | And 7 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2014

The tissue inhibitor of metalloproteinase-1 (TIMP-1) is an endogenous inhibitor of matrix metalloproteinases and potential biomarker of various types of human cancers. However, the association between TIMP-1 expression and the clinical features of laryngeal squamous cell carcinoma (LSCC) is barely investigated. In this study, one-step quantitative reverse transcription-polymerase chain reaction and immunohistochemical staining with tissue microarrays were employed to evaluate the relationship between TIMP-1 expression and the clinicopathological characteristics of LSCC. Results showed that the TIMP-1 mRNA and protein expression levels were significantly higher in LSCC than in the corresponding non-cancerous tissues (p<0.05). TIMP-1 protein expression in LSCC was associated with tumor differentiation (p=0.012) and overall survival (p=0.043). Kaplan-Meier method and Cox multi-factor analysis suggested that high TIMP-1 expression (p=0.008) and positive lymph node metastasis (p=0.029) were significantly associated with the poor survival of patients with LSCC. These data indicated that TIMP-1 may be identified as a prognostic marker of LSCC.

PubMed | Jiangsu Province Geriatric Institute, Nanjing Medical University and Queen Mary, University of London
Type: Journal Article | Journal: Oncotarget | Year: 2016

MicroRNAs (miRNAs) are short, conserved segments of non-coding RNA which play a significant role in prostate cancer development and progression. To identify miRNAs associated with castration resistance, we performed miRNA microarray analysis comparing castration resistant prostate cancer (CRPC) with androgen dependent prostate cancer (ADPC). We identified common underexpression of miR-4638-5p in CRPC compared to ADPC samples, which were further confirmed by quantitative PCR analysis. The role of miR-4638-5p in prostate cancer androgen-independent growth has been demonstrated both in vitro and in vivo. We also identified Kidins220 as a target gene directly regulated by miR-4638-5p and shRNA-mediated knockdown of Kidins220 phenocopied miR-4638-5p restoration. Subsequently, we revealed that Kidins220 activates PI3K/AKT pathway, which plays a key role in CRPC. Loss of miR- 4638-5p may lead to CRPC through the activity of Kidins220 and PI3K/AKT pathway. Furthermore, we found that miR-4638-5p, through regulating Kidins220 and the downstream activity of VEGF and PI3K/AKT pathway, influences prostate cancer progression via angiogenesis. The identification of miR-4638-5p down-regulation in CRPC and the understanding of the functional role of miR-4638-5p and its downstream genes/pathways have the potential to develop biomarkers for CRPC onset and to identify novel targets for novel forms of treatments of this lethal form of PCa.

Fu M.,Jiangsu University | Fan W.,Jiangsu Province Geriatric Institute | Pu X.,Jiangsu Province Geriatric Institute | Ni H.,Jiangsu University | And 6 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2013

SH2-containing inositol 5'-phosphatase 2 (SHIP2) is a vital regulator of phosphoinositide pools in metabolic pathways and is considered to downregulate phosphatidylinositol 3'-kinase signaling, which underlies the development of several kinds of human cancers. However, SHIP2 expression in non-small cell lung cancer (NSCLC) and its relationship with the clinical characteristics of NSCLC remain poorly understood. In this study, one-step quantitative reverse transcription-polymerase chain reaction and immunohistochemistry analysis with tissue microarray was used to evaluate SHIP2 expression in NSCLC and to investigate the relationship of this expression to NSCLC prognosis. Results showed that the expression of SHIP2 messenger RNA and protein was significantly higher in NSCLC than in corresponding non-cancerous tissues (both p < 0.05). SHIP2 protein expression in NSCLC was related to lymph node metastasis (p = 0.042), TNM stage (p = 0.036), and 5-year survival rate (p = 0.046). The Kaplan-Meier method and log-rank test suggested that high SHIP2 expression, tobacco consumption, and advanced tumor stage were significantly associated with low survival of NSCLC patients. The results of this research suggested that SHIP2 expression was correlated with malignant phenotypes of NSCLC and may thus serve as a poor prognostic factor and valuable oncogene for NSCLC.

Li Q.,Nanjing Medical University | Qiao D.,Jiangsu Province Geriatric Institute | Song N.-H.,Nanjing Medical University | Ding Y.,Jiangsu Province Geriatric Institute | And 4 more authors.
World Journal of Urology | Year: 2013

Purpose: To investigate the effect of the deleted in azoospermia (DAZ) copy cluster deletion on spermatogenesis in the South Chinese population. Methods: In this study, the prevalence and characteristics of different DAZ copy cluster deletions and their association with spermatogenic failure were analyzed. A total of 186 infertile men with different spermatogenic impairments and 190 normozoospermic fertile men were studied. Three DAZ-specific single nucleotide variant loci and seven AZFc-specific sequence-tagged sites were examined using polymerase chain reaction (PCR)-restriction fragment length polymorphism and routine PCR. Results: Gr/gr deletions were observed in a total of 9 of the 190 normozoospermic fertile men, and 11 gr/gr deletions were found in 186 infertile men. In addition, 3 b2/b3 deletions were identified in the infertile, but not in the fertile men. DAZ-SNV loci analysis revealed 4 DAZ copies that had 8 gr/gr-DAZ3/DAZ4 deletions and 1 gr/gr-DAZ1/DAZ2 deletion in the fertile men (8/190 vs. 1/190, p = 0.037). Analysis of DAZ deletion copies in infertile men revealed 10 gr/gr-DAZ1/DAZ2 deletions, 1 gr/gr-DAZ3/DAZ4 deletion (10/186 vs. 1/186, p = 0.011) and 3 b2/b3-DAZ1/DAZ2 deletions (13/186 vs. 1/186, p = 0.002). Conclusions: Analysis of DAZ gene copies in AZFc microdeletions suggests that the contribution of the different deletions to male infertility varies. Removing DAZ1/DAZ2 seems to be associated with spermatogenic impairment, whereas removing DAZ3/DAZ4 seems to have little or no effect on fertility in the South Chinese population. © 2013 Springer-Verlag Berlin Heidelberg.

Wang J.,Jiangsu Province Geriatric Institute | Bian R.-W.,Jiangsu Province Geriatric Institute | Mo Y.-Z.,Jiangsu Province Geriatric Institute
Journal of Clinical Gerontology and Geriatrics | Year: 2013

Background/Purpose The aim of the present study is to validate the Chinese version 8-item Morisky medication adherence scale (MMAS-8) in patients with type 2 diabetes mellitus (T2DM). Methods A cross-sectional survey was conducted. After translation, a convenience sample of 182 patients with T2DM complete the Chinese version MMAS-8, and medication adherence visual analogue scale. The intraclass correlation coefficient and Cronbach α were calculated to determine reliability and internal consistency, respectively. Validity was confirmed using convergent, known group, and construct validity. Results The internal consistency determined by Cronbach α was 0.65. Test-retest reliability expressed by intraclass correlation coefficient was 0.80. A positive correlation was observed between Chinese version MMAS-8 and medication adherence visual analogue scale (r = 0. 75, p < 0.01). A significant relationship between MMAS-8 categories and HbA1c categories (χ2 = 21.63; p < 0.001) was found. Factor analysis showed that the MMAS had two dimensions: forgetting to take medications and the complexity of drug regimen; and stopping medication. Conclusion The Chinese version of the MMAS-8 is a reliable and valid measure of medication adherence that can now be used in type 2 diabetic patients. Copyright © 2013, Asia Pacific League of Clinical Gerontology & Geriatrics. Published by Elsevier Taiwan LLC. All rights reserved.

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