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Du Z.D.,Yangzhou University | Huang X.G.,Jiangsu Engineering Research Center for Molecular Breeding of Pig | Wu S.L.,Jiangsu Engineering Research Center for Molecular Breeding of Pig | Bao W.B.,Jiangsu Engineering Research Center for Molecular Breeding of Pig
Journal of Animal and Veterinary Advances | Year: 2011

The Bactericidal/Permeability-Increasing protein (BPI) plays a very important role in the animal body's natural defense. It has a series of biological functions such as killing Gram-negative bacteria, the neutralization of endotoxin or lipopolysaccharides, etc. To investigate the effect and mechanism of porcine BPI gene on enterotoxigenic Escherichia coli F18, particularly whether the expression of BPI gene is related to the different breeds. In this study, real-time PCR was used to analyze the expression oiBPI gene expression in post-weaning piglets of Yorkshire, Sutai (Resource Population of resistance toE. coli Fl 8) and Meishan. The results showed that there was almost no expression or very low expression of BPI gene in heart, liver, spleen, lung, kidney, stomach, muscle, thymus and lymph nodes but there was a high expression in the duodenum and jejunum and the expression levels were significantly different from those of the other 9 organs. It also showed that the jejunum and duodenum expression of BPI gene in Sutai was significant higher than Yorkshire and Meishan (p<0.05). The researchers can speculate that the expression of BPI gene was significant difference in different breeds. The researchers can further indicates that the expression of the BPI gene in Sutai piglets could be beneficial to the infection of E. coli. BPI gene might have a direct role against diarrhea and edema disease caused by ECF18 in weaned piglets, the resistance was related to the upregulation of BPI gene expression in the intestine. BPI gene can be identified as a genetic marker for future breeding againstii. coli and Salmonella related diseases. © Medwell Journals, 2011.

Bao W.B.,Yangzhou University | Ye L.,Yangzhou University | Pan Z.Y.,Yangzhou University | Zhu J.,Yangzhou University | And 4 more authors.
Animal Genetics | Year: 2012

In this study, Agilent two-colour microarray-based gene expression profiling was used to detect differential gene expression in duodenal tissues collected from eight full-sib pairs of Sutai pigs differing in adhesion phenotype (sensitivity and resistance to Escherichia coli F18). Using a two-fold change minimum threshold, we found 18 genes that were differentially expressed (10 up-regulated and eight down-regulated) between the sensitive and resistant animal groups. Our gene ontology analysis revealed that these differentially expressed genes are involved in a variety of biological processes, including immune responses, extracellular modification (e.g. glycosylation), cell adhesion and signal transduction, all of which are related to the anabolic metabolism of glycolipids, as well as to inflammation- and immune-related pathways. Based on the genes identified in the screen and the pathway analysis results, real-time PCR was used to test the involvement of ST3GAL1 and A genes (of glycolipid-related pathways), SLA-1 and SLA-3 genes (of inflammation- and immune-related pathways), as well as the differential genes FUT1, TAP1 and SLA-DQA. Subsequently, real-time PCR was performed to validate seven differentially expressed genes screened out by the microarray approach, and sufficient consistency was observed between the two methods. The results support the conclusion that these genes are related to the E. coli F18 receptor and susceptibility to E. coli F18. © 2011 The Authors, Animal Genetics © 2011 Stichting International Foundation for Animal Genetics.

Bao W.B.,Yangzhou University | Bao W.B.,Jiangsu Engineering Research Center for Molecular Breeding of Pig | Ye L.,Yangzhou University | Pan Z.Y.,Yangzhou University | And 6 more authors.
Czech Journal of Animal Science | Year: 2011

The genetic variation in exon 1 of the TLR4 gene was detected among a total of 893 animals, including Asian wild boars, 3 imported commercial and 10 Chinese indigenous pig breeds. The expression of TLR4 was assayed by RT-PCR and different expression between resistant and sensitive resource populations to ETEC F18 was analysed to discuss the role that the TLR4 gene plays in resistance. In this study, new alleles were detected in exon 1 of the TLR4 gene. These polymorphisms are significantly different between Chinese indigenous breeds and imported breeds. Based on the published TLR4 gene sequence (AB232527) in GenBank, a 93G > C mutation was found in 5'UTR and only a 194G > A synonymous mutation was found in the coding sequence of exon 1. In addition, TLR4 gene was broadly expressed in 11 tissues with the highest level in lung. The expression was relatively high in the lymph nodes, kidney and spleen. Generally, the expression of TLR4 gene in sensitive individuals was higher than that in resistant individuals. The results indicated that the downregulation of the mRNA expression of TLR4 gene had reduced the transmembrane signal transduction of LPS and then led to the responsive ability of the host to ETEC F18 in piglets.

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